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AMNIOCENTESIS
Presented To U By –
WHAT IS AMNIOCENTESIS
• Amniocentesis (also referred to as amniotic
fluid test or AFT) is a medical procedure used
in prenatal diagnosis of
chromosomal abnormalities and fetal
infections, and also for sex determination, in
which a small amount of amniotic fluid, which
contains fetal tissues, is sampled from
the amniotic sac surrounding a
developing fetus, and then the fetal DNA is
examined for genetic abnormalities.
WHAT IS AMNIOTIC FLUID
During pregnancy, the fetus is
surrounded by amniotic fluid, a
substance much like water. Amniotic
fluid contains live fetal cells and other
substances, such as alpha-fetoprotein
(AFP). These substances provide
important information about
your Baby’s health before birth.
WHEN IT SHOULD BE
PERFORM
• An amniocentesis, the procedure is usually
scheduled between the 15th and 18th week of
pregnancy.
• The average price for amniocentesis
is $200 to $500, and is covered by many
insurance plans.
PROCEDURE
PROCEDURE
• If used for prenatal genetic diagnosis, fetal cells
are separated from the extracted sample. The
cells are grown in a culture medium, then fixed
and stained.
• Under a microscope the chromosomes are
examined for abnormalities. The most common
abnormalities detected are Down
Synrome (trisomy 21), Edward
syndrome (trisomy 18), and Turner syndrome
(monosomy X). I
FIGURE SHOWING
ANIOCENTESIS
PROCEDURE
• In regard to the fetus, the puncture seals and
the amniotic sac replenishes the liquid over
the next 24–48 hours.
RISKS OF
AMNIOCENTESIS
There is a small risk that an
amniocentesis could cause
a miscarriage (less than 1%, or
approximately 1 in 200 to 1 in 400).
Injury to the baby or mother, infection,
and Preterm labor are other potential
complications that can occur, but are
extremely rare.
HOW SEX DETERMINATION
IS DONE THROUGH IT ??
#. Human chromosomes are 46 in number, but the last
two determine sex; they are similar, or XX, for the
female and dissimilar or XY, for the male.
#. The amniotic fluid sample, after being tapped
(known as amniocentesis), is centrifuged; then the
cell sediments are fixed in a mixture of acetic acid
and methanol, smeared on a slide and subjected to
the 'Barr body test', or a test to ascertain if the last
chromosome is an X-chromosome.
#. A positive Barr body finding establishes the foetus to
be female.
ADVANTAGE AND
DEAVANTAGES OF
AMNIOCENTESIS
ADVANTAGES
1.Confirms if abnormality is
present in fetus that may have
been detected from other tests,
such as blood test.
2. Offers a specific diagnosis of
chromosomal or genetic
abnormality prior to birth of
child.
3. Diagnosis of other hereditary
defects depending on family
history.
4. Allows early preparation for child
with birth defect or early
decision making as to carrying
the baby to full term.
DISADVANTAGES
1.Miscarriage is the main risk when
preforming Amniocentesis
(Chances are 1 in 400 caused by
possible infections in the uterus).
2. Although extremely rare, there is
always a risk the needle might hit
the baby within the fetus.
3. Following the procedure, the
mother may experience side
effects such as cramping, leaking
of fluid, and irritation around the
area of puncture
AMNIOCENTESIS IS BANNED IN
INDIA ?????
As girl child was never accepted in many
family,when a women got pregnant her family
got amniocentesis done by which they could
find the gender of the foetus and if they the
foetus was girl they would stop the baby to
come step in the earth. Seeing this
reason,amniocentesis is banned in India.
STOP FEMALE
FOETICIDE ….
DON’T KILL US
WE WANT TO LIVE !!
THANX FOR GIVING ME SUCH AN
OPPORTUNITY !!

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Amniocentesis :- Friend or Foe

  • 2. WHAT IS AMNIOCENTESIS • Amniocentesis (also referred to as amniotic fluid test or AFT) is a medical procedure used in prenatal diagnosis of chromosomal abnormalities and fetal infections, and also for sex determination, in which a small amount of amniotic fluid, which contains fetal tissues, is sampled from the amniotic sac surrounding a developing fetus, and then the fetal DNA is examined for genetic abnormalities.
  • 3. WHAT IS AMNIOTIC FLUID During pregnancy, the fetus is surrounded by amniotic fluid, a substance much like water. Amniotic fluid contains live fetal cells and other substances, such as alpha-fetoprotein (AFP). These substances provide important information about your Baby’s health before birth.
  • 4. WHEN IT SHOULD BE PERFORM • An amniocentesis, the procedure is usually scheduled between the 15th and 18th week of pregnancy. • The average price for amniocentesis is $200 to $500, and is covered by many insurance plans.
  • 6. PROCEDURE • If used for prenatal genetic diagnosis, fetal cells are separated from the extracted sample. The cells are grown in a culture medium, then fixed and stained. • Under a microscope the chromosomes are examined for abnormalities. The most common abnormalities detected are Down Synrome (trisomy 21), Edward syndrome (trisomy 18), and Turner syndrome (monosomy X). I
  • 8. PROCEDURE • In regard to the fetus, the puncture seals and the amniotic sac replenishes the liquid over the next 24–48 hours.
  • 9. RISKS OF AMNIOCENTESIS There is a small risk that an amniocentesis could cause a miscarriage (less than 1%, or approximately 1 in 200 to 1 in 400). Injury to the baby or mother, infection, and Preterm labor are other potential complications that can occur, but are extremely rare.
  • 10. HOW SEX DETERMINATION IS DONE THROUGH IT ?? #. Human chromosomes are 46 in number, but the last two determine sex; they are similar, or XX, for the female and dissimilar or XY, for the male. #. The amniotic fluid sample, after being tapped (known as amniocentesis), is centrifuged; then the cell sediments are fixed in a mixture of acetic acid and methanol, smeared on a slide and subjected to the 'Barr body test', or a test to ascertain if the last chromosome is an X-chromosome. #. A positive Barr body finding establishes the foetus to be female.
  • 11. ADVANTAGE AND DEAVANTAGES OF AMNIOCENTESIS ADVANTAGES 1.Confirms if abnormality is present in fetus that may have been detected from other tests, such as blood test. 2. Offers a specific diagnosis of chromosomal or genetic abnormality prior to birth of child. 3. Diagnosis of other hereditary defects depending on family history. 4. Allows early preparation for child with birth defect or early decision making as to carrying the baby to full term. DISADVANTAGES 1.Miscarriage is the main risk when preforming Amniocentesis (Chances are 1 in 400 caused by possible infections in the uterus). 2. Although extremely rare, there is always a risk the needle might hit the baby within the fetus. 3. Following the procedure, the mother may experience side effects such as cramping, leaking of fluid, and irritation around the area of puncture
  • 12. AMNIOCENTESIS IS BANNED IN INDIA ????? As girl child was never accepted in many family,when a women got pregnant her family got amniocentesis done by which they could find the gender of the foetus and if they the foetus was girl they would stop the baby to come step in the earth. Seeing this reason,amniocentesis is banned in India.
  • 14. DON’T KILL US WE WANT TO LIVE !!
  • 15. THANX FOR GIVING ME SUCH AN OPPORTUNITY !!