SlideShare a Scribd company logo
1 of 46
Download to read offline
Research, The Research
   Process, Research
      Methodology
      LECTURE 2 (BIOLOGY 199)
      MARILEN M. PARUNGAO
RESEARCH



viewed as a problem-solving activity
 follows a logical series of steps
(research process) which makes it
different from other problem-solving
activities
Research Methodology
             (Leedy, 1997)

the core concept underlying the research process

the methodology controls the study itself and the processes needed to
realize the study

the methodology controls and dictates the acquisition of data,
arranges them in logical relationships, sets up a means of refining the
raw data, contrives an approach so that meanings that lie below the
surface of those data become manifest, and finally issues a
conclusion or series of conclusions that lead to an expansion of
knowledge

the entire process is a unified effort as well as an appreciation of its
component parts
The Process of Research:
         Logical Steps
THE RESEARCH PROBLEM

  PROBLEM IDENTIFICATION (LOOKING FOR A TOPIC)

  CHARACTERISTICS OF A GOOD RESEARCH PROBLEM

  RESEARCHABILITY OF THE PROBLEM




FORMULATION OF RESEARCH OBJECTIVES

  DEFINITION OF RESEARCH OBJECTIVES

  CHARACTERISTICS OF RESEARCH OBJECTIVES
THE RESEARCH PROBLEM

THE HEART OF THE RESEARCH
PROJECT

REQUIREMENT: TO STATE THE
PROBLEM WITH UNWAVERING
CLARITY, PRECISION

WHAT IF I SIMPLY CANNOT FIND A
GOOD PROBLEM?
IDENTIFICATION OF THE
      PROBLEM
WHERE TO FIND INTERESTING PROBLEMS...

   JOURNALS, BOOKS, ABSTRACTS (LIBRARY/TRUSTED LINKS)

   RECOMMENDATION SECTIONS OF THESES AND DISSERTATIONS/JOURNAL
   ARTICLES

   IDEAS FROM YOUR MENTOR OR PROFESSOR

   IDEAS FROM SEMINARS, RESEARCH COLLOQUIA AND CONFERENCES

   PERSONAL/FAMILY EXPERIENCES

   RARE/INTERESTING OCCURRENCES WHICH NEEDS TO BE EXPLAINED

   TOP TEN CAUSES OF MORTALITY/MORBIDITY IN YOUR LOCALITY
CHARACTERISTIC OF A
   RESEARCH PROBLEM
SHOULD BE OF GREAT INTEREST TO YOU

USEFUL FOR THE CONCERNED PEOPLE IN A PARTICULAR FIELD

POSSESS NOVELTY

LAYS FOUNDATION FOR FURTHER RESEARCH IN THE FIELD

CAN BE COMPLETED IN THE ALLOTTED TIME DESIRED

MUST USE APPROPRIATE AND UP-TO-DATE TECHNOOLOGY

DOES NOT CARRY ETHICAL OR MORAL IMPEDIMENTS
A GOOD RESEARCH
PROBLEM SHOULD BE
      SMART
 SPECIFIC, MEASURABLE, ACHIEVABLE,
       REALISTIC, TIME-BOUND
IS MY PROBLEM WORTHY
     OF RESEARCH?

EXTERNAL FACTORS
 NOVELTY AND AVOIDANCE OF
 UNNECESSARY REPETITION
 PRACTICAL VALUE OF THE
 PROBLEM
IS MY PROBLEM WORTHY
     OF RESEARCH?
PERSONAL FACTORS

  TRAINING AND PERSONAL QUALIFICATIONS

  TIME REQUIREMENTS

  AVAILABILITY OF SUBJECTS AND EQUIPMENTS

  SPECIALIZED WORKING CONDITIONS

  HAZARDS TO BE ENCOUNTERED

  RESEARCH FUNDS (COST)
STATING YOUR RESEARCH
       PROBLEM


THE RESEARCH PROBLEM MUST
BE STATED IN A CLEAR AND
COMPLETE GRAMMATICAL
SENTENCE IN AS FEW WORDS AS
POSSIBLE!
WHAT’S WRONG WITH
  THESE RESEARCH


BUSING OF SCHOOL CHILDREN

RETIREMENT PLANS OF ADULTS

EFFECT OF PHARMACEUTICALS ON EMBRYO

E. COLI AND WATER QUALITY
FORMULATION OF
  RESEARCH OBJECTIVES

RESEARCH OBJECTIVES

  REFLECT THE QUESTIONS WHOSE ANSWERS THE
  INVESTIGATOR WANTS TO STUDY YIELD TO

  CAN BE EXPRESSED EITHER IN THE FORM OF A STATEMENT OR
  A QUESTION

  SERVES AS THE STEERING WHEEL IN THE CONDUCT OF A
  RESEARCH PROJECT

  SERVES A S AGUIDE IN SPECIFYING VARIABLES TILL
  INTERPRETATION OF RESULTS
SAMPLE OBJECTIVES

TO DEVELOP AN OPTIMIZED PROTOCOL TO DETECT
FLAVIVIRUSES IN SERUM SAMPLES USING PCR



TO DETERMINE THE EFFICACY OF ORAL ADMINISTRATION
OF PROBIOTICS IN MANAGING OBESITY



TO ESTABLISH THE RELATIONSHIP BETWEEN PLANT
HEIGHT AND FERTILIZER CONCENTRATION IN CORN
IMPORTANT
CHARACTERISTICS OF
RESEARCH OBJECTIVES
PHRASED CLEARLY, UNAMBIGUOUSLY AND SPECIFICALLY


 STATED IN MEASURABLE TERMS AND DO NOT INVOLVE
                VALUE JUDGEMENT
GENERAL VERSUS SPECIFIC

GENERAL OBJECTIVE

 a generic statement which describes in broad terms
 what the study wishes to accomplish

SPECIFIC OBJECTIVE

 contain indicators on how to accomplish the stated
 objectives and therefore, gives direction to the research
 process; identifies in detail and measureable terms
 the aims of the research study
EXAMPLE

General Objectives:

   To investigate the histological effects of neem seed kernel extract on
   mouse testis

Specific Objectives

To determine/identify the changes in testes histology due to neem seed
kernel extract (NSKE) exposure

To determine the relationship between neem seed kernel extract (NSKE)
and occurrence of abnormal sperm morphology

To provide a feasible physiological basis for the anti-libido property of neem
extract
REVIEW OF RELATED
        LITERATURE
After the research problem has been identified and the objectives
formulated, a review of related literature needs to be done.

Two Important Uses:

   •
 To get acquainted with the existing studies related to the
      research to be conducted relative to:

       -   who have done the work on the problem area
       -   what has been found
       -   research design utilized
       -   statistical analysis applied
       -   problem met and how were they resolved

   •
 To establish a rationale or a theoretical or conceptual
      framework based on previous research studies done.
SCOPE AND LIMITATIONS

Researcher must be shrewd in narrowing the
scope of his study without becoming concerned
with a trivial problem
Assumptions, restrictions and limitation must be
explicit with respect to the coverage of the
study
Helps focus attention on valid objectives, &
helps minimize the dangers of over
generalization
FACTORS TO CONSIDER IN
DELIMITING THE PROBLEM
the scope of the problem

time allotted for the conduct of the study

cost and funding

cooperation/coordination needed from other institutions or
researchers

availability of research subjects

availability of equipment needed

ethical considerations
EXAMPLE

Impact of continuing education for health workers

The effect of continuing education activities conducted by the
Department of Health (DOH) for its staff on their performance

The effect of workshops/seminars conducted by the DOH for
its staff on their ability to manage the different programs of the
DOH in the field

To determine the effect of the Master Trainor’s Course
conducted by the DOH on the capabilities of the participants to
plan, implement, monitor, and evaluate the training programs
they conduct in the field
FORMULATION OF A
    TESTABLE
   HYPOTHESIS
DEFINITION

A tentative explanation for certain phenomena, or
events which have occurred or will occur (Gay,
1976)

States the researcher’s expectations concerning
the relationship between two or more variables in
the research problem

Testable statement of a potential relationship
between two or more variables (McGuigan, 1978)
CHARACTERISTICS OF A
   GOOD HYPOTHESIS

Stated in declarative form

Stated in definite terms, the relationship between
variables

Should reflect the theory or literature that it is
based on

Should be brief and to the point

Should be testable
TWO TYPES OF
       HYPOTHESIS
“RESEARCH HYPOTHESIS AND THE NULL HYPOTHESIS”
THE NULL HYPOTHESIS

Ho

Never true or established but can be possibly
disproved in the course of the experimentation

No difference relationship between the variables we
want to study

May act as a starting point and as a benchmark
against which the researcher will measure the actual
outcome of the study once the researcher has
collected the data
RESEARCH HYPOTHESIS


HA
Alternative hypothesis
Relationship is always
positive
EXAMPLES

Ho : Vitamin C does not inhibit chromosomal lagging

HA : Vitamin C does inhibit chromosomal lagging by 50%
compared to placebo



Ho : Cerebral artery bypass is as effective as standard
medical therapy

HA : Cerebral artery bypass is more effective than standard
medical therapy
TWO TYPES OF RESEARCH
     HYPOTHESIS

Non-directional – reflects a difference
between groups, but the direction of the
difference (unequal) is NOT specified


Directional – reflects a difference
between groups and the difference is
specified
IDENTIFICATION OF
  RESEARCH VARIABLES

Variable – any trait/characteristic that
manifest differences irrespective of whether
the differences are qualitative or quantitative


Qualitative – eye color, shape of teeth, sex
Quantitative – weight, height, length, light
intensity, temperature
TYPES OF VARIABLE
Independent – the treatment variable

   variables in the course of an experiment in an effort to understand the effects of
   this manipulation on some outcome (which you know as the dependent variable)

   the variable which is presumed to cause, effect, influence, or stimulate the
   outcome

Dependent – outcome variables in a research study

   refers to the outcome or response variable

Extraneous Variable – by themselves produce changes which may be mistaken to be
the effect of the independent variable being considered

   Controlled, held constant or randomized – so the effects are neutralized, cancelled
   out or equated for all conditions
TRY THIS...


PROBLEM: the effect of carbon dioxide
loading on plant morphology
Identify the:
  Independent variable
  Dependent variable
  Intervening/extraneous variable
CONSTRUCTION OF A
      RESEARCH DESIGN
represents the “plan of attack” of the researcher

   in answering the research objectives

   in obtaining all the relevant data in relation to objectives and hypothesis

the specific areas of concern in the choice of a research design are the
following

   selection and number of subjects

   control and manipulation of relevant variables

   establishment of criteria to evaluate outcomes

   instrumentation

   maximization of internal and external validity
FACTORS TO CONSIDER


research objectives
feasibility
ethical considerations
economy and efficiency
internal and external validity
INTERNAL VALIDITY

refers to extent to which investigator is able to
control the different biases affecting the study
and in the end, measures what he really intends
to measure

Did the experimental treatment really bring about
a change in the dependent variable?

Did the independent variable make a significant
difference?
EXTERNAL VALIDITY


refers to the extent to which the
investigator is able to generalize the
results of his study
Are the results applicable to groups
and environment outside of
experimental setting?
DESIGN THE TOOLS FOR
  DATA COLLECTION



Experimentation
Questionnaire
Interview schedule and forms
DESIGN THE PLAN FOR
     DATA ANALYSIS

A number of researchers think about data analysis only
after all data has been collected

Consequences:

  Some very important variables in study are either not
  measured at all or collected using a measurement
  scale which is inconsistent with desired mode of data
  analysis

  Objectives are too ambitious or non-measurable,
  given the nature of the data that were collected
THE SOLUTION...

A good practice is to construct a dummy
table


Dummy Tables – skeleton tables drawn
to help the investigator conceptualize how
the data is going to be organized and
presented after it has been collected
COLLECTION OF DATA


Essential phase of the research process
Researcher employs specialized tools,
instruments and procedures depending
upon the method designed for such
activity
DATA PROCESSING


Process the information gathered to
prepare for and facilitate analysis and
interpretation of data.
Editing of data collection forms and
coding of responses are procedures
usually done in this stage
DATA ANALYSIS AND
    INTERPRETATION

Involves quantification, description, and
classification of data
Statistics play a major role
Researcher must be familiar with basic
statistical concepts and procedures and must
know their limitations as well as the areas
where they may be appropriately applied
DRAWING CONCLUSIONS
AND RECOMMENDATIONS

Researcher summarizes the discussion
on the research findings and make a
clear concluding remarks
Researcher identifies major points that
were not raised in the present study and
could lay the framework for future
undertakings
WRITING OF RESEARCH
       REPORT

Researcher prepares report of different
activities he has undertaken together with
his findings
Report must be well-organized and
presented in proper form and style
The basic principles of technical report
writing are followed
REPORT OF RESEARCH
       FINDINGS

Publish findings in scientific journals and
news releases


Presentation of results in scientific
meetings
SO...READY TO DO
YOUR RESEARCH???

More Related Content

What's hot

What's hot (20)

Methods of Research - Research and Design (Module 1)
Methods of Research - Research and Design (Module 1)Methods of Research - Research and Design (Module 1)
Methods of Research - Research and Design (Module 1)
 
Types of Research
Types of ResearchTypes of Research
Types of Research
 
What is a Research design and its types
What is a Research design and its typesWhat is a Research design and its types
What is a Research design and its types
 
Resarch design
Resarch designResarch design
Resarch design
 
Research
ResearchResearch
Research
 
Chapter 7
Chapter 7Chapter 7
Chapter 7
 
Research Design
Research DesignResearch Design
Research Design
 
Research Design
Research Design Research Design
Research Design
 
Study designs and their advantages and limitation
Study designs and their advantages and limitationStudy designs and their advantages and limitation
Study designs and their advantages and limitation
 
Mb0034
Mb0034Mb0034
Mb0034
 
Lecture 01 - Research Methods
Lecture 01 - Research MethodsLecture 01 - Research Methods
Lecture 01 - Research Methods
 
Azam ppt research design
Azam ppt research designAzam ppt research design
Azam ppt research design
 
Types of research
Types of researchTypes of research
Types of research
 
Types of research designs
Types of research designsTypes of research designs
Types of research designs
 
Reseach design iste 2013
Reseach design   iste 2013Reseach design   iste 2013
Reseach design iste 2013
 
Educational Research Quantitative Methods (basic and applied)
 Educational Research Quantitative Methods (basic and applied)  Educational Research Quantitative Methods (basic and applied)
Educational Research Quantitative Methods (basic and applied)
 
research approach and design
research approach and designresearch approach and design
research approach and design
 
Basic research
Basic researchBasic research
Basic research
 
BASIC RESEARCH TERMINOLOGIES
BASIC RESEARCH TERMINOLOGIESBASIC RESEARCH TERMINOLOGIES
BASIC RESEARCH TERMINOLOGIES
 
Research Design
Research DesignResearch Design
Research Design
 

Viewers also liked

Quali lecture 1: Understanding the research process
Quali lecture 1: Understanding the research processQuali lecture 1: Understanding the research process
Quali lecture 1: Understanding the research process
Jari Laru
 
Overview research process
Overview research processOverview research process
Overview research process
Nursing Path
 
The Research Process
The Research ProcessThe Research Process
The Research Process
Zain Mushtaq
 
Research Process- Objective, Hypothesis (Lec2)
Research Process- Objective, Hypothesis (Lec2)Research Process- Objective, Hypothesis (Lec2)
Research Process- Objective, Hypothesis (Lec2)
Jasper Obico
 
Research Process1
Research Process1Research Process1
Research Process1
gaurav22
 
The Research Process
The Research ProcessThe Research Process
The Research Process
hisled
 
Research process best explained..
Research process best explained..Research process best explained..
Research process best explained..
Dr. Amit Joshi
 

Viewers also liked (20)

Research process - 2013 - for IS5232 module
   Research process - 2013 -  for IS5232 module   Research process - 2013 -  for IS5232 module
Research process - 2013 - for IS5232 module
 
Abdm4064 week 03 research process
Abdm4064 week 03 research processAbdm4064 week 03 research process
Abdm4064 week 03 research process
 
iResearch - The Research Process
iResearch - The Research ProcessiResearch - The Research Process
iResearch - The Research Process
 
Writing In The Health Sciences: The Research Process
Writing In The Health Sciences: The Research ProcessWriting In The Health Sciences: The Research Process
Writing In The Health Sciences: The Research Process
 
Research process notes PPT; By Muthama, Japheth Mutinda
Research process notes PPT;  By Muthama, Japheth MutindaResearch process notes PPT;  By Muthama, Japheth Mutinda
Research process notes PPT; By Muthama, Japheth Mutinda
 
Quali lecture 1: Understanding the research process
Quali lecture 1: Understanding the research processQuali lecture 1: Understanding the research process
Quali lecture 1: Understanding the research process
 
Overview research process
Overview research processOverview research process
Overview research process
 
Research process
Research processResearch process
Research process
 
How to Make your Research Process more Effective? 4 Must-Use Tools for Resear...
How to Make your Research Process more Effective? 4 Must-Use Tools for Resear...How to Make your Research Process more Effective? 4 Must-Use Tools for Resear...
How to Make your Research Process more Effective? 4 Must-Use Tools for Resear...
 
The Research Process
The Research ProcessThe Research Process
The Research Process
 
Research Process- Objective, Hypothesis (Lec2)
Research Process- Objective, Hypothesis (Lec2)Research Process- Objective, Hypothesis (Lec2)
Research Process- Objective, Hypothesis (Lec2)
 
Scaffolding the Research Process
Scaffolding the Research ProcessScaffolding the Research Process
Scaffolding the Research Process
 
Research Process and Research Design.
Research Process and Research Design.Research Process and Research Design.
Research Process and Research Design.
 
Research process
Research process Research process
Research process
 
Research process and sampling
Research process and samplingResearch process and sampling
Research process and sampling
 
Research Process1
Research Process1Research Process1
Research Process1
 
The Research Process
The Research ProcessThe Research Process
The Research Process
 
Research process best explained..
Research process best explained..Research process best explained..
Research process best explained..
 
Steps for research process
Steps for research processSteps for research process
Steps for research process
 
Research process
Research processResearch process
Research process
 

Similar to Biology 199 Lecture 2 (Research Process)

Writing A Health Research Proposal
Writing A Health Research ProposalWriting A Health Research Proposal
Writing A Health Research Proposal
Soha Rashed
 
Research problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual frameworkResearch problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual framework
Meghana Sudhir
 
Research problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual frameworkResearch problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual framework
Meghana Sudhir
 
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdfchapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
CasselDacanay
 
Bio 199 Lecture 1 (Research) 2009
Bio 199 Lecture 1 (Research) 2009Bio 199 Lecture 1 (Research) 2009
Bio 199 Lecture 1 (Research) 2009
Marilen Parungao
 
Nursing research
Nursing researchNursing research
Nursing research
Kiran
 

Similar to Biology 199 Lecture 2 (Research Process) (20)

Basic principles of research
Basic principles of researchBasic principles of research
Basic principles of research
 
Writing A Health Research Proposal
Writing A Health Research ProposalWriting A Health Research Proposal
Writing A Health Research Proposal
 
Research problem
Research problemResearch problem
Research problem
 
Writingahealthresearchproposal
WritingahealthresearchproposalWritingahealthresearchproposal
Writingahealthresearchproposal
 
RESEARCH METHODOLOGY.pptx
RESEARCH METHODOLOGY.pptxRESEARCH METHODOLOGY.pptx
RESEARCH METHODOLOGY.pptx
 
TYPES OF RESEARCHES AND ITS IMPORTANCE IN PHYSIOTHERAPY
TYPES OF RESEARCHESAND ITS IMPORTANCE IN PHYSIOTHERAPYTYPES OF RESEARCHESAND ITS IMPORTANCE IN PHYSIOTHERAPY
TYPES OF RESEARCHES AND ITS IMPORTANCE IN PHYSIOTHERAPY
 
Research problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual frameworkResearch problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual framework
 
Research problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual frameworkResearch problem, hypothesis & conceptual framework
Research problem, hypothesis & conceptual framework
 
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdfchapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
chapter1ofaresearchpaper-150708005810-lva1-app6891.pdf
 
Practical Research 1
 Practical Research 1 Practical Research 1
Practical Research 1
 
Introduction to RESEARCH Methodology
Introduction to RESEARCH MethodologyIntroduction to RESEARCH Methodology
Introduction to RESEARCH Methodology
 
APPRAISING EVIDENCE ABOUT INTERVENTIONS.pptx
APPRAISING EVIDENCE ABOUT INTERVENTIONS.pptxAPPRAISING EVIDENCE ABOUT INTERVENTIONS.pptx
APPRAISING EVIDENCE ABOUT INTERVENTIONS.pptx
 
Research problem
Research problem Research problem
Research problem
 
Parts of research paper
Parts of research paperParts of research paper
Parts of research paper
 
Research design
Research designResearch design
Research design
 
IBR 1.pptx
IBR 1.pptxIBR 1.pptx
IBR 1.pptx
 
Bio 199 Lecture 1 (Research) 2009
Bio 199 Lecture 1 (Research) 2009Bio 199 Lecture 1 (Research) 2009
Bio 199 Lecture 1 (Research) 2009
 
CRIS LUTHER's RESEARCH METHODOLOGIES COMPILATION
CRIS LUTHER's RESEARCH METHODOLOGIES COMPILATIONCRIS LUTHER's RESEARCH METHODOLOGIES COMPILATION
CRIS LUTHER's RESEARCH METHODOLOGIES COMPILATION
 
Nursing research
Nursing researchNursing research
Nursing research
 
Nursing research- rough.pdf
Nursing research- rough.pdfNursing research- rough.pdf
Nursing research- rough.pdf
 

More from Marilen Parungao

Biodiversity: Living and Non-Living Resources
Biodiversity: Living and Non-Living ResourcesBiodiversity: Living and Non-Living Resources
Biodiversity: Living and Non-Living Resources
Marilen Parungao
 
Traditional versus Modern Biotechnology (Exam 2 coverage)
Traditional versus Modern Biotechnology (Exam 2 coverage)Traditional versus Modern Biotechnology (Exam 2 coverage)
Traditional versus Modern Biotechnology (Exam 2 coverage)
Marilen Parungao
 
BIODIVERSITY: definition, levels and threats
BIODIVERSITY: definition, levels and threatsBIODIVERSITY: definition, levels and threats
BIODIVERSITY: definition, levels and threats
Marilen Parungao
 
Classical Biotechnology: FERMENTATION
Classical Biotechnology: FERMENTATIONClassical Biotechnology: FERMENTATION
Classical Biotechnology: FERMENTATION
Marilen Parungao
 
Answers to groupie_for my MBB students
Answers to groupie_for my MBB studentsAnswers to groupie_for my MBB students
Answers to groupie_for my MBB students
Marilen Parungao
 
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
Marilen Parungao
 
Lecture on nucleic acid and proteins
Lecture on nucleic acid and proteinsLecture on nucleic acid and proteins
Lecture on nucleic acid and proteins
Marilen Parungao
 
Mendelian genetics lecture quiz
Mendelian genetics lecture quizMendelian genetics lecture quiz
Mendelian genetics lecture quiz
Marilen Parungao
 
Ecology and Ecosystem Concepts
Ecology and Ecosystem ConceptsEcology and Ecosystem Concepts
Ecology and Ecosystem Concepts
Marilen Parungao
 
Chemistry of life and Metabolism
Chemistry of life and MetabolismChemistry of life and Metabolism
Chemistry of life and Metabolism
Marilen Parungao
 
NS5 Lecture 1: Environmental Ethics
NS5 Lecture 1: Environmental EthicsNS5 Lecture 1: Environmental Ethics
NS5 Lecture 1: Environmental Ethics
Marilen Parungao
 
MMB1 Lecture 1: Introduction to Biotechnology
MMB1 Lecture 1: Introduction to BiotechnologyMMB1 Lecture 1: Introduction to Biotechnology
MMB1 Lecture 1: Introduction to Biotechnology
Marilen Parungao
 
Bio 151 lectures for examination 2
Bio 151 lectures for examination 2Bio 151 lectures for examination 2
Bio 151 lectures for examination 2
Marilen Parungao
 
Microbial control lecture reference
Microbial control lecture referenceMicrobial control lecture reference
Microbial control lecture reference
Marilen Parungao
 
Biology 120 lectures for 2nd exam 2012 2012
Biology 120 lectures for 2nd exam 2012 2012Biology 120 lectures for 2nd exam 2012 2012
Biology 120 lectures for 2nd exam 2012 2012
Marilen Parungao
 
Bio 196 fbyz calendar t3 4
Bio 196 fbyz calendar t3 4Bio 196 fbyz calendar t3 4
Bio 196 fbyz calendar t3 4
Marilen Parungao
 

More from Marilen Parungao (20)

Biodiversity: Living and Non-Living Resources
Biodiversity: Living and Non-Living ResourcesBiodiversity: Living and Non-Living Resources
Biodiversity: Living and Non-Living Resources
 
Traditional versus Modern Biotechnology (Exam 2 coverage)
Traditional versus Modern Biotechnology (Exam 2 coverage)Traditional versus Modern Biotechnology (Exam 2 coverage)
Traditional versus Modern Biotechnology (Exam 2 coverage)
 
BIODIVERSITY: definition, levels and threats
BIODIVERSITY: definition, levels and threatsBIODIVERSITY: definition, levels and threats
BIODIVERSITY: definition, levels and threats
 
Classical Biotechnology: FERMENTATION
Classical Biotechnology: FERMENTATIONClassical Biotechnology: FERMENTATION
Classical Biotechnology: FERMENTATION
 
Answers to groupie_for my MBB students
Answers to groupie_for my MBB studentsAnswers to groupie_for my MBB students
Answers to groupie_for my MBB students
 
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
Lecture on DNA to Proteins (The Central Dogma of Molecular Biology)
 
Lecture on nucleic acid and proteins
Lecture on nucleic acid and proteinsLecture on nucleic acid and proteins
Lecture on nucleic acid and proteins
 
Material Cycling lecture
Material Cycling lectureMaterial Cycling lecture
Material Cycling lecture
 
Mendelian genetics lecture quiz
Mendelian genetics lecture quizMendelian genetics lecture quiz
Mendelian genetics lecture quiz
 
Ecology and Ecosystem Concepts
Ecology and Ecosystem ConceptsEcology and Ecosystem Concepts
Ecology and Ecosystem Concepts
 
Chemistry of life and Metabolism
Chemistry of life and MetabolismChemistry of life and Metabolism
Chemistry of life and Metabolism
 
NS5 Lecture 1: Environmental Ethics
NS5 Lecture 1: Environmental EthicsNS5 Lecture 1: Environmental Ethics
NS5 Lecture 1: Environmental Ethics
 
MMB1 Lecture 1: Introduction to Biotechnology
MMB1 Lecture 1: Introduction to BiotechnologyMMB1 Lecture 1: Introduction to Biotechnology
MMB1 Lecture 1: Introduction to Biotechnology
 
Mmb1 lec2 qb2013
Mmb1 lec2 qb2013Mmb1 lec2 qb2013
Mmb1 lec2 qb2013
 
Mbb lec1 qb
Mbb lec1 qbMbb lec1 qb
Mbb lec1 qb
 
Bio 151 lectures for examination 2
Bio 151 lectures for examination 2Bio 151 lectures for examination 2
Bio 151 lectures for examination 2
 
Microbial control lecture reference
Microbial control lecture referenceMicrobial control lecture reference
Microbial control lecture reference
 
Biology 120 lectures for 2nd exam 2012 2012
Biology 120 lectures for 2nd exam 2012 2012Biology 120 lectures for 2nd exam 2012 2012
Biology 120 lectures for 2nd exam 2012 2012
 
Bio 196 fbyz calendar t3 4
Bio 196 fbyz calendar t3 4Bio 196 fbyz calendar t3 4
Bio 196 fbyz calendar t3 4
 
Bioweek talk 2012
Bioweek talk 2012Bioweek talk 2012
Bioweek talk 2012
 

Biology 199 Lecture 2 (Research Process)

  • 1. Research, The Research Process, Research Methodology LECTURE 2 (BIOLOGY 199) MARILEN M. PARUNGAO
  • 2. RESEARCH viewed as a problem-solving activity follows a logical series of steps (research process) which makes it different from other problem-solving activities
  • 3. Research Methodology (Leedy, 1997) the core concept underlying the research process the methodology controls the study itself and the processes needed to realize the study the methodology controls and dictates the acquisition of data, arranges them in logical relationships, sets up a means of refining the raw data, contrives an approach so that meanings that lie below the surface of those data become manifest, and finally issues a conclusion or series of conclusions that lead to an expansion of knowledge the entire process is a unified effort as well as an appreciation of its component parts
  • 4. The Process of Research: Logical Steps THE RESEARCH PROBLEM PROBLEM IDENTIFICATION (LOOKING FOR A TOPIC) CHARACTERISTICS OF A GOOD RESEARCH PROBLEM RESEARCHABILITY OF THE PROBLEM FORMULATION OF RESEARCH OBJECTIVES DEFINITION OF RESEARCH OBJECTIVES CHARACTERISTICS OF RESEARCH OBJECTIVES
  • 5. THE RESEARCH PROBLEM THE HEART OF THE RESEARCH PROJECT REQUIREMENT: TO STATE THE PROBLEM WITH UNWAVERING CLARITY, PRECISION WHAT IF I SIMPLY CANNOT FIND A GOOD PROBLEM?
  • 6. IDENTIFICATION OF THE PROBLEM WHERE TO FIND INTERESTING PROBLEMS... JOURNALS, BOOKS, ABSTRACTS (LIBRARY/TRUSTED LINKS) RECOMMENDATION SECTIONS OF THESES AND DISSERTATIONS/JOURNAL ARTICLES IDEAS FROM YOUR MENTOR OR PROFESSOR IDEAS FROM SEMINARS, RESEARCH COLLOQUIA AND CONFERENCES PERSONAL/FAMILY EXPERIENCES RARE/INTERESTING OCCURRENCES WHICH NEEDS TO BE EXPLAINED TOP TEN CAUSES OF MORTALITY/MORBIDITY IN YOUR LOCALITY
  • 7. CHARACTERISTIC OF A RESEARCH PROBLEM SHOULD BE OF GREAT INTEREST TO YOU USEFUL FOR THE CONCERNED PEOPLE IN A PARTICULAR FIELD POSSESS NOVELTY LAYS FOUNDATION FOR FURTHER RESEARCH IN THE FIELD CAN BE COMPLETED IN THE ALLOTTED TIME DESIRED MUST USE APPROPRIATE AND UP-TO-DATE TECHNOOLOGY DOES NOT CARRY ETHICAL OR MORAL IMPEDIMENTS
  • 8. A GOOD RESEARCH PROBLEM SHOULD BE SMART SPECIFIC, MEASURABLE, ACHIEVABLE, REALISTIC, TIME-BOUND
  • 9. IS MY PROBLEM WORTHY OF RESEARCH? EXTERNAL FACTORS NOVELTY AND AVOIDANCE OF UNNECESSARY REPETITION PRACTICAL VALUE OF THE PROBLEM
  • 10. IS MY PROBLEM WORTHY OF RESEARCH? PERSONAL FACTORS TRAINING AND PERSONAL QUALIFICATIONS TIME REQUIREMENTS AVAILABILITY OF SUBJECTS AND EQUIPMENTS SPECIALIZED WORKING CONDITIONS HAZARDS TO BE ENCOUNTERED RESEARCH FUNDS (COST)
  • 11. STATING YOUR RESEARCH PROBLEM THE RESEARCH PROBLEM MUST BE STATED IN A CLEAR AND COMPLETE GRAMMATICAL SENTENCE IN AS FEW WORDS AS POSSIBLE!
  • 12. WHAT’S WRONG WITH THESE RESEARCH BUSING OF SCHOOL CHILDREN RETIREMENT PLANS OF ADULTS EFFECT OF PHARMACEUTICALS ON EMBRYO E. COLI AND WATER QUALITY
  • 13. FORMULATION OF RESEARCH OBJECTIVES RESEARCH OBJECTIVES REFLECT THE QUESTIONS WHOSE ANSWERS THE INVESTIGATOR WANTS TO STUDY YIELD TO CAN BE EXPRESSED EITHER IN THE FORM OF A STATEMENT OR A QUESTION SERVES AS THE STEERING WHEEL IN THE CONDUCT OF A RESEARCH PROJECT SERVES A S AGUIDE IN SPECIFYING VARIABLES TILL INTERPRETATION OF RESULTS
  • 14. SAMPLE OBJECTIVES TO DEVELOP AN OPTIMIZED PROTOCOL TO DETECT FLAVIVIRUSES IN SERUM SAMPLES USING PCR TO DETERMINE THE EFFICACY OF ORAL ADMINISTRATION OF PROBIOTICS IN MANAGING OBESITY TO ESTABLISH THE RELATIONSHIP BETWEEN PLANT HEIGHT AND FERTILIZER CONCENTRATION IN CORN
  • 15. IMPORTANT CHARACTERISTICS OF RESEARCH OBJECTIVES PHRASED CLEARLY, UNAMBIGUOUSLY AND SPECIFICALLY STATED IN MEASURABLE TERMS AND DO NOT INVOLVE VALUE JUDGEMENT
  • 16. GENERAL VERSUS SPECIFIC GENERAL OBJECTIVE a generic statement which describes in broad terms what the study wishes to accomplish SPECIFIC OBJECTIVE contain indicators on how to accomplish the stated objectives and therefore, gives direction to the research process; identifies in detail and measureable terms the aims of the research study
  • 17. EXAMPLE General Objectives: To investigate the histological effects of neem seed kernel extract on mouse testis Specific Objectives To determine/identify the changes in testes histology due to neem seed kernel extract (NSKE) exposure To determine the relationship between neem seed kernel extract (NSKE) and occurrence of abnormal sperm morphology To provide a feasible physiological basis for the anti-libido property of neem extract
  • 18. REVIEW OF RELATED LITERATURE After the research problem has been identified and the objectives formulated, a review of related literature needs to be done. Two Important Uses: • To get acquainted with the existing studies related to the research to be conducted relative to: - who have done the work on the problem area - what has been found - research design utilized - statistical analysis applied - problem met and how were they resolved • To establish a rationale or a theoretical or conceptual framework based on previous research studies done.
  • 19. SCOPE AND LIMITATIONS Researcher must be shrewd in narrowing the scope of his study without becoming concerned with a trivial problem Assumptions, restrictions and limitation must be explicit with respect to the coverage of the study Helps focus attention on valid objectives, & helps minimize the dangers of over generalization
  • 20. FACTORS TO CONSIDER IN DELIMITING THE PROBLEM the scope of the problem time allotted for the conduct of the study cost and funding cooperation/coordination needed from other institutions or researchers availability of research subjects availability of equipment needed ethical considerations
  • 21. EXAMPLE Impact of continuing education for health workers The effect of continuing education activities conducted by the Department of Health (DOH) for its staff on their performance The effect of workshops/seminars conducted by the DOH for its staff on their ability to manage the different programs of the DOH in the field To determine the effect of the Master Trainor’s Course conducted by the DOH on the capabilities of the participants to plan, implement, monitor, and evaluate the training programs they conduct in the field
  • 22. FORMULATION OF A TESTABLE HYPOTHESIS
  • 23. DEFINITION A tentative explanation for certain phenomena, or events which have occurred or will occur (Gay, 1976) States the researcher’s expectations concerning the relationship between two or more variables in the research problem Testable statement of a potential relationship between two or more variables (McGuigan, 1978)
  • 24. CHARACTERISTICS OF A GOOD HYPOTHESIS Stated in declarative form Stated in definite terms, the relationship between variables Should reflect the theory or literature that it is based on Should be brief and to the point Should be testable
  • 25. TWO TYPES OF HYPOTHESIS “RESEARCH HYPOTHESIS AND THE NULL HYPOTHESIS”
  • 26. THE NULL HYPOTHESIS Ho Never true or established but can be possibly disproved in the course of the experimentation No difference relationship between the variables we want to study May act as a starting point and as a benchmark against which the researcher will measure the actual outcome of the study once the researcher has collected the data
  • 28. EXAMPLES Ho : Vitamin C does not inhibit chromosomal lagging HA : Vitamin C does inhibit chromosomal lagging by 50% compared to placebo Ho : Cerebral artery bypass is as effective as standard medical therapy HA : Cerebral artery bypass is more effective than standard medical therapy
  • 29. TWO TYPES OF RESEARCH HYPOTHESIS Non-directional – reflects a difference between groups, but the direction of the difference (unequal) is NOT specified Directional – reflects a difference between groups and the difference is specified
  • 30. IDENTIFICATION OF RESEARCH VARIABLES Variable – any trait/characteristic that manifest differences irrespective of whether the differences are qualitative or quantitative Qualitative – eye color, shape of teeth, sex Quantitative – weight, height, length, light intensity, temperature
  • 31. TYPES OF VARIABLE Independent – the treatment variable variables in the course of an experiment in an effort to understand the effects of this manipulation on some outcome (which you know as the dependent variable) the variable which is presumed to cause, effect, influence, or stimulate the outcome Dependent – outcome variables in a research study refers to the outcome or response variable Extraneous Variable – by themselves produce changes which may be mistaken to be the effect of the independent variable being considered Controlled, held constant or randomized – so the effects are neutralized, cancelled out or equated for all conditions
  • 32. TRY THIS... PROBLEM: the effect of carbon dioxide loading on plant morphology Identify the: Independent variable Dependent variable Intervening/extraneous variable
  • 33. CONSTRUCTION OF A RESEARCH DESIGN represents the “plan of attack” of the researcher in answering the research objectives in obtaining all the relevant data in relation to objectives and hypothesis the specific areas of concern in the choice of a research design are the following selection and number of subjects control and manipulation of relevant variables establishment of criteria to evaluate outcomes instrumentation maximization of internal and external validity
  • 34. FACTORS TO CONSIDER research objectives feasibility ethical considerations economy and efficiency internal and external validity
  • 35. INTERNAL VALIDITY refers to extent to which investigator is able to control the different biases affecting the study and in the end, measures what he really intends to measure Did the experimental treatment really bring about a change in the dependent variable? Did the independent variable make a significant difference?
  • 36. EXTERNAL VALIDITY refers to the extent to which the investigator is able to generalize the results of his study Are the results applicable to groups and environment outside of experimental setting?
  • 37. DESIGN THE TOOLS FOR DATA COLLECTION Experimentation Questionnaire Interview schedule and forms
  • 38. DESIGN THE PLAN FOR DATA ANALYSIS A number of researchers think about data analysis only after all data has been collected Consequences: Some very important variables in study are either not measured at all or collected using a measurement scale which is inconsistent with desired mode of data analysis Objectives are too ambitious or non-measurable, given the nature of the data that were collected
  • 39. THE SOLUTION... A good practice is to construct a dummy table Dummy Tables – skeleton tables drawn to help the investigator conceptualize how the data is going to be organized and presented after it has been collected
  • 40. COLLECTION OF DATA Essential phase of the research process Researcher employs specialized tools, instruments and procedures depending upon the method designed for such activity
  • 41. DATA PROCESSING Process the information gathered to prepare for and facilitate analysis and interpretation of data. Editing of data collection forms and coding of responses are procedures usually done in this stage
  • 42. DATA ANALYSIS AND INTERPRETATION Involves quantification, description, and classification of data Statistics play a major role Researcher must be familiar with basic statistical concepts and procedures and must know their limitations as well as the areas where they may be appropriately applied
  • 43. DRAWING CONCLUSIONS AND RECOMMENDATIONS Researcher summarizes the discussion on the research findings and make a clear concluding remarks Researcher identifies major points that were not raised in the present study and could lay the framework for future undertakings
  • 44. WRITING OF RESEARCH REPORT Researcher prepares report of different activities he has undertaken together with his findings Report must be well-organized and presented in proper form and style The basic principles of technical report writing are followed
  • 45. REPORT OF RESEARCH FINDINGS Publish findings in scientific journals and news releases Presentation of results in scientific meetings
  • 46. SO...READY TO DO YOUR RESEARCH???