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Biosafety Level 4

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Biosafety Level 4

  1. 1. BIOSAFETY LEVEL 4<br />Presented by :<br />SheetalNarkar<br />Pooja Lad<br />
  2. 2. BIOSAFETY<br />The application of the combination of laboratory practice and procedures, laboratory facilities and safety equipment when working with potentially infectious micro-organisms<br />
  3. 3. BIOSAFETY LEVEL<br />A biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facility<br />There are mainly 4 biosafety levels <br />
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  6. 6. BIOSAFETY LEVEL 4<br />This level is required for work with dangerous and exotic agents that pose a high individual risk of :<br /><ul><li>aerosol-transmitted laboratory infections,
  7. 7. agents which cause severe to fatal disease in humans for which vaccines or other treatments are not available</li></ul>Eg. Lassa fever, Ebola hemorrhagic fever, hemorrhagic diseases<br />
  8. 8. CODE OF PRACTICES<br />The two-person rule should apply, whereby no individual ever works alone. This is particularly important if working in a Biosafety Level 4 suit facility<br />Laboratory protective clothing, head coverings, scrub suits, coveralls, shoe cover etc<br />Respiratory protective equipment<br />
  9. 9. CODE OF PRACTICES<br /> A complete change of clothing and shoes is required prior to entering and upon exiting the laboratory<br />Personnel must be trained in emergency situations like in the event of personnel injury or illness.<br />
  10. 10. LABORATORY DESIGN AND FACILITY <br />Primary Containment<br /><ul><li>Class III cabinet laboratory
  11. 11. Suit laboratory</li></li></ul><li>Emergency Power<br />UV light and vacuum room<br />
  12. 12. Controlled Access<br />Controlled Air System<br />
  13. 13. Decontamination of effluents<br />Sterilization of waste and materials<br />
  14. 14. THANK YOU<br />

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