Nasty Gal Case Study

Nasty Gal Case Study
Audiences
Stakeholders can be defined as "any individual or group who can affect or be affected by the actions,
discussions, policies, practices or goals of the organization" (Freeman, 1984: 25) In the case of
Nasty Gal, their stakeholders comprise of a number of publics that they interact with and are linked
with (Esman, 1972; Grunig and Hunt, 1984:141) including current/potential customers, staff
members, investors/stockholders, women, media and political groups. According to the boohoo
website, Nasty Gal's target customer demographic is 18–27 year olds. (Boohoo Group, 2017) The
products are niche and designed for a specific demographic and young girls want to be a part of this
community.
Stakeholder Approach In its rapid growth, Nasty Gal placed an extreme focus on the needs of the
shareholders to the detriment of its stakeholders. The boohoo group is potentially making the same
mistake as by taking on the task of reviving the brand's reputation it places pressure on them to
communicate positive projected financial returns whilst simultaneous managing the expectations of
Nasty Gal's existing customer base. As a result, the company has taken the soft approach in
communicating with the existing customers of the brand by not addressing the pre–existing issues
before the acquisition which has caused consumers to react negatively online. (Racked, 2017) This
approach stems from the deal that the boohoo group made which only included the acquisition of
Nasty Gal's soft
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The Kill List Sociology
The Kill List is a British horror film, considered hybrid, coinciding with black comedy, horror
comedy, psychological horror, family drama, hitman action–thriller, war, crime thriller, cult, violent
drama.
The film incorporates violence throughout, from the first scene with domestic disharmony, to verbal
violence, continuing with actual violence, metaphysical violence with the hammer scene, violence
between Jay and Gal, and ends with Jay violently stabbing the 'Hunchback'.
As the narrative progresses, Jay sinks further into darkness. As Cherry states 'horror films are
socially reflective' (Cherry, 2010: 169) and the film comments on fears and anxieties of a
contemporary British society, the three figures on the kill list, representing institutional ... Show
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The family take on a macabre form, Shel piggybacking Sam, and Jay's stabbing them on the ground,
shown in slow motion, foreshadowing the final scene. When Jay and Gal want to abort the job, they
are told their family will be killed. With no choice, Jay continues the job however the threat to
family becomes internal at the cottage after Jay discovers his car tyres are slashed. However in
trying to protect his family from the threat, the irony is that they die at the hands of him in the final
scene.
During the argument, a medium shot shows Sam laid on his bedroom floor entertaining himself with
a toy, overlaid with sound of his parents arguing. This informs the audience that this is nothing new.
In the foreground, to the left of the frame is a toy castle, which hints at the horror genre but rather a
contemporary setting.
In contrast to the family's suburban location, the cult members are located within the landscape and
represent the wilderness and unpredictable nature. The Kill List operates 'within the subgenre of
'Urbanoia', essential elements binary oppositions – 'modern' against the 'primitive', 'present' against
the 'past' (Rose, 2009) and

BRAE 340 Problem set 1 Essay
1.
a. Power– kilowatt,
b. Area – acres,
c. Application Rate – in/hr,
d. Pressure – head,
e. Depth – inches,
f. Length – miles,
g. Velocity – ft/sec,
h. Volume – ac–in,
i. Flow Rate – cu–ft/sec
2. Statements (1) and (2) refer to water volumes. Are these Net or Gross volumes? (1) Crop water
use (cu–ft) = Seasonal ET (ft) x Crop area (acres) x 43,560 sq–ft/acre (2) Volume applied by system
(ac–in) = Application rate (in/hr) x Irrigation time (hr) x Area (acres)
a. (1) is Net; (2) is Gross
b. (1) is Gross; (2) is Gross
c. (1) is Gross; (2) is Net
d. (1) is Net; (2) is Net
3. Statements (1) and (2) refer to water amounts. Are these Net or Gross amounts? (1) Crop water ...
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A salt concentration of 1562 ppm is equivalent to an electrical conductivity of how many dS/m?
a. 1 dS/m ≈ 700 ppm
b. ppm x (1 dS/m)/(700 ppm) = dS/m
c. The correct answer is: 2.2314285714286
13. If a soil has a moisture tension of 0.15 bars and the salinity of the soil water is 1.86 dS/m, what
is the total tension experienced by the plant roots? That is, what is the sum of the moisture tension
and the osmotic (due to salinity) tension?
a. 57 cb
b. 34 cb
c. 71 cb
d. 67 cb
e. 206 cb
i. moisture tension = bars x 100 cb/bar = cb ii. osmotic tension = dS/m x 30 cb/(1 dS/m) = cb iii.
total tension = moisture tension (cb) + osmotic tension (cb)
14. What pumping rate (CFS) is required to deliver 47 acre–inches in 20 hours?
a. rate = amount ÷ time

b. rate = ac–in/hr x 1 CFS/1 ac–in/hr = CFS
c. The correct answer is: 2.35
15. Crop ET is 0.22 in/day, and irrigations are every 7 days. What is the crop water use between
irrigations (in)?
a. Amount = rate (in/day) x time (days) = inches
b. The correct answer is: 1.54
16. 30 acres of cotton uses water at the rate of 0.21 inches per day. How many cubic feet per day
does the cotton use?
a. Volume = depth (in) x area (acres) = ac–in/day
b. ac–in/day x 1 ft/12 in x 43560 sq–ft/acre = cu–ft/day
c. The correct answer is: 22869
17. A rain gauge is constructed of a funnel with diameter D = 4 inches, fixed to the top of a tube
with diameter d = 2 inches (see drawing). Rain water

Competent Cell Transformation Lab Report
By using the ligation mixture formed in experiment 3B, competent cells undergo transformation in
this experiment. The cells do not usually exist in a state of transformation ready but they can be
made permeable to the plasmid DNA and the capable of transformation are referred as competent.
Transformation is the uptake of heritable information in a competent cell. Competent cells care
extremely fragile and need to be handle gently to ensure the success of transformation. Water bath is
heated to 42oC as heat shock treatment to start transformation. The competent cells containing
recombinant vectors can be identified through blue white screening. Cells containing recombinant
vectors are able to survive on Luria Bertani (LB) medium supplemented with the antibiotic,
penicillin. The cells transformed by recombinant vectors are plating on plate that containing
isopropyl β–D–thiogalactopyranoside (IPTG) which as an inducer of the lac promoter, and 5–
bromo–4–chloro–3–indolyl– β–D–galactoside or called X–Gal which is a dye that produces a blue
colour when ... Show more content on Helpwriting.net ...
The mutant β–galactosidase is unable to form a tetramer and is inactivated. When the recombinant
vector containing N–terminal fragment of the protein, the α–peptide are expressed together with ω–
peptide, they form a functional β–galactosidase enzyme. The blue white screening works by
disrupting this α–complementation process. White colony on X–gal indicate the present of foreign
DNA fragment in lac Z gene of plasmid. Hydrolysis does not take place on X–Gal because the
insertion disrupt the formation of active β–galactosidase. In contrary, blue colony indicate the absent
of foreign DNA fragment in lac Z gene of plasmid. This is because functional β–galactosidase
enzymes are produced to hydrolysed X–Gal, create a blue

Water Footprint Research Paper
Virtual water exists in a variety of different industries beyond food–based agriculture. First off, we
could also look into beverage choices. While food is often looked into for water footprints,
beverages can also have a significant impact. If each member of the dinner party consumes one
serving of wine and one serving of beer, each person is adding 52 gallons for a total of 520 gallons
of water for those servings of beverages alone. Instead of looking to alcoholic or other high water–
footprint liquids, water itself could be consumed or an alternative such as tea where one serving is
other equal to 9 gallons of water for its footprint. One of the next industries to look into beyond
consumption completely otherwise would be transportation. Transportation has many different
factors to look into. Looking at water footprint alone, gasoline and diesel requires the smallest water
footprint of 0.55 gal/mile of withdrawal and 0.08 gal/mile of consumption. Electricity has a
withdrawal of 7.8 gal/mile and a consumption of 0.24 gal/mile signaling the highest water footprint.
Biofuels have a withdrawal of 0.49 gal/mile and a consumption of 0.30 gal/mile. Looking at this
alone, it would appear that gasoline and diesel is the best option. However, when we consider both
potential pollution, ... Show more content on Helpwriting.net ...
The water footprint for energy is substantial. The energy required to provide 100 gallons of clean
water requires 300 gallons of water to produce. Therefore, reducing energy consumption could
drastically assist in reducing water footprints. Therefore, when preparing all food during
Thanksgiving, water waste should be kept to a minimum so that energy is not wasted in distribution
and heating of this water. Additionally, electricity waste should be minimized with typical methods
such as turning off electronics that are not necessary and using alternative energy methods may
prove to be

Paul's Letter To Galatians
Paul's letter to the Galatians has been an significant piece of scripture to Christianity throughout the
ages. Not only has it set the foundation for different formations within the Christian religion, but it
describes the importance of faith in Jesus Christ. Having faith in Christ is Paul's main point within
the letter to the Galatians. Two biblical scholars, John R.W. Stott and Krister Stendhal have analyzed
Paul and his writings. Both authors have good viewpoints and understanding of what Paul is
explaining, but it is Stendhal who seems to have a better grasp on the true meaning behind Paul's
letter to the Galatians. Galatians 6:14–15 is a fantastic conclusion to Galatians 1–3 because it is
evident Paul makes his argument through justification in faith alone, rather than through the Law. ...
Faith in Christ is principal when becoming a Christian, while the Torah, can be neglected. That is
why Galatians 6:14–15 is a great conclusion for Galatians 1–3. Paul writes "through faith in Jesus
Christ the promise might be given to those who believe" (Gal 3: 22). To Paul, the only vital thing is
Christ and believing in his revelation. Having this understanding allows those who believe to live a
more righteous life through the cross, which is another word for faith. That is why when Paul writes
"I never boast except in the cross of our Lord Jesus Christ" (Gal 6:14–15), he is justified to do so
because of his faith. If Paul still saw the Torah as his main way of salvation, then he could not boast
in the

The Discovery of Transformation by Frederick Grifith in...
The discovery of transformation by Frederick Griffith in Streptococcus pneumonia has played an
important role in how we are now able to introduce plasmid DNA molecules into cells.
Transformation is the uptake of DNA molecules released from the donor cell by the recipient cell. It
is one of the three ways bacteria are able to exchange genetic material. In Griffith's experiment he
introduced mice to two different forms of S. pneumonia, one smooth, pathogenic and encapsulated
and the other rough, nonpathogenic and noncapsulated (Snustad, 193). The mice were injected with
live rough strain and heat killed smooth strain. The deaths of the mice lead Griffith to conclude that
some genes of the killed smooth strain were transformed to the rough strain and the bacteria became
encapsulated and pathogenic, therefore leading to the death of the mice (Snustad, 193).
Plasmids are small circular DNA molecules. They are not essential for survival of the host bacteria.
Some carry genes that allow resistance to antibiotics (Anderson). Plasmid pUC18 is a circular DNA
molecule. It contains portions of the E. coli Lac Z gene, which encodes for the first 146 amino acids
of β – galactosidase. E. coli contains the Lac Z gene, which encodes β – galactosidase . The E. coli
Lac operon digests lactose. Once E. coli is transformed with pUC18, complementation occurs. E.
coli produces active β –galactoidase. The active β –galactoidase hydrolyzes the substrate, X gal ,
which is located on the agar plates.

P53 Protein Case Study
In the spot assay, p53 mutant T155N was tested for binding by the color it develops. On the high
adenine agar plate, the p53 strains, wild type (wt), mutant T155N (mut), and deletion (del),
produced white cultures with all p53 response element (RE) sites, consensus (con), p21, and BAX.
Therefore, all p53 strains showed transactivation because there was already enough ADE2 protein
on the plate (see Figure 1B). Unlike the high adenine agar plate, the low adenine agar plate showed
significant differences between the p53 strains and the p53 REs. The p53 wt strain developed white
cultures while the p53 deletion strain developed red cultures. The p53 mut strain developed a pink
culture with p53 RE con and red cultures with p53 RE p21 and BAX. In this case, p53 wt shows
transactivation and a high binding affinity with all three p53 REs. Contrarily, the p53 del strain
shows no transactivation and binding affinity with all the p53 ... Show more content on
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If there is no protein activating the response element, then there is no activation of ADE2 to make
ADE2 protein. This causes a buildup of AIR during the AIR to CAIR process, and with the addition
of oxygen, the AIR turns the culture a red color. The culture develops into a pinkish color because
the protein is not binding fully to the response element, and therefore does not transactivate the
ADE2 protein completely. With some ADE2 protein in the AIR to CAIR process, there is less of a
buildup of AIR, but still enough where oxygen interacts with it to create a pinkish color (Aronson
and Silveira, 2009). Therefore, based on our results, p53 T155N partially binds with p53 RE with
consensus and does not bind at all with p53 RE with p21 and BAX. P53 T155N shows different
binding affinities with each of the p53 RE. The mutant binds the most to p53 RE con which has the
highest binding affinity of all the p53

Child Abuse And Neglect Of Children
Patricia Ivory Instructor Dr. Andrew Freeman GENE 100: To Make Abused and Neglected Children
Whole 3 May 2017
Ivory 1
] To Make Abused and Neglected Children Whole
Introduction
The abuse and neglect of children becoming victims in homes is a national epidemic.
Because of its severity and being so widespread, there are organizations set up by the juvenile
judicial court system that assign Court Appointed Special Advocates (CASA) as volunteers to
intervene in the families of reported physical/sexually abused and neglected children; that they may
experience childhood with all of its innocent privileges without the hurt, pain, and neglect.
The children come from homes of natural parents, single parents, grandparents, foster, and/or
court ... Show more content on Helpwriting.net ...
The type of abuses known to children are: child sexual abuse, physical child abuse, emotional child
abuse, neglect of a child, domestic violence, and shaken baby syndrome We know that of late, child
trafficking, which is the exploitation of children under 18 years of age is getting worse. "The US
Department of Justice has supported CASA advocacy since 1985 through its Office of Juvenile
Justice and Delinquency Prevention (OJJDP), it has changed the lives of many"
(www.casaforchildren.org). Neglect has proven to be the most severe form of abuse in the home
outweighing the physical abuse.
"In 2014 more than 76,000 CASA and guardian ad litem (GAL) volunteers helped more than
251,000 abused and neglected children find safe, permanent homes. CASA/GAL volunteers are
everyday citizens who have undergone screening and training with their local CASA/GAL program"
(www.casaforchildren.org). The Guardian Ad Litem (attorneys) are also appointed by the judges –
some are paid and others are volunteers.
Cherish Character
What type of people are these volunteers that stand in the gap for this culture of abused children?
People that have been empowered by the courts to bring support, love and peace to the children that
need it with a passion to help them that are hurting. They not only bring wonderful change to the
lives of these defenseless children, their lives are enriched as well. The cherishing of character is
aligned with the type of volunteers for

Gene Expression Lab Report
Through multiple experiments, promoter elements were examined to see how they control gene
expression. The purpose was to learn how a gene is regulated in bacteria, how timing affects
activity, how positive and negative regulation control gene expression, how E. coli mutants respond
in plate assays, and how concentrations of sucrose affects gene expression. ß–galactosidase enzyme,
also known as Beta–gal or ß–gal, was the assay in the experiments. ß–galactosidase is an enzyme
that breaks up lactose into galactose and glucose. Furthermore, it is a hydrolase enzyme that
catalyzes the hydrolysis of ß–galactosides into monosaccharides.
E. coli is often used in biology labs and is easy to manipulate. "E. coli are rod shaped bacteria that
are found ... Show more content on Helpwriting.net ...
Once lab began, the IPTG was added to the time 0 samples cultures, a final concentration of 1mM
was added to both cultures, and the timer was started. Every 30 minutes over a time period of 90
minutes 2ml was removed from each culture and the initial flask was returned to the 37°
environment to continue growing. The 2ml was equally divided into two different test tubes. The
tubes were labeled A and B. There was four tubes at each time point; 2 for lacZ+ and 2 for lacZ–.
Tube A was the control tube, which contained .2ml of water. Tube B was the experimental tube,
which contained .2ml of water and .013M ONPG. The tubes were then incubated in 37° bath water
for 20 minutes. After 20 minutes elapsed the tubes were removed and 2.7ml of 1M Na2CO3 was
added to each tube. Next 1ml was transferred from each tube into a cuvette, which was placed in the
spectrophotometer at 420nm. A spectrophotometer, also known as a spec, reads the intensity of light
(absorbance level of liquids). Tube A was inserted first in order to blank the spec, and tube B was
second, so we could record the absorbance of the solution. The absorbance was recorded for each
cuvette containing a solution from tube B. The A420 recordings we were needed to determine ONP
produced in mmol and the activity. The formula for activity is ONP produced divided

The Market And Economic Conditions For Buying And...
Introduction
Interested in near future business ideas, Cousin Edgar is considering buying two gasoline (gas)
stations. Gasoline is a fuel that, he believes, most American consumers will continue to use for the
foreseeable future and anticipates that the gasoline prices will continue moderately stable. The
business planning will evaluate the market and economic conditions for buying and operating the
two gas stations. The evaluation will be inclusive of Edgars' expectation of his gas stations' basic
economic conditions such as supply and demand, elasticity, costs of production, pricing, and normal
economic profit/loss. Cousin Edgar does believe that the opportunity for a healthy profit on the sale
of convenience items at each station could complement the gas stations' business profits.
Determinants of Demand
There is a demand for the availability of gas stations that offer not only fuel retailing but also food
items for the consumers' convenience. Edgar is aware that his retail gas station business could offer
complementary items to enhance the gas stations' revenues. Preliminary review of this business idea
recognized that the national average of fuel prices has been declining since the end of 2014,
http://www.fuelgaugereport.com/.
The determinants for gasoline are transportation needs, income, and consumers' lifestyles.
Transportation in most U.S. cities use gasoline–fueled vehicles and few have alternatives in fueling.
An income level is another determinant that affects

Yoko Ono Gal An Art Analysis
The "bag piece" (See figure 2) is another artwork in the corner of the whole exhibition. It is
composed by a low platform, which is white; and a large black bag on the top of it. The visitors are
allowed to crawl into the bag and do some movement in it, like roll on the ground of do some
postures. Most of the people feel that they enjoy doing something in it and in front of many viewers
because they are covered. Also, when someone is in the bag, they can see through the bag a little bit,
but the viewers cannot see anything from the outside. The bag is very soft and cozy, witch makes
people feel comfort and do not want to come out.[footnoteRef:11] [11: Blake Gopnik, "Art Critic
Gets Trapped in Bag at MoMA's Yoko Ono Gala", may 15, 2015, Accessed ... Show more content
on Helpwriting.net ...
In the "Cut Piece", the people who cut her clothes more and more are the viewers. Maybe at the
beginning of the show, they did not even realize that they would do this. Maybe the one who finally
cut off her bra straps was very shy and had trouble to go on the stage to cut a tiny piece of her dress.
But people changed eventually. Those changes are unconscious and inevitable, which exactly
exposed the essence of the unbalance between man and woman during the 1960s. People cannot
deny, because that is what they did, it is sure and real, it is not an arranged performance but a real
one with an actual reaction. This kind of form can give a bigger impact on the audience and touch
them deeply. They would really think about the social issue and make some changes from

Beta Galactosidase Lab Report
Different techniques beta–galactosidase activity can be regulated
Beta galactosidase ca be regulated by use different techniques. There can be the addition of an
inducer IPTG that continuously induce the expression of lacZ gene over time. It can also be through
manipulating the promoter regulatory protein or by addition of different monosaccharide that are
preferred over others. Beta galactosidase enzyme it binds to the lacZ gene repressor, this creates a
positive feedback because in promotes the amount of this enzyme that can be in a cell. when X gal
(5–bromo–4–choro–3–indol–beta–D galactoside) is hydrolyzed by beta galactosidase galactose is
produced and a blue color is formed. E. coli is the most preferred bacteria is performing ... Show
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A wildtype and lacZ– gene were used. This experiment involved checking different time point for
the different types of strains. For the wildtype graph, as the time point increased the ONP produced
decreased and also the beta galactosidase activity decreased. For the mutant lacZ gene when the
time point increased the enzyme activity and the ONP produced also increased but it got to a time
point of 135 minutes where there was no ONP produced, this indicted that there was no enzyme
activity. This result indicates that after a long time the inducer IPTG that was being used to continue
the enzyme activity was consumed and therefore beta galactosidase was not produced. In the second
part of the beta galactosidase where different promoter element in different condition were being
used to show the differences in the beta galactosidase induction. This part also show how promoter
interact with the inducer and the repressor. In the first part where an inducer was added, the more
IPTG added the higher the amount of ONP produced this indicate there was also higher activity of
the beta galactosidase enzyme. This indicate that IPTG is a good gene expression inducer, also the
untested solution had the lowers enzyme activity and less ONP produced. When no inducer added
just a repressor they were low enzyme activity, also when glucose was added, and no inducer little
expression is indicated. This indicates that a promoter responds negatively when there no inducer
and positively when an inducer is present. A lot of glucose cause a cAMP–CAP complex which
causes little expressions of the lacZ gene, therefore there is little ONP produced. For the X gal plate
strain 1 and 2 and 3 shows that beta galactosidase cleaved to the X gal because of the deep blue
color showing on the colonies. For the X gal + IPTG plate, only strain 1 and 2 have a blue

CASA/GAL Volunteer Cases
Court Appointed Special Advocates and Guardian Ad Litem's are just regular people who are doing
their best to make a difference in the life of a child who is involved in the court system because they
have been declared by the court to be neglected, abused or dependent. The CASA/GAL volunteer is
required to go through extensive training before they are ever given a case to handle. They are
expected to commit to at least 15 hours a month per a case and to handle 1–2 cases at a time. The
cases can last up to two years and sometimes can last longer than that. In the beginning they may put
in more hours because of all the court hearings and meeting they need to attend. The Judge who is
overseeing the case is the one who appoints a CASA/GAL volunteer. There are not enough
CASA/GAL volunteers to handle all cases at this time so they are usually used in just the most
difficult cases. The CASA program was first started in Seattle in 1977 and now has over 75,000
volunteers. There are around 1,000 CASA programs at this time in 49 different states but not in
every county in those states. Unfortunately, that is nowhere near enough volunteers to cover all the
children who would benefit from having a CASA/GAL involved in their lives. ... Show more
content on Helpwriting.net ...
Abuse is defined as any injury, physical or mental, that is not caused by an accident. Neglect is
defined as a child/children whose parents have abandoned them or who are not providing the basic
care a child needs. Dependency is defined as a child/children whose parents have ignored, where not
able to provide or denied basic care to the point the state is asking for custody of the

The Domestication of Animals Was the Percursor to Genetic...
The domestication of animals 30,000 years ago was the precursor to Genetic Engineering. Starting
with the dog we have since been able to introduce desirable traits in all organisms. With the
discovery of plasmids in the late 60's we have been able to take genetic engineering even further.
Plasmids are small circular DNA molecules used to amplify and replicate a gene of interest. These
minute molecules have the ability to replicate with the chromosome or independently, allowing them
to have up to a 100 copies in one cell. Plasmids are important because of their characteristics to
transfer genes that occur naturally within them or acting as a vector to introduce foreign DNA into a
host cell. With the use of plasmids genetic engineers are able to use bacterial transformation to make
medicines. Bacterial transformation is the process in which a bacterial cell takes up foreign DNA
and incorporates this DNA into its own. With the use bacterial transformation this part of Genetic
Engineering has become the most important and widely used technique, creating life saving
antibiotics and medicines. E.coli is generally used in these procedures because of its ability to adapt
and grow exponentially. Genetic Engineers can then use large bacterial colonies, Ampicillin, and X–
gal to indicate if b–glactosidase is present along with identifying the recombinant and non–
recombinant colonies. The transformed bacteria that contains ampicillin will spread and survive
either turning white

Special Advocate Website Analysis
This website is about the Court Appointed Special Advocate which is a program that protects the
children's rights. According to the Court Appointed Social Advocates (2017), "With a Court
Appointed Special Advocate (CASA) or guardian ad litem (GAL) volunteer dedicated to their case,
America's most vulnerable children will have someone speaking up for their best interests" (par. 1).
In addition, CASA has grown to include 800 programs nationwide. In my opinion, the program is
very beneficial to the children and their families. The need for greater involvement of child
advocates to help children have due process in the juvenile justice system is greater than ever
before. The roles that developed to provide this function are guardians ad litem (GAL)

Nasty Gal
Many of Nasty Gal's layoff and job misplacement issues could have been avoided by simply
implementing better hiring practices. In particular, three major issues Nasty Gal is experiencing,
their massing turnover, the poor fit of the CEO for her position, and the hiring of entry level workers
from other companies for managerial positions, could have been and can be mitigated via proper
hiring techniques. A simple place to start with regard to improving Nasty Gal's hiring is to promote
from internal labor markets for upper level positions instead of hiring from outside firms. First and
foremost, there is a fair amount of evidence that promoting internally is significant more efficient
than hiring from outside the firm. According to a study done ... Show more content on
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Traditionally, performance reviews involve having a manager and employee sit down on an annual
or quarterly basis to review what the employee has accomplished, agree on an overall evaluation of
the employee's performance, and discuss future plans for employee developments. Nasty Gal should
eliminate the second part of this process. Managers and employees should only discuss what the
employee has accomplished and what to focus on going forward. While information on employee
overall performance is used during subsequent talent review meetings between managers to discuss
which employees in the company are contributing the most to the organization and company values.
This approach removes the anxiety and distraction created by including an overall manager rating in
the performance review conversation, which, in return, could increase worker's

Hebrew Epistle: The Hebrew Christians Living In Rome
The Hebrew epistle is addressed to the Hebrew Christians living in Rome (Jensen, 408, Heb. 5:11–
12). The intense warnings contained in the epistle indicate that the Hebrew Christians were in a
backslidden state, in danger of apostatizing from Christ and returning to Judaism (Jensen, p. 408).
Specifically, the Hebrew Christians' faith, enthusiasm, and convictions were fading and they
neglected prayer, public worship, and the Scriptures (Heb. 3:6, Jensen, p. 408). The Hebrew epistle
is addressed to the Hebrew Christians living in Rome (Jensen, 408, Heb. 5:11–12). The intense
warnings contained in the epistle indicate that the Hebrew Christians were in a backslidden state, in
danger of apostatizing from Christ and returning to Judaism (Jensen, p. 408). Specifically, the
Hebrew Christians' faith, enthusiasm, and convictions were fading and they neglected prayer, public
worship, and the Scriptures (Heb. 3:6, Jensen, p. 408).
As a result, the Hebrew Christians conditions warranted the warning passages to teach them
appropriate behavior and avoid violation. They were informed of God's just recompense for sinning
against Him (Jensen, p. 409). The Hebrew Christians were suffering with spiritual lethargy and
disobedience, so this ... Show more content on Helpwriting.net ...
Inspired by the Holy Spirit, Paul records in Romans 5:17–21 that by the offence and disobedience of
one man (Adam) death reigned and many were made sinners; therefore, judgment came to all
humanity into condemnation,but much more they that receive the abundance of grace, and the gift of
righteousness shall reign in life by one: Jesus Christ. Jesus Christ's righteousness provided the free
gift of justification of life to all humanity through His obedience.The Law strengthened sin to
abound, but where sin abounded, God's grace abounded even the more; so the results of sin is death,
but God's grace reigns through righteousness unto eternal life by Jesus Christ Our

Biblical Essay: Analysis of Paul's Letter To The Galatians
Biblical Essay: Analysis of Paul's Letter To The Galatians
When Paul attended the Jerusalem Conference in 48 or 49, a decision was made that gentiles would
be allowed to become Christians without becoming Jews first (ie. have a circumcision, and follow
the Jewish Laws).
Paul, being the one that defended the gentile's right to be Christians, became the apostle to the
gentiles. Why would Paul, a Jew, want to be an apostle to gentiles? According to him, Jesus
appeared to him in AD 32 or
36, and told him to preach the good news to the gentiles (Gal 1:16). Paul uses scripture to explain
why gentiles should not be required to be circumcised, or obey Jewish Law; however, there are no
direct quotes in scripture that say ... Show more content on Helpwriting.net ...
So, the letter to
Galatians uses 4 specific tactics to make Galatians come back to the teachings according to Paul.
Paul begins by defending his credibility as an apostle. He writes a brief autobiographical history,
stressing that he once persecuted
Christians, and then converted when Jesus appeared to him. Also, he tells the outcome of the
Jerusalem Conference, probably to convince them that other apostles have accepted his theology.
This part of the letter is a bit like a resume of qualifications. I could imagine that the Judiazers who
came to Galatia after Paul, denounced him as an apostle: that he never met Jesus, and was not truly
educated to be an apostle.
Next, Paul writes that "obedience to the Law could not earn approval by
God; approval is possible only through faith in Christ" (Perrin, pg. 184).
Faith in the crucified Christ will bring righteousness, not the Law (Gal
2:21). Having circumcision will do nothing to make one better in the eyes of God.
Then, Paul uses an allegory of The Two Covenants: Abraham's child of a slave woman represents
Jerusalem living under the Law, and the child of the free woman represents Jerusalem being free!
This tactic, along with
Paul's use of familiar Jewish argument style, quoting scripture after scripture to prove a point (Gal
3), are common preaching styles; probably taught to him during whatever

Pglo Transformation Lab Report
Lab Report on pGAL Transformation
In order to understand this lab the student first needs to understand how recombinant DNA is
formed. To begin, the student extracts a plasmid, which is a circular strand of DNA found within
bacterial cells, from the bacteria. Restriction enzymes begin to cut the plasmid at certain sequences
of nitrogenous bases. The enzyme also cuts above and below the gene of interest and then ligase
bonds the plasmid to the gene of interest and you now have a strand of recombinant DNA. Such a
strand is useful because scientists can import useful genes into bacteria and these can be used to
create such substances such as insulin. This makes life much easier for diabetics who cannot
produce sufficient quantities on their own. On the opposing view, people may not accept this way of
producing proteins as natural due to the fact that the DNA was genetically engineered. Eventually
recombinant DNA will become high in demand because our bodies will adapt to their supply of
proteins.
Transformation is when you give organisms new traits, like ampicillin resistance, by the process ...
Another error that occurred in this lab is we ran out of time when we were supposed to incubate the
cells for 30 minutes in a 37 degree Celsius water bath. Therefore, the cells only ended up being in
the 37 degree water for about 15 minutes. Furthermore, we did not streak the plate of agar to the
best of our abilities. We were rushing through the streaking process and were not very careful with
the loop.The uncontrolled variables in this lab included one of the agar sheets in the dish had lumps
of unmelted agar on it. Another uncontrolled variable would be how much and how many of the
bacteria grew because we had no control on whether the bacteria would grow due to the fact of
mistakes that would have happened in this

Scientific Experiment: Bacterial Transformation
Title:
Research Question:
Can modifying the genetic material of bacteria create new characteristics through gene transfer?
Introduction:
Prokaryotic cells have no nucleus and no internal membrane. Because of this, they can be classified
as bacteria. Prokaryotic cells are similar in structure to Eukaryotic cells, however the DNA floats in
a nucleoid. Transformed bacteria consist of a recombinant plasmid and a host cell or bacteria. If the
plasmid in the bacteria has a gene that is useful it can be very beneficial to the bacteria. Scientists
can combine new DNA that they want replicated with the plasmid, so they can have large amounts
of it. The bacteria may pass on the plasmid onto another bacteria, creating many lawns or colonies,
which ... Show more content on Helpwriting.net ...
3.Place both tubes on ice for 15 minutes.
4.Add isolated colonies of E.Coli to the +plasmid tube using an inoculating loop. Make sure
colonies are at the bottom.
5.Repeat step 4, but with the –plasmid tube.
6.Return both tubes to ice.
7. Using a new inoculating loop, add one loop–ful of plasmid DNA to the +plasmid tube, mix it in
the tube, and return it to ice for 15 minutes.
8. During the incubation, label 6 agar plates the following: a. +Plasmid b. –Plasmid c.
+Plasmid/ampicillin d. –Plasmid/ampicillin e. +Plasmid/ampicillin/x–gal d. –Plasmid/ampicillin/x–
gal
9. After the incubation, heat shock the cells. Do this by removing the tubes directly from the ice into
42degree Celsius water bath for 90 seconds.
10. Return both tubes to the ice for approximately 1 minute.
11.Using a sterile pipette, add 250 µL of Luria Broth to each tube and mix by tapping the tubes.
Leave the tubes on a tube rack at room temperature for 10 minutes.
12.Remove 100 µL of cells from the tube and spread them on the plates with their corresponding
plasmid. (i.e. tubes with +plasmid go to the +plasmid agar plates and vice versa.)
13. Let the plates rest for several minutes to allow the cell suspensions to become absorbed into the
agar.
14. Use a sterile pipette to add 100 µL of cell suspension from the +plasmid to each appropriate
plate, and spread.
15.Seal the plates with tape, and incubate upside down for 24–36 hours at 37 degrees Celsius.

Predictions:
I predict that the plates

A Brief Summary Of Nasty Gal
As a young girl sophia was raised by the Greek Orthodox church. After being diagnosed with
depression and attention deficit disorder in her adolescence, she dropped out of high school and
began homeschooling when she was young .After high school, her parents divorced she stayed with
her mother and she moved to Sacramento, california.As a young adult, amoruso lived a normal life,
hitchhiking on the west coast, dumpster diving, and stealing. After she was caught she opened a
store on ebay she called nasty gal . The first item she sold was a book she had stolen as a teenager.
Amoruso claims to have been banned from eBay in 2008 for posting hyperlinks in feedback to
customers and launched Nasty Gal as its own retail website.Her first job as a

Gal Gadot's Diana Prince
Gal Gadot's Diana Prince will be playing a major role in this year's Batman v Superman: Dawn of
Justice, but that doesn't mean that the DC superheroine will be taking things slow after her debut.
The Wonder Woman solo movie is already filming, and star Chris Pine has just shared some details
about Diana's love interest Steve Trevor as well as the 2017 film.
Pine recently spoke to Toronto Sun, where he offered some insight on the man who sweeps Wonder
Woman off her feet.
"Steve Trevor is a rogue–ish, cynical realist who's seen the awful brutish nature of modern
civilization," Pine said of his character. "He's a worldly guy, a charming guy and it's going to be a
great, fun film. There are some incredibly deep, interesting and morally relevant

Navy Gals Research Paper
Navy SEALs BUD/s training is where the hardest part of becoming a Navy SEAL happens. BUD/s
training stands for Basic Underwater Demolition/SEAL School. The number of people who drop out
of BUD/s training is larger than the amount of people that graduate. Only twenty to thirty percent of
all applicants actually graduate the course. SEALs mean sea, air, and land. There are many stages to
becoming a Navy SEAL and many ranks that can be earned during training. The ranks stand out
through all branches of the military. Navy SEALs are an elite special operations force whose
rigorous training and specialized ranks make the organization stand out above other military
branches. The Navy SEALs began on January 1, 1962. It was originally created as a special tactical
force that could sneak in and get a job gone before anyone noticed. Thier missions included beach
and hydro–reconnaissance, explosive cable and net cutting; explosive destruction of underwater
obstacles to enable major amphibious landings; limpet mine attacks, submarine operations, and the
locating and marking of mines for minesweepers. They also conducted river surveys and foreign
military training. While doing this, the SEALs' predecessors pioneered combat swimming, closed–
circuit diving, underwater demolitions, and mini–submarine operations (Navy ... Show more content
BUD/s training stands for Basic Underwater Demolition/SEAL School. The number of people who
drop out of BUD/s training is larger than the amount of people that graduate. Only twenty to thirty
percent of all applicants actually graduate the course. SEALs mean sea, air, and land. There are
many stages to becoming a Navy SEAL and many ranks that can be earned during training. The
ranks stand out through all branches of the military. Navy SEALs are an elite special operations
force whose rigorous training and specialized ranks make the organization stand out above other
military

The Maze Runner Research Paper
Sometimes when people help others they don't always get rewarded, but sometimes they do. In The
Maze Runner by James Dashner Thomas, the main character comes into the maze and has to
overcome many challenges and helps other people out and gets them out of the maze. In another
story called Catching Fire by Suzanne Collins, Katniss has already been in the Hunger Games once
and now she has to go back in. But she also starts a rebel against the government and helps other
districts be free and have more control. In the book Catching Fire, Katniss and Gal are helping
people get out of an area because the guards are burning the place and Gal saw someone getting hit
so he tackled the guard and he got out of a post. (Collins106) "I throw myself directly between the
whip and gal". This proves that Katniss cares about more than just herself because she got hit by the
whip in the face. This also proves that she doesn't want Gal to get hurt anymore and she saved him
because he could have gotten whipped to death. Also in The Maze Runner Thomas is standing in
front of the closing doors and on the other side are two members of the maze. One of them is hurt
and the other one is trying to carry him but can't and they stop. "He moved forward the squeezed
past the connecting rods the last second he stepped in the maze." ... Show more content on
Helpwriting.net ...
They got out of the maze and they are on a bus. (Dashner 374) "In deed, we are good" This proves
Thomas was helping people by getting out of the maze and other ways but he gets rewarded by
getting out of the maze himself and maybe being able to have a regular life like he once had.
Thomas also was rewarded when he help the people get out of the maze when they were stuck. He
gained their trust and was rewarded with becoming a runner which is all he wanted to be. Which
shows that in the end if you help people out or do something good you may get rewarded with
something you have wanted or

The Basic Idea Behind Cell Tracing
Summary: The basic idea behind cell tracing, as the term suggests, is observing a cell or population
of cells over time to record the changes occurring within them. Prospective lineage analysis allows
the tracing of pre–selected candidate cells to study their outcome. Whereas, retrospective lineage
analysis refers to the systematic analysis of a population of cells at the end of a study/sample
collection to determine their origin(s). The simplest method of cell tracing is by direct observation
of the cells under the microscope without incorporation of an artificial label, and is commonly
applied to study development in lower organisms. When direct observation is not feasible, the
introduction of an exogenous marker within the cells facilitates their tracing. There are several ways
to introduce an exogenous marker – either by genetic manipulations or by non–genetic means (such
as by using vital dyes). Genetic manipulation is preferred to label cells, especially in long–term
experiments, as they allow stable incorporation of the label into the cells that remains undiluted over
time. Additionally, using genetic methods permits the expression of the label to be constitutive, cell–
type specific, or inducible, thus providing greater flexibility to the researcher and allowing both
spatial and temporal control over labeling. Cell tracing using genetically introduced labels is called
"genetic tracing". In the following section, I will elaborate on the ROSA genetic tracing approach, a

Popular Musical Development And Societal Development
The Start of Tropicalismo
Popular musical development and societal development are usually parallel with one another. Artists
and musicians can change a society and have an enormous impact on how a country runs itself. A
good example of how a country can be influenced by new, innovative music would be the
Tropicalismo movement. Caetano Veloso is a Brazilian musician that started the Tropicalia
movement and created music that combined many elements of different music together, and Veloso
did this with other tropicalists. The background of Caetano Veloso, the two songs Caetano Veloso
produced, information about Tropicalia, and the background of Brazil can help us comprehend how
music has a great impact and influence on society. Tropicalia is more than just a music genre
produced in the late 1960s, but a social–political movement that radically changed the way
Brazilians saw contemporary Brazilian music.
Caetano Veloso's start as a Brazilian musician was back in 1942 when Veloso finished school in
Santa Amaro, and he moved to Salvador where he learned how to play the guitar and sing. (Veloso,
Caetano) Veloso would spend his time at bars with his sister and perform music for people's
entertainment. Veloso met Gal Costa, Gilberto Gil, and Tom Zé in 1963 where they started musical
shows as a group. (Veloso, Caetano) One of the musical shows that they started was Vila Velha
theatre with Veloso directing the theatre. In 1965, Veloso left Federal University of Bahia to go to
Rio de

E. Colo Transformation Lab Report
Lab Report 9: Protein Crystallization and DNA Ligation and Transformation of E.Coli
Badrun nessa Rahman
Lab Partner: Briana Tolbert Section 55
March 25th, 2017
Spring 2017
Objective: The purpose of today's laboratory was to introduce a foreign gene into a cell using
recombinant DNA technology by utilizing the techniques of DNA ligation and transformation of
E.coli. Data was collected in the form of agar plates in order to analyze which colonies took up the
vector alone and which colonies took up the ... Show more content on Helpwriting.net ...
We analyzed the picture of the wells for the protein crystallizations of the lysozyme. Protein
crystallization is a method used to form the protein crystals. The protein crystal that we analyzed in
our wells are of the chicken lysozyme which forms a lattice. The chicken lysozyme is of extreme
purity and had a concentration of greater than 5mg/ml in order for it to be formed into a protein
crystal. Our protein was placed in different solution and at varying degrees of temperatures to
maximize protein crystal growth. The final step after a protein crystal grew was to place it under X–
Ray light and test the crystal for

Marni Jackson's Gals And Dolls: The Moral Value Of Bad Toys
In 'Gals and Dolls: The Moral Value of 'Bad' Toys (2011), Marni Jackson argues the bizarre
misconception created by some parents with respect to gender specific toys. Jackson is an award
winning journalist, a co–host for the TVO book program 'imprint' also teaches creative writing at
Ryerson Polytechnic University. Jackson has also collaborated on theatrical productions with the
Clichettes. She is a mother and an author of family related books like; The Mother zone: Love, Sex
and Laundry in the modern family (1992) in which she discusses the effects of some parental
pedagogy. Hence, I find her well experienced and credible enough to discuss family relationships. I
understand her intentions and agree with all her claims regarding this issue. ... Show more content
The author, Marni Jackson fully delivered all her claims while she clearly discussed the
misconception surrounding gender specific toys and parental pedagogy. She conveys her claims in a
well organised strategy with coherence and logical proof. Her informal and jovial tone makes her
article refreshing and appealing to her audience. Nevertheless, her lack of citations and reference
alongside her use of advanced vocabulary weakened the effectiveness of this article. Jackson should
have chosen words that are more basic and conversant to even a lay man. She should also have
backed up her claims by referring to more legit sources or at least cited the sources of her statistics
rather than rely on just her credibility. However, Jackson successfully appealed to her audience with
a unified and entertaining article, which is why I would use it for my own

Case Study: The Mediated Settlement Agreement
This appeal arose out of the formation of two void agreements, The Mediated Settlement Agreement
(the "Mediated Agreement") and the Agreed Amended Final Judgment of Paternity (the "Agree
Final Judgment"), as stablished by well settle common and statutory law. Consequently, relevant
facts as to the formation and parties involved in the formation of such agreements and the
subsequent modification to this agreement are relevant to the outcome of this appeal.
On May 29 2013, the lower tribunal appointed a Guardian ad Litem, Lilliana Real, Esq., to "advance
the best interest of the children." (R. 243). Ms. Lilliana Real, whom now goes by the name of Lilly
Josephine Real (Bar No. 77682) (the "GAL"), heavily influenced the father into signing The ...
Consequently, the father sent an email to the Guardian expressing all his frustration because of the
clear bias attitude of the GAL against the father. On February 11, 2014, the GAL, not losing any
time nor opportunity to hurt the father in the process and committing thereby fraud on the court,
used mentioned frustration letter against the father and requested the mother's attorney to file an
emergency hearing to modify temporary order which reads as follows: "she [the GAL] is concerned
for the safety of the children. At this time the Guardian Ad Litem is recommending that the Father
not have any timesharing with the minor children until the February 26, 2014 hearing or trial on the
case." (R. 279). Moreover, the Mother's attorney filed an email sent by the guardian ad litem, which
stated in pertinent part "I am very concerned that he may hurt the children or himself in the mental
state he is in right now." (R. 271). Moreover, the GAL dared to contact the daycare where the
children were and asked the owner not to allow the Father to picked up the children without even
having been to a hearing on the matter, or having a court order to do such a thing. (R.

Eulogy For Parents
Hi my name's Mia, I'm a fun loving, happy go lucky, loyal gal that is looking to find a family that
will love me unconditionally and always stick by my side. I'm a very people friendly gal, in fact,
when you come out to the kennels to visit with all of us, I'll eagerly be waiting patiently for you to
stop in front of mine give me some attention. In fact, I would really love it if you could leash me up
and take me out into the big kennel in the back yard and spend some one on one time with me! In
my previous home I lived with a seven year old, oh how I miss him, he was my best friend, and we
did everything together. I would really love it if my next family had some children around that age
that I could run around and hang out with. I'm still

The Testament Of The New Testament
The New Testament is known as the second major part of the Christian biblical cannon. The New
Testament, unlike the Old Testament is responsible for spreading Christianity Theology to the world.
The book was written by the disciples of Jesus. When one hear of the book, one's mind think about
the apostle Paul, since he is the one responsible for a large portion of the book and is one of the most
studied out of the apostles. Paul has written: First Thessalonians, Galatians, Philemon, Romans and
many more. However, with the dates of these gospels going back to million years ago; no one can
truly attribute all the Pauline epistles to him. Titus, Ephesians, and First and Second Timothy are all
in question of the authorship being Paul or one his followers which has been seen as a common
theme in history. Paul's main purpose for writing these letters was to educate the communities about
how to obtain a relationship with the Lord Jesus Christ. There were other letters that he wrote that
were foreshadows of the apocalypse and destruction and one letter that he wrote on behalf of a slave
(Letter to Philemon). Galatians is one of those letters were he is writing to the citizens of Galatia
and trying to help the gentile Christians one again. Paul achieves this by recalling events of his past
life, preaching the gospel that was giving him to by Jesus, and by being expressive and telling the
citizens of Galatia that they have betrayed the Lord Jesus Christ by following the Jewish Laws.

The Court Of Supreme Court
IV. THE COURT ERRED IN ENFORCING A MEDIATED AGREEMENT WHICH WAS
OBTAINED HEAVILY INFLUENCED BY THE GAL WHOM COMMITTED FRAUD UPON
THE COURT CORRUPTING THE IMPARTIAL FUNCTIONS OF THE COURT, THEREBY
MAKING THE WHOLE PROCESS VOID AS A MATTER OF LAW.
A. The actions of the court's appointed Guardian ad Litem constitute fraud on the court because they
corrupt the impartial functions of the court.
In Parramore v. Parramore, 61 Fla. 701, 703 (1911), the Supreme Court of Florida affirmed the
decree of the trial court annulling the final decree of divorce due to fraud upon the court. The
defendant in Parramore tried to commit fraud upon the court to prevent his wife from fully and
fairly presenting her case in court. The defendant in Parramore "sued the complainant as being a
resident of the city of Waco, Texas, and swore in an affidavit attached to the bill of complaint that
the said complainant resided at Waco, Texas." Id at 796.
The court in the tenth circuit defined fraud on the court and other courts have adopted this
description as "fraud which is directed to the judicial machinery itself and is not fraud between the
parties or fraudulent documents, false statements or perjury . . . It is thus fraud where the court or a
member is corrupted or influenced or influence is attempted or where the judge has not performed
his judicial function –– thus where the impartial functions of the court have been directly
corrupted." Bulloch v. United States, 721 F.2d 713, 718 (10th Cir. 1983);

Culture And Subcultures Of Fashion
If you look online, there are millions of pictures of Harajuku style. Guys and girls dressing up
androgynously, or wearing the most bizzare pieces of clothing ranging from sky–high platforms to
mixing unlikely prints together. Some even wear their hair and makeup in the most avant–garde
way. Japan for a while has been displaying fashion that intrigues us and influences us in many ways
and what people don't know is that of these fashions there are different communities and subcultures
of fashion that these people in the pictures conform to. Japan has a vast array of street fashion styles
and trends. They are all mixed with elements of traditional, foreign and/or local styles. Their street
fashion cultures vary from the tan skinned Ganguro girls to the victorian styled Lolitas. One of the
most popular and biggest Japanese fashion influencers were the high school girls of Kogyaru. What
exactly did these schoolgirls do to become one of the biggest trendsetters of Japan? The answers are
within their signature style, Japan's popular magazines, and ability to popularize certain products.
Kogyaru style was started by and became very popular among high school girls. That's what the
name literally means: 'ko' means little but it could also stand for high school and 'gyaru' means girl.
To achieve their style you must have a high school outfit with "the pleated skirts hitched up to
alarming heights" according to Richard Lloyd Parry from the Independent (Parry, par.2). Their
school

Nasty Gal Essay
Oprah Winfrey, Zig Ziglar, Richard Branson and Mark Cuban. These are all serial entrepreneurs
with serious successes. Society has become accustomed to their infinite and influential wisdom and
success and now new generations of entrepreneurs are on the rise and show no signs of slowing
down. Sophia Amoruso founder and CEO of Nasty Gal is one of these empowering young
entrepreneurs. Amoruso launched her business in the basement of her aunt's home at the age of 22
and in only seven years, Amoruso has grown Nasty Gal from a simple eBay store reselling vintage
finds into an online retail empire selling sexy, retro–inspired looks at reasonable prices. Amoruso
built this $250 million online retail empire all before turning 30. (Klich, 2015)
Amoruso ... Show more content on Helpwriting.net ...
By having a more recognizable brand will help Nasty Gal maximize net income, reason being, more
consumers will shop online and spread the word by using social media and face to face. Nasty Gal
should focus on customer–focused behavioural objectives such as increase purchase frequency, by
its current customers and loyal customers. Since Nasty Gal is relatively new to the retail business,
another objective should be to allure new potential customers to make a first– time purchase with
Nasty Gal, resulting in increased purchase frequency which will help increase Nasty Gal's overall
net income. First–time purchases will also help increase the company's net income but will also
increase the number Nasty Gal's loyal customers. Some collaborator objectives should be to extend
distribution coverage, which will definitely increase Nasty Gal's net income. Considering Nasty Gal
just recently released its own Nasty Gal Collection, distributing this exclusive collection to major
department stores will create brand marketing. A few company objectives according to Forbes;
Nasty Gal does barely any brand marketing. (Forbes.com, 2016) In order to increase net income and
to create a more recognized brand, Nasty Gal needs to improve the effectiveness of the company's
marketing actions. As mentioned in collaborator objectives, distribution to major department stores
will create better brand recognition for Nasty Gal. To create more effective marketing, Nasty Gal
should invest in a television commercial to advertise to other potential customers that do not
normally shop online. Potential competitive objectives are that Nasty Gal is currently buying from
new upcoming designers that other major competitors can purchase. Nasty Gal should secure
proprietary access to scarce resources and strengthen its market position. (Forbes.com, 2016) (Anon,
2016) (Pawlowski et al., 2016) (Nastygal.com,

Gal Gadot-Versano Biography
You may hear the name Gal Gadot and instantly think of Wonder Woman. Gal Gadot–Versano is an
amazing woman who is an actress and model. She doesn't have any nicknames and actually goes by
her given birth name. Gal Gadot was born on April 30, 1985 in Petah, Tikva. Her nationality is
considered Israeli since she was born and raised in Israel. Her life as a child was a very educated and
active one. She was surrounded by a Jewish Israeli environment that she still has in her heart. She
attended high school and got a major in biology. She was very good in basketball and got some jobs
in her teenage years. Gal Gadot was a gorgeous woman that she got got crowned Miss Universe in
2004. She also joined Israel Defense Forces and then pursued her acting career. ... Show more
content on Helpwriting.net ...
Her and her husband have two daughters Alma Versano who is five years old and Maya Versano
who is eight months old. Her family makes her really happy and they get to cherish the happy
moments together. Gal Gadot support system before her family was created were her parents. Her
mother, Irit Gadot, is a teacher while her father, Michael Gadot, is an engineer. Both of her parents
were born in Israel as well and provided her with everything she needed. In addition, her grandfather
was one of the survivors of the Holocaust and her grandmother left before the Nazi invasion
occured. She does have an extensive family but all of them are very supportive of her career and are
proud of her. Gal Gadot currently lives in Israel where she has a home but stays in Los Angeles,
California for her acting career. She does live a rich lifestyle due to her accomplishments and gets to
spend quality time with her

P120 Energy And Technology Research Paper
P120: Energy/Energy & Technology Fall 2015 Homework 11 1. The energy that is released in a
fusion reaction comes from the loss of mass. 2. The sun is successful in providing energy via fusion
reactions because its energy output comes from nuclear burning. Nuclear burning is the
transformation of 4 million tons of matter into energy every second. 3. 50m x 2m x 10m= 1000m3
volume 1000m3 water x 1 gal/0.00375m3 x 1 gal deuterium/ 8 gal water = 3.3 x 104 gal deuterium
Need 4000 bbl/day of oil 4000bbl/day x 1 yr/365 days x 5.8x106 Btu/ 1bbl x 1 gal gas/1.24 x 105
Btu = 6.83 x 107 gal gas 5.3 x 109 kWh is enough for I million people for a year, so 5.3 x 108 kWh
would be enough for the 100,000 5.3 x 108 kWh x 1 gal/ 11 kWh= 4.8 x 107

pBlu lab
February 20th, 2014 Lab report 4 Abstract This pBlu lab had for purpose to present the changes of
the strain of E. coli bacteria due to new genetic information being introduced into the cell. In this
experiment we are freezing and heat shocking the E. Coli bacteria that is then forced to take the
plasmid DNA. The E. coli then transforms the pBLu plasmid, which carries the genes coding for
two identifiable phenotypes. After following the Carolina Biological steps our lab worked well and
we able to see some colonies of bacteria on the plates. The x–gal plate showed a significant amount
of bacteria to confirm that the pBlu plasmid took over the E. coli strain. Introduction This lab was
meant to reveal the variations of different ... Show more content on Helpwriting.net ...
LB= Growth medium of Agar/Luria Broth (used in all experiments) AMP= Ampicillin present X–
gal= galactose sugar Clear= no growth at all. The results of this experiment ended up being very
conclusive and not positively or negatively surprising. The data and results collected we what we
had expected more importantly the ones on the X–Gal plates where the colonies were above 100.
Both the stains of E. Coli grew colonies when they were in the LB. When the ampicillin was present
the cells did not grow even with the X–gal. The transformed cells on the other hand grew when the
ampicillin was there. When the X–gal and AMP were present the transformed cells grew and had a
blue tint, which is why we call it the pBlu lab. (duh) Discussion What was expected that the strains
of E. coli that did not have a resistance to ampicillin would not grow. The transformed strain also
changed to a blue color when the X–gal was present in plate. The transformed cells also grew
because they were free of the ampicillin because they possessed the amp gene that they used as an
shield against the ampicillin antibiotic. The transformed cell who turned blue did so because the
gene converted the sugar to a blue color but also contained the amp gene to ensure that they grew
even when the ampicillin was present. The growth of the colonies on the plates

A Safe Haven For Orphans
After the loss of the majority of France's colonies in the Americas, the Restoration government
occupied itself with colonizing attempts in Senegal and French Guiana. During this period of
colonial expansion in French Guiana, the French government endorsed several attempts to colonize
Mana, an area along the fertile, but poorly known Mana River. However, none of their attempts
were successful. In 1827, the French government granted Anne–Marie Javouhey and the Sœurs de
Saint–Joseph de Cluny, the religious congregation that Javouhey founded with four of her biological
sisters, a tract of land for the establishment of an independent commune. Javouhey initially planned
to use Mana to create a safe haven for orphans. Although she asked the French government to
financially support the establishment, the colony was to remain independent in terms of governance
and its economic livelihood. By 1832, before the orphans that she had envisioned could materialize,
Javouhey's attempt had failed. According to Sarah Curtis, of the original fifty–six colonists in 1828,
"five had died, eight had left for other parts of Guiana, and twenty–nine had returned to France." In
1836, Javouhey returned to Mana. This time, she found success working for the moralization and
gradual emancipation of slaves. Commenting on Javouhey's second effort in Mana, Delaplace
stated, "il semblait à beaucoup que c 'était plutôt là l 'œuvre d 'un homme que d 'une femme, si
capable et courageuse fût–elle." His remark

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