G protein-coupled receptor GPR19 regulates the expression of E-cadherin and the invasion of breast cancer cells. The study found that GPR19 promotes the mesenchymal-epithelial transition through the ERK/MAPK pathway, facilitating the colonization of metastatic breast tumor cells. A series of experiments were conducted including western blot, wound healing assay, invasion assay, and quantitative real time PCR to analyze the effects of GPR19 on E-cadherin expression and cancer cell invasion. The results showed that GPR19 plays a potential role in metastasis by promoting the transition from a mesenchymal to epithelial state.

1. G protein-coupled receptor GPR19 regulates E-cadherin
expression and invasion of breast cancer cells
DENNIS N. MUÑOZ, RN, RM, LPT
M.Sci Biology, Ateneo de Davao University
Johannes Hartwig, Katsiaryna Tarbashevich, Jochen Seggewiß, Martin Stehling, Jan Bandemer, Cecilia Grimaldi, Azadeh Paksa, Theresa
Groß-Thebing, Dana Meyen and Erez Raz
Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 31 (August 5, 2014), pp. 11389-11394

2. Introduction

3. E cadherin and GPCR19

4. Material and methods
1. Antibodies and
reagents
2. Cell culture
3. Plasmid constructs and
generation of stable
cell lines
4. Adropin treatment
5. Western blot
6. Wound healing assay
7. Immunocytochemistry
8. Invasion assay
9. TGFα shedding assay
10. Quantitative real time
PCR (QRT-PCR) and
reverse transcriptase
PCR (RT-PCR)
11. Cyclic AMP assay

5. Results

6. Results

7. Conclusion
• This study it shows for the first time that
GPR19 plays a potential role in metastasis by
promoting the mesenchymal-epithelial
transition (MET) through the ERK/MAPK
pathway, thus facilitating colonization of
metastatic breast tumor cells.