BACTERIAL GENOME SEQUENCING PROJECT
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This presentation describes a research project which involved microbial genome sequencing at a copper mining site in Malaysia. The associated publication is available at the link in the presentation.
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Ti Plasmid
Agrobacterium tumefaciens is a soil bacterium that can transfer DNA fragments called T-DNA from its tumor-inducing (Ti) plasmid into the genome of plant cells. Scientists have harnessed this natural ability of A. tumefaciens by modifying its Ti plasmid to replace the tumor-causing genes with a gene of interest. This disarmed Ti plasmid, along with helper plasmids containing virulence genes, allows for the stable integration and expression of the new gene in plant cells. Common transformation methods using A. tumefaciens involve culturing plant explants like leaf disks with Agrobacteria containing the modified Ti plasmid, then regenerating transgenic plants from the transformed cells
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Gene tagging uses recognizable DNA fragments like T-DNA or transposons to disrupt gene function and identify genes responsible for mutant phenotypes. T-DNA tagging in plants involves random integration of Agrobacterium T-DNA that can disrupt genes and create mutants. Transposon tagging relies on the ability of transposons to move within genomes and disrupt gene function. Both techniques have been used successfully to isolate numerous plant genes involved in traits like color and development.
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Automated sequencing of genomes require automated gene assignment
Includes detection of open reading frames (ORFs)
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Agrobacterium tumefaciens is a soil bacterium that can transfer DNA fragments called T-DNA from its tumor-inducing (Ti) plasmid into the genome of plant cells. Scientists have harnessed this natural ability of A. tumefaciens by modifying its Ti plasmid to replace the tumor-causing genes with a gene of interest. This disarmed Ti plasmid, along with helper plasmids containing virulence genes, allows for the stable integration and expression of the new gene in plant cells. Common transformation methods using A. tumefaciens involve culturing plant explants like leaf disks with Agrobacteria containing the modified Ti plasmid, then regenerating transgenic plants from the transformed cells
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Gene tagging uses recognizable DNA fragments like T-DNA or transposons to disrupt gene function and identify genes responsible for mutant phenotypes. T-DNA tagging in plants involves random integration of Agrobacterium T-DNA that can disrupt genes and create mutants. Transposon tagging relies on the ability of transposons to move within genomes and disrupt gene function. Both techniques have been used successfully to isolate numerous plant genes involved in traits like color and development.
Gene prediction methods vijay
Automated sequencing of genomes require automated gene assignment
Includes detection of open reading frames (ORFs)
Identification of the introns and exons
Gene prediction a very difficult problem in pattern recognition
Coding regions generally do not have conserved sequences
Much progress made with prokaryotic gene prediction
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FASTA is a bioinformatics tool and biological database that is used to compare amino acid sequences of proteins or nucleotide sequences of DNA. It was first described in 1985 by Lipman and Pearson. FASTA performs fast homology searches to find similarities between a query sequence and sequences in a database. While similar to BLAST, FASTA is faster for sequence comparisons. It works by identifying patches of sequence similarity that may contain gaps. Some key FASTA programs include FASTA, TFASTA, FASTS, and FASTX/Y. FASTA is useful for applications like identification of species, establishing phylogeny, DNA mapping, and understanding protein function.
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Yeast vectors are useful for expressing eukaryotic proteins due to yeasts' ability to perform post-translational modifications. Common yeast species used include Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe. Vectors include integrating, episomal, replicating, centromere, and artificial chromosome plasmids. Vectors are introduced into yeast via transformation or electroporation. Expression is controlled by inducible promoters like GAL or CUP1 in S. cerevisiae and AOX1 in P. pastoris.
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shotgun sequncing
Genome sequencing is the process of determining the order of nucleotide bases - A, C, G, and T - that make up an organism's DNA. Shotgun sequencing involves randomly breaking the genome into small fragments, sequencing those pieces, and reassembling the sequence by identifying overlapping regions. It was originally used by Sanger to sequence small genomes like viruses and bacteria. There are two main methods - hierarchical shotgun sequencing for larger genomes containing repeats, and whole genome shotgun sequencing for smaller genomes.
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ti plasmid
Ti plasmids are found in Agrobacterium tumefaciens bacteria and contain genes that allow the bacteria to transform plant cells and cause crown gall tumors. The plasmid contains virulence genes that are activated by plant signals and mediate transfer of T-DNA into the plant genome. T-DNA integration results in tumor formation and production of opines that the bacteria can utilize. Ti plasmids have been engineered as vectors for plant transformation by removing oncogenes and adding gene of interest between the border sequences, allowing transformation via Agrobacterium infection of wounded plant tissues.
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DNA sequencing is a process to determine the order of nucleotides in a DNA molecule. It was discovered in the 1970s by scientists like Frederick Sanger who developed the chain termination method. This method involves DNA replication with modified nucleotides that cause the growing DNA strand to terminate at that point. The fragments are then separated by size to reveal the sequence. Automated sequencing now uses fluorescent dyes and capillary electrophoresis for faster and higher throughput sequencing. DNA sequencing has applications in medicine, forensics, and agriculture.
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This document discusses different expression systems for producing recombinant proteins, including prokaryotic, yeast, insect cell, and mammalian systems. It provides details on some commonly used expression vectors such as pGEX-3X plasmid for prokaryotic expression in E. coli, Saccharomyces cerevisiae and Pichia pastoris yeast expression systems using episomal and integrating plasmids, and baculovirus expression in insect cells using the polyhedrin promoter to drive expression of the gene of interest. The key advantages and limitations of different expression systems are also summarized.
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The document summarizes the mechanism of T-DNA transfer during Agrobacterium tumefaciens infection. It explains that T-DNA is a fragment of DNA transferred from the tumor-inducing (Ti) plasmid of A. tumefaciens into the host plant genome. The T-DNA is bordered by repeats and encodes genes that cause tumors in the plant. Virulence genes are expressed in response to plant signals and produce single-stranded T-DNA, which forms a complex with other proteins and is transported into the plant cell and integrated into the plant nuclear DNA, causing uncontrolled cell growth and tumor formation. The mechanism involves multiple virulence protein complexes and integration of T-DNA is directed by the
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Sv 40
Simian virus 40 (SV40) is a DNA virus that can cause tumors in monkeys and humans, and it was first identified as a contaminant in polio vaccines in the 1960s. SV40 has been widely used as a cloning vector due to its ability to efficiently deliver genes into a variety of cells without killing the host cell or eliciting an immune response. Future research prospects for SV40 vectors include developing recombinant versions for gene transfer applications and furthering understanding of related retroviruses.
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FASTA is a bioinformatics tool and biological database that is used to compare amino acid sequences of proteins or nucleotide sequences of DNA. It was first described in 1985 by Lipman and Pearson. FASTA performs fast homology searches to find similarities between a query sequence and sequences in a database. While similar to BLAST, FASTA is faster for sequence comparisons. It works by identifying patches of sequence similarity that may contain gaps. Some key FASTA programs include FASTA, TFASTA, FASTS, and FASTX/Y. FASTA is useful for applications like identification of species, establishing phylogeny, DNA mapping, and understanding protein function.
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Yeast vectors are useful for expressing eukaryotic proteins due to yeasts' ability to perform post-translational modifications. Common yeast species used include Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe. Vectors include integrating, episomal, replicating, centromere, and artificial chromosome plasmids. Vectors are introduced into yeast via transformation or electroporation. Expression is controlled by inducible promoters like GAL or CUP1 in S. cerevisiae and AOX1 in P. pastoris.
Whole genome sequencing
Whole genome sequencing is a technique to sequence the entire genome of an organism. It involves breaking the genome into small fragments, copying the fragments, sequencing the fragments, and reassembling the sequence data into the full genome. Key steps include isolating DNA, fragmenting it, ligating fragments into plasmids, amplifying the plasmids, sequencing the fragments using Sanger sequencing, and assembling the sequence reads into the complete genome. Whole genome sequencing allows researchers to discover coding and non-coding regions, predict disease susceptibility, and perform evolutionary studies by comparing species.
shotgun sequncing
Genome sequencing is the process of determining the order of nucleotide bases - A, C, G, and T - that make up an organism's DNA. Shotgun sequencing involves randomly breaking the genome into small fragments, sequencing those pieces, and reassembling the sequence by identifying overlapping regions. It was originally used by Sanger to sequence small genomes like viruses and bacteria. There are two main methods - hierarchical shotgun sequencing for larger genomes containing repeats, and whole genome shotgun sequencing for smaller genomes.
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ti plasmid
Ti plasmids are found in Agrobacterium tumefaciens bacteria and contain genes that allow the bacteria to transform plant cells and cause crown gall tumors. The plasmid contains virulence genes that are activated by plant signals and mediate transfer of T-DNA into the plant genome. T-DNA integration results in tumor formation and production of opines that the bacteria can utilize. Ti plasmids have been engineered as vectors for plant transformation by removing oncogenes and adding gene of interest between the border sequences, allowing transformation via Agrobacterium infection of wounded plant tissues.
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DNA sequencing is a process to determine the order of nucleotides in a DNA molecule. It was discovered in the 1970s by scientists like Frederick Sanger who developed the chain termination method. This method involves DNA replication with modified nucleotides that cause the growing DNA strand to terminate at that point. The fragments are then separated by size to reveal the sequence. Automated sequencing now uses fluorescent dyes and capillary electrophoresis for faster and higher throughput sequencing. DNA sequencing has applications in medicine, forensics, and agriculture.
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This document discusses different expression systems for producing recombinant proteins, including prokaryotic, yeast, insect cell, and mammalian systems. It provides details on some commonly used expression vectors such as pGEX-3X plasmid for prokaryotic expression in E. coli, Saccharomyces cerevisiae and Pichia pastoris yeast expression systems using episomal and integrating plasmids, and baculovirus expression in insect cells using the polyhedrin promoter to drive expression of the gene of interest. The key advantages and limitations of different expression systems are also summarized.
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Dr. Shamalamma S. presented on DNA microarrays. DNA microarrays allow thousands of genes to be compared simultaneously by attaching DNA probes to a chip which fluorescently labeled samples can bind to. The chip is then scanned to analyze gene expression levels. Applications include disease diagnosis, toxicology studies, and pharmacogenomics. While a powerful tool, microarrays have limitations such as lack of knowledge about many genes and lack of standardization.
Lectut btn-202-ppt-l5. yeast cloning vectors (1)
Yeast cloning vectors allow DNA fragments to be replicated and expressed in yeast cells. There are several types of yeast vectors including integrating plasmids (YIps) that replicate by integrating into yeast chromosomes, episomal plasmids (YEps) that replicate independently but can also integrate, and replicating plasmids (YRps) that contain an autonomously replicating sequence (ARS) and replicate at low copy numbers. Yeast artificial chromosomes (YACs) are engineered chromosomes containing telomeric, centromeric, and ARS sequences that can clone very large DNA fragments of up to 3000 kb.
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The document summarizes the mechanism of T-DNA transfer during Agrobacterium tumefaciens infection. It explains that T-DNA is a fragment of DNA transferred from the tumor-inducing (Ti) plasmid of A. tumefaciens into the host plant genome. The T-DNA is bordered by repeats and encodes genes that cause tumors in the plant. Virulence genes are expressed in response to plant signals and produce single-stranded T-DNA, which forms a complex with other proteins and is transported into the plant cell and integrated into the plant nuclear DNA, causing uncontrolled cell growth and tumor formation. The mechanism involves multiple virulence protein complexes and integration of T-DNA is directed by the
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The document provides an overview of the history and scope of bioinformatics. It discusses how bioinformatics emerged from the fields of computer science and biology. The history section outlines major developments from Mendel's work in 1865 to the sequencing of the human genome in 2001. Bioinformatics has various applications in areas like drug development, personalized medicine, and biotechnology. It also has significant scope in India, with growing job opportunities in both the public and private sectors.
Sv 40
Simian virus 40 (SV40) is a DNA virus that can cause tumors in monkeys and humans, and it was first identified as a contaminant in polio vaccines in the 1960s. SV40 has been widely used as a cloning vector due to its ability to efficiently deliver genes into a variety of cells without killing the host cell or eliciting an immune response. Future research prospects for SV40 vectors include developing recombinant versions for gene transfer applications and furthering understanding of related retroviruses.
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This document discusses genome database systems. It begins with an introduction to bioinformatics and genomes. It then discusses the background of genome databases, including some examples. The major characteristics of genome database systems are described as having high complex data, schema changes at a rapid pace, and complex queries. The key areas of data management in genome databases are discussed as non-standard data, complex queries, data interpretation, integration across databases, and uniform management solutions. Major research areas and applications that impact society are also summarized.
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Introduction
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Advantage of using binary vector
Conclusion
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Nucleic acid probes can be labeled with radioisotopes or nonisotopic labels for use in hybridization techniques. Common labeling methods include radioactive labeling with 32P or 3H, or nonisotopic labeling with biotin, digoxigenin, or fluorescein. Labeled probes are used to detect complementary DNA or RNA sequences and can be DNA, RNA, or oligonucleotide probes. Probes are prepared through various techniques such as PCR, random priming, or in vitro transcription and must be purified before use and stored appropriately.
Plasmid isolation
This document discusses the history and properties of plasmids. Plasmids are extrachromosomal DNA molecules that are able to replicate independently of a cell's chromosomal DNA. They were first observed in bacteria in the early 1950s and play important roles in processes like antibiotic resistance and virulence. The document outlines the early studies that helped characterize plasmids and describes some of their key properties, such as their circular structure and ability to exist in different conformations. It also discusses how plasmids are used as cloning vectors to amplify genes and produce proteins for applications in research, medicine, and agriculture.
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Nanoparticles helps in Controlling the Plant Diseases, application of agricultural fertilizers, pesticides, antibiotics, and nutrients is typically by spray or drench application to soil or plants, or through feed or injection systems to animals. In this context, nanotechnologies offer a great opportunity to develop new products against pests (Caraglia et al., 2011). Nanoscale devices are envisioned that would have the capability to detect and treat an infection, nutrient deficiency, or other health problem, long before symptoms were evident at the macro-scale. The overall goal of this Nanoparticles is to reduce the number of unnecessary problems in agriculture (Thomas et al., 2011). In the management aspects, efforts are made to increase the efficiency of applied fertilizer with the help of nano clays and zeolites and restoration of soil fertility by releasing fixed nutrients (Dongling Qiao, et al., 2016). Nanoherbicides are being developed to address the problems in perennial weed management and exhausting weed seed bank. Bioanalytical Nanosensors are utilized to detect and quantify minute amounts of contaminants like viruses bacteria, toxins bio-hazardous substances etc. in agriculture and food systems (Tothill EI, 2011).
In this way, nanotechnology can be used as an innovative tool for delivering agrochemicals safely. More research should be done on the potential adverse effects of nanomaterials on human health, crops and the environmental safety. It is a challenge to Government and private sector as they have to ensure the acceptance of Nano foods. For it to flourish, continuous funding and understanding on the part of policy makers and science administrators, along with reasonable expectations, would be crucial for this promising field.
Microbiology
Microbiology is the study of microscopic organisms like bacteria, fungi, protozoa and viruses. The document outlines the key disciplines of microbiology including bacteriology, mycology, and virology. It discusses the importance of microbes in everyday life such as in food production, medicine, and the environment. The document also summarizes the contributions of pioneering microbiologists like Hooke, van Leeuwenhoek, Pasteur, Koch, and others. It describes techniques used in microbiology like staining, culture methods, and biochemical tests. Finally, it discusses the many applications of microbes in industry, biotechnology, medicine, and environmental remediation.
Metal ion transport and storage
This document summarizes metal ion transport and storage in biological systems. It discusses the general properties of transport systems like ionophores, ion channels, and ion pumps. Specific mechanisms for transporting ions like sodium, potassium, iron, and calcium are described. Metal storage is achieved through proteins like ferritin and metallothionein. Problems associated with transporting and storing metal ions across membranes are also highlighted.
Isolation and Characterization of Halophilic Bacteria from Sundarban Soil Deb...
Isolation and Characterization of Halophilic Bacteria from Sundarban Soil Deb...SSR Institute of International Journal of Life Sciences
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
microbial studies on coral mucus
coral mucus is the method of protection used by coral reef in sea water since it's a species specific a little studies hold on it and there's a lot of research to go on it.
Genotoxicity
Genotoxicity refers to the ability of substances to damage genetic material like DNA. Genotoxicity testing aims to identify substances that may induce DNA damage through in vitro and in vivo assays. The Ames test is a widely used bacterial reverse mutation assay to assess mutagenicity. Mammalian cell assays like the micronucleus test and comet assay are also employed to study chromosomal damage and DNA breakage in vivo. Standard test batteries from organizations like OECD provide guidelines for various assays to thoroughly evaluate genotoxic potential of new chemicals and drugs. Genotoxic agents can cause cancer, mutations and birth defects by interacting with and damaging DNA.
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MICROPALEONTOLOGY : Introduction and its importance.pptx
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Mechanism of Zinc solubilization by Zinc Solubilizing bacterias
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International Journal of Biometrics and Bioinformatics(IJBB) Volume (3) Issue...
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Isolation and Characterization of Halophilic Bacteria from Sundarban Soil Deb...
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PHYLOGENETICS WITH MEGA
The MEGA software is one of the most widely used software tools in molecular taxonomy and bioinformatics. This module describes how MEGA can be employed in a classroom setting to teach the fundamentals of molecular taxonomy.
Plasmid DNA
This lecture is intended as an introduction to the fundamental concepts associated with plasmid DNA. Plasmids can be applied as vectors in Genetic Engineering for the production of recombinant proteins as well as the construction of genomic libraries for DNA sequencing projects.
ISO9001:2015
ISO 9001 is an international standard for quality management systems that can be certified. It provides requirements for ensuring products and services meet customer and regulatory requirements through an effective quality management system. Over 1 million organizations in 170+ countries are certified to ISO 9001. It emphasizes continual improvement and having processes to understand customer needs and satisfaction, as well as manage risks and opportunities.
Reverse Transcription
Reverse transcription of RNA, which refers to the conversion of the RNA template into its complimentary DNA strand (cDNA) is an essential step in the analysis of gene transcripts.
cDNA can be sequenced, cloned and applied to estimate the copy number of specific genes in order to characterize and to validate gene expression.
Reverse Transcription of RNA
Reverse transcription of RNA is a process whereby RNA, typically messenger RNA is converted into complimentary DNA. The process was discovered by Howard Temin and John Baltimore when they observed that certain RNA viruses could revert to DNA. This was an important discovery that led to the discovery of enzymes classified as reverse transcriptases. Today Reverse Transcription is routinely applied in molecular biology laboratories to obtain the stable cDNA version of RNA for downstream analysis.
Breeding Plants using Chemical Mutagens
Plants can be genetically modified by the use of chemical mutagens. Chemical mutagens interact with the DNA and cause mutations in the genome. Chemical mutagenesis is a powerful tool which can be applied for the development of plants with desired agronomic traits.
Genome Editing with TALENS
Genome editing is one of the most important tools which supports genetic engineering. It is based on the naturally occurring mechanism of DNA recombination which involves the initiation of breaks with the double stranded DNA followed by repair by the endogenous DNA polymerases.
Conventional techniques such as gene knockouts using P-elements and transposable genetic elements have been superseded by more accurate genome editing methods such as TALENs and CRISPR/Cas.
PRINCIPLE OF CRISPR GENOME EDITING
Genome editing with the CRISPR-Cas9 system has become one of the major tools in modern biotechnology. This slide share discusses the fundamentals in a simple, easy to understand format.
An overview of the Pharmaceutical Industry
This module is intended to introduce the students of biotechnology to obtain an overview of the pharmaceutical industry. The concept of clinical trials is discussed in brief.
Effluent treatment
This lecture note describes the process of Effluent Treatment (ET). Emphasis is give to the biological aspects of ET. Free to reuse, remix, modify and share for non-commercial and commercial purposes.
Molecular Breeding in Plants is an introduction to the fundamental techniques...
Molecular Breeding in Plants is an introduction to the fundamental techniques...UNIVERSITI MALAYSIA SABAH
The document discusses molecular genetics and breeding in plants. It begins by introducing Arabidopsis thaliana as a model plant and describes its small genome size, which was advantageous for early genome sequencing efforts. It notes that the A. thaliana genome contains 5 chromosomes totaling 115 Mbp and encodes 25,498 genes. The document then discusses various aspects of the A. thaliana genome structure, organization, and chromosomes. It also briefly describes the chloroplast and mitochondrial DNA structures. The remainder of the document focuses on introducing concepts in plant genetics and molecular breeding techniques.More from UNIVERSITI MALAYSIA SABAH (11)
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ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
如何办理(uvic毕业证书)维多利亚大学毕业证本科学位证书原版一模一样
原版纸张【微信：741003700 】【(uvic毕业证书)维多利亚大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
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◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
3D Hybrid PIC simulation of the plasma expansion (ISSS-14)
3D Particle-In-Cell (PIC) algorithm,
Plasma expansion in the dipole magnetic field.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Shallowest Oil Discovery of Turkiye.pptx
The Petroleum System of the Çukurova Field - the Shallowest Oil Discovery of Türkiye, Adana
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
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ESR spectroscopy in liquid food and beverages.pptx
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Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...
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Equivariant neural networks and representation theory
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aziz sancar nobel prize winner: from mardin to nobel
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BACTERIAL GENOME SEQUENCING PROJECT
- 1. Genome Sequencing Project: Bacillus thuringiensis MCMY1 Research Collaboration with
- 2. The Mamut Copper Mine
- 4. Research Questions • What are the genetic mechanisms which enable microbes to adapt to acid mine drainage? • Is there evidence of horizontal gene transfer? • What are the specific genetic features associated with tolerance to acid and metal ions?
- 5. Objectives • Isolation of microbes. • Screening for copper tolerant species. • Biochemical characterization. • Morphological characterization. • Genome sequencing and analysis.
- 6. Material and Methods Sample collection Microbial isolation Characterization Genome Sequencing
- 7. Sample Collection Soil samples were collected at a depth of 10 cm below the surface.
- 8. This study focused of B. thuringiensis • Molecular ribotyping based on the 16S rRNA sequence was used to identify an isolated bacterium which was tolerant to copper. • The bacterium was identified :Bacillus thuringiensis.
- 9. Inhibition by Copper CONTROL 0.5 mM 1.0 mM 1.5 mM Axenic cultures of isolated microbes were carried out on Nutrient Agar with different concentrations of Metal ions..
- 10. Nitrogen Fixation Colonies grow on Jensen’s media
- 11. Phosphate Solubilization Positive test for Phosphate solubilization
- 12. Morphology in Culture Media
- 13. Microscopy
- 14. Microscopy
- 15. Genome Sequencing Pacific Biosciences RS2 Single Molecule Real Time (SMRT) Sequencer.
- 16. Genome Data • No evidence of plasmid. • Genes associated • The genome encodes genes associated with the biosynthesis of exo-polysaccharides.
- 17. Genome Data
- 18. References
- 19. Acknowledgements This research was funded by the Ministry of Higher Education, Government of Malaysia, Research Grant No. FRGS0455 Permission to conduct sampling at the Mamut Copper Mine site was obtained from the Department of Mines