7 10. carlos p glo & sds, nano, biofueled


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7 10. carlos p glo & sds, nano, biofueled

  1. 1. pGlo Bacterial Transformation and SDS Page by Dr. Belinda Roman and Dr. Ricardo Chiesa Bacteria Transformation is a branch of genetics that is commonly used in BiomedicalResearch. By using this technique, we can identify whether proteins are present or are expressingin an organism; proteins like Green Fluorescent Protein (GFP), Lux and others. In order to learnhow this technique works we realized a laboratory about this. This laboratory was divided in twoparts. In the first part we learned how to genetically transform a bacteria in order to expressfluorescence when expose to Ultra Violet light. This was possible by growing bacteria, E. coli, in4 Petri dishes, each with different conditions in the growth medium like the molecularcomponents etc. Then we performed a SDS Page Extension to see the GFP protein in its ultimateexpression. One of the gels (because we realized two gels that contained the same samples) wasused to apply Comassee Blue dye and the other one was to be exposed at UV light. To conclude,it can be establish that we can observe directly the presence of GFP in both of the gels. This waspossible because we did a good pippetting and also a good loading of the samples in the gels. Nanotechnology by Dr. Wilfredo Otaño Nanotechnology is a science which is widely applied in biomedical research. An exampleof the many applications that it can have is the attempts to deposit hidroxioapatite in someinorganic metal or fiber. This seeks to replace human bone. The laboratory of Dr Otañoperformed experiments (for NASA) to find a sensor that is capable of identifying hydrogen in theatmosphere of some other planet. What is done is that a special machine call high vacuum sets anumber of gases and metals in a chip. Metals and gases have to react with the presence ofhydrogen but also when there is not presence of this gas. For this reason, the laboratory realizesmany sensors that can be uset to detect the presence of hydrogen but when the hydrogen is notanymore in the environment the sensors don’t react. This was what happened in our case. Inconclusion surely our technique did not work because the time required was not enough toperform a sensor of quality. Also it was not made with the appropriate details and precautionsthat have to be taken when performing such experiments. Biofuel: Enzyme Kinetics by Dr. Ricardo Chiesa Enzymes are proteins that act as catalysts in biological and metabolic processes thatoccur in organisms. An interesting fact is that the enzyme activity can be deregulated ordenaturalized by factors such as pH and temperature. During this laboratory we realized severalprocedures that put enzymes under different stress factors in order to see the different reactionsof the enzymes. In our case we exposed enzymes at different temperatures to see their productioncapacity in both of enzymatic status: denatured and native state. Valeria and I put the enzyme atdifferent temperatures. The results showed that the enzyme that was exposed to room
  2. 2. temperature maintained the enzymatic integrity and thus had a greater capacity for work. On theother hand, the enzymes that were exposed to hot or cold temperatures had a low enzymaticcapacity respectively. This shows that the temperature factors do affect the integrity of theenzyme or denaturation process. Carlos Santos Perez 804-10-8280 University of Puerto Rico Cayey P.R.