This file is uploaded for your education purpose and This presentation name is Active transport. And it's types Primary and secondary transport Sodium glucose transporter Potassium Pump

1. ACTIVE TRANSPORT: PRIMARY
ACTIVE TRANSPORT AND
SECONDARY ACTIVE
TRANSPORT
MR KHAN
M.SC (MICROBIOLOGY)
SESSION 2023-25

2. ACTIVE TRANSPORT
• Molecules move against the low concentration to high concentration.
• It requires energy.
• Exhibit saturation kinetics.

3. TYPES OF ACTIVE TRANSPORT
Active transport is divided into two types .
1. Primary Active Transport
2. Secondary Active Transport

4. PRIMARY ACTIVE TRANSPORT
• It is also called direct active transport because directly uses metabolism
energy (ATPs) to transport molecules across a membrane.
• Na+ , K+ ,Ca2+ ions transport by Primary active transport.
• Sodium potassium Pump
• Calcium Pump
• Hydrogen Pump
• Hydrogen / Proton Pump

5. SECONDARY ACTIVE TRANSPORT
• It is also known as coupled transport and cotransport.
• In secondary active transport, there is no directly coupling of ATP.
• But it depend upon the electrochemical potential difference created by
pumping of ions in/out of the cell .
• Best example of secondary active transport – Sodium glucose transporters

6. SODIUM GLUCOSE TRANSPORTERS
• Cotransporters are a family of glucose transporter found in the small
intestine.
• Firstly Na+/K+ ATPase pumps out 3 sodium ions and bringing in 2
potassium ions.
• This action creates a downhill sodium ion gradient inside the cell wall.
• Sodium-glucose transporters uses sodium ion gradient created by
Na+/K+ ATPase pump to transport glucose across the membrane.

8. SODIUM POTASSIUM PUMP
• Sodium potassium pump also known as Na+/K+ -ATPase is an enzyme found in the plasma membrane of
all animal cells.
• The Na+/K+ -ATPase enzyme is a ion pump. That pumps out 3 Na+ ions from cells while pumping 2 Ka+
into cells, both against their concentration gradient.
• This pump is called a P-type ion pump. Because the ATP interactions phosphorylate the transport
protein and causes a change in its confirmation.
• This pumping is active (i.e. It uses energy from ATP) and is important for cell physiology.
• It has antiporter like activity, but it is not a true antiporter.

9. FUNCTIONS
• Maintains volume of the cell.
• Essential for oxygen utilisation by the kidneys.
• Causes negative electrical charge inside the cell-electrogenic pump.
• Maintain sodium potassium concentration difference across the cell membrane.

10. MECHANISM OF SODIUM POTASSIUM PUMP
• When the protein binds 3 Na+ ions on the inside of the cell and becomes
phosphorylated.
• Due to phosphorylation it shifts from the E1 confirmation to the E2 confirmation.

12. THANK YOU 😊