5.3 - Group Project: Business Review Presentation (Just do section 3). 10 ppt slides with speaker notes -Product/Country: Power Generators/U.S. to Liberia In this assignment, you will be working in the groups assigned in Module 4 to develop a Microsoft PowerPoint® presentation. The group presentation will include the methods and requirements for a global business plan consisting of the following elements: 1.The selection of an International Business (IB) product/service to be marketed and sold in a foreign country.(do not do) 2.The management activities required to analyze the Internal Environment and the expected results from country and product/service selection.(do not do) ***3.The economic, political, legal, religious, cultural, and business activities surrounding the External Environment for the countries and product/service under consideration. ( This is the section I will be doing 10 slides with speaker notes)**** REMEMBER: Your assignment can only be submitted once. •Current APA Format •Cite All Sources •Speaker notes for every slide ◦How to Add Speaker Notes to Your Slides (Links to an external site.)Links to an external site. •Presentation should average 15 - 20 minutes in length (Judge time as if presented to a live audience) Pseudomonas has a plasmid containing the mer operon, which includes a gene for mercuric reductase. The enzyme catalyzes the reduction of the mercuric ion Hg2+ to the uncharged form of mercury, Hg0  Hg2+ is quite toxic to cells, Hg0 is not.  1. What condition would induce this operon to be transcribed and the transcript to be translated into the proteins including mercuric reductase?  2. The protein encoded by the mer operon binds to Hg2+ outside the cell and brings it into to the cell, why would a cell bring in a toxin?  Two daughter cells are most likely to inherit which one of the following from a parent cell?  3. a change in a nucleotide in mRNA, tRNA or rRNA?  4. Which of the following is NOT a method of horizontal gene transfer? Contrast two methods of horizontal gene transfer.  conjugation,  integration of a transposon, transduction or transformtion 5. Amino acid sequences for the viral coat of HIV were sequenced from three HIV patients.  Of the amino acid sequences shown which are most closely related?  Patient   A     Asn Gln Thr Ala Ala Ser Lys Asn Ile Asp Ala Leu Patient B      Asn Leu His Ser Asp Lys Ile Asn Ile Ile Leu Leu Patient C       Asn Gln Thr Ala Asp Ser Ile Val Ile Asp Ala Leu 6. The following is a code for a strand of DNA.                  1 2 3 4 5  6 7    8 9 10 11 12 13 14 15 16 17 18 19 DNA    3' A T A T  - - -  T T T  _ _ _ _ _ _ _ _                                                       11 12 13         17 18 19 mRNA                                            C G  U             U  G   A  _ tRNA                                                          U G  G                        6 78 Amino Acid   Met ____________ ___________ ______________ ____________  First fill in the blank.

1. 5.3 - Group Project: Business Review Presentation
(Just do section 3). 10 ppt slides with speaker notes
-Product/Country: Power Generators/U.S. to Liberia
In this assignment, you will be working in the groups assigned
in Module 4 to develop a Microsoft PowerPoint® presentation.
The group presentation will include the methods and
requirements for a global business plan consisting of the
following elements:
1.The selection of an International Business (IB)
product/service to be marketed and sold in a foreign country.(do
not do)
2.The management activities required to analyze the Internal
Environment and the expected results from country and
product/service selection.(do not do)
***3.The economic, political, legal, religious, cultural, and
business activities surrounding the External Environment for the
countries and product/service under consideration. ( This is the
section I will be doing 10 slides with speaker notes)****
REMEMBER: Your assignment can only be submitted once.
•Current APA Format
•Cite All Sources
•Speaker notes for every slide ◦How to Add Speaker Notes to
Your Slides (Links to an external site.)Links to an external site.
•Presentation should average 15 - 20 minutes in length (Judge
time as if presented to a live audience)
Pseudomonas has a plasmid containing the mer operon, which

2. includes a gene for mercuric reductase. The enzyme catalyzes
the reduction of the mercuric ion Hg2+ to the uncharged form of
mercury, Hg0 Hg2+ is quite toxic to cells, Hg0 is not.
1. What condition would induce this operon to be transcribed
and the transcript to be translated into the proteins including
mercuric reductase?
2. The protein encoded by the mer operon binds to Hg2+ outside
the cell and brings it into to the cell, why would a cell bring in
a toxin?
Two daughter cells are most likely to inherit which one of the
following from a parent cell?
3. a change in a nucleotide in mRNA, tRNA or rRNA?
4. Which of the following is NOT a method of horizontal gene
transfer? Contrast two methods of horizontal gene transfer.
conjugation, integration of a transposon, transduction or
transformtion
5. Amino acid sequences for the viral coat of HIV were
sequenced from three HIV patients. Of the amino acid
sequences shown which are most closely related?
Patient A Asn Gln Thr Ala Ala Ser Lys Asn Ile Asp Ala Leu
Patient B Asn Leu His Ser Asp Lys Ile Asn Ile Ile Leu Leu
Patient C Asn Gln Thr Ala Asp Ser Ile Val Ile Asp Ala Leu
6. The following is a code for a strand of DNA.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
DNA 3' A T A T - - - T T T _ _ _ _ _ _ _ _
11 12 13 17 18 19
mRNA C G U U G A _
tRNA U G G
6 78
Amino Acid Met ____________ ___________
______________ ____________
First fill in the blanks to complete the DNA strand, Use the
position numbers to align with mRNA, RNA and the protein.
7. What would be the effect C were substituted for T at base
(DNA) position 10?
What would be the effect if A were substituted for G at base

3. (DNA) position 11?
What would be the effect if C were inserted between bases 9
and 10?
8.
Lable a-l. Remember, you will be drawing and lableing the
DNA replication fork on Lecture Exam III.
9. What is the following region of DNA called and how does it
work? Explain the differences between an inducible and a
repressible version.
10. Fill in following concept map on types of mutations:
11. Table 8.1 on page 240 gives examples of restriction
enzymes. Restriction enzymes cut double stranded DNA at
specific sites, or palindromes. Restriction enzymes serve to cut
up phage DNA so that bacterial chromosomes are not mixed
with phage genes. Restriction enzymes do not cut bacterial
DNA because of methylation. There are two groups of
restriction enzymes based on the way the two strands of DNA
are cut. One type leaves blunt ends and the other sticky ends.
Restriction enzymes may be used to make a cut in a plasmid, a
vector, and insert a gene and use ligase to connect the gene to
the cut ends of the plasmid.
Give an example of one restristion enzyme that makes sticky
ends and one that makes blunt ends. Explain the origin of the
enzymes' names.
12. For decades researchers at Duke Medical School have
worked to engineer a polio virus to treat glioblastoma. The
strategy is to remove the genes of the virus that cause disease

4. polio and replace them with genes for a cold virus, leave the
genes that allow the virus to enter the glial cell, and the genes
that allow the virus to lyse or destroy the cell it enters for
replication. LIst and explain two 'tools of recombinant DNA
technology' explained in chapter 8 that could be used to create
this modified polio virus. Table 8.2 is a summary of t he tools
of DNA technology. p 250.
13. A large long term study on the genomic effects of PTSD
utlizes DNA microarrays to screen for changes in gene
expression of subjects in the study. Some of the preliminary
data has identified clusters of genes that change with stress.
What about this technique allows for screening of hundreds of
genes on a single glass slide? If the microarray uses cDNA,
how does cDNA differ from genomic DNA? Why must the
DNA on the slide be ssDNA?
14. Explain the function and use of reverse transciptase in
synthesizing cDNA? How does HIV use reverse transcriptase?
What drug in a cocktail (ART) used to treat AIDS targets
reverse transcriptase? p.543
15.
Please label this technique. What is the technique? Explain at
least two applications.
16. PCR utilizes taq polymerase, what is the adavantage of this
type of DNA polymerase? Why can't DNA polymerase from E.
coli be used?
17. Why are mutagens tools of recombinant DNA technology?
Give two examples of mutagens and their mode of action.
There is a market for salmon, however salmon takes three years
to mature which increases the cost of salmon. AquaBounty first
presented a fast growing fish in 1989 by combining a growth
hormone gene from the Chinook salmon and a reglatory gene
from the ocean pout to create a continouos low level of growth
hormone in the Atlantic salmon. The genetically modified
salmon grows in 18 months, may be grown in fish tanks near

5. cities thus reducing the need for air transport and tank grown
salmon have fever parasites. AquaBounty applied for FDA
approval over 25 years ago, and in 2017 meet another hurdle to
selling in the US because the FDA requires a GMO labeling, for
which a program for which is under development. Canadian
customers have bought 4.5 tons of this fish as of August 4,
2017.
18. Speculate about why genetically modified foods should be
labled for the consumer?
19. Give at least two examples of recombinant DNA techniques
that might be used to introduce genes into fish.
20. Fill in the boxes on the concept map below:
Biotechnology is the use of microbes to make practical
products. The top three justifications for manipulating the
genomes of cells are:
1. to eliminate undesirable phenotypic traits in humans,
animals, plants and microbes.
2. to combine the beneficial traits of two or more organisms to
create a new more valuable organism.
3. to create cells that synthesize products humans need.
Recombinant DNA technology employs a number to tools and
techniques to isolate genes and insert them into cells grown in
culture. The following are five tools of recombinant DNA
technology and examples of application of the tools.
Mutagens are physical and chemical agents that produce
mutation. Some mutations are beneficial, so creating a large
number of mutations increases the probability of discovering a
beneficial mutation. For example the fungus Penicillium may be
mutated to syntheisize a more effective antibiotic. Mutagens
include uv radiation and ionizing radiation. Mueller first
demonstrated the effects of X rays on Drosophilia,establishing
cause and effect between radiation and genetic changes
manifested in phenotypic changes. Chemical mutagens in

6. bacteria may be carcinogenic in humans. The Ames test utilizes
bacteria to test for mutagenic activity, and identify chemicals
that may induce cell changes that lead to cancer. Ethidium
bromide which may be used to visualize DNA on gel, is also a
mutagen because it disrupts base pairing.
1. Explain and describe one physical and one chemical mutagen
and its application.
Reverse transcriptase synthesizes cDNA from mRNA, which is
the reverse of information flow as depicted in the central dogma
of molecular biology, i.e. DNA is transcribed to RNA which is
translated to peptides. Cells may have millions of copies of
mRNA, so being able to synthesize the complementary DNA
reveals the gene that is being expressed. In addition, cDNA
contains no introns because of processing in the eukaryotic
transcription, so that cDNA may be inserted into a prokaryotic
cell and may be translated into the corresponding peptide.
2. Explain how reverse transcriptase differs from RNA
polymerase. Give one example of an application of reverse
transcriptase in recombinant DNA technology.
Synthetic nucleic acids are synthesized in vitro using enzymes
from cells. Currently there are machines that syntheized DNA or
RNA by having the sequence of bases entered tthrough a
keyborad.
3. If each letter of the keyboard represents a base in DNA, how
many keys does the keyboard require? How does this means of
DNA synthesis differ from DNA replication in the cell?
Synthetic nucleic acids were used to elucidate the genetic code,
create genes for specific proteins, synthesize probes to locate
genes in a genome, to synthesize antisense nucleic acids and to
make PCR primers.
4. What is the genetic code, how might a synthetic nucleotide be
used to determine which amino acid corrresponds to which
codon?
5. Give an example of a probe with a flourescent tag that will be
used to locate a gene associated with an aggressive form of
breast cancer.

7. Restiction enzymes cut dsDNA at restriction sites, or
palindromes. Examples of restriction enzymes are EcoRI and
HindIII. There are hundreds of restriction enzymes isolated
from bacteria and used to cut DNA at predictable sites. One
type of restriction enzyme cuts dsDNA to make blunt ends and
the other type creates sticky ends.
6. Compare and contrast blunt and sticky ends, and give one
example of each type of restriction enzyme.
Vectors insert DNA into a new cell so that the cell acquires a
new phenotype. Vectors are pieces of DNA that are small
enough to manipulate in a lab, survive inside the new cell,
contain a recognizalbe genetic marker and carry the gene
regions necessary to ensure the gene is transcribed and
translated. Examples of vectors are plasmids, viruses and
transposons.
7. Explain one risk of a viral vector that inserts itself into a
necessary gene and causes a mutation.
Gene library is a collection of cells or viruses, in which each
member carries a portion of a given organism's genome. Gene
libraries provide a ready
Techniques of recombinant DNA technology include the
polymerase chaing reaction (PCR) developed by Cary Mullis.
PCR has three steps that are repeated while the same sample of
primers, target gene, taq polymerase and nucleotides are cycled
through three different temperatures in a microfuge tube. Gel
electrophoresis separated fragments of molecules by size, shape
and charge. DNA microarrays are able to monitor thousands of
genes on one plate. Applications of these techniques include
diagnostics or vaccine design.
8. Explain how a subunit vaccine is designed using genes from
a pathogen and a viral vector? p502-504.
One proposed way to stop the spread of arboviruses by
mosquitoes is to vaccinate the mosquito. This type of vaccine
is a transmission blocking vaccine (TBV). One typeof TBV
vaccinates the human so that a blood meal from a vaccinated
human would prevent the mosquito from being infected.

8. Another strategy is to use the gene drive known as Crispr-Cas9
which would edit the mosquito genome to confer resistance to
diseases mosquitoes transmit to humans, and is very targeted.
This requires wild type mosquitoes to mate with lab mosquitoes
that have been genetically modified. Some data would indicate
the lab mosquitoes are not competitve with wild type males.
9. Explain one advantage and one disadvantage of the TBV
approach for controlling the spread of arboviruses.
10. Does the public have any control over the use of gene drives
to alter species in the ecosystem for public health concerns?