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Biosecurity in the practice
Olivier Simon, DMV, Dip ECVS
The University of Adelaide – EH&PC
Suspected case of contagious
infectious respiratory disease
• Clinical signs
– Fever
– Cough
– Swollen lymph nodes (lymphadenopathy)
– Abnormal nasal discharge
– Abnormal respiratory sounds
– Urticaria, limb edema
• Fever presenting alone should be considered as 
suspected contagious respiratory disease
• Absence of fever, in the presence of respiratory clinical 
signs, does not rule out contagious infectious disease
Suspected case of contagious
infectious respiratory disease
• Possible primary diagnoses for respiratory 
disease with herd health implications:
– Equine Herpes virus‐1 (EHV‐1)
– Equine Herpes virus‐4 (EHV‐4)
– Streptococcus equi equi (strangles)
– Hendra virus (emerging)
– Equine influenza (EI) (exotic)
– Equine viral arteritis (EVA) (exotic)
Suspected case of infectious 
respiratory disease
Establish biosecurity 
perimeter
Communicate plan Attempt diagnosis
Identify primary 
perimeter
Identify secondary 
perimeter
Increase disease 
surveillance
Regulate horse 
movement
Implement primary 
perimeter
Stop horse 
movement
Limit human 
movement
Biosecurity
Guidelines
Veterinarians
Horsemen
Regulatory agencies
media
Related industry 
affiliates
Event management
Sample collection
tests
Laboratory selection
Testing applies
Sample handling
Sample transport
No diagnosis
Maintain biosecurity
21‐28 days after last case
Expand differential 
diagnoses
Consult infectious 
disease expert
Diagnosis
Disease specific guidelines
Equine Herpes virus‐1
Influenza
Strep equi
Equine viral arteritisAAEP 2006
Suspected case of contagious
infectious respiratory disease
1. Establish a primary biosecurity perimeter
– Centered on the location of the contagious 
respiratory disease case(s)
– Extended until a clearly defined physical barrier to 
further spread of infection is identified
– May encompass the entire equine facility
Suspected case of contagious
infectious respiratory disease
1. Establish a primary biosecurity perimeter
– If site design permits, the perimeter may contain 
only part of the equine facility
– Include the isolation facility if one exists
– Signs are used to identify the perimeter & control 
access
Suspected case of contagious
infectious respiratory disease
• The primary perimeter contains all suspected infected 
animals & animals in immediate contact with them
– Aerosols may spread effectively over distances up to 50 m
– Common air space of stables may mean that all horses in that 
stable may be infected
• All animals within the primary perimeter
– Considered infected & contagious until outbreak is over
– Are prohibited from exiting the primary perimeter
– If the affected horse was moved to isolation, a primary 
biosecurity perimeter must be maintained around the barn from 
which the affected horse originated
• Biosecurity measures are implemented to prevent 
infectious agents leaving the area (people, fomites)
Suspected case of contagious
infectious respiratory disease
• Until proven otherwise, respond to ‘worst‐case’ 
scenario(s):
– Steptococcus equi (indirect transmission – fomites)
– Hendra virus (fruit bats)
– EI (direct transmission – aerosol; indirect – fomites)
• Prevent new introductions to the property
Suspected case of contagious
infectious respiratory disease
2. Implement/enforce the primary biosecurity perimeter
• Stop horse movement
– Affected horses should be confined to their stalls or 
dedicated isolation facility
– Clinically unaffected horses are confined within the primary 
perimeter & managed to minimise spread of the infectious 
agent
• Disease surveillance
– Record rectal temperatures twice daily
– Physical inspection for clinical signs
Suspected case of contagious
infectious respiratory disease
2. Implement/enforce the primary biosecurity perimeter
• Limit human movement
– Access is limited to essential personnel only: 
veterinarians/technicians/caretakers
– All personnel follow biosecurity protocols
– May require security personnel at perimeter access 
points
• Biosecurity protocols
Suspected case of contagious
infectious respiratory disease
3.1 Communication – event management
• Physical plant modification instructions
– Barriers – designation & establishment of physical perimeter
• Biosecurity guidelines
– Disinfection instructions
• During outbreak
• Before restocking facility with healthy horses
– Waste removal
– Vermin control
• Personnel management
– Requirements
– Instructions
– Notification of zoonotic risk (if present)
• Outbreak updates
Suspected case of contagious
infectious respiratory disease
3.2 Communication – veterinarians/workers
• Instructions – disease surveillance, testing & 
reporting
• Biosecurity guidelines
• Health requirements – entrance into or exit out 
of facility
• Outbreak updates
Suspected case of contagious
infectious respiratory disease
3.3 Communication – horse owners/workers
• Disease information for horsemen & owners
• Biosecurity guidelines
• Human exposure/zoonotic risk management
• Instructions for grooms
• Outbreak updates
• Requirements for equine entrance into/exit out 
of facility
Suspected case of contagious
infectious respiratory disease
4. Regulatory agencies
• Disease notification
– Veterinarians are aware of currently reportable diseases
– State & federal veterinarians are useful resources 
during outbreaks of non‐reportable infectious disease
• Outbreak updates
5. Media
• Dissemination of information to horse owning 
people & related industry workers/members
Suspected case of contagious
infectious respiratory disease
6. Related industries
• Provide information that assists in risk 
management & determination of risk aversion
– E.g. outbreak updates, biosecurity measures 
implemented
Lockout issue EHPC 2017
• November 2017
– One foal with suspected EHV1 infection
• Confirmed at post‐mortem
– Two consequent foals with similar symptoms form
same stud farm
– Delay is result collection !!!!
Suspected case of contagious
infectious respiratory disease
• Possible primary diagnoses for respiratory 
disease with herd health implications:
– Equine Herpes virus‐1 (EHV‐1)
– Equine Herpes virus‐4 (EHV‐4)
– Streptococcus equi equi (strangles)
– Hendra virus (emerging)
– Equine influenza (EI) (exotic)
– Equine viral arteritis (EVA) (exotic)
EHPC Standard Safe Operating 
Procedures
In the practice
Problematic
• 4 inpatient potentially but not surely
contaminated
• Hazard for any new in‐ or out‐patient
Precaution
• Preventive Lockout
• Isolate inpatients
• Collect samples and receive results in timely
manner to maintain or relieve quarantine
status
Suspected case of infectious 
respiratory disease
Establish biosecurity 
perimeter
Communicate plan Attempt diagnosis
Identify primary 
perimeter
Identify secondary 
perimeter
Increase disease 
surveillance
Regulate horse 
movement
Implement primary 
perimeter
Stop horse 
movement
Limit human 
movement
Biosecurity
Guidelines
Veterinarians
Horsemen
Regulatory agencies
media
Related industry 
affiliates
Event management
Sample collection
tests
Laboratory selection
Testing applies
Sample handling
Sample transport
No diagnosis
Maintain biosecurity
21‐28 days after last case
Expand differential 
diagnoses
Consult infectious 
disease expert
Diagnosis
Disease specific guidelines
Equine Herpes virus‐1
Influenza
Strep equi
Equine viral arteritisAAEP 2006
Main message
• Have or implement a biosecurity plan for your
facilities and taylored to your activities
• Your attending veterinarian is your best 
contact to get advise or relay to specialists
(EHPC)

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