Esta es la petición que presento en febrero de 2011 el Centro por la Ciencia de Interés Público (Center for Science in the Public Interest) ante la Administración de Alimentos y Medicamentos (Food and Drug Administration) de los Estados Unidos para que se prohíba el uso del colorante Caramelo IV por contener el compuesto cancerígeno 4-Metilidimazol. La petición presenta la evidencia existente sobre el potencial cancerígeno del compuesto 4-Metilidimazol (ver DOC1), los niveles máximos que se están recomendando como tolerables en California para la ingesta de este compuesto (ver DOC2) y las cantidades de 4-Metilidimazol que se han registrado en bebidas de Cola que rebasan por mucho lo recomendado (ver DOC3).
Chemsitry of Natural Products-Flavonoids and quercetinSurendraKumar338
# Quercetin is the most abundant dietary flavonoid. It has been linked to improved exercise performance and reduced inflammation, blood pressure, and blood sugar level.
# Flavonoids any of large class of plant pigments having a structure based on or similar to that of flavone.
Louis- Camille Maillard described a browning reaction between reducing sugars and amino groups. Despite not being the first to report the reaction, Maillard was the first to realize the significance of the reaction in areas as diverse as plant pathology, geology and medicine
Deoxy sugars, 6-Deoxy-hexoses
(methyl pentoses or hexomethyloses)
alpha-L-Rhamnose (6-deoxy-L-mannose),D-Digitoxose
a component of the sugar part of Digitalis glycosides
Keller Killiane test ,DISACCHARIDES, Maltose (malt sugar)
Lactose (milk sugar) LACTULOSE, Polysaccharide
Chemsitry of Natural Products-Flavonoids and quercetinSurendraKumar338
# Quercetin is the most abundant dietary flavonoid. It has been linked to improved exercise performance and reduced inflammation, blood pressure, and blood sugar level.
# Flavonoids any of large class of plant pigments having a structure based on or similar to that of flavone.
Louis- Camille Maillard described a browning reaction between reducing sugars and amino groups. Despite not being the first to report the reaction, Maillard was the first to realize the significance of the reaction in areas as diverse as plant pathology, geology and medicine
Deoxy sugars, 6-Deoxy-hexoses
(methyl pentoses or hexomethyloses)
alpha-L-Rhamnose (6-deoxy-L-mannose),D-Digitoxose
a component of the sugar part of Digitalis glycosides
Keller Killiane test ,DISACCHARIDES, Maltose (malt sugar)
Lactose (milk sugar) LACTULOSE, Polysaccharide
Glycerol is a chemical compound that is generally non-toxic, sweet tasting, vicious liquid. Glycerol is completely soluble in water and alcohol.Glycerol was first obtained as a bi-product of soap manufacture through saponification of fats. Glycerine is used in the cosmetics and medicinal industries.
Coca-Cola is increasingly criticized for its use of unhealthy ingredients and poor business practices. In spite of its increasingly bad reputation, some details of its operations remain little-known...
Food browning due Maillard chemical reaction occurs between amino acidsrita martin
The browning of these food stuffs is due to Maillard Reaction creates flavor and changes the color of food generally only begin to occur above 285°F occurs between amino acids
Introduction and defination
Classification
Reducing sugars
Non-reducing sugars
General properties
Common disaccharides
1) sucrose
Origin
Structure
Properties
Function
Structural elucidation and isolation of glycoside, purine and flavanoidsSana Raza
Unit three of chemistry of natural products, consist of the method of extraction, isolation and structure elucidation of Glycoside(digoxin), Flavanoids( quercetin), Lignans( Podophyllotoxin), Purines (caffeine)
Carbohydrates full notes slides for exam and presentation,
carbohydrates notes for biochemistry and pharmacognosy,
best medical notes and presentation slides for medical and pharmacy students,
Established in 1986 and based in London, UK, UHY is a network of independent accounting and consulting firms with offices in over 270 major business centres in 86 countries. Our services and teams are tailored to suit the culture of each client including publicly listed corporations, large and medium-sized companies, privately owned businesses, not-for-profit and public organisations.
PROFESSIONALISM, QUALITY, INTEGRITY, INNOVATION
Our values are echoed in the standard of the firms that have achieved membership in UHY. Our member firms understand that business people need a wide range of services and knowledge of local conditions to help them create, operate and dispose of ventures across the globe. The UHY worldwide network was formed in response to the needs of our clients; our global relationships have come to define the next level of service that our clients have earned and expect.
WebLion Hosting: Leveraging Laziness, Impatience, and HubrisErik Rose
Behind the scenes of WebLion's Plone hosting service, which uses Debian packages and a custom repository to deliver reliable, unattended updates to a cluster of heterogeneous departmental virtual servers. And it's all available for your own use for free.
Resumo das principais funcionalidades para developers, bem como novos recursos para administradores como o grid link for rac e o active cache. também abordamos recursos de grid como o coherence
EdCamp News & UpDates
By now, it is safe to say that EdCamp is fast consolidating itself as the conference you go to when you don't want to sit passively in an audience of your peers and listen to the wisdom of the "guru" of the moment.
You go to an EdCamp near you.
You want to be active, share what you are doing in your classroom, look for real world solutions to real world problems, collaborate and cooperate with peers.
You go to an EdCamp near you...
EdCamp is free, democratic, conversation-based, participant-driven professional development for teachers by teachers...
According to the EdCamp Foundation, an authentic Edcamp has the following features:
"free: Edcamps should be free to all attendees. This helps ensure that all different types of teachers and educational stakeholders can attend.
non-commercial and with a vendor free presence: Edcamps should be about learning, not selling. Educators should feel free to express their ideas without being swayed or influenced by sales pitches for educational books or technology.
hosted by any organization or anyone:
Anyone can host an Edcamp. School districts, educational stakeholders, and teams of teachers have hosted Edcamps. YOU could be the next Edcamp organizer!
made up of sessions that are determined on the day of the event:
<strong>Edcamps do not have scheduled presentations</strong>.
During the morning of the event, the schedule is created in conjunction with everyone there. I know it sounds crazy, but it works! Sessions end up being spontaneous, interactive, and responsive to participants’ needs.
events where anyone who attends can be a presenter:
Anyone who attends an Edcamp is able to be a presenter. All teachers and educational stakeholders are viewed as professionals worthy of sharing their expertise in a collaborative setting.
reliant on the law of two feet that encourages participants to find a session that meets their needs:
As anyone can host a session, it is critical that participants can actively self-select the best content and sessions. <strong>Edcampers are encouraged to leave sessions that do not meet their needs.</strong>
This provides a uniquely effective way of “<strong>weeding out</strong>” sessions that are not based on appropriate research or not delivered in an engaging format."
Source: <a>http://edcampfoundation.org/</a>
You go to an EdCamp near you...
Glycerol is a chemical compound that is generally non-toxic, sweet tasting, vicious liquid. Glycerol is completely soluble in water and alcohol.Glycerol was first obtained as a bi-product of soap manufacture through saponification of fats. Glycerine is used in the cosmetics and medicinal industries.
Coca-Cola is increasingly criticized for its use of unhealthy ingredients and poor business practices. In spite of its increasingly bad reputation, some details of its operations remain little-known...
Food browning due Maillard chemical reaction occurs between amino acidsrita martin
The browning of these food stuffs is due to Maillard Reaction creates flavor and changes the color of food generally only begin to occur above 285°F occurs between amino acids
Introduction and defination
Classification
Reducing sugars
Non-reducing sugars
General properties
Common disaccharides
1) sucrose
Origin
Structure
Properties
Function
Structural elucidation and isolation of glycoside, purine and flavanoidsSana Raza
Unit three of chemistry of natural products, consist of the method of extraction, isolation and structure elucidation of Glycoside(digoxin), Flavanoids( quercetin), Lignans( Podophyllotoxin), Purines (caffeine)
Carbohydrates full notes slides for exam and presentation,
carbohydrates notes for biochemistry and pharmacognosy,
best medical notes and presentation slides for medical and pharmacy students,
Established in 1986 and based in London, UK, UHY is a network of independent accounting and consulting firms with offices in over 270 major business centres in 86 countries. Our services and teams are tailored to suit the culture of each client including publicly listed corporations, large and medium-sized companies, privately owned businesses, not-for-profit and public organisations.
PROFESSIONALISM, QUALITY, INTEGRITY, INNOVATION
Our values are echoed in the standard of the firms that have achieved membership in UHY. Our member firms understand that business people need a wide range of services and knowledge of local conditions to help them create, operate and dispose of ventures across the globe. The UHY worldwide network was formed in response to the needs of our clients; our global relationships have come to define the next level of service that our clients have earned and expect.
WebLion Hosting: Leveraging Laziness, Impatience, and HubrisErik Rose
Behind the scenes of WebLion's Plone hosting service, which uses Debian packages and a custom repository to deliver reliable, unattended updates to a cluster of heterogeneous departmental virtual servers. And it's all available for your own use for free.
Resumo das principais funcionalidades para developers, bem como novos recursos para administradores como o grid link for rac e o active cache. também abordamos recursos de grid como o coherence
EdCamp News & UpDates
By now, it is safe to say that EdCamp is fast consolidating itself as the conference you go to when you don't want to sit passively in an audience of your peers and listen to the wisdom of the "guru" of the moment.
You go to an EdCamp near you.
You want to be active, share what you are doing in your classroom, look for real world solutions to real world problems, collaborate and cooperate with peers.
You go to an EdCamp near you...
EdCamp is free, democratic, conversation-based, participant-driven professional development for teachers by teachers...
According to the EdCamp Foundation, an authentic Edcamp has the following features:
"free: Edcamps should be free to all attendees. This helps ensure that all different types of teachers and educational stakeholders can attend.
non-commercial and with a vendor free presence: Edcamps should be about learning, not selling. Educators should feel free to express their ideas without being swayed or influenced by sales pitches for educational books or technology.
hosted by any organization or anyone:
Anyone can host an Edcamp. School districts, educational stakeholders, and teams of teachers have hosted Edcamps. YOU could be the next Edcamp organizer!
made up of sessions that are determined on the day of the event:
<strong>Edcamps do not have scheduled presentations</strong>.
During the morning of the event, the schedule is created in conjunction with everyone there. I know it sounds crazy, but it works! Sessions end up being spontaneous, interactive, and responsive to participants’ needs.
events where anyone who attends can be a presenter:
Anyone who attends an Edcamp is able to be a presenter. All teachers and educational stakeholders are viewed as professionals worthy of sharing their expertise in a collaborative setting.
reliant on the law of two feet that encourages participants to find a session that meets their needs:
As anyone can host a session, it is critical that participants can actively self-select the best content and sessions. <strong>Edcampers are encouraged to leave sessions that do not meet their needs.</strong>
This provides a uniquely effective way of “<strong>weeding out</strong>” sessions that are not based on appropriate research or not delivered in an engaging format."
Source: <a>http://edcampfoundation.org/</a>
You go to an EdCamp near you...
For Self-Published Authors. Creative Content Opps. Bookexpo America uPublishU...Susannah Greenberg
Creative Content Opportunites. With Susannah Greenberg Public Relations; Miral Sattar, BiblioCrunch; Steve Wilson, FastPencil; and bestselling author Kailin Gow. For self-publishing and/or entrepreneurial authors seeking to maximize the impact of their publishing, marketing and book pr. Books. Publishing. Authors. A presentation at Bookexpoamerica 2014 uPublishU.
Condor overview - glideinWMS Training Jan 2012Igor Sfiligoi
An overview of the Condor Workload Management System, with emphasis on how it is used within the glideinWMS.
Part of the glideinWMS Training session held in Jan 2012 at UCSD.
The event page is http://hepuser.ucsd.edu/twiki2/bin/view/Main/GlideinFrontend1201
Video available at http://www.youtube.com/watch?v=tpaedg09VMM
See you at the ISC 2014 from April 2 to 4th at the Sands Expo Las Vegas!
Our pavilion is located in the designated area for Global Expo on the RIGHT side of the exhibit hall from the entrance, please come by and say HELLO at the KOTRA booth #40718
Annual DEDO talk on E-Commerce trends, and the basic of design and web development to get your new store off the ground.
With more than $370 billion dollars (US only) in projected e-commerce revenue in 2017, and more than 43% of that coming from mobile sales, are you ready? More than 79% of retailers have said that they aren't prepared.
Remember, that your most valuable asset is a users time.
Mercury Levels in Locally Manufactured Mexican Skin Lightening Creams v2zq
Mercury Levels in Locally Manufactured Mexican Skin Lightening Creams - Resources for Healthy Children www.scribd.com/doc/254613619 - For more information, Please see Organic Edible Schoolyards & Gardening with Children www.scribd.com/doc/254613963 - Gardening with Volcanic Rock Dust www.scribd.com/doc/254613846 - Double Food Production from your School Garden with Organic Tech www.scribd.com/doc/254613765 - Free School Gardening Art Posters www.scribd.com/doc/254613694 - Increase Food Production with Companion Planting in your School Garden www.scribd.com/doc/254609890 - Healthy Foods Dramatically Improves Student Academic Success www.scribd.com/doc/254613619 - City Chickens for your Organic School Garden www.scribd.com/doc/254613553 - Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica www.scribd.com/doc/254613494 - Simple Square Foot Gardening for Schools - Teacher Guide www.scribd.com/doc/254613410 - Free Organic Gardening Publications www.scribd.com/doc/254609890 ~ mdpi.com
The impurities in pharmaceuticals are unwanted chemicals that remain with the active pharmaceutical ingredients
(APIs) or develop during formulation or upon aging of both API and formulation.
Este es uno de los estudios científicos, recientemente publicados, que se ha enfocado en registrar la cantidad del compuesto 4-Metilidimazol que se encuentra en las bebidas de Cola y en la cerveza oscura. El compuesto 4-Metilidimazol es un subproducto cancerígeno que se encuentra en el colorante Caramelo IV utilizado en estas bebidas. El estudio reconoce que la mayor ingesta que se da de este compuesto es a través de las bebidas de Cola. Estima que en los Estados Unidos el consumo promedio diario de bebidas de Cola puede representar una ingesta de 342 microgramos diarios de 4-Metilidimazol. Esta cantidad significa varias veces el nivel máximo recomendado como seguro de ingesta de este compuesto sugerido por la autoridad de California, para todo un día que es de 16 microgramos al día. El estudio reporta que una sola lata de bebida de Cola puede contener entre 12.95 y 214.55 microgramos de 4-Metilidimazol.
Herbal cosmetics have been into usage from time immemorial so has been the use of Ayurvedic medicines. Ayurveda which means the complete knowledge for long life has been very popular these days on account of its minimum or zero side effects with considerable power of curing. Similarly herbal cosmetics have been of great value because of the least harm they cause to the skin and the radiance they add to the skin.
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Techno-economic analyses of specific lactic acid production processes, presenting capital investment breakdown, raw materials consumed and operating costs. Know more at www.intratec.us/products/lactic-acid-production-processes
its a case study about coca cola business practicese in colombia, mexico and india. it is helpful your presentation and project work. it is very attractive slides, hope you will like it, and must click on play button when you see it,
The document describes the market potential of quaternary ammonium compounds. Its application as disinfectant for various types of virus, bacteria, fungus and algae. It explains the preparation of benzalkonium chloride, cetyl pyridinium chloride, tallow alkyl dimethyl ammonium chloride,etc. It also explains the formulations as home care,hand wash, wipes, etc..
Similar to DOC4 - CSPI - Caramel IV Petition to FDA (17)
La industria procesadora de alimentos y la industria química ha dicho por décadas que todos los aditivos utilizados en alimentos han sido probados y que no existe riesgo de utilizarlos. Sin embargo, la historia de los aditivos en los alimentos está llena de casos en que, después de muchos años de ser utilizados, se encontró que realmente sí tenían impactos en la salud. Los aditivos enlistados a continuación han sido prohibidos, después de que las autoridades internacionales y nacionales aseguraron a los consumidores que estos eran totalmente seguros. La moraleja de la historia es que cuando alguien diga que todos los aditivos de alimentos fueron muy bien probados y no tienen riesgo alguno, se deben tomar estas aseveraciones con muchas reservas. Fuente: Center for Science in the Public Interest (CSPI).
Colorantes en alimentos y bebidas que alteran la conducta infantilToxiColaOrg
Desde los años 70´s el pediatra Benjamin Feingold aseguró que existía una relación entre los aditivos y la manera en la que se comportaban los pequeños. Cuarenta años después, el gobierno del Reino Unido, después de una serie de estudios, ha reconocido que cinco colorantes (tartrazina, carmoisina, rojo allura, amarillo ocaso, rojo ponceau 4R) de uso muy común, generan hiperactividad y déficit de atención en los niños. Un estudio realizado en América Latina encontró que la mayor parte de los ´productos comercializados para niños y niñas (bebidas, botanas, pastelillos, postres y yogurts) contienen uno, dos y hasta tres de estos colorantes artificiales.
Estos colorantes pueden provocar problemas de aprendizaje en momentos claves del desarrollo de los individuos, pueden generar tensiones en la relación entre maestros y alumnos, padres e hijos. A pesar de las pruebas, los intereses de la industria procesadora de alimentos han impedido que se prohíba su uso a escala internacional. En este caso, como en muchos otros, como el del colorante caramelo IV, pueden pasar años hasta que sea retirado del mercado, con las consecuencias que para la salud significa su permanencia en el mercado.
La información entre los consumidores es fundamental para evitar el consumo de los productos que contienen estos colorantes. Una vez que las empresas ven que esta información circula entre los consumidores y que puede afectar su imagen y ventas, los retiran de sus productos.
Para proteger a sus hijos y a usted lea este artículo.
DOC2 - No significant risk levels 4 MI - CaliforniaToxiColaOrg
Esta es la propuesta que está presentando la Oficina de Riesgos Ambientales en la Salud de la Agencia de Protección Ambiental de California para establecer un nivel máximo de ingesta diaria de 4-Metilidimadazol (compuesto cancerígeno que se encuentra en el Caramelo IV utilizado en las bebidas de Cola) que no signifique riesgo a la salud. La propuesta de la autoridad en California es que no se consuma más de 16 microgramos de este compuesto en todo un día. Como se ve en el DOC3, la cantidad de 4-Metilidimadazol que se encuentra en una lata de bebida de cola puede ser 8 veces más de este límite.
Esta es una carta elaborada por expertos en toxicología que respaldaron la solicitud presentada por el Centro por la Ciencia de Interés Público (Center for Science in the Public Interest) ante la Administración de Alimentos y Medicamentos (Food and Drug Administration) de los Estados Unidos para que se prohíba el uso del colorante Caramelo IV por contener el compuesto cancerígeno 4-Metilidimazol (ver DOC4). Los científicos advierten el riesgo que significa el consumo del colorante Caramelo IV presente en las bebidas de Cola por contener un compuesto cancerígeno.
DOC1 - National Toxicology Program - Toxicology and Carcinogenesis 4 MIToxiColaOrg
Este es el único estudio realizado en seres vivos para evaluar el potencial toxicológico y cancerígeno del compuesto 4-Metilidimadazol que se encuentra en el colorante Caramelo IV usado por Coca Cola, las bebidas de Cola y otros productos. Fue realizado por el Programa Nacional de Toxicología del Servicio de Salud Pública de los Estados Unidos en 2007. Para evaluar el riesgo que pueden presentar para la salud humana diversos compuestos, se realizan estudios en animales, considerando que si hay un efecto negativo en su salud, estos compuestos no deberían ser consumidos por las personas. El estudio se realizó en ratas y ratones durante dos años y su conclusión fue: "Concluimos que el 4-Metilidimadazol causa cáncer de pulmón en ratones machos y hembras. El 4-Metilidimazol se puede asociar también con el desarrollo de leucemia en ratas hembras".
CDSCO and Phamacovigilance {Regulatory body in India}NEHA GUPTA
The Central Drugs Standard Control Organization (CDSCO) is India's national regulatory body for pharmaceuticals and medical devices. Operating under the Directorate General of Health Services, Ministry of Health & Family Welfare, Government of India, the CDSCO is responsible for approving new drugs, conducting clinical trials, setting standards for drugs, controlling the quality of imported drugs, and coordinating the activities of State Drug Control Organizations by providing expert advice.
Pharmacovigilance, on the other hand, is the science and activities related to the detection, assessment, understanding, and prevention of adverse effects or any other drug-related problems. The primary aim of pharmacovigilance is to ensure the safety and efficacy of medicines, thereby protecting public health.
In India, pharmacovigilance activities are monitored by the Pharmacovigilance Programme of India (PvPI), which works closely with CDSCO to collect, analyze, and act upon data regarding adverse drug reactions (ADRs). Together, they play a critical role in ensuring that the benefits of drugs outweigh their risks, maintaining high standards of patient safety, and promoting the rational use of medicines.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
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DOC4 - CSPI - Caramel IV Petition to FDA
1. UNITED STATES DEPARTMENT OF HEALTH AND HUMAN SERVICES
FOOD AND DRUG ADMINISTRATION
Petition to Bar the Use of Caramel Colorings
Produced With Ammonia and Containing the
Carcinogens 2-Methylimidazole and
4-Methylimidazole.
Submitted by the
CENTER FOR SCIENCE IN THE PUBLIC INTEREST
February 16, 2011
Michael F. Jacobson, Ph.D.
Executive Director
Suite 300
1220 L Street, N.W.
Washington, D.C. 20005
202-332-9110
2. Page 1
February 16, 2011
Ms. Karen Kennard
Division of Dockets Management
Food and Drug Administration
5630 Fishers Lane
Room l061, HFA-305
Rockville, MD 10852
CITIZEN PETITION
The undersigned submits this petition under the Federal Food, Drug, and Cosmetic Act or
the Public Health Service Act or any other statutory provision for which authority has
been delegated to the Commissioner of Food and Drugs under 21 CFR 5.10 to request the
Commissioner of Food and Drugs to revoke sections 21 CFR 73.85 and 21 CFR
182.1235 (generally recognized as safe or “GRAS” regulation), which authorize the use
in foods of caramel colorings that are produced by means of an ammonia or ammonia-
sulfite process and contain 2-methylimidazole and 4-methylimidazole, both of which are
carcinogenic in animal studies. In addition, the FDA immediately should change the
name “caramel coloring” to “chemically modified caramel coloring” or “ammonia-sulfite
process caramel coloring” (and similar terms for other classes of the colorings) and
should not allow products to be labeled “natural” if they contained any type of caramel
coloring.
A. Action Requested
This petition requests the Commissioner to revoke regulations authorizing the use of
caramel coloring in foods. 1
21 CFR 73.85 – Caramel. See Appendix for this regulation.
21 CFR 182.1235 - Caramel.
(a) Product. Caramel.
(b) Conditions of use. This substance is generally recognized as safe when used in
accordance with good manufacturing practice.
As Congress recognized when it passed the food and color additives amendments 50
years ago, chemicals that cause cancer in animals have no place in the food supply. That
is particularly true when the chemicals serve only a cosmetic function, as opposed to a
nutritional or preservative function. Barring the use of the caramel colorings produced
with ammonia could prevent cancer in thousands of consumers. In addition, the FDA
immediately should change the name “caramel coloring” to “chemically modified
caramel coloring” or “ammonia-sulfite process caramel coloring” (and similar terms for
other classes of the colorings) and should not allow products to be labeled “natural” if
they contained any type of caramel coloring.
1
21 CFR 10.30.
3. Page 2
B. Statement of Grounds
I. Production and Use of Caramel Colorings
Caramel coloring is a color additive 2 that is a dark-brown liquid or solid material
resulting from the controlled heat treatment, often under pressure and at high
temperature, of various food-grade carbohydrates, such as high-dextrose corn syrup.
According to one manufacturer, caramel coloring is the world’s most widely consumed
(by weight) food coloring ingredient and that company’s “wide array of natural colorings,
along with our sought after caramel color and burnt sugar, helps sell 1.5 billion servings
of foods and beverages every day.” 3 FDA regulations state, inter alia, “Caramel may be
safely used for coloring foods generally, in amounts consistent with good manufacturing
practice…” No limits are set on the amounts of caramel coloring used in various foods,
though limits are set on the coloring’s lead, arsenic, and mercury content. 4 Limits are not
set on any other components of the coloring. Caramel coloring may be listed on labels
simply as caramel color or color added (its presence would not be indicated on labels of
alcoholic beverages). Importantly, caramel coloring is a cosmetic food additive used to
darken products and does not have any nutritional or preservative function.
Caramel colorings are manufactured in several ways. Caramel Color I (may be used in
high-proof alcoholic beverages) and Caramel Color II (may be used in cognac, sherry,
and vegetable extract) are produced with alkali or acid caustic chemicals and sometimes
sulfites (Caramel II), but not with ammonium compounds. 5 Caramel Color III
(synonyms: ammonia caramel, ammonia process caramel, closed-pan ammonia process
caramel, open-pan ammonia process caramel, bakers’ caramel, confectioners’ caramel,
and beer caramel) is prepared by the controlled heat treatment of carbohydrates with
ammonium compounds. Caramel Color III is often used in baking, beer, soy sauce,
gravy, and other products. Caramel Color IV (synonyms: ammonia sulfite process
caramel, sulfite ammonia caramel, sulfite ammonia process caramel, acid-proof caramel,
beverage caramel, and soft-drink caramel) is prepared by the controlled heat treatment of
carbohydrates with ammonium-containing and sulfite-containing compounds. 6 Soft
drinks colored with caramel coloring generally are made with Caramel Color IV, which is
generally used in colas and certain other soft drinks, and it also may be used in blended
whiskey and general food applications. 7 A key difference between Caramel III and
Caramel IV is that Caramel III carries a positive ionic charge, while Caramel IV carries a
negative charge. That difference renders them appropriate for use in different categories
of foods. 8
2
The American Beverage Association states that caramel coloring is a “Coloring agent with a minimal
effect on the soda flavor.” http://www.ameribev.org/minisites/products/ (accessed Feb. 14, 2011).
3
D.D. Williamson. http://www.caramel.com/ (accessed Feb. 1, 2011).
4
21 CFR 73.85. 21 CFR 182.1235, the GRAS regulation, does not stipulate any limits on any contaminants
or by-products of the manufacture of caramel.
5
http://www.caramel.com/154-Product-Specifications.aspx (accessed Feb. 1, 2011).
6
http://www.inchem.org/documents/jecfa/jecmono/v20je11.htm (accessed Jan. 24, 2011).
7
http://en.wikipedia.org/wiki/Caramel_color (accessed Jan. 24, 2011). http://www.caramel.com/154-
Product-Specifications.aspx (accessed Feb. 1, 2011).
8
Sethness Caramel Color. http://www.sethness.com/dsp_faq.cfm (accessed Feb. 3, 2011).
4. Page 3
Judging from information on a manufacturer’s website, caramel coloring may be used at
a level of about 0.4% in cola beverages. 9 That translates into 1,440 mg per 360 milliliters
(12 oz.). Manufacturers provide information on which caramel-coloring formulations
function best in which foods and beverages. 10
Maillard reactions that occur when carbohydrates and ammonia (with or without sulfites)
are used to produce forms of caramel coloring lead to the formation of numerous by-
products. Two of those by-products are 2- and 4-methylimidazole (“2-MI” and “4-MI”
or “4-MEI”). Those chemicals are widely used in the manufacture of various industrial
chemicals and products. Analytical evidence suggests the presence of 4-MI in the range
of 50–700 parts per million (“ppm”) in caramel colorings, depending upon the process of
manufacture; 200 ppm has been taken as an average low value for 4-MI content.11 We
are not aware of studies that have determined the concentrations of 2-MI in caramel
colorings or in foods. The FDA does not limit the amount of 2-MI or 4-MI in caramel
colorings or in foods.
II. Amounts of 4-Methylimidazole in Beverages
According to the National Toxicology Program (NTP), a division of the National Institute
of Environmental Health Sciences (NIEHS), 2-MI and 4-MI have been identified as
undesirable by-products in several food products, including caramel coloring, soy sauce,
Worcestershire sauce, wine, ammoniated molasses, and caramel-colored syrups.
However, only caramel colors (caramel colors III and IV) manufactured with ammonia or
its salts contain measurable levels of 2- or 4-MI. Those substances have also been
detected in mainstream and sidestream cigarette smoke. 12
In one recent study, researchers at the University of California, Davis, found 4-MI at
levels of 0.30 to 0.36 micrograms/milliliter (ug/ml) in representative brands of colas that
we presume included the two major U.S. brands, Coca-Cola and Pepsi-Cola. A 12-ounce
serving of those drinks would contain 108 to 130 ug of the contaminant. 13,14
9
Technical Tips. D.D. Williamson Co. http://www.caramel.com/EditorUpload/File/Soft-Drinks-Tips-07-
10.pdf (accessed Jan. 24, 2011).
10
D.D. Williamson. http://www.caramel.com/175-Applications.aspx#cooked (accessed Feb. 1, 2011).
Cargill. http://www.cargill.com/food/lat/en/products/caramel-color/darkan-brand-caramel-
coatings/index.jsp.
11
http://www.inchem.org/documents/jecfa/jecmono/v06je13.htm (accessed Jan. 24, 2011). The National
Academy of Sciences’ 2003 Food Chemicals Codex limits 4-MI in caramel to 0.25%, or 250 ppm.
http://www.caramel.com/a-155-286-Food-Chemicals-Codex.aspx (accessed Feb. 1, 2011). The Joint Expert
Committee on Food Additives of the FAO/WHO restricts 4-MI to a “max 300 mg/kg & max 200 mg/kg on
an equivalent color basis” in Class III caramel and “max 1000 mg/kg & max 250 mg/kg on an equivalent
color basis” in Class IV caramel. http://www.fao.org/ag/agn/jecfa-additives/specs/Monograph1/Additive-
102.pdf (accessed Feb. 1, 2011).
12
NTP. Toxicity Report Series Number 67. NTP Technical Report on the Toxicity Studies of
2- and 4-Methylimidazole. NIH Publication No. 04-4409. (April 2004)
http://ntp.niehs.nih.gov/ntp/htdocs/ST_rpts/tox067.pdf (accessed Jan. 24, 2011). (citations not included
here for the references indicated)
13
Moon J-K, Shibamoto T. Formation of carcinogenic 4(5)-methylimidazole in Maillard reaction systems.
J Agric Food Chem. 2011;59:615-8.
5. Page 4
III. Constituents of Caramel-Coloring Production Cause Cancer in Laboratory
Animals
2- and 4-MI have long been known to be toxic to animals. In the past decade, the NTP
tested those two substances in caramel colorings for possible carcinogenicity and other
toxic effects.
(a) 4-Methylimidazole is a Carcinogen in Animals
The NTP conducted short-term feeding studies to establish exposure levels for
subsequent long-term carcinogenicity studies. 15
Mice
The NTP conducted a long-term feeding study (106 weeks long beginning with
mice six weeks of age old) using 4-MI in male and female B6C3F1 mice. 16 As
shown in the Table below, 17 in males, the high-dose group had a significant
increase in the combined incidences of alveolar/bronchiolar adenoma or
carcinoma (p < 0.01). In females, the combined incidences of
alveolar/bronchiolar adenoma or carcinoma were significantly increased in the
mid- (p < 0.001) and high-dose (p < 0.01) groups. In addition, the incidence of
alveolar epithelial hyperplasia was significantly increased in high-dose females.
The tumor incidence in treated females and males exceeded the NTP historical
control incidence for combined alveolar/bronchiolar carcinoma or adenoma.
The NTP concluded: “There was clear evidence of carcinogenic activity of
4-methylimidazole in male and female B6C3F1 mice based on increased
incidences of alveolar/bronchiolar neoplasms.” 18
14
A study from Portugal found as much as 613 ug/L in colas (220 ug/12 fl. oz). (Dark beers ranged from 3
to 424 ug/L.) The authors estimated that “consumer exposure to the maximum 4-MeI given by the soft
drinks was … 5.7 ug/kg body weight/day, in …the United States….” So a 60-kg person would be
consuming 342 ug/day (ignoring other sources of the chemical). Cunha SC, Barrado AI, Faria MA, et al.
Assessment of 4-(5-)methylimidazole in soft drinks and dark beer. J Food Composition and Analysis; 2010.
doi: 10.1016/j.jfca.2010.08.009
15
Chan PC. NTP technical report on the toxicity studies of 2- and 4-Methylimidazole (CAS No. 693-98-1
and 822-36-6) administered in feed to F344/N rats and B6C3F1 mice. Toxic Rep Ser. 2004 Apr;(67):1-
G12. http://ntp.niehs.nih.gov/ntp/htdocs/ST_rpts/tox067.pdf (accessed Feb. 16, 2011)
16
Chan PC, Hills GD, Kissling GE. Toxicity and carcinogenicity studies of 4-methylimidazole in F344/N
rats and B6C3F1 mice. Arch Toxicol. 2008;82(1):45-53.
17
http://www.oehha.ca.gov/prop65/law/pdf_zip/010711NSRLrisk4EI.pdf (accessed Feb. 14, 2011).
18
NTP Toxicology and Carcinogenesis Studies of 4-Methylimidazole (CAS No. 822-36-6) in F344/N Rats
and B6C3F1 Mice (Feed Studies). Natl Toxicol Program Tech Rep Ser. 2007 Jan;(535):1-274.
http://ntp.niehs.nih.gov/index.cfm?objectid=9B956B07-F1F6-975E-79BBCDCCD57001C8
6. Page 5
Table. Incidence of alveolar/bronchiolar tumors in male and female B6C3F1 mice
exposed to 4-methylimidazole via feed for 106 weeks (NTP, Chan, et al., 2008).
Alveolar/
bronchiolar
Sex, Average adenoma or
strain, Concentration daily dose carcinoma Statistical
species in feed (ppm) (mg/kg-day) (combined) significance
Male 0 0 9/50 (18%) NS
B6C3F1 312 40 13/50 (26%) NS
Mice 625 80 16/50 (32%) NS
1250 170 22/50 (44%) p < 0.01
Female 0 0 3/50 (6%) NS
B6C3F1 312 40 8/50 (16%) NS
Mice 625 80 17/50 (34%) p < 0.001
1250 170 14/50 (28%) p < 0.01
Rats
Regarding the two-year feeding study of rats, the NTP concluded that “The
incidence of mononuclear cell leukemia in 5,000 ppm females was significantly
greater than that in the controls, and the incidence slightly exceeded the historical
range in feed study controls.” 19 In addition, significant increases were observed
in chronic focal inflammation of the lung, cardiomyopathy, focal atrophy of the
pancreas acinus, and follicle mineralization of the thyroid gland.
Male rats did not experience significant increases in tumor rates, though chronic
inflammation of the prostate gland, and hypertrophy of the pituitary gland were
seen.
In addition, abnormal behaviors, including hyperactivity, excitability, and
impaired gait were seen in female rats, but not males, at some or all dosage levels
in a dose-dependent way. Whether 4-MI is neurotoxic at the much lower doses to
which humans are exposed is not known.
19
NTP Toxicology and Carcinogenesis Studies of 4-Methylimidazole (CAS No. 822-36-6) in F344/N Rats
and B6C3F1 Mice (Feed Studies). Natl Toxicol Program Tech Rep Ser. 2007 Jan;(535):1-274.
http://ntp.niehs.nih.gov/index.cfm?objectid=9B956B07-F1F6-975E-79BBCDCCD57001C8
7. Page 6
(b) Cola and Other Beverages Contain Unsafe Levels of 4-Methylimidazole
The State of California has concluded that 4-MI is a carcinogen, and is in the midst of a
regulatory proceeding that may require that food and non-food products containing
significant amounts of that chemical bear a cancer-warning notice. California has
determined that levels above 16 micrograms per day pose a significant risk. 20 That No
Significant Risk Level (“NSRL”) is defined as the level of exposure to the chemical that
is calculated to result in no more than one excess case of cancer in an exposed population
of 100,000, assuming exposure over a 70-year lifetime (10-5 lifetime risk of cancer). 21
The roughly 130 ug of 4-MI in a 12-ounce cola is 8 times higher than the NSRL. (Total
production of soft drinks is about 16 ounces per day per capita, with caramel-colored
drinks, such as Coca-Cola, Pepsi-Cola, and Dr Pepper, constituting more than half of
that. 22 ) We estimate average American per-capita consumption of carbonated soft drinks
(including diet drinks) to be about 14 ounces per day. But some population subgroups
consume far more soft drinks than the average person. For instance, the federal
government’s National Health and Nutrition Examination Survey (“NHANES”) 2005–
2006 found that males 14 to 30 years of age consume an average of about two 12-ounce
drinks per day. 23 Colas are the beverages of choice for many of those young men. If the
mean intake were calculated just on the basis of males who consume soft drinks, the
intake would be even higher. An analysis of NHANES 1999–2002 data found that the
90th percentile of 13- to 18-year-old male sugary-drinks consumers imbibed more than
five 12-ounce drinks per day. 24 Those adolescents who drank only colas would have
consumed 650 ug of 4-MI per day, an amount 41 times higher than California’s NSRL.
Additional 4-MI comes from the ammonia- or ammonia-sulfite caramel in beer, soy
sauce, candy, broths, puddings, gravies, and other foods. Moreover, risks from a related
carcinogen, 2-MI, add to the cancer risk from foods and beverages that contain 4-MI (see
next section). These imidazole-containing colorings may be causing hundreds or
thousands of cancers in the American population.
20
The state determined that the NSRL for 4-methylimidazole is 16 micrograms/day. (We recognize that
the FDA extrapolates risks of carcinogens from animals to humans differently from California (and the
Environmental Protection Agency); California’s extrapolation is based on body surface area, not body
weight.)
http://www.oehha.ca.gov/prop65/law/pdf_zip/010711_4MEInotice.pdf (accessed Jan. 24, 2011).
21
http://www.oehha.ca.gov/prop65/law/pdf_zip/010711_4MEIISOR.pdf (accessed Jan. 24, 2011).
22
Beverage Digest. Top-10 CSD companies and brands for 2009. March 24, 2010. http://beverage-
digest.com/pdf/top-10_2010.pdf (accessed Feb. 15, 2011).
23
U.S. Department of Agriculture. What We Eat in America, NHANES, 2005-2006.
http://www.cnpp.usda.gov/Publications/DietaryGuidelines/2010/Meeting6/AdditionalResources/EnergyFro
mSugarSweetenedBeverages.pdf (accessed February 1, 2011).
24
Jacobson MF. Center for Science in the Public Interest. Liquid Candy. Supplement,
Soft Drink Consumption: 1999–2002.
http://cspinet.org/new/pdf/liquid_candy_final_w_new_supplement.pdf (accessed Jan. 31, 2011)
8. Page 7
(c) 2-Methylimidazole is a Carcinogen in Animals
A two-year feeding study conducted by the NTP “demonstrated that 2-MI is carcinogenic
in male and female rats and mice, inducing thyroid follicular cell and hepatocellular
tumors,” as well as other lesions. 25 The results include the following:
Rats
o Females had statistically significant increases in thyroid follicular cell
hyperplasia in all three dosage groups and thyroid follicular cell
adenomas/carcinomas (combined) in the high-dosage group.
o Males (high dose) had an increase in thyroid follicular cell
adenoma/carcinoma (combined) above historical controls, but the
increase was not statistically significant.
o Females and males (mid- and high-dose groups in both sexes) had
rates of hepatocellular adenoma/carcinoma (combined) slightly outside
the range of historical controls, but the increases did not reach
statistical significance.
Mice
o Females in the mid- and high-dose groups had statistically significant
increases in hyperplasia and hypertrophy of thyroid follicular cells, but
not tumors. Females also had a significantly higher incidence of bile
duct hyperplasia and a non-significant increased incidence of
hepatocellular adenoma that was outside the range of historical
controls.
o Males in the high-dose group had statistically significant increases in
thyroid follicular cell adenomas, as well as hyperplasia and
hypertrophy. Males also had a higher rate of combined hepatocellular
adenomas or carcinomas, but the increase did not reach statistical
significance.
The NTP concluded:
There was clear evidence of carcinogenic activity of 2-methylimidazole in
female 344/N rats based on increased incidences of thyroid gland follicular cell
neoplasms. The increased incidences of hepatocellular adenoma in females may
have been related to exposure. There was some evidence of carcinogenic activity
in male B6C3F1 mice based on increased incidences of thyroid gland follicular
cell adenoma and hepatocellular neoplasms. There was some evidence of
25
Chan PC, Sills RC, Kissling GE, et al. Induction of thyroid and liver tumors by chronic exposure to 2-
methylimidazole in F344/N rats and B6C3F1 mice. Arch Toxicol. 2008 Jun;82(6):399-412.
9. Page 8
carcinogenic activity in female B6C3F1 mice based on increased incidences of
hepatocellular adenoma. 26
Thus, the harm to consumers from 4-MI in products made with ammonia (with or without
sulfites) caramel colorings is exacerbated by the 2-MI in those colorings.
(d) Limitations of the NTP Study Protocol
The NTP studies were done according to standard NTP protocol, but that protocol suffers
from at least two weaknesses that reduce the ability to detect carcinogenesis. First, the
studies did not expose the animals in utero, but only after the animals were several weeks
old. Developing fetuses and infants might be especially sensitive to chemical
carcinogens. The FDA recommends that food additives be tested in multi-generation
studies so that the animals are exposed in utero. 27 Second, the studies were ended after
about 106 weeks. Between 62 and 86 percent of the rats and 80 and 92 percent of the
mice treated with 4-MI were still alive at 106 weeks of treatment. Between 56 and 84
percent of the rats and 72 and 92 percent of the mice treated with 2-MI were still alive
after 106 weeks of treatment. Stopping a rodent study and assessing the effects of
chemicals after about two years is roughly equivalent to considering the impact of
chemicals in humans in their 60s. Extending the tests for the entire or almost the entire
lifespan of the animals would give greater opportunity for tumors to develop. 28 While
more spontaneous tumors also would develop, the control group is there to serve as a
benchmark.
IV. 4-Methylimidazole Likely Does Not Prevent Cancer in Rats
As noted above, in recognition of the NTP research on the carcinogenicity of the two
imidazoles, the Office of Environmental Health Hazard Assessment (OEHHA) of the
California Environmental Protection Agency is considering listing 4-MI as a carcinogen
under the Safe Drinking Water and Toxic Enforcement Act of 1986 (“Proposition 65”).29
The food industry is opposing that listing (which would result in warning labels on
various products the consumption of which would lead to ingestion of 16 ug or more of
MI per day) on the grounds that in female and male rats 4-MI had anti-carcinogenic
activity. 30 In all exposed groups of females, the incidences of clitoral gland adenoma,
26
NTP. Toxicology and carcinogenesis studies of 2-methylimidazole (CAS No. 693-98-1) in F344/N rats
and B6C3F1 mice (feed studies). NTP TR 516. NIH Publication No. 05-4456.
http://ntp.niehs.nih.gov/files/516final_web.pdf (accessed Feb. 8, 2011).
27
U. S. FDA. Redbook 2000: IV.C.8 In-Utero Exposure Phase for Addition to Carcinogenicity Studies or
Chronic Toxicity Studies with Rodents. July 2007.
http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/FoodIngredien
tsandPackaging/Redbook/ucm078394.htm (accessed Feb. 1, 2011).
28
Huff J, Jacobson MF, Davis DL. The limits of two-year bioassay exposure regimens for identifying
chemical carcinogens. Environ Health Perspect. 2008 Nov;116(11):1439-42.
29
http://oehha.ca.gov/prop65/crnr_notices/admin_listing/intent_to_list/noilpkg32.html and
http://oehha.ca.gov/prop65/prop65_list/newlist.html (accessed Feb. 2, 2011).
30
Chan PC, Hills GD, Kissling GE. Toxicity and carcinogenicity studies of 4-methylimidazole in F344/N
rats and B6C3F1 mice. Arch Toxicol. 2008;82(1):45-53.
10. Page 9
mammary gland fibroadenoma, and uterine stromal polyp were significantly less than
those in the control group. In 4-MI-treated male rats, decreased incidences of neoplasm
were observed in the adrenal medulla and pituitary gland (pars distalis). (In mice, 4-MI
was a clear-cut carcinogen in males and females, and had no significant anti-carcinogenic
activity.)
A law firm, Morrison–Foerster, representing a food industry coalition has vigorously
opposed OEHHA’s proceeding to list 4-MI as a carcinogen. In a 2009 letter, Morrison–
Foerster told OEHHA that “The evidence that 4-MEI prevents tumors in rats is
significantly stronger than the evidence that 4-MEI causes tumors in mice.” 31 F.J.
Murray, one of the authors of the 2009 Morrison–Foerster submission, acknowledged in a
separate article (sponsored by the American Beverage Association) that “Reduced body
weight offers a partial explanation for the reduction in tumors.” 32 [emphasis added]
However, lower body weight might account for most or even all of the reduction. The
NTP study found that the body weights of the dosed female rats averaged as much as 35
percent less than the controls and that the lower tumor incidences “were probably related
to loss of body weight resulting from exposure concentration-related body weight loss.”
It is widely accepted that reduced body weight, as in diet-restricted animals (including
humans), tends to reduce incidences of cancer. Haseman, et al., used statistical analysis
to show that “Incidences of certain site-specific tumors, most notably mammary gland
and pituitary gland tumors in rats and liver tumors in mice, were shown to have a strong
positive correlation with 52-wk body weight.” 33 Murray et al., asserted that that paper
indicated that reduced body weight likely was not the sole factor in the reduced tumor
incidences. Importantly, though, Haseman, et al., assessed relationships between body
weight and tumor incidence in untreated animals, which could be very different from
animals treated with a chemical that is carcinogenic in mice and possibly rats. In
addition, some of the 4-MI-consuming rats had body weights outside the range of the
rats’ weights in the Haseman, et al., study.
Furthermore, most of the organs that had reduced tumor incidences in rats are hormone-
sensitive organs, suggesting an endocrine-related effect. Nothing is known about the
mechanism that led to fewer tumors in 4-MI-treated rats, but it is possible that the high
doses used in the study affected hormone levels, which then led to the reduced incidences
of tumors. Notwithstanding lack of information about the mechanism of cancer
prevention (if that is due to something other than low body weight), it is possible, and
perhaps likely, that even if high doses of the chemical prevent cancer in rats, low doses
would not. In contrast, chemicals that cause cancer in laboratory animals at high dosages
31
Murray FJ, Corash MB. Letter to California’s Office of Environmental Health Hazard Assessment. Sept.
1, 2009.
A submission by MB Corash at the same consulting firm on May 1, 2008, said that the firm was
representing a coalition that included the Grocery Manufacturers of America, California Grocers
Association, American Frozen Food Institute, American Beverage Association, and National Confectioners
Association.
32
Murray FJ. Does 4-methylimidazole have tumor preventive activity in the rat? Food Chem Toxicol.
2011;49:320-2.
33
Haseman JK,Young E, Eustis SL, et al. Body weight-tumor incidence correlations in longterm rodent
carcinogenicity studies. Toxicologic Pathology. 1997;25(3):256-63.
11. Page 10
are considered, absent evidence to the contrary, to also cause cancer at the much lower
dosages to which humans are exposed. Cancer expert Ron Melnick, formerly of the NTP
and now an NIEHS retired associate, says, “Not knowing the mechanism(s) for the
decreased tumor incidences by 4-MI is a major weakness to the conclusion that ‘the
evidence that 4-MEI prevents tumors in rats is significantly stronger than the evidence
that 4-MEI causes tumors in mice,’” as industry claimed. 34
Murray suggested that 4-MI might be useful in drug development, stating “4-MEI and
structural analogues may represent a potential lead for cancer prevention research.” 35
But Melnick objects, saying, “It would be irresponsible to use to an agent that causes
lung tumors for possible prevention of human cancer.” 36 Even Murray acknowledged
that the possible cancer-preventative power of 4-MI is quite speculative:
The reasons for the different response to 4-MEI in rats and mice is [sic]
unknown. Further, it is unclear whether humans are more like mice or rats in
terms of their response to 4-MEI. 37 [emphasis added]
OEHHA rejected the food industry’s contentions. 38 It stated that most of the decreases
were in benign, not malignant, tumors, and it questioned the use of the Haseman, et al.,
model. More importantly, though, it stated,
While the observations of decreases in tumor incidences in the NTP rat studies
are scientifically interesting, they do not call into question the findings of the
NTP mouse studies….OEHHA is unaware of guidance used by any authoritative
body regarding the identification of cancer hazards that allows evidence of
decreased tumor incidences in one species of experimental animals to weigh
against increased incidences in another species.
OEHHA also chided Murray and Corash for not having “cited any authority supporting
their suggested approach to hazard identification [of balancing decreases in tumor
incidences in one species against increases in another].”
It would be irresponsible to permit continued uncontrolled human exposure via the food
supply to a known animal carcinogen in the hope that it would prevent far more cancers
than it causes. Dale Hattis, a research scientist at Clark University specializing in issues
of quantitative risk assessment, including both cancer and non-cancer effects,
emphasizes, “It would, of course, set a very dangerous precedent for 4-MI to be given a
pass on its carcinogenic activity on the basis of high-dose toxicity-related tumor
suppression with highly uncertain implications for dose-response and inter-species
projections.” 39
34
Melnick R. Pers. Comm. Feb. 1, 2011.
35
Murray FJ. Op cit.
36
Melnick R. Op cit.
37
Murray FJ. Op cit.
38
Alexeeff GV. OEHHA. Calif. EPA. Dec. 31, 2010. Letter sent to Murray FJ and Corash MB, Morrison
and Foerster.
39
Pers. Comm. Jan. 31, 2011.
12. Page 11
In sum, the evidence demands, and prudence would dictate, that 4-MI’s carcinogenicity
not be excused by the unsubstantiated argument that the chemical is a cancer-preventing
agent.
V. FDA Should Stop the Deceptive Labeling of Caramel-colored Products
Some companies market caramel-colored products as “natural” foods (for example,
Whole Foods Markets’ “natural” 365-brand cola). Considering that all caramel colorings
are highly processed, heat- and sometimes-pressure-treated carbohydrates, possibly
reacted with strong acids, bases, ammonia, and/or sulfites, the FDA should not allow
“natural” claims on foods and beverages containing any type of caramel coloring.
Also, even the term “caramel coloring” can be misleading, because it conjures up images
of home-made caramel. The FDA immediately should change the names of all classes of
caramel coloring to “chemically modified caramel coloring” or “ammonia-sulfite [or acid
or alkali or sulfite or ammonia] process caramel coloring” (or similar terms for other
classes of the colorings). Such nomenclature would be analogous to “chemically
modified starch” or “cocoa (processed with alkali).”
VI. FDA has the Authority and an Obligation to Protect Consumers from
Ammonia- and Ammonia-Sulfite-Process Caramel Colorings that contain 2-
Methylimidazole and 4-Methylimidazole.
Section 721(b)(3) of the Federal Food, Drug, and Cosmetic Act (“FFDCA”), 21 U.S.C.
379e(b)(3), provides that in order “to assure the safety of the use” of a color additive the
FDA “shall...prescribe the conditions under which such additive may be safely employed
for such use or uses (including, but not limited to...labeling...for such additive).”
Section 721(b)(4) of the FFDCA, 21 U.S.C. 379e(b)(4), bars the use of any color additive
in food 40 unless the FDA has found that “the data...establish that such use, under the
conditions of use specified in the regulations, will be safe.” Section 721(b)(5)(C)(i)
authorizes the “amendment or repeal” of any food color regulation. Section 201(u) of the
FFDCA, 21 U.S.C. 321(u), says that “the term ‘safe,’ as used in... section 721, has
reference to the health of man or animal.”
The FDA’s regulations say that “safe means that there is convincing evidence that
establishes with reasonable certainty that no harm will result from the intended use of the
color additive.” [emphasis added] 21 C.F.R. 70.3(i).
A fair evaluation of all the scientific evidence, as discussed above, makes it clear that the
ammonia- or ammonia-sulfite-process caramel colorings that contain 2-MI and/or 4-MI
and are used in foods and beverages are unsafe within the meaning of the FFDCA and the
FDA’s regulations under the Act. Indeed, instead of there being “convincing evidence”
that the color additive causes “no harm,” there is significant evidence that the coloring
40
The statute also applies to the safety of color additives in drugs, devices, and cosmetics.
13. Page 12
does cause harm considering that the NTP found that the two chemicals caused tumors in
mice and rats. Though one NTP study found that 4-MI reduced the risk of certain tumors
(mostly benign) in female (and to a lesser extent male) rats, there is neither evidence that
the reduction is anything more than a result of reduced body weight nor that the effect,
even if real in rats, occurs at the much lower dietary amounts consumed by humans. And
even if 4-MI did confer a benefit, the FFDCA—nor any authoritative agency concerned
about the carcinogenicity of environmental chemicals—does not contemplate balancing a
color additive’s risks against putative benefits.
Section 721(b)(8), 21 U.S.C. 379e(b)(8), of the FFDCA further provides that in deciding
whether to approve a color additive for all uses the FDA should “take into
account…(subject to the paramount criterion of safety)...the availability, if any, of other
color additives suitable and safe for one or more of the uses proposed.” Inasmuch as
caramel colorings serve only a purely cosmetic purpose, they easily could be omitted
from food and beverage formulations or sometimes be replaced by natural color additives
or ingredients. Indeed, in the early 1990s Coca-Cola Co. marketed Tab Clear and called
it “the ultimate diet soft drink,” while PepsiCo marketed Crystal Pepsi, using the slogan
“You've never seen a taste like this.” 41 Both beverages were water clear. Crystal Pepsi
was reported to have “tasted much like the original Pepsi.” Clearly (pun intended),
reformulating colas without caramel colorings would be simple and might even reduce
ingredient costs for the manufacturers. Knowing the creativity of the food and chemical
industries, other ingredients (such as natural colorings or caramel colorings produced
without ammonia) may be available that could provide the color now conferred by
caramel colorings. In any case, “the paramount criterion of safety” renders the
availability of alternative colorings irrelevant to the FDA’s action on this matter.
The “Delaney clause” of the FFDCA, 21 USC 379e(b)(5)(B), specifically bars the use of
cancer-causing colorings:
A color additive (i) shall be deemed unsafe, and shall not be listed, for any use
which will or may result in ingestion of all or part of such additive, if the additive
is found by the Secretary to induce cancer when ingested by man or animal, or if
it is found by the Secretary, after tests which are appropriate for the evaluation of
the safety of additives for use in food, to induce cancer in man or animal, and (ii)
shall be deemed unsafe, and shall not be listed, for any use which will not result
in ingestion of any part of such additive, if, after tests which are appropriate for
the evaluation of the safety of additives for such use, or after other relevant
exposure of man or animal to such additive, it is found by the Secretary to induce
cancer in man or animal…
Under that standard, the regulations authorizing the use of caramel colorings containing
2- and 4-MI must be revoked because 2- and 4-MI are unavoidable constituents created
when sugar is caramelized in the presence of ammonia and are an integral part of the
41
Bryant A. Company News; Coke Adds a Clear Cola To Its 'New Age' Stable. New York Times. Dec. 15,
1992. http://www.nytimes.com/1992/12/15/business/company-news-coke-adds-a-clear-cola-to-its-new-
age-stable.html (accessed Feb. 1, 2011). Steve. Bevreview.com. Is Pepsi Clear the return of Crystal Pepsi?
http://www.bevreview.com/2009/08/11/pepsi-clear-crystal-pepsi/ (accessed Feb. 1, 2011).
14. Page 13
complex mixture of breakdown and reaction products of sugar that constitutes artificial
caramel colorings.
Some might argue that the FDA should not apply the Delaney clause, but rather the
contaminants exception to the Delaney clause. As the FDA said regarding D&C Black
No. 2,
…where an additive itself has not been shown to cause cancer, but contains a
carcinogenic impurity, the additive is properly evaluated under the general safety
standard using risk assessment procedures to determine whether there is a
reasonable certainty that no harm will result from the intended use of the additive
(Scott v. FDA, 728 F.2d 322 (6th Cir. 1984)). 42
That exemption was intended to avoid situations in which an additive used in small
amount would have to be banned under the Delaney clause because it contained
minuscule amounts of a carcinogen, which would pose a negligible risk to human health.
For instance, the FDA calculated the risk from the carcinogenic contaminant in Black No.
2 to be less than one cancer in one billion people. The case of ammonia- and ammonia-
sulfite-process caramel colorings and 4-MI is starkly different. The coloring–
contaminant mixtures pose a risk to consumers far greater than the FDA’s benchmark of
one cancer in a million people, according to California’s risk assessment. And, as
discussed above, any contention that putative anti-cancer benefits of 4-MI outweigh the
risks should be rejected as unprecedented and speculative.
VII. FDA has the Authority and an Obligation to Protect Consumers from
Deceptively Labeled Caramel-colored Products
Section 721(b)(6) of the FFDCA states that “the Secretary shall not list a color
additive ... for a proposed use if the data before him show that such proposed use would
promote deception of the consumer in violation of this Act or would otherwise result in
misbranding ...within the meaning of this Act.” Section 201(n) of the FFDCA, 21 USC
321(n), provides, in pertinent part, that
in determining whether the labeling...is misleading there shall be taken
into account (among other things) not only representations made or
suggested by statement, word, design, device, or any combination thereof,
but also the extent to which the labeling...fails to reveal facts material in
the light of such representations or material with respect to consequences
which may result from the use of the article to which the labeling...relates
under the conditions of use prescribed in the labeling...thereof or under
such conditions of use as are customary or usual.
42
Federal Register: July 28, 2004 (Vol. 69, No. 144, Page 44927-30).
http://www.fda.gov/OHRMS/DOCKETS/98fr/04-17153.htm (accessed Jan. 25, 2011).
15. Page 14
Thus, the FDA has ample authority to bar deceptive labeling claims, such as calling
products “natural” event though they contain caramel coloring, and to require that
caramel colorings chemically reacted with ammonia or sulfites be called “chemically
modified caramel colorings.”
VIII. Conclusion
As a matter of law, and as an essential measure to protect the public health, the
FDA should ban the use of ammonia- and ammonia-sulfite-process caramel colorings
that contain 2-methylimidazole and 4-methylimidazole, both of which cause cancer in
laboratory animals. Those colorings are used for purely cosmetic purposes and serve no
nutritional or preservative function. The FDA should not delay eliminating dangerous
artificial caramel colorings from the food supply and to require more honest labeling of
any caramel colorings left on the market.
C. Environmental Impact
The action requested is subject to a categorical exclusion under 21 C.F.R. 25.30
and 25.32 and therefore does not require the preparation of an environmental assessment.
D. Economic Impact
No statement of the economic impact of the requested action is presented because
none has been requested by the Commissioner. 43
E. Certification
The undersigned certifies that, to his best knowledge and belief, this petition
includes all information and views on which the petition relies, and it includes
representative data and information known to the petitioner that are unfavorable to the
petition.
Respectfully submitted,
Michael F. Jacobson, Ph.D.,
Executive Director
Center for Science in the Public Interest
1220 L St. NW Suite 300
Washington, DC 20005
202-777-8328
mjacobson@cspinet.org
43
21 C.F.R. 10.30(b).
16. Page 15
Appendix
21 CFR 73.85 - Caramel.
(a) Identity. (1) The color additive caramel is the dark-brown liquid or solid material
resulting from the carefully controlled heat treatment of the following food-grade
carbohydrates: dextrose, invert sugar, lactose, malt sirup, molasses, starch hydrolysates
and fractions thereof, sucrose.
(2) The food-grade acids, alkalis, and salts listed in this subparagraph may be employed
to assist caramelization, in amounts consistent with good manufacturing practice.
(i) Acids: Acetic acid, citric acid, phosphoric acid, sulfuric acid, sulfurous acid
(ii) Alkalis: Ammonium hydroxide, calcium hydroxide U.S.P., potassium hydroxide,
sodium hydroxide.
(iii) Salts: Ammonium, sodium, or potassium carbonate, bicarbonate, phosphate
(including dibasic phosphate and monobasic phosphate), sulfate, and sulfite.
(3) Polyglycerol esters of fatty acids, identified in 172.854 of this chapter, may be used
as antifoaming agents in amounts not greater than that required to produce the intended
effect.
(4) Color additive mixtures for food use made with caramel may contain only diluents
that are suitable and that are listed in this subpart as safe in color additive mixtures for
coloring foods.
(b) Specifications. Caramel shall conform to the following specifications:
Lead (as Pb), not more than 10 parts per million;
Arsenic (as As), not more than 3 parts per million;
Mercury (as Hg), not more than 0.1 part per million.
(c) Uses and restrictions. Caramel may be safely used for coloring foods generally, in
amounts consistent with good manufacturing practice, except that it may not be used to
color foods for which standards of identity have been promulgated under section 401 of
the act unless added color is authorized by such standards.
(d) Labeling. The label of the color additive and any mixtures prepared therefrom and
intended solely or in part for coloring purposes shall conform to the requirements of
70.25 of this chapter.
(e) Exemption from certification. Certification of this color additive is not necessary for
the protection of the public health and therefore batches thereof are exempt from the
certification requirements of section 721(c) of the act.