Need Bacteria Factor VIII Bio wiz please, last chance!! Incorrect. At least one of the statements you have selected is incorrect. Think about the similarities and differences between transcription and translation in both eukaryotes and prokaryotes. What cellular machinery involved is similar in each type of organism? Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor ViI. Not all steps will be placed. Continued below... solate the human gene that produces factor VIlI. The factor VIlI protein is isolated from human tissues. Isolate the mRNA of the factor VIlIl gene. Generate cDNA of the factor VIlI gene using reverse transcriptase.gene Insert the factor VIlII cDNA into a bacterial vector near a promoter site Transform the vector into an E. coli bacterium. Human DNA is introduced into the bacterial cell using a projectile gun and DNA-coated tungsten E. coli expresses factor VIII. Scroll up for feedback Solution In the first section the last statement you have selected is incorrect i.e. there is no fit for the sentence \"Human DNA is introduced into the bacterial cell using a projectile gun and DNA coated tungsten\" as in the process of transformation the plasmid vector containing factor VII gene under a bacterial promoter is already introduced inside the bacterial cell. The plasmid vector containing factor VII gene under a bacterial promoter inside the bacterial cell will be under bacterial promoter control. Universality of DNA sequence will allow it to transcribe but codon biasness may hinder the expression. There is degeneracy of genetic codon i.e. alternative triplet codon is used to mean for amino acids. When the triplet code for no amino acid (stop codon) or tRNA for that code is not available the transcription terminates. If the tRNA is not readily available then the transcription eventually terminates. So E. coli is added with another plasmid like RIL/RIPL plus to supplement those tRNA those are frequently and exclusively used in eukaryotic sequence. In RIPL plus cess there is no limited tRNA for R, I, P and L so the code reads without attenuation. So the Answer to the second question is \"The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the factor VII gene. Instead of supplementing RIPL it would have been managed by modifying codon of the introduced gene of factor VII for bacteria optimised codons but in the question it is mentioned that the gene is unmodified. The eukaryotic gene under bacterial promoter can be transcribed and translated using prokaryotic system. The intronic sequence is not present in the cDNA when it is reverse transcribed from mRNA as introns are processed or removed in processed mRNA. The plasmid vector containing factor VII gene under a bacterial promoter inside the bacterial cell..

1. Need Bacteria Factor VIII Bio wiz please, last chance!! Incorrect. At least one of the statements
you have selected is incorrect. Think about the similarities and differences between transcription
and translation in both eukaryotes and prokaryotes. What cellular machinery involved is similar
in each type of organism? Order the steps that would be used in a laboratory to engineer a
bacterium that could express the human gene coding for factor ViI. Not all steps will be placed.
Continued below... solate the human gene that produces factor VIlI. The factor VIlI protein is
isolated from human tissues. Isolate the mRNA of the factor VIlIl gene. Generate cDNA of the
factor VIlI gene using reverse transcriptase.gene Insert the factor VIlII cDNA into a bacterial
vector near a promoter site Transform the vector into an E. coli bacterium. Human DNA is
introduced into the bacterial cell using a projectile gun and DNA-coated tungsten E. coli
expresses factor VIII. Scroll up for feedback
Solution
In the first section the last statement you have selected is incorrect i.e. there is no fit for the
sentence "Human DNA is introduced into the bacterial cell using a projectile gun and DNA
coated tungsten" as in the process of transformation the plasmid vector containing factor VII
gene under a bacterial promoter is already introduced inside the bacterial cell.
The plasmid vector containing factor VII gene under a bacterial promoter inside the bacterial cell
will be under bacterial promoter control. Universality of DNA sequence will allow it to
transcribe but codon biasness may hinder the expression. There is degeneracy of genetic codon
i.e. alternative triplet codon is used to mean for amino acids. When the triplet code for no amino
acid (stop codon) or tRNA for that code is not available the transcription terminates. If the tRNA
is not readily available then the transcription eventually terminates. So E. coli is added with
another plasmid like RIL/RIPL plus to supplement those tRNA those are frequently and
exclusively used in eukaryotic sequence. In RIPL plus cess there is no limited tRNA for R, I, P
and L so the code reads without attenuation.
So the Answer to the second question is "The gene will induce attenuation in the bacterial cell,
which will cause a delay in the transcription and translation of the factor VII gene.
Instead of supplementing RIPL it would have been managed by modifying codon of the
introduced gene of factor VII for bacteria optimised codons but in the question it is mentioned
that the gene is unmodified.
The eukaryotic gene under bacterial promoter can be transcribed and translated using prokaryotic
system.
The intronic sequence is not present in the cDNA when it is reverse transcribed from mRNA as

2. introns are processed or removed in processed mRNA.
The plasmid vector containing factor VII gene under a bacterial promoter inside the bacterial
cell.