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A member of CGIAR consortium www.iita.org
Breeding Staple Crops
R. Asiedu
A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium
IITA’s Strategic Research for Development Goals
1. Increase major staple food crop (cassava, yam, maize,
banana/plantain, soybean, and cowpea) yields by 60%
2. Increase average farm income by 50%, lifting 25% of poor
households above the poverty line (over 11 million Africans)
3. Reduce the number of malnourished children by 30%
4. Restore 40% of farms to sustainable resource management
(revitalizing over 7.5 million hectares of degrading
farmlands)
R4D Goals
A member of CGIAR consortium www.iita.org
A member of CGIAR consortium www.iita.org
http://genebank.iita.org/
International collections of
major food crops in Africa
Crop
Number of
accessions
Cowpea (Vigna unguiculata L.) 15379
Soybean (Glycine max L. Merr) 4841
Cassava (Manihot esculenta
Crantz)
3499
Yam (Dioscorea spp.) 3170
Bambara groundnut (Vigna
subterranea L. Verdc)
1752
Maize (Zea mays L.) 1565
Miscellaneous legumes 558
Wild Vigna (Vigna species L.) 1543
Banana/plantain (Musa spp.) 546
African yam bean
[Sphenostylis stenocarpa
(Hochst.) Harms]
456
A member of CGIAR consortium www.iita.org
Descriptors for Germplasm
Characterization
Website:
http://cropgenebank.sgrp.cgiar.org/index.php?option=com_content&view=article&id=387&Item
A member of CGIAR consortium www.iita.org
Microscopic chromosome
images at different stages in a
cell division cycle of TDr
95/18544
1: Interphase, 2: Prophase, 3:
Metaphase, 4: Anaphase, 5:
Telophase
1 2
4 5
31 2
4 5
3
Ploidy determination
Source: Vroh, 2008
DNA flow cytometry is used to overcome
some of the challenges of chromosome
counting
• the method is non-destructive, rapid,
sensitive and convenient
• does not require dividing cells
A member of CGIAR consortium www.iita.org
Genetic diversity tree of 342 yam accessions from the core
collection based
on SSR data using unweighted neighbour- joining analysis
D. alata (red)
D. bulbifera (purple)
D. cayenensis (yellow)
D. dumetorum (ash)
D. esculenta (blue)
D. rotundata (green)
-Results generally consistent with
established taxonomical relationships
-High level of genetic variation in core
germplasm
Source: Kolesnikova & Vroh, 2008
A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium
Key traits:
Early maturity
High promiscuous nodulation
Resistance to rust, leaf spot and bacterial
pustule
High grain and fodder yield
Shattering resistance
Good seed storability
Soybean
A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium
Key traits:
High grain yield
Drought tolerance
Adaptation to sub-optimal soil nitrogen
Tolerance to lodging
Resistance to major foliar diseases and maize
streak virus
Maturity (late, medium, early, or extra early)
Tolerance to Striga hermonthica
Resistance to stem borers (Sesamia calamistis and
Eldana sacharina)
High pro-vitamin A content
High lysine and tryptophan contents
Resistance to aflatoxin contamination
Maize
A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium
Key traits:
High and stable root yield
High root dry matter (starch) content
Drought tolerance
High root pro-vitamin A content
Early maturity
Low cyanogenic potential of roots
Disease resistance (cassava mosaic, anthracnose,
bacterial blight)
Cassava
A member of CGIAR consortium www.iita.org
Breeding for Increased Nutrient
Density – HarvestPlus Project
A member of CGIAR consortium www.iita.org
Product Delivery Schedule
Target products
Target pro-vitamin A content (µg/g)
Planned year
of release
1st Generation 6–8 µg/g (40–60% target increase) 2012
2nd Generation 8–12 µg/g (60–80% target increase) 2015
3rd Generation ≥15 µg/g (≥100% target increase) 2017-2018
A member of CGIAR consortium www.iita.org
Mean pro-
vitamin A
Mean pro-
vitamin A
Mean grain
yield
Mean grain
yield
2009 2011 2010 2011
Hybrids (μg/g) (μg/g) (kg/ha) (kg/ha)
Ife maizehyb-3 8.8 7.2 5440 5744
Ife maizehyb-4 8.1 7.4 5121 5198
Oba Super 2 (CH) 5.3 3.5 4636 4867
Mean 5.8 5.2 5026 4889
LSD(0.05) 0.7 1.0 1093 673
CV 13 16 15 16
Mean pro-vitamin A content and grain yields of hybrids released in Nigeria in
2012.
Regional trials: Sources of Vitamin A rich hybrids for release
A member of CGIAR consortium www.iita.org
Provitamin A content and agronomic performance of promising OPVs and hybrids included in regional
trials
Regional Trials: Channelling promising provitamin A enriched
OPVs and hybrids to partners for testing
A member of CGIAR consortium www.iita.org
Genetic Gains in Grain Yield
- DTMA Project
A member of CGIAR consortium www.iita.org
Grain yield (kg ha-1
) of three breeding eras of early maize
cultivars evaluated under multiple stress and non stress
environments in Benin, Ghana, and Nigeria, 2010 and 2011.
Environment
Era % Genetic
gain Era-1
1 (1988-2000)
15 cvs
2 (2001-2006)
16 cvs
3 (2007-2010)
19 cvs
Multistress
(16 env)
2176 2286 2606 11.16
Non-stress
(35 env)
3398 3615 3957 9.02
Mean 2760 2909 3207 8.88
A member of CGIAR consortium www.iita.org
Grain yield (kg ha-1
) of three breeding eras of extra-early maize
cultivars under multiple stress and non stress environments in
Nigeria, 2013.
Era
% Genetic
gain Era-1
Environment 1 (1995-2000) 2 (2001-2006) 3 (2007-2012)
12 cvs 19 cvs 25 cvs
Multistress
(11 env)
1786 1989 2248 15.2
Non-stress
(16 env)
2714 2976 3354 13.9
Mean 2250 2483 2802 11.2
A member of CGIAR consortium www.iita.org
Released early and extra-early maize hybrids and OPVs in
DTMA Partner Countries, 2007-2015.
Country Number of
varieties
released
Number
of
hybrids
released
Year of
release
Institution Total
Benin 6 0 2007-2015 INRAB/IITA 6
Ghana 5 2 2010-2012 CRI/SARI/IITA 7
Mali 2 7 2009-2014 IER/IITA 9
Nigeria 6 4 2009-2013 IAR/IAR&T/IITA 10
Total 19 13 2007-2014 32
A member of CGIAR consortium www.iita.org
Two extra-early maturing (80-85 days to maturity) white hybrids released in
Nigeria in 2013 in collaboration with IAR&T
Ife Hybrid 5 and Ife Hybrid 6 combine drought and low N tolerance with
Striga resistance.
Will contribute to more stable maize production in in the face of increasingly
unpredictable rainfall.
Striga tolerant versus susceptible
hybrids
Drought tolerant versus susceptible
hybrids
A member of CGIAR consortium www.iita.org
Impact of Soybean RustImpact of Soybean Rust
10 g seeds = 196 (D), 89 (H)
0 7
1418
• Premature
defoliation
• 60-80% grain
yield loss
• Seed size
reduced
• HPR ideal for
African farmers
Source: Bandyopahyay, 2010
A member of CGIAR consortium www.iita.org
Resistant Susceptible
Rust Resistance Evaluation in Checkerboard Design
Source: Bandyopahyay, 2010
A member of CGIAR consortium www.iita.org
Attacks flowers and pods of various legumes, up to 80% yield loss
An old enemy: the legume pod borer,
Maruca vitrata
A member of CGIAR consortium www.iita.org
Severely
affected
roots are
unfit for
any use
Cassava Brown Streak Disease
A member of CGIAR consortium www.iita.org
A member of CGIAR consortium www.iita.org

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Breeding Staple Crops

  • 1. A member of CGIAR consortium www.iita.org Breeding Staple Crops R. Asiedu
  • 2. A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium IITA’s Strategic Research for Development Goals 1. Increase major staple food crop (cassava, yam, maize, banana/plantain, soybean, and cowpea) yields by 60% 2. Increase average farm income by 50%, lifting 25% of poor households above the poverty line (over 11 million Africans) 3. Reduce the number of malnourished children by 30% 4. Restore 40% of farms to sustainable resource management (revitalizing over 7.5 million hectares of degrading farmlands) R4D Goals
  • 3. A member of CGIAR consortium www.iita.org
  • 4. A member of CGIAR consortium www.iita.org http://genebank.iita.org/ International collections of major food crops in Africa Crop Number of accessions Cowpea (Vigna unguiculata L.) 15379 Soybean (Glycine max L. Merr) 4841 Cassava (Manihot esculenta Crantz) 3499 Yam (Dioscorea spp.) 3170 Bambara groundnut (Vigna subterranea L. Verdc) 1752 Maize (Zea mays L.) 1565 Miscellaneous legumes 558 Wild Vigna (Vigna species L.) 1543 Banana/plantain (Musa spp.) 546 African yam bean [Sphenostylis stenocarpa (Hochst.) Harms] 456
  • 5. A member of CGIAR consortium www.iita.org Descriptors for Germplasm Characterization Website: http://cropgenebank.sgrp.cgiar.org/index.php?option=com_content&view=article&id=387&Item
  • 6. A member of CGIAR consortium www.iita.org Microscopic chromosome images at different stages in a cell division cycle of TDr 95/18544 1: Interphase, 2: Prophase, 3: Metaphase, 4: Anaphase, 5: Telophase 1 2 4 5 31 2 4 5 3 Ploidy determination Source: Vroh, 2008 DNA flow cytometry is used to overcome some of the challenges of chromosome counting • the method is non-destructive, rapid, sensitive and convenient • does not require dividing cells
  • 7. A member of CGIAR consortium www.iita.org Genetic diversity tree of 342 yam accessions from the core collection based on SSR data using unweighted neighbour- joining analysis D. alata (red) D. bulbifera (purple) D. cayenensis (yellow) D. dumetorum (ash) D. esculenta (blue) D. rotundata (green) -Results generally consistent with established taxonomical relationships -High level of genetic variation in core germplasm Source: Kolesnikova & Vroh, 2008
  • 8. A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium Key traits: Early maturity High promiscuous nodulation Resistance to rust, leaf spot and bacterial pustule High grain and fodder yield Shattering resistance Good seed storability Soybean
  • 9. A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium Key traits: High grain yield Drought tolerance Adaptation to sub-optimal soil nitrogen Tolerance to lodging Resistance to major foliar diseases and maize streak virus Maturity (late, medium, early, or extra early) Tolerance to Striga hermonthica Resistance to stem borers (Sesamia calamistis and Eldana sacharina) High pro-vitamin A content High lysine and tryptophan contents Resistance to aflatoxin contamination Maize
  • 10. A member of CGIAR consortium www.iita.orgwww.iita.orgA member of CGIAR consortium Key traits: High and stable root yield High root dry matter (starch) content Drought tolerance High root pro-vitamin A content Early maturity Low cyanogenic potential of roots Disease resistance (cassava mosaic, anthracnose, bacterial blight) Cassava
  • 11. A member of CGIAR consortium www.iita.org Breeding for Increased Nutrient Density – HarvestPlus Project
  • 12. A member of CGIAR consortium www.iita.org Product Delivery Schedule Target products Target pro-vitamin A content (µg/g) Planned year of release 1st Generation 6–8 µg/g (40–60% target increase) 2012 2nd Generation 8–12 µg/g (60–80% target increase) 2015 3rd Generation ≥15 µg/g (≥100% target increase) 2017-2018
  • 13. A member of CGIAR consortium www.iita.org Mean pro- vitamin A Mean pro- vitamin A Mean grain yield Mean grain yield 2009 2011 2010 2011 Hybrids (μg/g) (μg/g) (kg/ha) (kg/ha) Ife maizehyb-3 8.8 7.2 5440 5744 Ife maizehyb-4 8.1 7.4 5121 5198 Oba Super 2 (CH) 5.3 3.5 4636 4867 Mean 5.8 5.2 5026 4889 LSD(0.05) 0.7 1.0 1093 673 CV 13 16 15 16 Mean pro-vitamin A content and grain yields of hybrids released in Nigeria in 2012. Regional trials: Sources of Vitamin A rich hybrids for release
  • 14. A member of CGIAR consortium www.iita.org Provitamin A content and agronomic performance of promising OPVs and hybrids included in regional trials Regional Trials: Channelling promising provitamin A enriched OPVs and hybrids to partners for testing
  • 15. A member of CGIAR consortium www.iita.org Genetic Gains in Grain Yield - DTMA Project
  • 16. A member of CGIAR consortium www.iita.org Grain yield (kg ha-1 ) of three breeding eras of early maize cultivars evaluated under multiple stress and non stress environments in Benin, Ghana, and Nigeria, 2010 and 2011. Environment Era % Genetic gain Era-1 1 (1988-2000) 15 cvs 2 (2001-2006) 16 cvs 3 (2007-2010) 19 cvs Multistress (16 env) 2176 2286 2606 11.16 Non-stress (35 env) 3398 3615 3957 9.02 Mean 2760 2909 3207 8.88
  • 17. A member of CGIAR consortium www.iita.org Grain yield (kg ha-1 ) of three breeding eras of extra-early maize cultivars under multiple stress and non stress environments in Nigeria, 2013. Era % Genetic gain Era-1 Environment 1 (1995-2000) 2 (2001-2006) 3 (2007-2012) 12 cvs 19 cvs 25 cvs Multistress (11 env) 1786 1989 2248 15.2 Non-stress (16 env) 2714 2976 3354 13.9 Mean 2250 2483 2802 11.2
  • 18. A member of CGIAR consortium www.iita.org Released early and extra-early maize hybrids and OPVs in DTMA Partner Countries, 2007-2015. Country Number of varieties released Number of hybrids released Year of release Institution Total Benin 6 0 2007-2015 INRAB/IITA 6 Ghana 5 2 2010-2012 CRI/SARI/IITA 7 Mali 2 7 2009-2014 IER/IITA 9 Nigeria 6 4 2009-2013 IAR/IAR&T/IITA 10 Total 19 13 2007-2014 32
  • 19. A member of CGIAR consortium www.iita.org Two extra-early maturing (80-85 days to maturity) white hybrids released in Nigeria in 2013 in collaboration with IAR&T Ife Hybrid 5 and Ife Hybrid 6 combine drought and low N tolerance with Striga resistance. Will contribute to more stable maize production in in the face of increasingly unpredictable rainfall. Striga tolerant versus susceptible hybrids Drought tolerant versus susceptible hybrids
  • 20. A member of CGIAR consortium www.iita.org Impact of Soybean RustImpact of Soybean Rust 10 g seeds = 196 (D), 89 (H) 0 7 1418 • Premature defoliation • 60-80% grain yield loss • Seed size reduced • HPR ideal for African farmers Source: Bandyopahyay, 2010
  • 21. A member of CGIAR consortium www.iita.org Resistant Susceptible Rust Resistance Evaluation in Checkerboard Design Source: Bandyopahyay, 2010
  • 22. A member of CGIAR consortium www.iita.org Attacks flowers and pods of various legumes, up to 80% yield loss An old enemy: the legume pod borer, Maruca vitrata
  • 23. A member of CGIAR consortium www.iita.org Severely affected roots are unfit for any use Cassava Brown Streak Disease
  • 24. A member of CGIAR consortium www.iita.org
  • 25. A member of CGIAR consortium www.iita.org