FOR PG

1. Punyashlok Ahilyadevi Holkar
University Solapur
RESEARCH PROJECT
(Project review)
“A Review on Spectrophotometric determination of Cobalt(II)”
For
PARTIAL FULFILLMENT OF THE REQUIREMENT OF
DEGREE OF MASTER OF SCIENCE IN ANALYTICAL CHEMISTRY
By
Miss. Jadhav Swati Bhagvan
M.Sc. II Sem(III)(Analytical Chemistry)
UNDER THE GUIDANCE OF
Asst. Prof. Dr.U.B.Barache
M. Sc.,B.Ed.,Ph.D.SET
Submitted To
Department of Chemistry
Shri Shivaji Mahavidyalaya, Barshi
2024-25

2.  General Properties of Cobalt

3.  Uses of Cobalt
The most common uses of cobalt are in some of the everyday
materials we use such as: magnets, ceramics, magnetic alloys, cobalt
boats, glassware, steel-boating radial tires and it is also used in
radiotherapy. Cobalt is widely used in batteries and in electroplating.
Cobalt salts are used to impart blue and green colors in glass and
ceramics.

5.  Literature Survey
Sr. No. Reagent Λ max Medium
or pH
Beer's
law
range
Remarks References
1 Ninhydrin 395 nm pH 8.2 - Spectrophotometric
estimation of cobalt
with ninhydrin complex
[1]
2 2-Chloro benzaldehyde
thiocarbohydrazone
(CBTCH)
400 nm pH 4.2 13
μg /mL-1
Analysis of alloy and
synthetic mixture
[2]
3 4-(6-nitro-2-
benzothaiazolylazo)
resorcinol (6-NO2
BTAR)
458 nm pH 6.0 - Successfully applied to the
determination of cobalt
[3]
4 4-(2-Hydroxy naphthalen-
1-ylazo) Nthiazol-2-
yl benzene sulfonamide
390 nm pH 13 - Aqueous solution samples
utlizing a new
chemosensor
[4]
5 5-(4-hydroxy- 3-5-dimethyl
benzylidene) thiazolidine-
2, 4 dione (HDBT)
517 nm pH 5.7-
6.9
- The struture of the
complex was studied by
spectrophotometry, IR
spectroscopy &
thermogravimetry
[5]

6. Sr.
No.
Reagent Λ max Medium or
pH
Beer's law
range
Remarks Reference
s
6 N,N'bis (o-hydroxy
acetophenone) ethylene
diimine (HAPEO)
400 nm pH 2.6 1-10 μg
/mL-1
determination &
separation of Co(II) in
commercial mixture,
Pharmaceutical Sample
& alloys
[6]
7 1-(2,4-dinitro aminophenyl)-
4,4,6- trimethyl-1,4-diny -
dropyrimidine -2-thiol
490 nm pH 9.5-10.2 - comparison of
experimental results
with atomic absorption
spectrophotometer.
[7]
8 5-methylfuran-2
carbaxaldehyde thio
semicarbazone) (5-MFAT)
360 nm pH 6 0.296-2.36
μg /mL-1
comparable with those
from atomic absooption
Spectrometry and were
found to be in good
agreement.
[8]
9 1-(2-pyridylazo)-2 naphthol
(PAN)
645-650
nm
pH 11 - Atomic Adsorption
Spectrophometric
method was achieved
wher the proposed
methodology was
applied for
determination of cobalt.
[9]
10 2-Hydro 5-methyl 3-nitro
Acetophenone Oxime
(HNMA).
420 nm pH 7.0-8.0 - Interference by various
ions has been studied
[10]

7. Sr. No. Reagent Λ max Medium or pH Beer's law
range
Remarks References
11 2-[(2-hydroxyphenylimino
methyl)]-4-nitrophenol
[HPIMNP]
465 nm pH 9-10 - Determination of Co(II) in
alloy and pharmaceutical
samples
[11]
12 2-[di-(2-pyridyl)methyli-
denehydazino]quinoline.
510 nm pH 2-12 - Co (II) has also been
determined in alloys
[12]
13 [2-(4-
methoxyphenyl)azo(4,5-
dipheny imidazole)](MPAI)
491 nm pH 9 - Different parameters such as
effect of pH, concentration
reagent, time, Oxidation-
reduction agent were studied
[13]

8.  Some reactions
• Reaction of ninhydrin with cobalt ion in an alkaline solution[1]

9. Synthesis of 2-CBTCH[2]

10. Structures
Fig :4-(2-hydroxy-naphthalen-1-ylazo)-N-thiazol-
2-yl-benzenesulfonamide.[4]

11. Structure of 2,6-
Pyridinedicarboxaldehyde,thiosemicarbazone[14]

12. • Co(II) element is more usually produced as a byproduct of copper and nickel
mining.
• The spectrophotometric technique was used to determination of Co(II) in water ,
alloy and nano powders.
• Co(II) is stable in aqueous solutions.
• Co(II) is a micronutrient that plays important role in many biological processes.
• The spectrophotometric techniques have been characterized with
physicochemical techniques, elemental evaluation, magnetic moment
measurements and spectroscopic thermogravimetric techniques and X-ray
powder diffraction.
• A spectrophotometric technique has been developed for detection of Co(II) ions
in aqueous solution sample utilizing a new chemosensors.
 Conclusion

13.  [1]M. Karamat ,H.W.Firoza, I.Muhammad, J.A.Muhammad , A.T.Syed Ahmed, W.Abdul,Spectrophotometric
estimation of cobalt with ninhydrin,Saudi Journal of Biological Sciences, 19(2012)247-250.
 [2] A.V. Sadlapurkar, U.B. Barache, A. B. Shaikh, A.S. Lawand, S.H. Gaikwad,, T. N.Lokhande.,Development
of new efficient and cost liquid-liquid extractive determination method of for Cobalt( ): Analysis of water ,
Ⅱ
alloys and nano powder, Journal of trace elements and minerals, 200(2022)100026.
 [3] N. A. Nassir.,Spectrophotometric determination of Cobalt( ) using 4-6-Nitro-2-benzothiazolylazo)
Ⅱ
resorcinol, National journal of chemistry, 26(2007)263-269.
 [4] S. Nassar, G. I. Mohammed, Spectrophotometric determination of cobalt ions in water using by metallic
hemosensor based on sulfa drug azo dye, Journal of umm Al-Qura University for Applied sciences ,024-00167-
5.
 [5] K. R. Alieva, Spectrophotometric determination of cobalt (II) with 5-(4-Hydroxy-3,5- Oi
methylbenzilidene) thaizolidine -2,4, Drones, Joumal of multidisciplinary engineering science and
Fechnology , 8(2021).
 [6] J.S.Patil, R. S. Lokhande , S.B.Dharap, P. P. shevede and S. Patil, Liquid- liquid extraction and
spectrophotometric determination of Co( ) with N,N’ bis Co- hydroxy acetophenone) ethylene diimine
Ⅱ
derivative as an analytical reagents, International Journal of chemistry and Applications, 5(2013)133-139.
 Reference

14.  [7] G. S. Kamble, A. A. Ghare, S. S. Kolekar, S. Han , M. A. Anuse, Development of an reliable analytical method
for synergistic extractive Spectrophotometric determination cobalt (II) from alloys and nano composite sample by
using chromogen chelating ligand, spectrochemica Acta Part A, 84(2011)117-124.
 [8] G. S Reddy, P. R. Reddy, Synthesis of New-s-methylferran-2-carbaxaldehyde thio semicarbazone CS-MFAT):
Selective and Sensitive Spectrophotometric Determination of Co CIL) in Vegetable, Soil, water and Alloy samples,
J. Mater. Environ. Sci., 6(6)(2015) 1699-1708.
 [9]E. k. Paleologos, M. I. Prodromidis, D. I. Giokas, Al.C. Pappas, M. I. Karayannis, Highly selective
spectrophotometeic of trace cobalt and developement of a reagentless fiber- optic sensor, Analytica the chimica
Acta, 467(2002)205-215.
 [10] A. D Sawant, V. D. Barhate, R.S. Lokhande , Extractive spectrophotometric of Cobalt (II) with 3-Nitro 2-
Hydroxy determination 5-methyl Acetophenone Oxime (HNMA), J. Ind. Council Chem. , 27(2010)215-216.
 [11] Datta B. Mandhare, Vasant D. Barhate, “Developement of Extractive Spectrophotometric Method for the
Determination of Cobalt(II) Determination with Schiff base 2-[(2-Hydroxyphrnykimino) methyl] -4-Nitrophenol”,
Journal of chemistry and Chemical Sciences,6(2)(2016)143-148.
 [12] R.B. Singh, H.L. Ray, B.S. Garg, R.P. Singh, Spectrophotometric determination of Co(II) with 2-[(di (2-
pyridyl)- methylidene hydrazino] quinoline, 26(1979)898-900.
 [13] A.F. Hussein, R.S.Hatam, I.M. Shaheed, Spetrophotometric study for Determination of Cobal(II) by the
Reagent [2-(4-Methoxyphenyl)Azo(4,5 - Dipheny Imidazole)] (MPAI),Human Journals, 6(2016).

15.  [14] M. F. Ahmed, Spectrophotometric determination of cobalt in biological and environmental samples using
2,6 Pyridine di carboxaldehyde thiosemicarbazone, International journal of Analytical, Pharmaceutical and
Biomedical Sciences, 2(2013).

16. THANK YOU...