This presentation clearly explains you about the Biosafety Level 2. Its includes the requirements, practices, organisms, equipment and uses of Biosafety level 2.

1. BIOSAFETY
LEVEL 2
BY
S.GANGASHRI,
BIOTECHNOLOGY

2. CONTENTS
Definition
Classification
Biosafety level 2
Requirements
Standard Microbiological
Practices
Safety Practices
Safety Equipments
Organisms
Uses

3. DEFINITION
• A biosafety level (BSL), or pathogen/protection level,
is a set of biocontainment precautions required to
isolate dangerous biological agents in an enclosed
laboratory facility. The levels of containment range
from the lowest biosafety level 1 (BSL-1) to
the highest at level 4 (BSL-4).
• Biosafety levels (bsl) are used to identify the
protective measures needed in a laboratory setting
to protect workers, the environment, and the public.
Activities and projects conducted in biological
laboratories are categorized by biosafety level.

4. CLASSIFICATION
BIOSAFET
Y
LEVEL 1
BIOSAFET
Y
LEVEL 2
BIOSAFET
Y
LEVEL 3
BIOSAFET
Y
LEVEL 4

5. BIOSAFETY LEVEL 2
• Biosafety level-2 laboratories are the laboratories that are used
for the tasks involving microbial agents of moderate potential
hazards to the laboratory personnel, the environment, and the
agent.
• However, the infectious agents or the toxins might pose a
moderate danger if accidentally inhaled, swallowed, or exposed
to the skin.
• The precautions associated with biosafety level-2 are
comparatively more extensive than bsl-1, but bsl-1 and bsl-2
laboratories are generally considered as basic laboratories.

6. REQUIREMENTS
• BSL-2 laboratories like BSL-1 laboratories are not necessarily separated
from the general traffic patterns in the building.
• However, access into the laboratory is limited while bsl-2 experiments are
in progress.
• The annual inspection of the laboratories is also an important part of the
bsl-2 requirements. These might include changing the filters or
replacement of some devices.
• The work is mostly conducted on sterilized bench tops except for some
processes that might form aerosols. The latter is conducted in safety
cabinets.
• The precautions to be followed in bsl-2 include all the precautions of the
bsl-1 and some additional precautions.

7. STANDARD MICROBIOLOGICAL PRACTICES
• All the laboratory personnel must wash their hands after using viable microorganisms and before
leaving the laboratory.
• Eating, drinking, smoking, and handling contact lenses in the laboratory are strictly prohibited.
• Mechanical pipetting should be done instead of mouth pipetting.
• All contaminated cultures, glassware, plastic ware, and biologically contaminated waste must be
treated as bio-hazards and thus, autoclaved.
• Work surfaces must be decontaminated with disinfectant at the end of the day or after any spills or
splashes.
• Used hypodermic syringes and needles, pasteur pipettes, razor blades, contaminated broken glass,
and blood vials are treated as medical waste and discarded in puncture-resistant sharps disposal
containers.

8. SAFETY PRACTICES
• People with increased risk of acquiring infections like the immune-
compromised and pregnant individuals should not be allowed to
enter the BSL-2 laboratories while the laboratories are at work.
• An annual review of the bsl-2 manual should be done to update
the guidelines.
• Documented policies and procedures should be established that
limit the entrance to individuals who know of the potential hazards
and are appropriately trained.
• A biohazard symbol is placed on pieces of equipment where
biohazardous materials are used or stored.

9. SAFETY EQUIPMENTS
• Protective coats are to be worn while entering the laboratory and then removed and kept in
the laboratory post work.
• The laboratory design should be made such that it can be easily cleaned and
decontaminated with minimum nooks and corners.
• The laboratory doors should be closed whenever work with hazardous biomaterials is
conducted.
• An autoclave must be available.

10. ORGANISMS
The organisms that require BSL-2 laboratories include the pathogenic
strains of
• E. Coli,
• Staphylococcus,
• Salmonella,
• Plasmodium falciparum,
• Toxoplasma, and
• Herpes simples viruses.
The allocation of organisms to the laboratories, however, might differ
depending on the risk assessment.

11. USES
• Biosafety level-2 laboratories are mostly used for routine analysis and
culture of moderately hazardous agents.
• Besides, some of the laboratories used for teaching and training
purposes are also bsl-2 laboratories.

12. THANK
YOU!