This document provides descriptions and proper uses of common laboratory equipment used in chemistry labs. It describes various pieces of glassware like beakers, erlenmeyer flasks, test tubes, and funnels that are used to contain liquids and solids. It also outlines equipment for heating substances like burners, crucibles, and ring stands. Other tools described include balances, pipettes, clamps, and spatulas for accurately manipulating and transferring chemicals. Safety precautions are noted for several pieces of equipment.
Laboratory apparatuses and equipment are indispensable tools in the laboratory. Their uses enable students to conduct accurately and systematically the experiments assigned to them for the day.
Laboratory apparatuses and equipment are indispensable tools in the laboratory. Their uses enable students to conduct accurately and systematically the experiments assigned to them for the day.
Laboratory apparatus and safety measuresjulielazarte
This Slides Share discuss common laboratory apparatus as well as safety measures while inside a laboratory room. It includes images so that students would be able to visualize the equipment.
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
A Bunsen burner is a device used in the laboratory in producing a hot clean flame by burning a fuel gas in air.
Glass bends are important tools in any laboratory work.
Laboratory apparatus and safety measuresjulielazarte
This Slides Share discuss common laboratory apparatus as well as safety measures while inside a laboratory room. It includes images so that students would be able to visualize the equipment.
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
A Bunsen burner is a device used in the laboratory in producing a hot clean flame by burning a fuel gas in air.
Glass bends are important tools in any laboratory work.
This is a ppt by me Sarah on Chemistry. In this ppt you will learn what is chemistry, why we study chemistry and things in chemistry! Hope you enjoy!!!!!!
How to Write a Business Case: 4 Steps to a Perfect Business Case TemplateWorkfront
Yes, poor planning and lackluster top management support can kill otherwise well-built projects, but right up there is also the inability to make a convincing business case for your project. Luckily for you, the presentation provides a step-by-step guide for not only building a strong business case, but creating a business case template you can use again and again. Let’s get started…
Beaker
Beakers hold solids or liquids that will not release gases when reacted or are unlikely to splatter if stirred or heated.
Beaker Tongs
Beaker tongs are used to move beakers containing hot liquids.
Erlenmeyer Flask
Erlenmeyer flasks hold solids or liquids that may release gases during a reaction or that are likely to splatter if stirred or heated.
Florence Flask
Rarely used in first year Biochemistry, it is used for the mixing of chemicals. Narrow neck prevents splash exposure.
Graduated Cylinder
A graduated cylinder is used to measure volumes of liquids.
Test Tubes
Test Tube Holder
A test tube holder is useful for holding a test tube which is too hot to handle.
Test Tube Brushes
Test tube brushes are used to clean test tubes and graduated cylinders.
Forcing a large brush into a small test tube will often break the tube.
Test Tube Racks
Test tube racks are for holding and organizing test tubes on the laboratory counter.
After washing flip test tube over on wooden peg to dry.
Rubber Stoppers
Rubber stoppers are used to close containers to avoid spillage or contamination.
Glass Stir Rod
A glass rod is used to manually stir solutions. It can also be used to transfer a single drop of a solution.
Forceps
Forceps (or tweezers) are used to pick up small objects.
Funnel
A funnel is used to aid in the transfer of liquid from one vessel to another.
Watch Glass
A watch glass is used to hold a small amount of solid, such as the product of a reaction.
Wash Bottle
A wash bottle has a spout that delivers a wash solution to a specific area. Distilled water is the only liquid that should be used in a wash bottle.
Weighing Boat
Weighing boats are used to weigh solids that will be transferred to another vessel.
Bunsen Burner
Bunsen burners are used for the heating of nonvolatile liquids and solids.
Evaporating Dish
The evaporating dish is used for the heating of stable solid compounds and elements.
Crucible
Crucibles are used for heating certain solids, particularly metals, to very high temperatures.
Clay Triangle
The clay triangle is used as a support for porcelein crucibles when being heated over a Bunsen burner.
Crucible Tongs
For handling hot crucibles; also used to pick up other hot objects. NOT to be used for picking up beakers!
Ringstands and their Components
Ringstand
Ringstands are a safe and convenient way to perform reactions that require heating using a Bunsen burner.
Ringstands and their ComponentsUtility Clamps
Utility clamps are used to secure test tubes, distillation columns, and burets to the ringstand.
Ringstands and their ComponentsWire Gauze
Wire gauze sits on the iron ring to provide a place to stand a beaker.
Ringstands and their ComponentsDouble Buret Clamps
Double Buret clamps are used to burets – long graduated tubes used in titration.
Triangular File
Triangular files are used primarily to cut glass rod, a skill that your instructor will share with you when it becomes useful.
Litmus Paper
Red litmus paper is used to identify ba
I have attached here with 104 slides about Laboratory equipments and uses, Common laboratory techniques, Substances available in a laboratory (I. Solid II. Liquids III.Metals) Safety symbols and Lab safety
I have attached here with 104 pages of PDF about Laboratory equipments and uses, Common laboratory techniques, Substances available in a laboratory (I. Solid II. Liquids III.Metals) Safety symbols and Lab safety
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
1. BHS Chemistry
Lab Equipment
Adapted from presentations by Stephen L. Cotton, Charles Page High School
and Mrs. Parris, Galax High School
2. Beaker
• Beakers hold solids or liquids.
• Very poor accuracy – should only be used to
estimate volume
• Note the size capacity (250 mL in this case)
There are
four sizes
of beakers
for you to
use in your
lab desk.
3. Beaker Tongs Beaker tongs are
used to move
beakers
containing hot
liquids.
Note the rubber
coating to
improve grip on
the glass beaker
- do NOT hold
this in a burner
flame.
4. Bunsen Burner
Bunsen burners are used for heating
nonvolatile liquids and solids.
Make sure
the hose is
snug on
the gas
outlet and
that there
are no
cracks in
the hose.
5. Analytical Balance
Make sure
balance is zeroed
(reads zero)
before beginning.
You can do this
with the “zero”
function.
Never place chemicals directly onto
the balance pan. Use a weigh paper
or dish.
6. Crucible and cover
Crucibles are used
for heating certain
solids, particularly
metals, to very high
temperatures. The
cover can be used to
contain any smoke
particles.
7. Crucible Tongs
For handling hot
crucibles; also used to
pick up other hot
objects - NOT to be
used for picking up
beakers!
8. Erlenmeyer Flask
Erlenmeyer flasks
hold solids or liquids
that may release
gases during a
reaction or that are
likely to splatter if
stirred or heated.
Note the size
9. Evaporating Dish
The evaporating
dish is used for
heating stable solid
compounds and
elements, as well
as for evaporating
nonvolatile
solutions.
10. Florence Flask
Rarely used in first
year chemistry, it is
used for the mixing
of chemicals. Narrow
neck prevents splash
exposure.
12. Funnel
A funnel is used
to aid in the
transfer of
liquids from one
vessel to
another, and will
hold filter paper
while filtering. It
may be glass or
plastic.
13. Graduated Cylinder
A graduated
cylinder is used to
more accurately
measure volumes
of liquids (probably
your best everyday
measuring tool)
there are three
sizes in your desk:
10 mL, 50 mL and
100 mL.
14. Buret
A buret is used to
very accurately
measure volumes
of liquids and
dispense small
measurable
amounts of liquids.
15. Pipet
A pipet is used to
very accurately
dispense volumes
of liquids. We use
a pipet bulb or a
delivery tube to
dispense the liquid.
Never use your
mouth to draw
liquid into the pipet.
16. Glass Dropper Pipet
A droper pipet is
used to transfer a
small volume of
liquid, usually one
drop at a time.
On top of each pipet is a “rubber bulb” – never
put your mouth on the dropper to provide
suction
18. Pipe Stem (Clay) Triangle
The pipe stem
triangle is used as a
support for crucibles
when being heated
over a lab burner.
It can also be used to
support a funnel
when filtering.
19. Reaction Plates
Reaction plates (or
multi-well plates) are
used when we want to
perform many small
scale reactions at one
time.
It is like having lots of
test tubes available at
one time.
20. Ringstands and their Components
Ringstands are a
safe and convenient
way to perform
reactions that
require heating
using a lab burner.
Never put them in
the cabinet if they
are too hot to hold
as it can cause a
fire.
21. Ringstands and their Components
Metal Ring
Metal rings connect to
a ringstand, and
provide a stable,
elevated platform for a
beaker to be heated.
Will also hold a pipe
stem triangle and
funnel during filtering
22. Ringstands and their Components
Test tube/Utility Clamps
Test tube clamps are
used to secure test
tubes, distillation
columns, and burets
to the ringstand.
Image source xump.com
23. Ringstands and their Components
Wire Gauze
Wire gauze sits on the
iron ring to provide a
place to stand a
beaker.
On older wire gauze,
the white material was
asbestos – currently it
is a ceramic.
24. Scoopula/Spatula
Spatulas are used to transfer solid chemicals.
Never insert a spatula directly into a reagent
bottle.
Chemicals should never be transferred with
your bare hands.
25. Stirring Rod
The stirring rod is
used to:
a) manually stir
solutions;
b) assist in pouring
liquids; and
c) to transfer a
single drop of a
solution.
Rubber policeman tip
is used to remove
precipitates.
Stir with this end.
26. Flint Lighter / Striker
These strikers are
used to light lab
burners.
The flints on
strikers are
expensive. Do not
operate the striker
repeatedly just to
see the sparks!
27. Test Tubes – we commonly use 2 sizes:
18 x 150 mm
13 x 100 mm
Ignition
Tube
(25 x 200 mm
– I’ll use these
in demos.)
Test tubes are used to mix chemicals, and also
to heat chemicals. Only heat test tubes that are
Pyrex or Kimax.
28. Rubber Stoppers
Rubber stoppers are
used to seal a
reaction vessel,
which could be a
flask or a test tube.
Stoppers can also be
used to force gaseous
products of a reaction
to flow into a specific
location.
29. Test Tube Brushes
Test tube brushes are
used to clean test
tubes and graduated
cylinders.
Forcing a large brush
into a small test tube
will often break the
tube. Don’t worry
about drying the
inside of a test tube.
Small test tube brush
Large test tube brush
30. Test Tube Holder
A test tube holder
is useful for
holding a test
tube which is too
hot to handle.
Knowing where
to hold this piece
of equipment (at
the end) is
important!
31. Test Tube Racks
Test tube racks are for holding and organizing
test tubes on the laboratory counter.
32. Wash Bottle
Wash bottles are used to rinse solids from other
equipment when trying to conserve (keep all of)
the solid—for instance, from the inside of a test
tube.
33. Test Tube Racks
Test tube racks are for holding and organizing
test tubes on the laboratory counter.
35. Mortar & Pestle
A mortar & Pestle is
used to grind solids
into fine powders to
speed the mixing or
reaction process.
The mortar & pestle
is a thicker
porcelain that is
more heavy duty
than the
evaporating dish.
36. Watch Glass
A watch glass is
used to hold a
small amount of
solid, such as the
product of a
reaction.
Can also be used
as a cover for an
evaporating dish or
beaker.
37. Weigh Boat
Weigh boats are
used to weigh solids
that will be
transferred to
another vessel.
Never put chemicals
directly on the
balance – it leaves
residue and can
harm the balance
and contaminate the
chemicals.