1. The type of cleavage strategy used by an embryo can have an impact in how cells within that embryo specify its cells. Give one example. Use drawings to illustrate your points. 2. All the cells within an embryo have the exact same genome. Describe two strategies that an embryo uses to establish different cell fates. 3. Describe a typical signaling cascade. Include the key components. You can draw if it helps you illustrate this point. 4. What does this diagram illustrate? Give an example of when this is important in a developmental context. A single cell produces a multicellular organism through multiple stages of embryonic and postnatal development Patterns of cleavage can impact how cells within the embryo specify its cells (F) Gastrulation gives rise to the three germ layers through cell fate determination Different cell types are made through differentially expressed genes which can be regulated by various mechanisms Pioneer transcription factors are thought to significantly alter chromatin accessibility and impact cell fate determination Cell fate can be regulated by communicating with other cells or the environment through direct or indirect mechanisms Paracrine factors can activate cascades of intracellular signaling to induce changes in gene expression Sry and Sox9 (testis) and -catenin (ovary) pathway is necessary and sufficient for mammalian sex determination.