The document summarizes the process of human fertilization in 6 stages: 1) Capacitation of sperm in the female reproductive tract. 2) The acrosome reaction allows sperm to penetrate the corona radiata. 3) A single sperm fuses with the oocyte plasma membrane. 4) The second meiotic division of the oocyte is completed, forming the female pronucleus. 5) The male and female pronuclei fuse, forming a single cell with a full chromosome complement. 6) The zygote undergoes its first cell division, forming a two-cell embryo.

1. Getting to the right site:
Helping the sperm:
 At ovulation, the cervical mucus increases in amount
and becomes less thick, allowing easier sperm transport.
 Passage of the sperm through the uterus and oviduct
occurs mainly due to muscular contractions of these
organs.
Oocyte:
 The ends of the oviducts come into close contact with
the ovary during ovulation.
 Fimbriae of oviduct ends “sweep” the ovulated ovum into
the oviduct.
 Peristaltic waves of oviduct musculature bring the ovum
into the ampulla of the oviduct.

2. The Oviduct
H & E × 10
M = muscle
BL = broad ligament
S = serosa with vascular
supporting tissue
From Wheater’s Functional Histology, 4th
ed., 2000.

3. Morphology of the Oviduct:
Fallopian tube S = smooth muscle
H & E × 150 E = ciliated epithelium Fallopian tube
Azan × 320From Wheater’s Functional Histology, 4th
ed., 2000.

4. Capacitation: readying the sperm
Sperms cannot fertilize oocytes when they
are newly ejaculated.
The process of capacitation takes 5-7 hours.
Capacitated sperms are more active.
Location: capacitation occurs in the uterus
and oviducts and is facilitated by substances
of the female genital tract.
The acrosomal reaction cannot occur until
capacitation has occurred.

5. Stage 1 of fertilization:
The acrosome reaction must be completed before
the sperm can fuse with the secondary oocyte
 Occurs when sperms come into contact with the corona
radiata of the oocyte
 Perforations develop in the acrosome
 Point fusions of the sperm plasma membrane and the
external acrosomal membrane occur
 The acrosome reaction is associated with the release of
acrosome enzymes that facilitate fertilization
Passage of sperm through the corona radiata
depends on enzyme action:
 hyaluronidase released from sperm acrosome
 Tubal mucosal enzymes
Flagella action also aids corona radiata penetration

6. Ovum and sperms: (In vitro)
Advanced Fertility Center of ChicagoAdvanced Fertility Center of Chicago
http://www.advancedfertility.com/http://www.advancedfertility.com/
The surfaces of unfertilized eggs are usually smooth in appearance. The mottled look of this
egg is not normally seen, but apparently all the ova from this woman had this appearance.
From this photograph, it
should be clear that the heads
of human sperm are less than
1/20 the diameter of human
eggs.
 Arrows point to sperm heads

7. Stage 2 of fertilization:
Penetration of the zona pellucida around the
oocyte:
Acrosomal enzymes: esterases, acrosin, and
neuraminidase cause lysis of the zona pellucida
Once sperm penetrates zona pellucida, the
zona reaction occurs:
This reaction makes the zona pellucida
impermeable to other sperms.
When more than one sperm manages to enter
the ovum (dispermy = 2; triploidy = 3), the fetus
nearly always aborts.

8. Stages 3 & 4 of fertilization:
Fusion of plasma membranes of oocyte and
sperm
Head and tail of a sperm enter the cytoplasm of
the oocyte, but the sperm plasma membrane
remains behind.
2nd
meiotic division of oocyte is completed
The secondary oocyte was previously arrested
in metaphase of the 2nd
meiotic division, and now
forms the mature ovum and another polar body.

9. Stage 5 of fertilization:
Formation of male and female pronuclei:
Chromosomal material of the sperm
decondensates and enlarges
Chromosomal material of the ovum
decondensates following the completion of
meiosis
At this stage, the male and female
pronuclei are indistinguishable.
As they grow, the pronuclei replicate their
DNA  still 1N (haploid)- 23
chromosomes, each in chromatid pairs

10. The male and female
pronuclei are
indistinguishable from one
another.
The second polar body can
be seen (arrow).
The plasma membranes of
the two pronuclei are
dissolving and one diploid
nucleus will remain.
Fusion of the pronuclei:
(in vitro)
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http://www.advancedfertility.com/http://www.advancedfertility.com/

11. Stage 6 of fertilization:
Membranes of the pronuclei break down,
chromosomes condense and arrange
themselves for mitotic cell division
On membrane dissolution, there is 1 cell with
46 chromosomes = diploid (2N)
The first cleavage follows shortly, leaving 2
cells, each with 46 chromosomes.
 Mitosis in the new zygote uses centrioles derived
from the sperm. The oocyte has no centrioles.

12. Fertilization facts:
Completed within 24 hours of ovulation
Approximately 400 to 600 MILLION sperms are
deposited at cervical opening during ejaculation.
 Some sperm are held up by the folds of the cervix and
are gradually released into the cervical canal; this
gradual release increases the chances of fertilization.
 Most human sperms do not survive longer than 48 hours
in the female genital tract.
Only about 200 sperms reach the fertilization site;
most degenerate and are absorbed by the female
genital tract.

13. The results of fertilization:
Stimulates the secondary oocyte to complete
meiosis.
Restores the normal diploid number of
chromosomes (46).
Results in variation of human species as maternal
and paternal chromosomes intermingle.
The embryo contains only maternal mitochondria
because the sperm mitochondria are dispersed into
the egg cytoplasm and discarded.
Determines the sex of the embryo.
 The sex chromosome (Y or X) carried by the successful
sperm determines embryonic sex.

14. Twins: still 1 sperm per egg
Monozygotic (monoovular):
A fertilized, single egg splits into two developing
zygotes at a very early stage.
Identical twins; same sex.
Dizygotic (polyovular):
Result from the fertilization by two sperm of two
separate ova that have reached maturation at
the same time.
Not identical twins; can be different sexes
Incidence increases with age of the mother

15. How can fertilization go awry?
Too many sperm = dispermy or triploidy
Leads to spontaneous abortion in most cases.
Infertility
Bad timing:
The sperm can only survive 48 hours within the
female genital tract.
In vitro studies show the ovulated egg cannot be
fertilized after 24 hours.

16. Triploidy (in vitro)
Advanced Fertility Center of ChicagoAdvanced Fertility Center of Chicago
http://www.advancedfertility.com/http://www.advancedfertility.com/
There are 3 pronuclei
within this one zygote.
In the laboratory, such
embryos are discarded.
In vivo, such embryos
almost always abort
spontaneously.

17. A little more on sex determination:
Not so clear-cut as X and Y:
 The presence of other genes, located on the X, Y and
other chromosomes, influence the determination of
embryonic sex.
The SRY gene:
 Normally present on the Y chromosome
 Induces the sexless embryonic gonad to develop into a
testis
 Can sometimes be present on the X chromosome, leading
a genetic female (XX) to develop testis
 1990: a fertilized mouse embryo with the XX configuration
was injected with SRY and developed as a male both
anatomically and behaviorally.
 (Andrew Sinclair, Center for Hormone Research, University of Melbourne)