The cholinergic system utilizes acetylcholine as a neurotransmitter. Acetylcholine is synthesized from acetyl CoA and choline by the enzyme choline acetyltransferase and stored in vesicles. When an action potential reaches the presynaptic membrane, acetylcholine is released into the synaptic cleft and binds to cholinergic receptors on the postsynaptic membrane, leading to depolarization. There are two types of cholinergic receptors: muscarinic and nicotinic. Cholinergic drugs act on the same sites as acetylcholine and include esters of choline, cholinomimetic alkaloids, and anticholinesterases which inhibit the enzyme that breaks down acetylcholine.