3. HISTORY
• 1903 – Martin – Fistula technique of VE using silver
wires (gonococcal epididymal obstruction)
• 1919 – Quinby – VV for Vasectomy reversal
• 1977 – Silber – Two-layered microsurgical vasal
anastomosis technique
• 1978 – Silber – VE to single epididymal tubule
• 1998 – Goldstein – Microdot microsurgical VV
4. VASOVASOSTOMY
• 2-6% vasectomized men seek reversal
• 6% azoospermic men – Iatrogenic-Obstructive
• Document adequate spermatogenesis – prior history
of natural fertility
Indications
• Vasectomy reversal – Vast majority
• Iatrogenic vasal injury repair – 2° to Herniorraphy
5. Patient Preparation
• GA preferred
– Operating microscope magnifies patient movement
– If VE required – surgery time may exceed 4-5hr
• Regional & local possible in co-operative patient
• Position – Supine & towards foot-end of the table
• Surgeons to support ulnar part of hands, wrists, &
forearms – minimize tremor
7. Is Microscope Necessary?
• Loupes – ×2.5 to ×4 magnification
• Visualization of the inner lumen of the vas deferens
requires ×16 magnification
• Microscope
– the depth of focus is clear
– Light is constantly supplied directly to the patient
– Instrument rests on a stand & is immobile – Surgeon
can move his neck
9. The Goldstein Vas approximating clamp
90° Vas cutting forceps
Foam from suture packets with dots
to secure the microneedles after use
Microneedle
With sharp point
11. Surgical Approaches
Scrotal Approach
• Bilateral high vertical scrotal incisions
• Ideal for vasectomy reversal
• Testis delivered with TV intact
• Min. 1cm lateral to penis-base
• Can be extended to external ring
Inguinal Approach
• Suspected inguinal obstruction – prior Herniorrhaphy /Orchiopexy
12. Vas Preparation
• Vas mobilized to allow tension-free anastomosis without
stripping adventitia (vasal vessels)
• Stay close to Vas – avoid Testicular artery injury
• Vasal Gap – Separate cord structures from vas
– Blunt dissection till internal ring
– Dissect vas free of attachment to epididymal tunica
– Cut floor of inguinal canal & rerout vas
– Dissect epididymis off testis from vasoepididymal junction to
caput
13. Dissect vas free of attachment to epididymal tunica
Dissect epididymis off testis from
vasoepididymal junction to caput
Upto 10cm length can be gained
14. • Testicular end of the vas is cut transversely
– Ultrasharp knife drawn through a slotted nerve-holding clamp
– Perfect 90° cut
• Vasal Fluid sampled
15. • Slide Preparation – Vasal fluid + NS/RL
• Abdominal end of Vas prepared similarily
– lumen gently dilated with microvessel dilator
– cannulated with a 24-gauge angiocatheter sheath
– Injection of NS/RL to confirm patency
– Vas is recut to obtain a fresh surface
– Minimum instrumentation of mucosa
16. Bull’s eye appearance of healthy cut vas
Gently open with microforceps
NO dilatation
17. Vasal Fluid Examination
• Vasovasostomy
– Copious crystal clear watery fluid ± sperm
– Copious fluid with sperms / sperm heads
– Scant fluid with granuloma & sperm on barbotage
• Vasoepididymostomy
– Thick paste-like with no sperm/sperm heads
– Scant fluid with no granuloma Or sperm/sperm heads
– Fluid with occasional grape-like clusters of sperm heads
19. Multiple Vasal Obstruction
• Probe abdominal vas with 0 polypropylene to locate
level of obstruction
• Within 5cm – excise & do a single VV
• Two VV – intervening segment devascularizes
• If not possible –
– Preserve sperm
20. Varicocelectomy + Vasovasostomy
• Do VV/VE first → re-assess postoperative semen quality
• Varicocelectomy – at ≥6mon – arteriovenous channels
form across anastomosis
• Can be done together –
– Microscope be used
– Testicular artery preserved
• Cremasteric & peri-arterial venous channels divided
21. Anastomotic Principles
1. Accurate mucosa-to-mucosa approximation
2. Leakproof anastomosis
3. Tension-free anastomosis
4. Good blood supply
5. Healthy mucosa and muscularis
6. Good atraumatic anastomotic technique
22. Microsurgical Multilayer Microdot Method
• Microdots allow precise placement of microsutures
• Double dot at the 12 o’clock to maintain orientation
• Separates planning from placement of suture
• Needle exit marked
• Six mucosal sutures
23. Techniques to Stabilize the Vas
1. Goldstein vas approximating clamp
2. Micro-wipe technique
3. Stay sutures
24. • Double-arm suture – inside-out needle placement
• Anterior Layer → Rotate vas 180° → Posterior layer
• 10-0 nylon double-arm for inner layer
• 9-0 nylon for outer muscular layer
• Irrigate with heparinized saline before last suture
• Approximate vasal sheath
28. VASOEPIDIDYMOSTOMY
Indications
1. Post-Vasectomy/Iatrogenic Obstructive Azoospermia
2. Complete spermatogenesis in one testis (prior biopsy
or highly positive S. Antisperm Ab) + no sperm in vas
+ no vasal/ED obstruction
Microsurgical VE – technically demanding
Dilated epididymal tubule – 0.2mm in diameter
Wall thickness of epididymal tubules – 30 microns (0.03mm)
31. Identify Target Tubule
Target tubule
parallel to incoming
abdominal vas
Avoid tubule
perpendicular to
abdominal vas
Avoid off-center,
irregular, or
lengthy defect
Measure & choose
most distal tubule
for anastomosis
32. Vas Preparation
• Angled Marks Vas-cutting forceps – 15°
– allows Vas to “lay down” over the defect
– provides larger elliptical lumen for intussusception
• Confirm abdominal-vas patency
• Confirm distal vas position
33. Delivery of Target Tubule
• Observe tubules through the epididymal tunic
– Change in diameter of the tubules
– Color of the fluid within
• Find the best tubule, correctly oriented in a window
without overlying vessels
• Incise outer tunica → free target tubule → it should
protrude from adjacent tubules
• Tubule should be at the center of tunica window
38. Success
• VV – overall > 95% patency & > 70% pregnancy rate
• VE – overall 70% patency & 50% pregnancy rate
• Sperm in ejaculate sooner with a VV than VE
– VV – 75% patency at 3months
– VE – 23% at 3mon → 62% at over 1yr
• Late Obstruction rates –
– VV – 5-12%
– VE – 10% with LIVE, 25% with end-to-side/end anastomoses
39. Robotic-assisted VV
• Elimination of tremor & improved stability
• Surgeon ergonomics/decreased surgeon fatigue
• Scalability of motion
• Three-dimensional high-definition visualization
• Manipulate 3 instruments & camera simultaneously
• Not requiring a specialty skilled microsurgical assistant
• Potential of improving operative times
40. • Allows surgeon to back out of console & re-engage
on exact same field
Cons
• Decreased Magnification
• Increased cost
41. Postop Management
• Complications – Bleed, Infection, Hydrocele, Testicular
atrophy (rare)
• Scrotal Support for 6wk at all times (in sleep too) →
Support during strenous activity till pregnancy
• No strenous work for 3wk
• No intercourse or ejaculation for 3wk
43. TURED
• Trans-Urethral Resection of the Ejaculatory Ducts
• TRUS-guided aspiration of cystic/dilated ejaculatory
ducts or seminal vesicles
• If motile sperm found → cryopreserved
– 2-3 ml Indigo-carmine diluted + contrast instilled
– Decision of TURED based on imaging
• No sperm in bilateral Vas + ED obstruction → abandon
44. Technique
• Cold knife incision alone → re-obstruction
• Cutting loop + anterior push on prostate per rectum
• EDs course b/w bladder-neck & veru → exit along
lateral aspect of veru
• Preserve bladder neck, striated sphincter, & rectal
mucosa
• Hemivasotomies closed using microsurgical technique
45.
46. Complications
Complications
• Reflux of urine into EDs, Vas, and Seminal Vesicles
• Acute & Chronic Epididymitis
• Retrograde Ejaculation
Results – Not Favoured
• Increased semen volume – in two-third cases
• Sperm in ejaculate – 50%