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2008 Brown iGEM Project Proposal Presented before the Faculty Advising Committee April 9, 2008 Katherine Jacobs Neil Parikh Rima Shah
Guidelines for Ideal Biosensor ,[object Object],[object Object],[object Object],[object Object],[object Object]
Proposed Concept  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Final Construct S R Rz Induced Promoter (IP)
Conductance-Based  Reporting System S R Rz Arabinose-induced promoter S R Rz IP
Arsenic-Induced Promoter S R Rz Arsenic-Induced Promoter  S R Rz IP
Our Goals ,[object Object],[object Object],[object Object],[object Object]
Resistance Measurement ,[object Object],[object Object],[object Object]
Timeline  ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],Timeline
Ideal Biosensor Guidelines Met ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Future applications ,[object Object],[object Object],[object Object],[object Object]
Future applications:  Portable Chemical Sensor 2) Add drop of sample Highly concentrated E.  coli (dried) 1) Add H 2 O to reconstitute dried E. coli 3) Adding sample causes S,R proteins to be expressed if the sample matches 4) Resistance is measured across  5) Circuit board determines if resistance has decreased
Budget OR Needed Cost Source Product Number Chemically Competent E  .coli $271.00 Invitrogen N3011L Shipping DNA from Texas $100 FedEx Getting DNA sequenced $435 @ $0.25 / bp iGEM Sponsor PC Based Multimeter TBD Radiolabs Miscellaneous Apparatus Setup $150 Home Depot Total Expense $950- $1300

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Brown iGEM Toxipop Presentation

  • 1. 2008 Brown iGEM Project Proposal Presented before the Faculty Advising Committee April 9, 2008 Katherine Jacobs Neil Parikh Rima Shah
  • 2.
  • 3.
  • 4. Final Construct S R Rz Induced Promoter (IP)
  • 5. Conductance-Based Reporting System S R Rz Arabinose-induced promoter S R Rz IP
  • 6. Arsenic-Induced Promoter S R Rz Arsenic-Induced Promoter S R Rz IP
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13. Future applications: Portable Chemical Sensor 2) Add drop of sample Highly concentrated E. coli (dried) 1) Add H 2 O to reconstitute dried E. coli 3) Adding sample causes S,R proteins to be expressed if the sample matches 4) Resistance is measured across 5) Circuit board determines if resistance has decreased
  • 14. Budget OR Needed Cost Source Product Number Chemically Competent E .coli $271.00 Invitrogen N3011L Shipping DNA from Texas $100 FedEx Getting DNA sequenced $435 @ $0.25 / bp iGEM Sponsor PC Based Multimeter TBD Radiolabs Miscellaneous Apparatus Setup $150 Home Depot Total Expense $950- $1300

Editor's Notes

  1. We wanted to create a biosensor because biosensor is either expensive or bulky Want one economically feasible and easily transported especially for use in areas such as battlefields or third world countries where toxicity can pose a health concern.