BIOL302L Poster(1)
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This experiment aimed to determine the relative size of the arabinose operon in E. coli MC4100 using MuD1 lysogeny. MuD1 contains the bla gene conferring ampicillin resistance. The frequency of ampicillin resistance (AmpR) was compared to the frequency of arabinose resistance (AraR) after infecting E. coli MC4100 with MuD1. The ratio of AraR to AmpR lysogens was 1:161, indicating the arabinose operon accounts for 0.62% of the genome or approximately 644 total genes in E. coli MC4100. However, the authors express doubt in these conclusions and state the experiment needs to be repeated to ensure
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Safi et al.ng.2743
This document summarizes a study investigating the evolution of high-level resistance to the tuberculosis drug ethambutol in Mycobacterium tuberculosis. The key findings are:
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Plasmids are a mechanism of gene diversity. In order to stably retain the plasmid, there needs to
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Lab: Differential Expression Differential gene expression provides the ability for a cell or
organism to respond to a constantly changing external environment. The specific constellation of
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context. Thus, protein production is carefully regulated by multiple mechanisms that modulate
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resistance), 3. Additional gene for ease of visual screening 4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
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Melanoma and Parkinson disease & Link between them.
The B30. 2 domain of pyrin, the familial mediterranean fever protein, interac...
The B30. 2 domain of pyrin, the familial mediterranean fever protein, interac...
Similar to BIOL302L Poster(1)
Lab Differential Expression Differential gene expression provides th.pdf
Lab: Differential Expression Differential gene expression provides the ability for a cell or
organism to respond to a constantly changing external environment. The specific constellation of
proteins required for optimal function and growth varies with cellular age and environmental
context. Thus, protein production is carefully regulated by multiple mechanisms that modulate
both transcriptional and translational pathways. Control of transcription initiation by RNA
polymerase is a predominant mechanism for regulating expression of specific proteins,
presumably because it provides maximal conservation of energy for the cell. We can often
observe the consequence of differential transcription due to the presence or absence of particular
proteins or the growth in particular environments. Control can also occur at translation; the
mRNA is synthesized, but only in certain circumstances is it translated. Control can also occur at
the level of protein function; the protein is inactive, or activity is not observed due to the lack of
the substrate. In this lab we will observe differential expression of two different genes encoded
on plasmids. We will analyze transcriptional activity, translational activity, and protein function.
Plasmids are extra-chromosomal DNA. Bacteria often have plasmids and will replicate the
plasmid and pass it to daughter cells (vertical transmission) and to neighboring cells (horizontal).
Plasmids are a mechanism of gene diversity. In order to stably retain the plasmid, there needs to
be some type of metabolic reason for the bacteria to maintain the plasmid. In other words, the
plasmid confers an advantage. Plasmids contain: 1. Ori: the plasmid may present is low or high
copy number. 2. Lab generated plasmids typically also contain a selectable marker (antibiotic
resistance), 3. Additional gene for ease of visual screening 4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
recombinant plasmids. The transformed cells containing the plasmid with the gene of interest ca.
Lab Differential Expression Differential gene expression provides .pdf
Lab: Differential Expression Differential gene expression provides the ability for a cell or
organism to respond to a constantly changing external environment. The specific constellation of
proteins required for optimal function and growth varies with cellular age and environmental
context. Thus, protein production is carefully regulated by multiple mechanisms that modulate
both transcriptional and translational pathways. Control of transcription initiation by RNA
polymerase is a predominant mechanism for regulating expression of specific proteins,
presumably because it provides maximal conservation of energy for the cell. We can often
observe the consequence of differential transcription due to the presence or absence of particular
proteins or the growth in particular environments. Control can also occur at translation; the
mRNA is synthesized, but only in certain circumstances is it translated. Control can also occur at
the level of protein function; the protein is inactive, or activity is not observed due to the lack of
the substrate. In this lab we will observe differential expression of two different genes encoded
on plasmids. We will analyze transcriptional activity, translational activity, and protein function.
Plasmids are extra-chromosomal DNA. Bacteria often have plasmids and will replicate the
plasmid and pass it to daughter cells (vertical transmission) and to neighboring cells (horizontal).
Plasmids are a mechanism of gene diversity. In order to stably retain the plasmid, there needs to
be some type of metabolic reason for the bacteria to maintain the plasmid. In other words, the
plasmid confers an advantage. Plasmids contain: 1. Ori: the plasmid may present is low or high
copy number. 2. Lab generated plasmids typically also contain a selectable marker (antibiotic
resistance), 3. Additional gene for ease of visual screening 4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
recombinant plasmids. The transformed cells containing the plasmid with the gene of interest ca.
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE B.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE
BACKGROUND CONTEXT Lab: Differential Expression Differential gene expression provides
the ability for a cell or organism to respond to a constantly changing external environment. The
specific constellation of proteins required for optimal function and growth varies with cellular
age and environmental context. Thus, protein production is carefully regulated by multiple
mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
expression of specific proteins, presumably because it provides maximal conservation of energy
for the cell. We can often observe the consequence of differential transcription due to the
presence or absence of particular proteins or the growth in particular environments. Control can
also occur at translation; the mRNA is synthesized, but only in certain circumstances is it
translated. Control can also occur at the level of protein function; the protein is inactive, or
activity is not observed due to the lack of the substrate. In this lab we will observe differential
expression of two different genes encoded on plasmids. We will analyze transcriptional activity,
translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
have plasmids and will replicate the plasmid and pass it to daughter cells (vertical transmission)
and to neighboring cells (horizontal). Plasmids are a mechanism of gene diversity. In order to
stably retain the plasmid, there needs to be some type of metabolic reason for the bacteria to
maintain the plasmid. In other words, the plasmid confers an advantage. Plasmids contain: 1. Ori:
the plasmid may present is low or high copy number. 2. Lab generated plasmids typically also
contain a selectable marker (antibiotic resistance), 3. Additional gene for ease of visual screening
4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
recombinant.
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE BAC.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE
BACKGROUND CONTEXT Lab: Differential Expression Differential gene expression provides
the ability for a cell or organism to respond to a constantly changing external environment. The
specific constellation of proteins required for optimal function and growth varies with cellular
age and environmental context. Thus, protein production is carefully regulated by multiple
mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
expression of specific proteins, presumably because it provides maximal conservation of energy
for the cell. We can often observe the consequence of differential transcription due to the
presence or absence of particular proteins or the growth in particular environments. Control can
also occur at translation; the mRNA is synthesized, but only in certain circumstances is it
translated. Control can also occur at the level of protein function; the protein is inactive, or
activity is not observed due to the lack of the substrate. In this lab we will observe differential
expression of two different genes encoded on plasmids. We will analyze transcriptional activity,
translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
have plasmids and will replicate the plasmid and pass it to daughter cells (vertical transmission)
and to neighboring cells (horizontal). Plasmids are a mechanism of gene diversity. In order to
stably retain the plasmid, there needs to be some type of metabolic reason for the bacteria to
maintain the plasmid. In other words, the plasmid confers an advantage. Plasmids contain: 1. Ori:
the plasmid may present is low or high copy number. 2. Lab generated plasmids typically also
contain a selectable marker (antibiotic resistance), 3. Additional gene for ease of visual screening
4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
recombinant.
DNA Vaccine + Nanoparticles
The document discusses developing a DNA vaccine for fish using chitosan nanoparticles to deliver plasmid DNA encoding the OMP38 gene of Vibrio anguillarum. Key points:
- Chitosan nanoparticles were developed to deliver the pVAOMP38 plasmid and protect it from nuclease degradation. Studies showed the nanoparticles maintained plasmid integrity.
- The pVAOMP38 plasmid was transfected into seabass kidney cells in vitro and shown to express.
- Fish were vaccinated by feeding with chitosan-pVAOMP38 nanoparticles, chitosan-empty vector, or chitosan alone. The fish were later challenged with V. anguillarum to evaluate vaccine efficiency.
The Role Of Mir 125A And Mir 223
The document discusses the scientific discovery of selective breeding. Selective breeding involves breeding organisms within the same species to increase the frequency of desirable existing traits. It has been practiced for around 10,000 years to benefit humans in agriculture, farming, and medicine. Two main methods are selective breeding and transgenesis, which involves transferring genes between organisms to create new traits. Selective breeding techniques include marker assisted selection and original inbreeding/line breeding.
P 53 Tumour Biology
Mdm2 oncoprotein. Cell Mol Life Sci 55:96–107
1) Researchers identified a novel oncogene, ribosomal protein L10a (RPL10a), using a cDNA library from Daudi cells to transform p53 knockout mouse embryonic fibroblast cells.
2) Western blot analysis of tumors from myc mice showed different levels of p53 and ARF expression, indicating deregulation of the myc-ARF-p53 pathway.
3) Sequencing of DNA from foci identified RPL10a as a potential oncogene, and its interaction with Mdm2 may imitate effects of myc overexpression, contributing to tumor progression in the absence of p53
Application1
The document discusses gene cloning, expression, and functional study. It describes different types of vectors used for cloning genes, including cloning vectors, expression vectors, and integration vectors. It provides details on various cloning vectors such as plasmid vectors, bacteriophage vectors, cosmids, BACs, and eukaryotic vectors. It also describes expression vectors and components required for gene expression. Finally, it discusses bacteriophage vectors, cosmids, YAC vectors, and BAC vectors which are used to clone large DNA fragments from eukaryotes.
Crop genome editing using CRISPR
Genome editing uses tools like CRISPR/Cas9 to make precise, targeted changes to DNA in crop plants. The document discusses using CRISPR to edit the SlMlo1 gene in tomato, conferring resistance to powdery mildew. Researchers generated CRISPR/Cas9 plasmids targeting SlMlo1, transformed tomato plants, and identified lines with 48 bp deletions in the gene. Resistant T1 plants lacking the transgene were obtained, named the Tomelo variety. Genome editing provides a faster way to introduce disease resistance than conventional breeding.
Petrova_Poster_06 19 2012
This study investigated how changes in cadherin structure at the cell surface regulate adhesive activity. The researchers found:
1) Activating monoclonal antibodies recognize conformational epitopes near calcium binding sites between cadherin domains, inducing adhesion.
2) Antibody activation induces dephosphorylation of specific residues in p120-catenin's N-terminal domain, mediating adhesion.
3) Phosphorylation-deficient and phosphomimetic p120 mutants in cells had opposite effects on adhesion, indicating p120 phosphorylation directly controls cadherin adhesion.
Screening and selection of recombinants
The document discusses various methods for screening and selecting recombinant cells. Direct selection methods include antibiotic resistance screening and blue-white color screening. Indirect selection methods include screening by nucleic acid hybridization, colony hybridization, immunological assays, and detecting protein/enzyme activity. These screening methods allow identification of recombinant cells that contain the gene of interest from a mixture of transformed cells.
P bluescript
pBluescript is an example of a combination between plasmids and phages (phagemids).
Phagemids represent a hybrid type of class of vectors that serve to produce single-stranded DNA.
Anatomy of a gene
1. Fred Griffith discovered that a substance present in the virulent S strain of Streptococcus pneumoniae could permanently transform the nonlethal R strain into the deadly S strain.
2. Avery, MacLeod, and McCarty identified this "transforming principle" as DNA, providing the first evidence that DNA serves as the genetic material.
3. Hershey and Chase used radioactively labeled proteins and DNA from T2 viruses to infect E. coli cells. They found that most of the radioactively labeled DNA entered the bacterial cells while most of the labeled proteins remained outside, demonstrating that the genetic material of viruses is DNA rather than protein.
ATAC-seq protocol--Creative Biogene
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to study chromatin accessibility. The key part of the ATAC-seq procedure is the action of the transposase Tn5 on the genomic DNA of the sample.
Methods of screening
This document discusses various methods for screening recombinant clones, including:
1. Blue-white screening using X-gal to detect the presence or absence of insertions disrupting beta-galactosidase activity.
2. Insertional inactivation screening by inserting DNA fragments to disrupt antibiotic resistance or repressor genes.
3. Antibiotic sensitivity screening to detect circularized recombinant plasmids capable of conferring antibiotic resistance.
4. Auxotrophic yeast strain screening using yeast vectors containing selectable marker genes to complement host mutations.
5. Reporter gene assays using enzymes like luciferase, GFP, etc. fused to promoters to identify positive recombinant clones.
Analysis of recombinants.pptx
1) There are several methods for selecting and screening recombinant transformants, including blue-white screening using X-gal, insertional inactivation of antibiotic resistance genes, and using reporter genes like luciferase or GFP.
2) Gene transfer in animals can be done using viral vectors like retroviruses which incorporate the transgene into their genome, or using non-viral methods like microinjection, gene guns, electroporation, and ultrasound to deliver naked DNA into cells.
3) Stable transfection integrates transferred DNA into the host chromosome for permanent expression, while transient transfection expresses DNA temporarily without integration.
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
Current Attempt in Progress Sexpili are to conjugation as is/are to transduction. bacteriophages
plasmids genes the host chromosome
The anticodon of tRNA interacts with the
codon on mRNA. amino acid. backbone on rRNA. ATP for reaction energetics
A microbiologist designs an experiment to transfer a gene from a pathogenic bacterium into a
laboratory strain of Escherichia colit discover whether the gene confers virulence. One method
for transferring the gene into E. coli would be to use a ligase reaction. restriction digestion.
polymerase chain reaction. cloning vector. sequencing reaction.
x Your answer is incorrect. What sites do eukaryotes have for gene regulation that are not found
in prokaryotes? Select all that apply. Nucleus and cytoplasm Operons Poly(A) talis Ribosomes
Histones
When E. coli in added to a medium with lactose and no glucose, what happens to the repressor
system of the lac operon? 1. The lac repressor is inactivated 2. Transcription of the lac operon
genes into a single mRNA 3. -galactosidase and lac permease are synthesized 4. Lactose is
rapidly metabolized by enzymes 1, 2,3 and 4 2, 3 and 4 1,3 and 4 1,2 and 3
Which of the following are found as part of an operon? Select all that apply. Structural genes
operator promoter intron ribosome
DNA (Select all that apply) is a double stranded molecule. is used as a template to synthesize
proteins. contains the hereditary information of a cell. contains all bases A, T, C and G..
Western Blotting Of Camkii Β And T 287
1. Tomato production is affected by various bacterial, fungal and viral diseases which can cause considerable yield losses.
2. One of the most devastating diseases is tomato leaf curl disease (ToLCD), caused by geminiviruses, which is increasing worldwide and poses a major constraint to tomato production in India.
3. ToLCD causes serious yield losses according to studies from the 1940s and more recently. Effective management strategies are needed to control this and other diseases threatening tomato production.
Yeast Artificial Chromosome (YAC)
Yeast artificial chromosomes (YACs) are engineered DNA molecules that can clone large segments of DNA from organisms in yeast cells. YACs are constructed by inserting DNA fragments between the two arms of a YAC vector in yeast cells through homologous recombination. The YAC vectors contain elements necessary for the vector to behave like a yeast chromosome, including an origin of replication, centromere, and telomeres. YACs allow cloning and stable maintenance of eukaryotic DNA segments up to 1 million base pairs in length, which has enabled genome mapping, sequencing, and analysis of gene expression regulation.
PLASMID BORNE GENE EXPRESSION IN PROKARYOTES.pptx
Plasmids are small, circular pieces of DNA that can be found in prokaryotic cells. They can be naturally occurring or artificially created, and they can carry genes that are not found in the host cell's genome. Plasmids can be used to express genes in prokaryotes in a number of ways.
One way to express a plasmid-borne gene is to use a promoter. A promoter is a sequence of DNA that binds to RNA polymerase, the enzyme that initiates transcription. When RNA polymerase binds to a promoter, it begins to transcribe the gene, which produces a messenger RNA (mRNA) molecule. The mRNA is then translated into a protein by ribosomes.
Another way to express a plasmid-borne gene is to use a transcription factor. A transcription factor is a protein that binds to a specific sequence of DNA and regulates the transcription of genes. When a transcription factor binds to a DNA sequence, it can either increase or decrease the rate of transcription of the gene.
Plasmid-borne gene expression can be used for a variety of purposes, including:
Producing proteins for research or industrial purposes
Engineering bacteria to produce new products or to perform new functions
Developing vaccines or other medical treatments
Plasmid-borne gene expression is a powerful tool that can be used to manipulate the genetic makeup of prokaryotic cells. This technology has the potential to revolutionize the way we produce food, develop new medicines, and protect the environment.
Here are some additional points about plasmid borne gene expression in prokaryotes:
The copy number of a plasmid can affect the level of gene expression.
The host cell's environment can also affect the level of gene expression.
Plasmids can be used to transfer genes between different prokaryotic cells.
Plasmids can be used to create new strains of bacteria with desired properties.
Similar to BIOL302L Poster(1) (20)
Lab Differential Expression Differential gene expression provides th.pdf
Lab Differential Expression Differential gene expression provides th.pdf
Lab Differential Expression Differential gene expression provides .pdf
Lab Differential Expression Differential gene expression provides .pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE B.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE B.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE BAC.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE BAC.pdf
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
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Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
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Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
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四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
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◇申请留学生创业基金
留信网认证的作用:
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2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
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Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
3D Hybrid PIC simulation of the plasma expansion (ISSS-14)
3D Particle-In-Cell (PIC) algorithm,
Plasma expansion in the dipole magnetic field.
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BIOL302L Poster(1)
- 1. Expression of araBAD Operon and Ampicillin Resistance in E. coli MC4100 Catherine Bane, Mintong Nan Department of Biological Sciences, University of Maryland Baltimore County Abstract This research aims to determine the relative size of the arabinose operon in comparison to the entire MC4100 genome by operon fusion methods using φMuD1. MuD1, a derivative of φMu, has been modified by the addition of the lac structural genes and the β-lactamase (bla) gene, and by the removal of the lytic genes. In this experiment, a lysate containing MuD1 was prepared by heat-induction of strain MAL103, a dilysogen carrying MuCts and MuD1. MC4100 cells were infected with the MuD1 lysate and plated on MacConkey plates with either Lactose or Arabinose in the presence of ampicillin. The bla gene codes for ampicillin resistance, allowing for the selection of lysogens on the lactose + ampicillin MacConkey plates. MC4100 cells are araD-, and thus die in the presence of arabinose. The arabinose + ampicillin MacConkey plates allow for the selection of arabinose resistant lysogens. By comparing the frequency of AmpR lysogens to the frequency of AraR lysogens, we conclude that the arabinose operon accounts for 0.62% of the MC4100 genome. Background The derivative of ΦMu, ΦMuD1has the ability to insert anywhere within the genome of its host, including inside of genes. The bla gene codes for enzymes that are capable of lysing the β-lactam ring found in many antibiotics, like penicillins. The addition of the bla gene to MuD1 provides antibiotic resistance. If the phage successfully inserts into the host cell DNA, the resulting lysogen will be resistant to the antibiotic. The arabinose operon contains genes encoding enzymes needed for the breakdown of arabinose. It contains a regulator, araC, and three structural genes, araB, araA, and araD. araC exerts both negative and posititve regulation by repressing transcription of itself and activating the structural genes, respectively.. Data and Results . Selection of MuD1 Lysogens: Selection of the AraR Lysogens: Dilution of Lysate Number of Plaques 10-5 TMTC 10-6 52 10-7 4 Titration of the Lysate: 0 20 40 60 80 100 120 140 NumberofColonies The Number of Ampicillin Resistant Colonies Compared to the Number of Arabinose Resistant Colonies AraR Lysogens AmpR Lysogens Lysate Titer= 5.2 X 108 pfu/mL Frequency of lysogeny to ampicillin resistance: 7.44 X 10-4 AmpR lysogens/ pfu Frequency of lysogeny to arabinose resistance: 4.62 X 10-6 AraR lysogens/ pfu Conclusions The frequency of lysogeny to AmpR is much higher than the frequency of lysogeny to AraR. • The calculated ratio is 161:1, meaning that the arabinose operon covers 0.62% of the MC4100 genome. • Since the arabinose operon is comprised of four genes, we can estimate that there are approximately 644 genes in the entire MC4100 genome. Our patched AraR colonies tell us the exact location of the MuD1 insertion: • I. • III. Using the percentage of red colonies from the selection of MuD1 lysogens, we found that approximately 10.8% of the MC4100 genome is transcribed on the MacConkey Medium. This number was calculated by doubling the red colony percentage since MuD1 could be orientated either towards or away from the promoter. Discussion It seems unlikely to us that the entire E. coli MC4100 genome contains only 644 genes, and that 4 of the 644 are involved in the metabolism of arabinose. The Mu genome contains 55 genes, it is 8.5% the size of the MC4100 genome. It is obvious to us that this experiment needs to be repeated many times to ensure accuracy in the data and its interpretations. Ratio of AraR lysogens/pfu: AmpR lysogens/pfu = 1: 161 # of Patches Showing each Pattern Color of Patch Arabinose MacConkey Lactose MacConkey Arabinose + Lactose MacConkey I.4 White White White II. 0 White White Red III. 1 White Red Red LacZ LacZ LacZ Patched AraR Colonies on Three Different Media: Acknowledgements and References We thank Dr. Steven Caruso for his laboratory assistance and expertise. Caruso, S.M., et al. 2014. Genetics Lab Manual: BIOL 302L Spring 2014. University of Maryland, Baltimore County. pp. 55-59. Morgan, Gregory J., Graham F. Hatfull, Sherwood Casjens, and Roger W. Hendrix. "Bacteriophage Mu Genome Sequence: Analysis and Comparison with Mu-like Prophages in Haemophilus, Neisseria and Deinococcus." Journal of Molecular Biology 317.3 (2002): 337-59.