Dolapo Akindele presented a seminar on using Lactobacillus vini as a fermentation organism for biofuel (bioethanol) production. The presentation covered how biotechnology can improve bioethanol production, described bioethanol and L. vini, and explained the fermentation process. It discussed that bioethanol is made through fermenting plants like corn, produced through a process where sugar is converted to ethanol and CO2 by yeast and L. vini, and then distilled and purified to high purity bioethanol. The presentation concluded that bioethanol is a renewable fuel that can serve as an alternative fuel for Nigeria.

1. A SEMINAR PRESENTATION
ON
LACTOBACILLUS VINI AS FERMENTATION
ORGANISM IN PRODUCTION OF BIO-FUEL (BIO-
ETHANOL)
BY
AKINYELE, DOLAPO DIMEJI
MATRIC NO: 140408016
DEPARTMENT: BIOLOGICAL SCIENCES
PROGRAMME: MICROBIOLOGY
MARCH, 2018

2. Introduction
Application of biotechnology to Improve
bio-ethanol production
Type of Bio-fuel
Bio-ethanol
Biology and source of Lactobacillus vini
Fermentation process involved in Bio-ethanol
Production
Conclusion
References
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3. BIOTECHNOLOGY
Use of living systems and organisms to develop or
make products, or any technological application that
uses biological systems, living organisms, make or
modify products or processes for specific use
(Springham et al. 1999).
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4. 3
BIO-FUEL
• Fuel that is produced through contemporary biological
processes (agriculture and anaerobic digestion)
• Fuel produced by geological processes (those involved in
the formation of fossil fuels, coal and petroleum, from
prehistoric biological matter.

5. Applications of Biotechnology to Improve Bio-ethanol
Production.
Enhanced or engineered microorganisms for fermentation
of ethanol, butanol, other fuels.
Engineered microorganisms or plants to manufacture
enzymes used in fuel production.
Improved algal strains for bio-ethanol production.
Selected or engineered plant species with favourable
traits for use as improved bio-ethanol feedstocks.
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6. 5
Figure 1: classification of bio-fuel
Source: www.omicsonline.org
CLASSIFICATION OF BIO-FUEL

7.  Form of renewable energy that can be produced from
agricultural feedstocks.
 Made from very common crops such as hemp, wheat,
sugarcane, potato, cassava and corn.
 Obtained from the conversion of carbon based feedstock.
 Fermentation.
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8.  ADVANTAGES
 Environmental Impact
 Spills and Surface Contamination
 Sulphur and Atmospheric Contamination
 Energy Independence
 DISADVANTAGES
 Regional Suitability
 Food Security
 Land Use Changes
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9.  Gram-positive, facultative anaerobic, rod shaped and non-spore-
forming bacteria.
 Lactic acid bacteria group (i.e they convert sugar to lactic acid).
 Constitute a significant component of the micro-biota at a number of
body sites, such as the digestive system, urinary system, and genital
system.
 Found as a normal micro-biota in the vaginal and gut of human
 Helps to maintain human well being and treat diarrhea, vaginal
infections and skin disorder such as enzema. 8

10. Bio-ethanol is manufactured by fermentation of plants in
the following steps;
Step 1: Fermentation of the sugar using yeast and L.
vini, whereby the sugar is converted to ethanol and CO2
Step 2: Distillation (separate the alcohol from the other
constituents of the mash).
Step 3: Ratification, i.e cleaning of the ethanol produced
by distillation which result in bio-ethanol with and
extremely high purity of 99.7% vol.
Step 4: dehydration of the bio-ethanol (Lin Y, et al
2006).
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11. 10
Figure 2: Bio-ethanol Production Process
Source: Large. stanford.edu
BIO-ETHANOL
PRODUCTION PROCESS

12.  Bio-ethanol is a natural product of
agricultural crops, which made it easy
to get, produce, renewable fuel, and also
serve as cooking and automobile power
source.
 The production and fermentation is a
simple process which can be carried out
in any local refinery.
 It is recommended that Nigeria adopts
this fuel as an alternative to fossil fuel.
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13. “Bio-fuel second generation Biofuel”.www.Biofuel.org.uk
Chisti, Y. (2007). Biodiesel from micro-algae. Bio-ethanol
Advances.25(3), 294-306.
Lin, Y., and Tanaka, S. (2006). Ethanol Fermentation from
Biomass Resources: Current State and Prospects.
Applied Microbiology Biotechnology.69, 627-642.
Springham, D. Moses, V. Cape, R. E. (1999).
Biotechnology; the science and the business. CRC
press. P.I. ISBN 978-90-5702-407-8.
www.large.stanford.edu
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