033 uptakes of spio by murine
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The document describes an experiment examining the uptake of different types of superparamagnetic iron oxide particles (SPIO) coated with various materials by murine peritoneal macrophages and monocytes. Mice were injected with different SPIO coated with chondroitin sulfate, dextran, amino-silane, lipids, or lipid encapsulated and amino-silane coated. After 24 hours, peritoneal macrophages and monocytes were isolated from the mice and examined under light microscopy to analyze uptake of the various SPIO formulations.
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110 electron beam tomography
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Acc presentation lipid coated(2)
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068 from vulnerable plaque to vulnerable patient
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1) The document discusses evidence that atherosclerosis may be an autoimmune disease triggered by heat shock proteins (HSPs) like HSP60 and HSP70.
2) Studies in mice show these HSPs are expressed early at lesion-prone sites and by endothelial cells, then decrease in older mice with advanced lesions.
3) Macrophages and T-cells in lesions also express these HSPs, suggesting an immune response role in atherosclerosis development and progression.
250 patients with very abnormal test results
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2) Histologic studies show that coronary artery calcium occurs in all types of plaques, including stable (43%), rupture-prone (54%), and erosion-prone (77%) plaques.
3) Therefore, coronary artery calcium detected by imaging is a marker of atherosclerotic plaque burden but not necessarily a marker of stable or unstable plaque, as there is considerable overlap in calcium presence between different plaque types.
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046 respiratory infection of apoe ko mice and plaque inflammation
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165 pressure wire
This document reports on a study that used a pressure wire to measure intracoronary temperatures in patients referred for cardiac catheterization. Temperature mappings were recorded in various coronary arteries. Measurements were taken in patients presenting with conditions such as unstable angina, stable angina, prior myocardial infarction, and no cardiac symptoms. The data collected included temperature, location of any stenotic lesions, and patient demographics and risk factors. Statistical analyses were also performed on the temperature readings.
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This document discusses approaches to identifying and managing coronary risk. It states that the primary goal should be preventing acute cardiac events through intensive risk factor reduction for all patients with clinically apparent heart disease. Additionally, it notes that one third of sudden cardiac deaths and heart attacks occur in previously asymptomatic individuals with undiagnosed risk factors or pre-clinical disease. The document proposes identifying high-risk asymptomatic individuals through testing to provide prevention. It presents a risk stratification approach using testing like CRP, cholesterol, glucose and imaging to guide different levels of risk factor reduction and management.
Acc presentation (1)
This document describes a study that introduces a non-invasive method for imaging macrophage infiltration in inflamed atherosclerotic plaques using superparamagnetic iron oxide (SPIO) nanoparticles and MRI. The researchers injected SPIO into hypercholesterolemic and normal rabbits and found that SPIO profoundly accumulated in areas of macrophage infiltration in the atherosclerotic plaques, as confirmed by histology. SPIO-enhanced MRI was able to identify these inflamed plaques non-invasively. The results suggest SPIO-enhanced MRI can be a novel method for detecting rupture-prone inflamed plaques associated with heart attacks and strokes.
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Similar to 033 uptakes of spio by murine
Acc presentation lipid coated(2)
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Acc presentation lipid coated(2)
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This study examined the uptake of a superparamagnetic iron oxide (SPIO) particle called CNS1-1D by mouse peritoneal macrophages. Mice were pre-treated to stimulate macrophage proliferation. CNS1-1D was injected into the peritoneum and macrophages were harvested 24 hours later. The macrophages were cultured and observed under light and UV light microscopy over 24-48 hours. Some macrophages that had taken up SPIO could be separated magnetically. A fluorometry study also showed decreasing SPIO fluorescence in macrophages over a concentration gradient and over 3-6 hours of incubation, possibly due to wash out or cell wall damage.
112 macrophage uptake of cns1 1 d
This study examined the uptake of a superparamagnetic iron oxide (SPIO) particle called CNS1-1D by mouse peritoneal macrophages. Mice were pre-treated to stimulate macrophage proliferation. CNS1-1D was injected into the peritoneum and macrophages were harvested 24 hours later. The macrophages were cultured and observed under light and UV light microscopy over 24-48 hours. Some macrophages showed fluorescence from the labeled SPIO inside the cells and could be separated magnetically. A fluorometry study also showed decreasing SPIO fluorescence in macrophages over a concentration gradient and after 3 and 6 hours of incubation.
112 macrophage uptake of cns1 1 d
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scp-presentations-210514064537.pptx
Single cell proteins (SCP) are dried microorganism cells that can be used as a protein supplement for humans and animals. SCP is produced through fermentation of fungi, yeast, algae or bacteria in large vessels, followed by harvesting, post-harvest treatment, and processing to isolate the protein. SCP shows potential as a sustainable protein source due to microbes' ability to utilize various substrates and generate protein quickly without relying on arable land.
SINGLE CELL PROTEIN
This document discusses single cell protein (SCP), which refers to protein extracted from microorganisms like yeast, algae, fungi and bacteria that can be used as a protein supplement for humans and animals. It provides details on the history of SCP, production methods using various microorganisms, advantages like rapid growth and high protein content, disadvantages like possible toxins and poor digestibility, and applications as a food supplement, in health products, cosmetics, and animal feed. Spirulina is highlighted as a commonly used algae for SCP production due to its high protein content and ease of harvesting. The document concludes that SCP production is still developing but has potential as a sustainable food source.
118 cell culture study
Cell culture studies showed that macrophages engulfed superparamagnetic iron oxide (SPIO) particles, which initially elevated free radicals but levels returned to baseline. Lipid-coated SPIO induced less nitric oxide production in macrophages than dextran-coated SPIO or Feridex. Preliminary results indicate a significant difference in nitric oxide levels produced by macrophages after ingesting different forms of SPIO.
Cell culture study
Cell culture studies showed that macrophages experience a transient increase in free radicals when ingesting dextran-coated SPIO particles, but levels return to baseline. Testing showed that lipid-coated SPIO induced less nitric oxide production in macrophages than dextran-coated SPIO or Feridex. Preliminary results indicate a significant difference in nitric oxide levels produced by macrophages depending on whether they ingest dextran-coated or lipid-coated SPIO particles.
118 cell culture study
Cell culture studies showed that macrophages experience a transient increase in free radicals when ingesting dextran-coated SPIO particles, but levels return to baseline. Testing showed that lipid-coated SPIO induced less nitric oxide production in macrophages than dextran-coated SPIO or Feridex. Preliminary results indicate a significant difference in nitric oxide levels produced by macrophages depending on whether they ingest dextran-coated or lipid-coated SPIO particles.
Cell cloning, animal cell culture
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
Immunodulators Screening methods
Immunomodulator screening method
In-vivo and in-vitro methods
Arthus hypersensitivity reaction, mitogen-induced lymphocyte proliferation
Quinolones
nalidixic acid, the quinolones, the naphthyridines & the cinnolines, Classification, ISOSTERIC REPLACEMENT
DNA gyrase (Topo II) SAR- substitution variation, Ciprofloxacin
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Single Cell Protein - Slideshare PPT
Single cell proteins (SCP) are dried cells of microorganisms that can be used as protein supplements for humans and animals. SCP production was first commercialized in the 1950s using bacteria cultured on methanol. Common microorganisms used for SCP production include fungi, yeast, algae and bacteria. Production involves selecting a suitable microorganism strain, fermenting it under controlled conditions, harvesting the cells, processing them, and isolating the protein. SCP have potential applications as nutritional supplements, health foods, and animal feed due to their protein and nutrient content.
NATURAL POLYMER - ORIGIN AND APPLICATIONS
Natural polymers are biodegradable, biocompatible substances found in nature that can be used for pharmaceutical applications. They include polysaccharides from plants like cellulose, starch, agar; animals like chitin, proteins like collagen, gelatin; and microbes like xanthan gum. Natural polymers are advantageous because they are low cost, environmentally friendly and locally available. They can be used to develop drug delivery systems like nanoparticles, microparticles, hydrogels for controlled release. Common natural polymers and their pharmaceutical uses are discussed.
Natural polymer :origin and applications
This document provides an overview of natural polymers, including their sources, classification, mechanisms of polymerization, modeling, advantages, disadvantages, applications, and marketed products. Natural polymers include polysaccharides from plants (cellulose, starch, agar), animals (chitin, xanthan gum), and microbes (alginate, pectin). They are biodegradable, biocompatible, non-toxic, and locally available. Natural polymers find applications in drug delivery, packaging, and as excipients. Examples of uses include starch nanoparticles for controlled drug release and chitosan nanoparticles as carriers for anticancer drugs.
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptx
DEVELOPEMENT AND USES OF TRANSGENIC ANIMALS:
■Definitions about Transgenic Animals (or) Genetically Modified Animals(GMO).
■History and Developements of Transgenic Animals(Yearwise:1907-2017)
■Different Methods used for developement of Transgenic animals:
1.Microinjection Method
2.Retro Viral Method
3.Embryonic Stem cell method
■Applications of Transgenic Animals
■Advantages of Transgenic Animals
■Disadvantages of Transgenic Animals
■References.
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Hypoglycemic activity of_fermented_mushroom_of_coprinus_comatus_rich_in_vanadium
This document discusses a study that investigated the hypoglycemic effects of fermented mushroom of Coprinus comatus rich in vanadium (CCRV) in mice. The study found that CCRV decreased blood glucose and HbA1c levels in alloxan-induced hyperglycemic mice and improved glucose tolerance in normal mice. CCRV also inhibited adrenaline-induced increases in blood glucose. The body weights of hyperglycemic mice increased after treatment with CCRV. In contrast, fermented mushroom of C. comatus without vanadium and sodium vanadate alone did not show significant hypoglycemic effects. The results suggest that lower doses of vanadium combined with C. comatus
Algae and bacteria scp
This document discusses bacteria and algae as sources of single cell protein (SCP). It provides background on SCP, describing how certain microorganisms like yeast, fungi, algae and bacteria grown on carbon sources can be used to produce protein for human and animal consumption. The document then discusses the history of SCP production and some key microorganisms used, focusing on production using algae like Spirulina and bacteria like Methylophilus methylotrophus. It outlines the overall production processes for both algal and bacterial SCP.
Similar to 033 uptakes of spio by murine (20)
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Hypoglycemic activity of_fermented_mushroom_of_coprinus_comatus_rich_in_vanadium
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Zahi A. Fayad is an Associate Professor who studies molecular imaging of atherosclerosis using MRI. His research focuses on developing targeted contrast agents to noninvasively detect unstable plaque. Some agents under investigation include annexin A5 labeled with a radioisotope to detect apoptosis, FDG-PET to assess plaque activity, and fibrin-targeted and MMP-targeting Gd-based contrasts. Additional work involves lipid-based particulate agents using reconstituted HDL or iron oxide nanoparticles. The goal is to improve MRI detection sensitivity and specificity for high-risk plaque characterization.
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#1 killer of human beings in the 21st century
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1031 talk2
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033 uptakes of spio by murine
- 1. Uptakes of SPIO by Murine Macrophages and Monocytes Daniel Chan University of Colorado Health Sciences Center 12-19-2001
- 2. Synthesis of SPIO • 1. SPIO coated with chondroitin sulfate • 2 SPIO coated with standard dextran (6K) • 3 SPIO coated with Amino-silane • 4 SPIO coated with lipids • 5 Lipid encapsulated SPIO coated with amino-silane
- 3. Uptakes of SPIOs by murine peritoneal macrophages and monocytes • Normal mice were treated with mineral oil by ip injection. • 24 hours later, equal amount of various SPIOs were injected intraperitoneally. • 24 hours later, peritoneal macrophages and monocytes were isolated. • Uptakes of various SPIOs were examined by light microscopy.
- 4. Uptake by Mouse Macrophages SPIO coated with chondroitin sulfate
- 8. Uptake by Mouse Macrophages SPIO coated with standard Dextran (6K)
- 12. Uptake by Mouse Macrophages SPIO coated with amino-silane
- 16. Uptake by Mouse Macrophages SPIO coated with lipids
- 20. Uptake by Mouse Macrophages Lipid encapsulated SPIO coated with amino-silane
- 24. Uptake by Mouse macrophages SPIO coated with CS
- 27. Uptake by Mouse Macrophages SPIO coated with Dextran (6k)
- 30. Uptake by Mouse Macrophages SPIO coated with Amino-silane
- 33. Uptake by Mouse Macrophages SPIO coated with lipids
- 36. Uptake by Mouse Macrophages Lipid encapsulated SPIO coated with amino-silane