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BIOCOLORANTS
A potential food additive
Submitted To : Submitted By:
Mrs. Ritu Sindhu Sumit
Roll No.
( Food Engg.)M.Sc. (Foo.)
2/35
 Biocolor are those coloring agent which are
obtained from living source.They are
generally extracted from
fruits,vegetables,roots and micro organism.
Examples-
Annatto,Anthocyanins,Beetroots,Chlorophyll
,Carotenoids,Curcumine etc.
Biocolorant
3/35
 These are the organic colorants that are derived
from natural edible sources using recognized
food prepration methods.
 Ex. Curcumin, Bixin, Anthocyanins, chlorophyll
etc.
Natural Colour
6/35
 These are produced during cooking and
processing and thus may not be of any
direct importance in foods. For ex. As
produced during sugar caramelization ,
baking etc.
Browning Colour
 Food additive colours are based on
anthocyanins derived from sources such as
red grapes or beet but the first additive colour
were synthetic dyes which were extensively
used as food colorants.
Additives
 By biotechnological approach
 By microorganism
 By using certain enzymes
Production of Bio colour
 Genetic modification for pigment
production.
 Improving the traditional methods for
extraction of pigments
 Microbial production of pigments
 In vivo pigment production by plant tissue
culture
Biotechnology – Natural Colour
Production
 For the commercial extraction of astaxanthin
from Phaffia rhodozyma, extracellular
enzymes produced by Bacillus circulars are
used.
 These enzymes digest the yeast cell wall and
allow easy extraction of the pigment by
acetone or ethanol.
Enzymes Aided Extraction:
 The natural colouring extracts, especially
those obtained through aqueous extraction
or direct pressing of plant materials.
 It also contain high amount of unwanted
dissolved solids like salts, organic acids,
phenolics , sugars etc.
Microorganism Aided Extraction
 It has been established that various pigments are
produced from Monascus, Euglena etc.
 Biocolours produced by microorganisms include
Astaxanthin, Napthoquinones, Carotenoids,
Monascus pigments
 Bacteria
 Yeast
 Algae
 Mould
Microbial Production of Colour
Compounds
 Less toxic
 Less polluting
 Less health hazardous
 Non carcinogenic
 Non poisonous
 Environment friendly
 Reusable
Why we use biocolorants than
synthetic color
7/35
1. Colour Shade required.
2. Legislation of the countries in which food is
to be sold.
3. Composition of the food stuff.
4. Processing conditions.
5. pH
6. Packaging
7. Required shelf-life and storage conditions.
Factors affecting colour choice
8/35
 It is a water soluble compound and gives
colour red to blue of fruits and vegetables.
 Source :- Grapes, Strawberries, Apples, Cherry,
and red Cabbage.
 Chemically they are glycosides of flavylium or
2- phenyl- benzo- pyrylium salts.
 Sugar is present commonly glucose , glactose,
rhamnose , arabinose.
ANTHOCYANINS
Cont.9/35
 Grapes skin extracts and their colour strength
is 150 and 300 absorbance per gram.
Anthocyanins extracted from grapes , are
widely permitted.
Extraction is carried out using a dilute
aqueous solution of acid usually sulphurous
acid.
ANTHOCYANINS
10/35
1. pH
2. Heat and light
3. Oxygen
4. Sulphur dioxide(SO2)
5. Enzymes
6. Solublity
Factors affecting Stability
11/35
The pigments present are collectively known as
betalains and can be divided in to two classes as
follow
1. Red betacyanins
2. Yellow betaxanthins
BEETROOT
12/35
 Beetroots are extracted into juice by either
pressing or diffusion techniques
 Then juice is centrifuged , pasteurized and
concentrated to yield a viscous liquid
concentrated with approximately 70% sugar
and 0.5% betanin
 With spray drier powder is prepare.
Extracts and their application forms
13/35
 pH
 Heat
 Oxygen
 Light
 Water Activity
Factors affecting stability
14/35
 Source: It is present in the rhizome of the
turmeric plant (curcuma longa).Today
principal ingredient of curry powder.
 It provide both colour and flavour.
 India produce in the range of 250000 to
300000 tonnes.
 It impart colour in vegetable oil.
CURCUMIN
15/35
 There are three types of turmeric extract
namely:
1. Essential oil of turmeric
2. Turmeric oleoresin
3. Curcumin
Extract and their application forms
16/35
 This is the oil obtained by the steam
distillation of turmeric powder and contains
all the volatile flavour component of spices.
 It is present in turmeric 3-5% so small
commercial demand for this product.
1. Essential oil of turmeric
17/35
 It is obtained by solvent extraction.
 It contains 37-55% curcumin.
 It has excellent microbiological
quality,standardized organoleptic property
and freedom from contaminents.
2. Turmeric oleoresin
18/35
 It is produced by crystallization from the oleoresin.
 It has purity level 95% which is the standard
commercial available quality.
 It is pure colour.
 Antimicrobial agent.
 Lowering cholestrol by dreasing susceptibility of
LDL.
3. Curcumin
19/35
 pH
 Heat
 Light
 Sulphar dioxide(SO2)
 Solublity
Factor affecting stability
20/35
 Carotenoids are widely distributed in nature
about 3.5 tonnes of carotenoids produce in
every second.
 400 different carotenoids are identified and
many are present in our diet.
Carotenoids
21/35
 These are the yellow pigment of the leaves
and are classified as accessory pigments
which absorbs the wavelength that
chlorophyll can not absorb.
 Three kinds of xanthophyll are known:
1. Zeaxanthin
2. Canthaxanthin
3. Lutein
Xanthophyll
22/35
 Lutein produce as a by product of chlorophyll
extraction or extracted from marigold.
 Lutein is an oil soluble colour.
 A concentrated extract contains 5-12% lutein.
 Lutein is used in some lemon flavored cloudy
soft drinks and emulsified salad dressing.
Lutein
23/35
 It can be extracted from either carrots or algae.
 Natural β - carotene is available as 20-30% suspension,
solution in vegetable oil.
 Naturally extracted β - carotene is significantly more
expensive and mainly use in pharmaceutical food
industries.
 Precursor of vit . A
β - carotene
24/32
 It is a yellow-orange carotenoids obtained from seeds of
the plant Bixa-orellana.
 It is the mixer of bixin,the mono-methyl ester of
dicarboxylic carotenoid compound and
norbixin,dicarboxylic derivative of some carotenoid.
 It is also called lipstic tree.
ANNATTO
25/35
 Bixin occurs in cis or trans forms,
 On heating cis-bixin is converted to more
stable, more soluble trans- bixin.
 Bixin gives suspensions in oil and nor bixin cis
form is water soluble.
Extraction and their application
forms
26/35
 pH
 Heat and light
 Oxygen
 Sulphur dioxide
 Solublity
Factor affecting stability
27/35
 Present in green vegetable and
algae
 Degrade easily, in acidic
condition
 Replacement of central Mg ion
with Cu produce a stable
complex Cu chlorophyllin
 Chlorophyll is more stable
under alkaline condition .
 Dull in appearance.
 In history use as a sensitive
indicator.
Chlorophyll
28/35
 Source- tomato and tomato based
product,waterlemon,pink guava,papaya and
apricot.
 Lycopene is extracted from natural source by
homogenization and extraction followed by
filtration.
 Heat induces isomerization of all trans to cis
forms.
 Thermal process cause some loss of lycopene in
tomato based product.
Lycopene
29/35
Sr.
No
Biocolorant Color Source Structure Shades
1. Anthocyanin Red to Blue Grapes,appl
e
2. Annatto Yellow -
orange
Bixa
orellana
3. Curcumin Yellow Rhizome of
termeric
4. β – carotene Red Carrot,
Algae
5. Lycopene Red Tomato
6. Chlorophyll Green Green
Vagetable
7. Beetroot Red Carrot
Table 1
30/35
 Food preservatives
 Nutritional supplements
 Beverage industry
 Dairy industry
 Bakery
 Canned fruits syrup
 Meat product
 Ice cream and frozen dessert
Applications of biocolorants
31/35
 Low colour value
 Longer time make cost of dyeing
 Difficult in collection
 Lack of standardization
 Less technical knowledge of extracting
 Instability during processing
 Discolorise due to sensitive to
light,temperature,oxygen,pH
Limitation of biocolorants
32/35
 Food colorant are divided in two categories
those are covered by existing legislation and
those are not.
 By breeding
 By fermentation
 Microorganism could be made to produce
colorants in high yield by inserting genes
coding for the colorant.
Future prospects
33/35
 G.A.F. Hendry , J.D. Houghton -Natural food
Colorants. New York Pub.
 Ali Aberoumand. World Journal Of Dairy and Science
 Pritam Chattopadhyay, Sandipan Chatterjee . African
Journal of biotech.
 Manisha Parmar and Urmila Gupta. Biocolors: The
new Generation Additives.
References
34/35
Thank You
35/35

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Biocolorant (A potential food additive)

  • 2. BIOCOLORANTS A potential food additive Submitted To : Submitted By: Mrs. Ritu Sindhu Sumit Roll No. ( Food Engg.)M.Sc. (Foo.) 2/35
  • 3.  Biocolor are those coloring agent which are obtained from living source.They are generally extracted from fruits,vegetables,roots and micro organism. Examples- Annatto,Anthocyanins,Beetroots,Chlorophyll ,Carotenoids,Curcumine etc. Biocolorant 3/35
  • 4.  These are the organic colorants that are derived from natural edible sources using recognized food prepration methods.  Ex. Curcumin, Bixin, Anthocyanins, chlorophyll etc. Natural Colour 6/35
  • 5.  These are produced during cooking and processing and thus may not be of any direct importance in foods. For ex. As produced during sugar caramelization , baking etc. Browning Colour
  • 6.  Food additive colours are based on anthocyanins derived from sources such as red grapes or beet but the first additive colour were synthetic dyes which were extensively used as food colorants. Additives
  • 7.  By biotechnological approach  By microorganism  By using certain enzymes Production of Bio colour
  • 8.  Genetic modification for pigment production.  Improving the traditional methods for extraction of pigments  Microbial production of pigments  In vivo pigment production by plant tissue culture Biotechnology – Natural Colour Production
  • 9.  For the commercial extraction of astaxanthin from Phaffia rhodozyma, extracellular enzymes produced by Bacillus circulars are used.  These enzymes digest the yeast cell wall and allow easy extraction of the pigment by acetone or ethanol. Enzymes Aided Extraction:
  • 10.  The natural colouring extracts, especially those obtained through aqueous extraction or direct pressing of plant materials.  It also contain high amount of unwanted dissolved solids like salts, organic acids, phenolics , sugars etc. Microorganism Aided Extraction
  • 11.  It has been established that various pigments are produced from Monascus, Euglena etc.  Biocolours produced by microorganisms include Astaxanthin, Napthoquinones, Carotenoids, Monascus pigments  Bacteria  Yeast  Algae  Mould Microbial Production of Colour Compounds
  • 12.  Less toxic  Less polluting  Less health hazardous  Non carcinogenic  Non poisonous  Environment friendly  Reusable Why we use biocolorants than synthetic color 7/35
  • 13. 1. Colour Shade required. 2. Legislation of the countries in which food is to be sold. 3. Composition of the food stuff. 4. Processing conditions. 5. pH 6. Packaging 7. Required shelf-life and storage conditions. Factors affecting colour choice 8/35
  • 14.  It is a water soluble compound and gives colour red to blue of fruits and vegetables.  Source :- Grapes, Strawberries, Apples, Cherry, and red Cabbage.  Chemically they are glycosides of flavylium or 2- phenyl- benzo- pyrylium salts.  Sugar is present commonly glucose , glactose, rhamnose , arabinose. ANTHOCYANINS Cont.9/35
  • 15.  Grapes skin extracts and their colour strength is 150 and 300 absorbance per gram. Anthocyanins extracted from grapes , are widely permitted. Extraction is carried out using a dilute aqueous solution of acid usually sulphurous acid. ANTHOCYANINS 10/35
  • 16. 1. pH 2. Heat and light 3. Oxygen 4. Sulphur dioxide(SO2) 5. Enzymes 6. Solublity Factors affecting Stability 11/35
  • 17. The pigments present are collectively known as betalains and can be divided in to two classes as follow 1. Red betacyanins 2. Yellow betaxanthins BEETROOT 12/35
  • 18.  Beetroots are extracted into juice by either pressing or diffusion techniques  Then juice is centrifuged , pasteurized and concentrated to yield a viscous liquid concentrated with approximately 70% sugar and 0.5% betanin  With spray drier powder is prepare. Extracts and their application forms 13/35
  • 19.  pH  Heat  Oxygen  Light  Water Activity Factors affecting stability 14/35
  • 20.  Source: It is present in the rhizome of the turmeric plant (curcuma longa).Today principal ingredient of curry powder.  It provide both colour and flavour.  India produce in the range of 250000 to 300000 tonnes.  It impart colour in vegetable oil. CURCUMIN 15/35
  • 21.  There are three types of turmeric extract namely: 1. Essential oil of turmeric 2. Turmeric oleoresin 3. Curcumin Extract and their application forms 16/35
  • 22.  This is the oil obtained by the steam distillation of turmeric powder and contains all the volatile flavour component of spices.  It is present in turmeric 3-5% so small commercial demand for this product. 1. Essential oil of turmeric 17/35
  • 23.  It is obtained by solvent extraction.  It contains 37-55% curcumin.  It has excellent microbiological quality,standardized organoleptic property and freedom from contaminents. 2. Turmeric oleoresin 18/35
  • 24.  It is produced by crystallization from the oleoresin.  It has purity level 95% which is the standard commercial available quality.  It is pure colour.  Antimicrobial agent.  Lowering cholestrol by dreasing susceptibility of LDL. 3. Curcumin 19/35
  • 25.  pH  Heat  Light  Sulphar dioxide(SO2)  Solublity Factor affecting stability 20/35
  • 26.  Carotenoids are widely distributed in nature about 3.5 tonnes of carotenoids produce in every second.  400 different carotenoids are identified and many are present in our diet. Carotenoids 21/35
  • 27.  These are the yellow pigment of the leaves and are classified as accessory pigments which absorbs the wavelength that chlorophyll can not absorb.  Three kinds of xanthophyll are known: 1. Zeaxanthin 2. Canthaxanthin 3. Lutein Xanthophyll 22/35
  • 28.  Lutein produce as a by product of chlorophyll extraction or extracted from marigold.  Lutein is an oil soluble colour.  A concentrated extract contains 5-12% lutein.  Lutein is used in some lemon flavored cloudy soft drinks and emulsified salad dressing. Lutein 23/35
  • 29.  It can be extracted from either carrots or algae.  Natural β - carotene is available as 20-30% suspension, solution in vegetable oil.  Naturally extracted β - carotene is significantly more expensive and mainly use in pharmaceutical food industries.  Precursor of vit . A β - carotene 24/32
  • 30.  It is a yellow-orange carotenoids obtained from seeds of the plant Bixa-orellana.  It is the mixer of bixin,the mono-methyl ester of dicarboxylic carotenoid compound and norbixin,dicarboxylic derivative of some carotenoid.  It is also called lipstic tree. ANNATTO 25/35
  • 31.  Bixin occurs in cis or trans forms,  On heating cis-bixin is converted to more stable, more soluble trans- bixin.  Bixin gives suspensions in oil and nor bixin cis form is water soluble. Extraction and their application forms 26/35
  • 32.  pH  Heat and light  Oxygen  Sulphur dioxide  Solublity Factor affecting stability 27/35
  • 33.  Present in green vegetable and algae  Degrade easily, in acidic condition  Replacement of central Mg ion with Cu produce a stable complex Cu chlorophyllin  Chlorophyll is more stable under alkaline condition .  Dull in appearance.  In history use as a sensitive indicator. Chlorophyll 28/35
  • 34.  Source- tomato and tomato based product,waterlemon,pink guava,papaya and apricot.  Lycopene is extracted from natural source by homogenization and extraction followed by filtration.  Heat induces isomerization of all trans to cis forms.  Thermal process cause some loss of lycopene in tomato based product. Lycopene 29/35
  • 35. Sr. No Biocolorant Color Source Structure Shades 1. Anthocyanin Red to Blue Grapes,appl e 2. Annatto Yellow - orange Bixa orellana 3. Curcumin Yellow Rhizome of termeric 4. β – carotene Red Carrot, Algae 5. Lycopene Red Tomato 6. Chlorophyll Green Green Vagetable 7. Beetroot Red Carrot Table 1 30/35
  • 36.  Food preservatives  Nutritional supplements  Beverage industry  Dairy industry  Bakery  Canned fruits syrup  Meat product  Ice cream and frozen dessert Applications of biocolorants 31/35
  • 37.  Low colour value  Longer time make cost of dyeing  Difficult in collection  Lack of standardization  Less technical knowledge of extracting  Instability during processing  Discolorise due to sensitive to light,temperature,oxygen,pH Limitation of biocolorants 32/35
  • 38.  Food colorant are divided in two categories those are covered by existing legislation and those are not.  By breeding  By fermentation  Microorganism could be made to produce colorants in high yield by inserting genes coding for the colorant. Future prospects 33/35
  • 39.  G.A.F. Hendry , J.D. Houghton -Natural food Colorants. New York Pub.  Ali Aberoumand. World Journal Of Dairy and Science  Pritam Chattopadhyay, Sandipan Chatterjee . African Journal of biotech.  Manisha Parmar and Urmila Gupta. Biocolors: The new Generation Additives. References 34/35