1. ANALYSIS OF PHYTOCHEMICAL
CONSTITUENTS AND
ANTIMICROBIAL ACTIVITIES
OF ALOE VERA L. AGAINST
CLINICAL PATHOGENS
WANNURUL SYAFAWANI BT WANMOHDTAUFEK
116381
GTF 211 NATURAL PRODUCT
CHEMISTRY

2. 1. Explained about :
I ) the presence of medical active constituents in Aloe vera.
II) antibacterial activity and antifungal activity of Aloe vera in
different extracts solution (aqueous, ethanol and acetone
solution)
2. Gain a new information and new knowledge about 26 bioactive
phytochemical compounds in the ethanolic extract of Aloe vera
3. It said that Aloe vera suitable use for the treatment of various
infections including skin transmitted infections.
REASON WHY THIS ARTICLE IS CHOOSEN

3. INTRODUCTION OF THIS ARTICLE
1. The aim of the study was to investigate the Aloe vera phytochemical
compounds and antimicrobial and antifungal activity of different extracts
2. Aloe vera :
i) is a cactus like plant with green , dagger – shaped leaves that are
freshy , tapering, spiny, marginated and filled with a clear viscous
gel.
ii) has been used to treat various skin condition such as cuts, burns,
eczema, eases pain, reduces inflammation and sap on wound
healing.

4. NEW INFORMATION PROVIDED
1. This study has revealed the presence of many secondary metabolites
in the leaves of Aloe vera.
2. It has the further confirmed that the plant extracts could be used for
the treatment of various infections including skin transmitted
infections.
3. The phytochemical active compounds of Aloe vera were qualitatively
analyzed.

5. Table 1: Qualitative analysis of photochemical al components
1. By using GC-MS analysis, 26 bioactive phytochemical
compounds were identified in the ethanolic extract of Aloe Vera
based on their peak area , molecular weight and molecular
formula
Sl. No. Phytochemical components of
qualitative analysis
Aloe vera
1. Tannin +
2. Phlobatannins -
3. Saponin +
4. Flavonoids +
5. Steriods -
6. Terpenoids +

6. 1. The results lend credence to the folkloric use if this plant in treating
microbial infection and shows that Aloe Vera could be exploited for
new potent antimicrobial agents.
2. Antibacterial activity (maximum growth ) of Aloe Vera was
observed in Streptococcu spyogenes and Pseudomonas aeroginosa
when compared with Staphylococcus aerues and Escherichia coli
after all bacterial were incubated at 27C for 24hours .
3. The maximum antibacterial activities were observed in acetone
extract compare to ethanol extract and aqueous extract.
4. Specific plant compounds such as saponins been proposed to have
direct antimicrobial activity.

7. 1. The maximum antifungal activities of Aloe vera were observed
in Aspergillus flavus compare to Aspergillus niger after fungal
plates were incubated at 24C for 72hours
2. Aloe vera extracts have been shown to inhibit the growth of fungi
that cause tinea, however evidence for control beneath human
skin remains to be established.
3. The maximum antifungal activities were observed in acetone
extract compare to ethanol extract and aqueous extract.
4. Aloe vera extracts failed to show antibiotic properties against
Xanthomonas species

8. 1. Does not explain very details about phytochemical constituents and
antimicrobial activities even though this article easy to understand.
2. One of the reviews found that Aloe Vera has not been proven to offer
protection for humans from skin diseases, constipation for radiation
injury and for wound healing or burns .
THE WEAKNESS OF THE ARTICLE

9. SOME AREAS NEED FURTHER RESEARCH/
INFORMATION
1. Aloe vera need further research because we does not know whether could
the presence of phytochemical constituents be responsible for the
observed effects as are studies of the oral exposure of humans to Aloe
vera.
2. The effects of oral ingestion of Aloe vera need further research because
oral ingestion of Aloe vera may also cause diarrhea, which in turn can lead
to electrolyte imbalance, kidney dysfunction, dry mouth, headache, and
nausea.
3. Data are needed to define the clinical effectiveness of this popular herbal
remedy more precisely. One of the reviews found that Aloe Vera has not
been proven to offer protection for humans from sunburn, suntan, or other
damage from the sun.

10. 3. Well-designed trials with sufficient details of the contents of Aloe
Vera products should be carried out to determine the effectiveness
of Aloe Vera
4. This part need further research because :
I. To confirm the advantages of this plant in medical uses
II. To ensure whether this plant is really useful as a multipurpose skin
treatment due to the presence of saponin, a chemical compound
that acts as an antimicrobial agent

11. THANK YOU