BIOLOGIA MOLECULAR

1. Maria Camila Rodriguez Morales

4. INTRODUCTION
Schematic diagram
showing generally the
various molecular
biology techniques used
for the characterization
of microorganisms from
environmental samples
We need that
techniques to
diagnose
disease
genetic
mutations.

6. Testosterone is a key
hormone in the growth
and development of
human beings as it has
different functions.

7. The comparison between
different tandem MS methods
and a sensitive testosterone
RIA illustrates that there are
immunoassays that deliver
clinically useful information in
prepubertal and pubertal
children.
Dr. Yalow was a medical physicist who co-discovered the
radioimmunoassay, an exquisitely sensitive means of using
“radioactive tracers” to measure hormones in the
bloodstream, such as insulin, thyroid, reproductive and
growth hormones, as well as levels of vitamins, viruses and
many other substances in the body.

8. Compared to the pediatric
reference values determined by LC-
MS/MS, the sensitive testosterone
RIA delivered similar testosterone
values across the different pubertal
stages.

9. I think that new is very important
and interesting because this
method of study reveals damage
and alterations in hormones each
person can make a more personal
study focused on the needs of each
person.
STUDENT OBSERVATION

11. Muscle changes include a reduction
of fast muscle fibers type 2
mitochondrial DNA damage, loss of
motor units in the spinal cord,
reducing testosterone and estrogen
accelerates the loss of muscle mass
and growth hormone also affects
loss of muscle mass in the elderly.

12. Sarcopenia is not completely
reversed with exercise but physical
inactivity accelerates the loss of
muscle mass.

13. The defect found in the
study helps to improve our
understanding of the
mechanism of sarcopenia,
researchers would like
them to study more
thoroughly this mechanism
to find a more efficient
treatment.

14. STUDENT OBSERVATION
I think the study is
important because it is the
beginning of a lot of new
studies to try to
understand the diseases
related to old age and to
have in the future a better
quality of life.

16. Both studies results
obtained through ria which
helps to better study a
specific gene can
determine a disease and to
decipher variables changes
such as mutations in the
genes.

17. Radioimmunoassay
techniques solid phase
(RIEFS) have been valuable
for quantify antibodies
against bacteria and virus.
In the indirect technique
Solid phase
radioimmunoassay
generally purified antigen
is added
directly to this stage.

18. With RIA technique it is
effective to determine
which treatment options
may have on each disease.

19. • http://www.healthcanal.com/male-reproductive/low-
testosterone/64413-sensitive-ria-measures-testosterone-
concentrations-in-prepubertal-and-pubertal-children-comparable-to-
tandem-mass-spectrometry.html
• http://www.sciencedaily.com/releases/2015/06/150608083107.htm