13. Future applications: Portable Chemical Sensor 2) Add drop of sample Highly concentrated E. coli (dried) 1) Add H 2 O to reconstitute dried E. coli 3) Adding sample causes S,R proteins to be expressed if the sample matches 4) Resistance is measured across 5) Circuit board determines if resistance has decreased
14. Budget OR Needed Cost Source Product Number Chemically Competent E .coli $271.00 Invitrogen N3011L Shipping DNA from Texas $100 FedEx Getting DNA sequenced $435 @ $0.25 / bp iGEM Sponsor PC Based Multimeter TBD Radiolabs Miscellaneous Apparatus Setup $150 Home Depot Total Expense $950- $1300
Editor's Notes
We wanted to create a biosensor because biosensor is either expensive or bulky Want one economically feasible and easily transported especially for use in areas such as battlefields or third world countries where toxicity can pose a health concern.