This document discusses biomolecules. It begins by defining biomolecules as chemicals or molecules present in living organisms. The sum total of different types of biomolecules, compounds, and ions present in a cell is called the cellular pool. Biomolecules are compounds of carbon, so the chemistry of living organisms is organized around carbon. The major biomolecules in cells include inorganic molecules like minerals and gases, organic molecules like carbohydrates, lipids, proteins, and nucleic acids, and smaller molecules like amino acids and nucleotides.

1. BIOMOLECULES

2. Chemicals or molecules present in the living organisms are known
as Biomolecules
The sum total of different types of biomolecules, compounds and
ions present in a cell is called as cellular pool
Biomolecules are compounds of carbon.
Hence the chemistry of living organisms is organized around carbon
Carbon is the most versatile and the most predominant element of life.
ELEMENT Non living
(Earth crust)
Living Matter
Hydrogen 0.14 0.5
Carbon 0.03 18.5
Oxygen 46.6 65.0
Nitrogen Very less 3.3
Sulphur 0.03 0.3
Sodium 2.8 0.2
Calcium 3.6 1.5
Magnesium 2.1 0.1
Silicon 27.7 Very less

3. BIOMOLECULES
Inorganic Organic
Minerals
Gases
Water
Carbohydrates
Lipids
Amino acids
Proteins
Enzymes
Nucleotides
Nucleic acids
Vitamins

4. BIOMOLECULES
Minerals
Gases
Water
Sugars
Amino acids
nucleotides
Carbohydrates
Lipids
Proteins
Nucleic acids
Micro molecules
Small sized, low mol wt
Between 18 and 800 daltons
Found in the acid soluble pool
Macromolecules
Large sized, high mol wt
Above 10000 daltons
Found in the acid insoluble pool

5. Biomolecule Building block Major functions
Protein Amino acid Basic structure and function
of cell
DNA Deoxyribonucleotide Hereditary information
RNA Ribonucleotide Protein synthesis
Polysaccharide Monosaccharide Storage form of energy
Lipids Fatty acids & glycerol Storage form of energy to
meet long term demands
THE MAJOR COMPLEX BIOMOLECULES OF CELLS

6. NUCLEOTIDES

7. SUGAR
N2 BASES

9. dr.aarif
DNA

11. The strand backbones are closer
together on one side of the helix
than on the other. The major
groove occurs where the backbones
are far apart, the minor groove
occurs where they are close
together. The grooves twist around
the molecule on opposite sides.
Certain proteins bind to DNA to
alter its structure or to regulate
transcription (copying DNA to RNA)
or replication (copying DNA to
DNA). It is easier for these DNA
binding proteins to interact with the
bases (the internal parts of the DNA
molecule) on the major groove side
because the backbones are not in
the way.

12. m- RNA r- RNA t- RNA

13. CARBOHYDRATES

14. CARBOHYDRATES
Carbohydrates are the most abundant organic molecules in nature.
The term carbohydrate is derived from the French term
hydrate de carbone i.e. it is a hydrate of carbon or Cn(H2O)n
Carbohydrates are defined as organic substances having C, H & O
Wherein H and O are in the ratio 2:1 as found in H2O
FUNCTIONS OF CARBOHYDRATES
- Most abundant source of energy (4 cal/g)
- Precursors for many organic compounds (fats, amino acids)
- Present as glycoproteins and glycolipids in the cell
memebrane and functions such as cell growth and fertilization
- Present as structural components like cellulose in plants,
exoskeleton of some insects, cell wall of microorganisms
- Storage form of energy (glycogen) to meet the energy
demands of the body.

15. CARBOHYDRATES
MONOSACCHARIDES OLIGOSACCHARIDES POLYSACCHARIDES
Basic units of carbohydrates
Cannot be hydrolysed into
smaller units
They can be further
hydrolysed
Non crystalline, non soluble
in water, tasteless, on
hydrolysis gives mol of
monosaccharides
e.g. starch , cellulose
a. Based on the no. of
C-atoms
b. Based on the type of
functional group
a. Disaccharides
b. Trisachharides
c. T
etrasachharides

16. MONOSACCHARIDES
Based on the no of C-atoms
- Trioses (C3H6O3)
e.g. Glyceraldehyde,
Dihydroxyacetone
- Tetroses (C4H8O4)
e.g. Erythrose, Threose
- Pentoses (C5H10O5)
e.g. Ribulose, Xylose
Arabinose
(deoxyribose – C5H10O4)
- Hexoses (C6H12O6)
e.g. glucose, fructose
galactose, mannose
- Heptoses (C7H14O7)
e.g. sedoheptulose
glucoheptose
Based on the functional group
- Aldoses : the functional group is
Aldehyde –CHO
e.g. Glyceraldehyde, glucose
- Ketoses : the functional
group is ketone
( C = O)
e.g. Dihydroxyacetone,
fructose

17. DERIVATIVES OF MONOSACCHARIDES
1. Deoxy Sugars :
2. Amino Sugars :
3. Sugar Acid :
Deoxygenation of ribose produces deoxyribose,
which is a structural component of DNA
When 1 or more –OH groups of monosaccharides
are replaced by –NH2 (amino group) it forms an
amino sugar e.g. Glucosamine, which forms
chitin, fungal cellulose, hyaluronic acid.
Oxidation of –CHO or –OH group forms sugar
acids. Ascorbic acid is a sugar acid
4. Sugar alcohols : Reduction of aldoses or ketoses.
Glycerol and Mannitol.

18. OLIGOSACCHARIDES
They are formed by condensation of 2-9 monosaccharides
Depending upon the no. of monosachharide molecules they are :
a. Disaccharides (sucrose, lactose)
b. Trisaccharides (raffinose)
c. Tetrasaccharides (stachyose)
The smallest and the commonest oligosaccharides are Disaccharides

19. DISACCHARIDES
A disaccharide consists of 2 monosaccharide units (similar or dissimilar)
held together by a glycosidic bond
They are crystalline, water soluble and sweet to taste.
MALTOSE : - is also called as malt sugar.
- made up of 2 glucose molecules
LACTOSE : - is also called as milk sugar as it is found naturally in
milk
- made up of glucose and galactose
- souring of milk is due to conversion of lactose to
lactic acid
SUCROSE : -is also called as cane sugar. It is the sugar found in
sugar cane and sugar beet
- most abundant among naturally occuring sugars.
- Important source of Dietary carbohydrates
- made up of glucose and fructose

20. POLYSACCHARIDES
- Also called as GLYCANS
- Made up of repeating units of monsaccharides held by glycosidic
bonds
- During its formation a water molecule is released at each
condensation
This helps reduce the bulk making it almost insoluble
decreasing its effect on the water potential or osmotic potential of
the cell
- Unlike sugars they are not sweet.
- They are ideal as STORAGE AND AS STRUCTURAL COMPONENTS
- They are of 2 types Homoglycans and Heteroglycans.
HOMOGLYCANS
-Made up of only 1 type of
monosaccharide monomers
-For eg starch, glycogen,
cellulose
-Glucan (made up of glucose)
-Fructan(made up of fructose)
-Galactan (made up of galactose)
HETEROGLYCANS
-Made up condensation of2 or
more types of monosaccharides
- For eg Hyaluronic acid, agar,
Chitin, peptidoglycans etc

21. STORAGE
POLYSACCHARIDES
STARCH
1. Carbohydrate reserve of plants and the most important dietary source
for animals
2. High content of starch in cereals, roots, tubers, vegetables etc.
3. Homopolymer made up of GLUCOSE units. Also called as GLUCAN.
4. Starch = Amylose + Amylopectin (polysaccharide components)
GLYCOGEN
1. Carbohydrate reserve in animals. Hence referred as animal starch
2. High concentration in Liver, muscles and brain.
3. Also found in plants that do not have chlorophyll (yeast and fungi)
4. GLUCOSE is the repeating unit.
INULIN
1. Polymer of fructose i.e. fructosan
2. Found in Dahlia, bulbs, garlic, onion etc
3. Easily soluble in water
4. Inulin is not readily metabolised in the human body and is readily filtered
through the kidney. Hence used for testing kidney function (GFR)

22. STRUCTURAL
POLYSACCHARIDES
CHITIN
1. Second most abundant organic substance.
2. Complex carbohydrate of Heteropolysaccharide type.
3. Found in the exoskeletons of some invertebrates like insects and
crustaceans. Provides both strength and elasticity.
4. Becomes hard when impregnated with calcium carbonate.
CELLULOSE
1. Occurs exclusively in plants and is the most abundant organic
substance in plant kingdom.
2. Predominant constituent of plant cell wall.
3. It is totally absent in animals.

23. PROTEINS

24. PROTEINS
 Most abundant organic molecules of the living system.
 They form about 50% of the dry weight of the cell.
 They are most important for the architecture and functioning
of the cell.
Proteins are polymers of amino acids
 Proteins on complete hydrolysis yields Amino Acids
 There are 20 standard amino acids which are repeatedly found
in the structure of proteins – animal, plant or microbial.
 Collagen is the most abundant animal protein and Rubisco is
the most abundant plant protein
 Protein Synthesis is controlled by DNA.

25. AMINO ACIDS
 Amino acids are group of organic compounds
having 2 functional groups (-NH2) and (-COOH)
 (-NH2) group is basic whereas (-COOH) is acidic
 R- can be H in glycine, CH3 in alanine, Hydroxymethyl in serine
in others it can be hydrocarbon chain or a cyclic group
 All amino acids contain C, H, O and N but some of them additionally
contain S
 Physical and chemical properties of amino acids are due to
amino, carboxyl and R functional groups
 Amino acids are differentiated into 7 groups

26. No. Nature Amino acids
1. NEUTRAL : Amino acids with 1 amino and 1
carboxyl group
Glycine (Gly), Alanine (Ala),
Valine (Val), Leucine (Leu),
Isoleucine (Ile)
2. ACIDIC : 1 extra carboxyl group Aspartic acid (Asp),
Asparagine (Asn), Glutamic
acid (Glu), Glutamine (Gln)
3. BASIC : 1 extra amino group Arginine (Arg), Lysine (Lys)
4. S – CONTAINING : Amino acids have sulphur Cysteine (Cys), Methionine
(Met)
5. ALCOHOLIC : Amino acids having –OH group Serine (Ser), Threonine
(Thr), Tyrosine (Tyr)
6. AROMATIC : Amino acids having cyclic
structure
Phenylalanine (Phe),
Tryptophan (try)
7. HETEROCYCLIC : amino acids having N in
ring structure
Histidine (His), Proline (Pro)

27. Aspartic acid (acidic)
ALANINE
(NEUTRAL) Lysine (basic)
Serine (alcoholic) Cysteine (suphur) Phenylalanine (aromatic)

28. PEPTIDE
FORMATION
 Amino acids are linked serially by peptide
bonds (-CONH-) formed between the
(-NH2) of one amino acid and the
(-COOH) of adjacent amino acid
 Chain having 2 amino acids linked by a
peptide bond is called as a DIPEPTIDE
 The sequence of amino acids present in
a polypeptide is specific for a particular
protein.

29. STRUCTURE OF
PROTEIN
4 basic structural levels are assigned to
proteins – primary, secondary, tertiary
and quaternary
PRIMARY
The primary structure refers to the number
and linear sequence of amino acids in the
polypeptide chain and the location of the
disulphide bridges
The primary structure is responsible for the
function of the protein.
The N-terminal amino acid is written on the
left side whereas the C- terminal amino acid
is written on the right side

30. SECONDARY
The folding of the linear chain into a specific coiled
structure is called as secondary structure.
3 types : α- helix, β- pleated sheet and collagen
helix
α- helix β- pleated sheet Collagen helix

31. TERTIARY
The helical polypeptide may fold upon itself and assume a complex but
specific form – spherical, rod like or something in between.
These geometrical shapes are known as tertiary (30) structure
QUATERNARY
Proteins are said to be quaternary in structure
If they have 2 or more polypeptide chains
Haemoglobin is an excellent example

33. PROTEINS
Function
Chemical nature
and solubility
Nutritional
importance

34. Functional
classification
Structural proteins e.g. keratin, collagen
Enzymatic proteins e.g. pepsin
Transport proteins e.g. Haemoglobin
Hormonal proteins e.g. Insulin, Growth hormone
Contractile proteins e.g. Actin, myosin
Storage proteins e.g. Ovalbumin
Genetic proteins e.g. Nucleoproteins
Defence proteins e.g. Imunoglobulins
Receptor proteins e.g. for hormones and viruses

35. CLASSIFICATION
BASED ON CHEMICAL
NATURE AND
SOLUBILITY
1. Simple proteins : They are composed only of amino acid
residues
2. Conjugated proteins : Along with amino acids , there is a
non-protein prosthetic group.
3. Derived proteins : They are denatured or degraded products
of the above two

36. PROTEI
NS
Simple
Conjugated
Derived
Nucleoproteins
Glycoproteins
Mucoproteins
Lipoproteins
Phosphoproteins
Chromoproteins
Metalloproteins
Globular Fibrous
Albumin
Globulin
Histones
Collagen
Elastin
Keratin
Primary Secondary
Coagulated
proteins
Proteans
Metaproteins
Proteoses
protones
Peptides

37. LIPIDS

38. Lipids are the chief concentrated storage form of energy forming
about 3.5% of the cell content.
Lipids are organic substances relatively insoluble in water but
soluble in organic solvents (alcohol, ether)
Functions :
1. They are the concentrated fuel reserve of the body.
2. Lipids are constituents of membrane structure and regulate the
membrane permeability.
3. They serve as source of fat soluble vitamins
4. Lipids are important cellular metabolic regulators
5. Lipds protect the internal organs and serve as insulating materials

39. LIPIDS
Simple
Complex
Derived
Phospholipids
Glycolipids
Lipoproteins
Fats & Oils Waxes
Steroids
T
erpenes

40. SIMPLE LIPIDS
They are esters of fatty acids with alcohol. They are of 2 types :
1. Neutral or true fats : Esters of fatty acids with glycerol
2. Waxes : Esters of fatty acids with alcohol other than glycerol.
Neutral / True
fats
True fats are made up of C, H, & O but O is less
A fat molecule is made up of 2 components :
a) GLYCEROL
b) FATTY ACIDS (1-3 mol, of same or diff long chained)

41. GLYCER
OL
A glycerol mol has 3 carbons each bearing a
–OH group
Fatty acid
A fatty acid mol is an unbranched chain of C-
atoms.
It has a –COOH group at one end and a H
bonded to almost all the C-atoms
Fatty acids may be saturated or unsaturated

42. WAXES
Lipids which are long chain saturated fatty acids and a long chain
Saturated alcohol of high mol wt other than glycerol
Example :
1. Bees wax : secretion of abdominal glands of worker honey bees
2. Lanolin or wool fat : Secretion of cutaneous glands and obtained
from the wool of sheep
3. Sebum : secretion of sebaceous glands of skin
4. Cerumen : soft and brownish waxy secretion of the glands in the
external auditory canal. Also called as Earwax
5. Plant wax : Coating formed on the plant organs to prevent
transpiration
6. Paraffin wax : A translucent waxy substance obtained from
petroleum

43. COMPLEX
LIPIDS
They are derivatives of simple lipids having
additional group like phosphate, N2-base,
Protein etc. They are further divided into
Phospholipids, Glycolipids, Lipoproteins.
Phospholipid They are made up of a molecule of glycerol
Or other alcohol having
1. A phos group at 1 of its –OH groups
2. 2 fatty acid molecules at other 2 –OH
groups
3. A nitrogen containing base attatched to
phos group
A phospholipid molecule has a
hydrophobic tail (fatty acids) and a
hydrophilic head(phos group)

44. GLYCOLIPID
They are components of cell
membranes, particularly myelin
sheath and chloroplast membranes
CEREBROSIDE are the most
simplest form of glycolipids

45. LIPOPROTEIN
They contain lipids and proteins in their molecules.
They are main constituent of membranes.
They are found in milk and Egg yolk.
Lipids are transported in blood and lymph as lipoproteins.
5 types of lipoproteins :
1. chylomicrons
2. VLDL
3. LDL
4. HDL
5. Free fatty acid albumin complex

46. DERIVED
LIPIDS
• They are made up of 4
fused carbon rings
• Cholesterol, Vit D,
testosterone,
adrenocortical
hormones.
• The most common steroids
are
• STEROLS.
They are derivatives obtained on the
hydrolysis of the simple and complex lipids.
e.g. steroids, terpenes and prostaglandins
Steroid The steroids do not contain fatty acids
but are included in lipids as they have
fat-like properties.
Common sterols are Cholesterol and
ergosterol

47. TERPENES
Terpenes are a major component of essential oils produced by
plants. They give fragrance to the plant parts.
Vitamins A, E and K contain a terpenoid called phytol
Carotenoid pigment is precursor for Vitamin A
Lycopene, a pigment present in tomatoes is a terpenoid
Gibberellins, the plant hormone is also a terpene

48. Group of Enzymes Reactions catalysed Examples
1. Oxidoreductases Transfer of O2 or H2 atoms
or electrons from one
substrate to another
Dehydrogenase
Oxidases
2.Transferases Transfer of a specific group
from one substrate to
another
Transaminase
3.Hydrolases Hydrolysis of a substrate Digestive enzymes
4. Isomerases Change of the molecular
form of the substrate
Phospho Hexo
isomerase
5.Lyases Non hydrolytic removal or
addition of a group to a
substrate
Decarboxylase
Aldolase
6. Ligases Joining of 2 molecules by
formation of new bonds
Citric acid
synthetase