This study assessed the knowledge and attitudes regarding care of patients with HIV/AIDS among 207 nurses working at BPKIHS, Nepal. The study found that the majority (92.3%) of nurses had moderately adequate knowledge of HIV/AIDS patient care. Over half (50.7%) of nurses reported having a favorable attitude toward caring for HIV/AIDS patients. No significant associations were found between knowledge, attitude, and socio-demographic or training variables. Knowledge was found to have a significant positive correlation with attitude. The study concluded that nurses generally have adequate knowledge and favorable attitudes, but continuous education is still needed to ensure non-stigmatizing, high-quality care of HIV/AIDS patients.