Hybridization is the process of interbreeding between individuals of different species or genetically divergent individuals of the same species to form a hybrid. The key steps in the hybridization process include emasculation to remove anthers from bisexual flowers, tagging the female parents with details of the cross, and bagging flowers to prevent self-pollination. There are several types of hybrids including intervarietal, single cross, double cross, and three way cross hybrids formed by crosses between inbred lines and F1 hybrids. Distant hybridization can involve intergeneric or interspecific crosses between parents of different genera or species.

1. Submitted by: Pratyush Panda[201]
Guided by: Dr. Swapnil
HYBRIDIZATION
TECHNIQUES

2. INTRODUCTION TO HYBRIDIZATION:
Hybridization is the process of interbreeding between
indivisuals of different species (interspecific hybridization)
or individuals from the same species genetically
divergent(intraspecific hybridization).
“Hybrid”- Hybrid is formed by combining the qualities of
two different breeds, species or genera through sexual reproduction.

3. EMASCULATION: The process of removing anthers from bisexual flowers
without affecting the female reproductive part (pistil).
PROCEDURE OF HYBRIDIZATION

4. TAGGING:The female parents are then labelled properly. The labellings should bear
the following information.Serial number Details of male parents and female
parents.Date of emasculation and crossing.
BAGGING: A process used in plant breeding to prevent self
pollination in bisexual flowers.

5. Types Of Hybrid
1.Intervarietal Hybridisation —In this type of hybridization,
two parents from the same species (may be from two •arietie€,
strains or races of the same species) are crossed.

6. Single Cross Hybrids-A hybrid plant results from a cross of two
genetically different plant.
Double Cross Hvbrids-Results from the cross between two
different F1 hybrids.
Three Way Cross Hybrid-Hybrid formed by a cross between
single cross hybrid and an inbred.
Distant Hybridisation includes:
❁ Intergeneric hybridization - crossing between
parents from the two different genera.
❁ Interspecific hybridization - crossing between
parents from two different species.

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