LiveSense

Cell -based autonomous biosensing microsystem LiveSense 03/02/12 Nano-Tera.ch Annual Plenary Meeting

Scope of the project ,[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 Nano-Tera.ch Annual Plenary Meeting

The 8 teams in LiveSense project ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Why cell based sensors ? ,[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Mammalian cells : ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting Epithelial cells on chip, CSEM Hepatocytes on-chip, EPFL_LMIS Fibroblats on nanopillars, ETHZ

Genetically modified Bacterial cells: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 Bacteria in beads, Unil

Bacterial biosensors example (Jan van der Meer, UNIL) ,[object Object],03/02/12 nano-tera.ch annual meeting

Emerging contaminants (Jan van der Meer, UNIL) 03/02/12 nano-tera.ch annual meeting Woutersen et al., 2011

Project highlights ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting For detailed information, go to the posters

Bacterial biosensors in microfluidic chips ,[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Bacterial biosensors in microfluidic chips ,[object Object],[object Object],03/02/12 nano-tera.ch annual meeting 0, 10 and 50 µg As/L 60-80 min response time is OK

Electrochemical measurements with bacterial biosensors ,[object Object],[object Object],03/02/12 nano-tera.ch annual meeting LEPA 10 µM As tap

Trans Epithelial Electrical Resistance (TEER) ,[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Trans Epithelial Electrical Resistance (TEER) 03/02/12 nano-tera.ch annual meeting ,[object Object]

Cell contractility toxicology assay 03/02/12 nano-tera.ch annual meeting ,[object Object],[object Object]

Cell contractility toxicology assay 03/02/12 nano-tera.ch annual meeting ,[object Object]

Liver cell bioreactor 03/02/12 nano-tera.ch annual meeting ,[object Object],[object Object],LMIS-4

Assessment of acetaminophen toxicity 03/02/12 nano-tera.ch annual meeting Lab Chip, DOI: 10.1039/C1LC20212J (2011) ,[object Object],[object Object],LMIS-4

Glucose and lactate sensor 03/02/12 nano-tera.ch annual meeting ,[object Object],Glucose Measurement Lactate Measurement SAMLAB

Regulating osmolarity of the sample 03/02/12 nano-tera.ch annual meeting ,[object Object],SAMLAB

System integration: 03/02/12 nano-tera.ch annual meeting ,[object Object],[object Object],50 µg As/L

First system integration: 03/02/12 nano-tera.ch annual meeting Microfluidic cell incubator Fluorescence sensors µ processor + GSM module Power supply Micropumps for perfusion

First demonstration of the concept: Remote fluorescence detection of bacterial cells 03/02/12 nano-tera.ch annual meeting Get SMS with: Reference point Measurement at end point Make florescence measurement Send SMS Send SMS query Start perfusion: Make reference measurement Incubate

Summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

03/02/12 nano-tera.ch annual meeting

Secondary sensors for bacterial sensors ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Secondary sensors for mammalian cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Secondary sensors for cell culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Bacterial biosensors example (Jan van der Meer, UNIL) ,[object Object],[object Object],[object Object],[object Object],[object Object],03/02/12 nano-tera.ch annual meeting

Emerging contaminants (Jan van der Meer, UNIL) ,[object Object],03/02/12 nano-tera.ch annual meeting Woutersen et al., 2011 Green = ‘sufficient’ from perspective of international standards * = only very few specific compounds can be targeted: e.g., BTEX, PAHs, phenols, few herbicides, alkanes Compound class Specific reporters ‘ toxicity’ reporters Heavy metals Low µg/L range mg/L range Organic compounds* µg/L - mg/L range mg/L range Mutagens Not detected µg/L range

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