This study analyzed the availability of bioenergy resources and potential for biogas generation from dairy manure in North Kolkata, India. A survey found over 9,000 buffaloes producing over 1,000 tons of manure daily on 164 dairy farms. The manure could generate over 4,000 cubic meters of biogas and 7,500 kWh of electricity per day, meeting the area's cooking energy demand of 52 GJ/day and electricity demand of 2,350 kWh/day with 375 kWh of excess electricity available for other uses. A large-scale anaerobic digester could utilize all available manure to meet local energy needs through biogas and grid-connected electricity while generating additional revenue.