Gene electroporation, often simply referred to as electroporation, is a potent molecular biology technique employed to introduce genetic material, like plasmid
DNA, RNA, or other molecules, into specific cells. This procedure leverages the transient permeabilization of the cell membrane induced by brief electrical
pulses, facilitating the entry of external DNA or RNA into the cell. Typically, cells are cultivated and primed for electroporation, either in suspension or
adherent culture. Subsequently, the genetic material is combined with the target cells, and in some instances, cells are positioned within a cuvette or
electroporation chamber along with the genetic material.
2. Gene Electroporator is a specialized
electroporator suspended with electrical
pulses that ensure temporary pores in the cell
membrane for easy entry of genetic material
like DNA or RNA into the cell. Enhanced
with a user-friendly man-computer dialog
interface. Once the optimum condition arises
it can transfect a large number of cells in a
short time frame.
Specifications :
High
Output
Voltage
400 to 2500 V
3. Low
Output
Voltage
100 to 450 V
High
Voltage
Capacitor
1, 5, 6, 25, 30, 31 µF
Low
Voltage
Capacitor
100 µF,125 µF,150 µF….1675
µF, one grades of 25 µF
Resistanc
e
50 Ω, 100 Ω, 150 Ω, 1600 Ω,
increment total 30 grades
5. Features :
- Generates an exponential waveform
output for efficient electroporation
- Enables quick and efficient
transfection of a large quantity of cells in
a short duration
- Incorporates an intelligent network
system for real-time measurement and
control of parameters
- Allows for networking of gene
importation systems with two-way
communication functions, enhancing
data exchange and coordination
- Utilizes microprocessor-controlled
technology to ensure accurate and
consistent performance
6. - Includes safety mechanisms to prevent
electrical hazards and protect the
operator and samples
- Designed for easy cleaning and
maintenance to ensure reliability and
longevity
Applications :
It is employed for electro-perforating cells in
microorganisms, animals, and plants, and
serves roles in cell hybridization, gene
electroporation, and gene silencing.
Labmate Scientific
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