5.3 - Group Project: Business Review Presentation
(Just do section 3). 10 ppt slides with speaker notes
-Product/Country: Power Generators/U.S. to Liberia
In this assignment, you will be working in the groups assigned in Module 4 to develop a Microsoft PowerPoint® presentation.
The group presentation will include the methods and requirements for a global business plan consisting of the following elements:
1.The selection of an International Business (IB) product/service to be marketed and sold in a foreign country.(do not do)
2.The management activities required to analyze the Internal Environment and the expected results from country and product/service selection.(do not do)
***3.The economic, political, legal, religious, cultural, and business activities surrounding the External Environment for the countries and product/service under consideration. ( This is the section I will be doing 10 slides with speaker notes)****
REMEMBER: Your assignment can only be submitted once.
•Current APA Format
•Cite All Sources
•Speaker notes for every slide ◦How to Add Speaker Notes to Your Slides (Links to an external site.)Links to an external site.
•Presentation should average 15 - 20 minutes in length (Judge time as if presented to a live audience)
Pseudomonas has a plasmid containing the mer operon, which includes a gene for mercuric reductase. The enzyme catalyzes the reduction of the mercuric ion Hg2+ to the uncharged form of mercury, Hg0 Hg2+ is quite toxic to cells, Hg0 is not.
1. What condition would induce this operon to be transcribed and the transcript to be translated into the proteins including mercuric reductase?
2. The protein encoded by the mer operon binds to Hg2+ outside the cell and brings it into to the cell, why would a cell bring in a toxin?
Two daughter cells are most likely to inherit which one of the following from a parent cell?
3. a change in a nucleotide in mRNA, tRNA or rRNA?
4. Which of the following is NOT a method of horizontal gene transfer? Contrast two methods of horizontal gene transfer.
conjugation, integration of a transposon, transduction or transformtion
5. Amino acid sequences for the viral coat of HIV were sequenced from three HIV patients. Of the amino acid sequences shown which are most closely related?
Patient A Asn Gln Thr Ala Ala Ser Lys Asn Ile Asp Ala Leu
Patient B Asn Leu His Ser Asp Lys Ile Asn Ile Ile Leu Leu
Patient C Asn Gln Thr Ala Asp Ser Ile Val Ile Asp Ala Leu
6. The following is a code for a strand of DNA.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
DNA 3' A T A T - - - T T T _ _ _ _ _ _ _ _
11 12 13 17 18 19
mRNA C G U U G A _
tRNA U G G
6 78
Amino Acid Met ____________ ___________ ______________ ____________
First fill in the blank.
Describe the application of DNA profiling in paternity tests and forensic investigations
Analyze DNA profiles to draw conclusions about paternity tests and forensic investigations.
Describe the application of DNA profiling in paternity tests and forensic investigations
Analyze DNA profiles to draw conclusions about paternity tests and forensic investigations.
Assignment on Recombinant DNA Technology and Gene TherapyDeepak Kumar
Assignment on Recombinant DNA Technology and Gene Therapy Basic principles of recombinant DNA technology-Restriction enzymes, various types of vectors, Applications of recombinant DNA technology. Gene therapy- Various types of gene transfer techniques, clinical applications and recent advances in gene therapy
This is one of the major chapters for the examination NEET. A few questions are expected from this chapter and carry more weight as per the NEET syllabus.
M Pharm Pharmacognosy Semester 2, MEDICINAL PLANT BIOTECHNOLOGY UNIT 1, Introduction to Plant biotechnology: Historical perspectives, prospects for development of plant biotechnology as a source of
medicinal agents. Applications in pharmacy and allied fields. Genetic and molecular biology as applied to pharmacognosy, study of DNA, RNA and protein replication, genetic code, regulation of gene expression, structure and complicity of
genome, cell signaling, DNA recombinant technology.
· Present a discussion of what team is. What type(s) of team do .docxalinainglis
· Present a discussion of what team is. What type(s) of team do you have in your organization?
· What is meant by the “internal processes” of a team? Why is it important to manage both the internal processes and external opportunities/constraints of a team?
Note: It should contain 3 pages with citation included and References should be in APA format
.
· Presentation of your project. Prepare a PowerPoint with 8 slid.docxalinainglis
· Presentation of your project. Prepare a PowerPoint with 8 slides illustrating the role in Interdisciplinary care for our aging population (Outcome 1,2,3,4,5) (6 hours).
Make sure it has nursing diagnosis
make sure it's a APA STYLE
make sure it has reference
.
More Related Content
Similar to 5.3 - Group Project Business Review Presentation(Just do .docx
Assignment on Recombinant DNA Technology and Gene TherapyDeepak Kumar
Assignment on Recombinant DNA Technology and Gene Therapy Basic principles of recombinant DNA technology-Restriction enzymes, various types of vectors, Applications of recombinant DNA technology. Gene therapy- Various types of gene transfer techniques, clinical applications and recent advances in gene therapy
This is one of the major chapters for the examination NEET. A few questions are expected from this chapter and carry more weight as per the NEET syllabus.
M Pharm Pharmacognosy Semester 2, MEDICINAL PLANT BIOTECHNOLOGY UNIT 1, Introduction to Plant biotechnology: Historical perspectives, prospects for development of plant biotechnology as a source of
medicinal agents. Applications in pharmacy and allied fields. Genetic and molecular biology as applied to pharmacognosy, study of DNA, RNA and protein replication, genetic code, regulation of gene expression, structure and complicity of
genome, cell signaling, DNA recombinant technology.
· Present a discussion of what team is. What type(s) of team do .docxalinainglis
· Present a discussion of what team is. What type(s) of team do you have in your organization?
· What is meant by the “internal processes” of a team? Why is it important to manage both the internal processes and external opportunities/constraints of a team?
Note: It should contain 3 pages with citation included and References should be in APA format
.
· Presentation of your project. Prepare a PowerPoint with 8 slid.docxalinainglis
· Presentation of your project. Prepare a PowerPoint with 8 slides illustrating the role in Interdisciplinary care for our aging population (Outcome 1,2,3,4,5) (6 hours).
Make sure it has nursing diagnosis
make sure it's a APA STYLE
make sure it has reference
.
· Prepare a research proposal, mentioning a specific researchabl.docxalinainglis
· Prepare a research proposal, mentioning a specific researchable title, background, Review of literature, research questions and objectives, methodology, resources and references.
· Prepare the Gant Chart to indicate the timescale for completing the proposal
RESEARCH PROPOSAL OUTLINE
1. Title
2. Background (introduction)
3. Review of literature
4. Research Questions & objectives
5. Methodology
4.1 Research Design
4.2 Participants
4.3 Techniques
4.4 Ethical Considerations
6. Time scale (Gantt chart)
7. Resources
8. References
.
· Previous professional experiences that have had a profound.docxalinainglis
· Previous professional experiences that have had a profound effect:
Before I started college, my parents wanted me to excel in healthcare knowing its high demand. The path to health care and eventual employment in a notable hospital setting seemed less risky than the one of Art and design. A few networking events and some LinkedIn leads later I came across an opportunity to start a Biomedical Engineering startup in South Florida with two investors willing to mentor me in a field I wasn’t familiar with. Luckily this new venture I was undertaking had a somewhat speculative risk. I made sure they were mostly in my favor thanks to the connections my investors had in the industry, and my background in health care. My hard work and diligence paid off slowly teaching myself the mechanics of the industry through the engineers we would hire. I remember watching how they would calibrate medical devices from pumps to life-saving equipment in awe. And with the same tenacity absorbing all the medical jargon in the Biomed world. I was adamant about doing my best and being the best even if that meant leaving my creative dreams behind. We started the business almost four years ago as a small minority women-owned business in the corner of a business complex. Five biomedical engineers and six technicians later we are still scaling and have since expanded our office from that small corner to the entire business building. Currently, we are a nationally recognized Biomed and medical supply company for some of the largest healthcare facilities in both the civilian and government sector. Yet through out all the achievement I felt the only sense of raw passion was when I collaborated with my engineers in delivering problem solving services to the hospital we served. Their job was to service devices in a hospital at a micro level and I would bridge that gap by identifying problems and finding opportunities in product service at a large-scale. Working hand in hand with the engineers in articulating the hospital need for turnover I would use design through projective process in creating a plan that would work in the most practical sense.
This moment of free creative problem solving was the highlight of my job. It gave me an opportunity to realize that although at times my approach was unconventional it would work. My systematic methodology I had adapted from working with engineers and my innate out of the box idea would come to together to solve some of the most challenging issues. Little did I know that this minor stroke of self-awareness would one day have me consider architecture.
Your current strengths and weaknesses in reaching your goal.
I realized my creative talents in design could not flourish under the pressures of work. I would constantly leave the office feeling drained in a profession my heart was not set on. In this I learned my weakness was how far I was willing to neglect the urge for creativity, and in exchange it jeopardized my sense of purpos.
· Please select ONE of the following questions and write a 200-wor.docxalinainglis
· Please select ONE of the following questions and write a 200-word discussion.
1. The Federal Reserve Board has enormous power over people's lives with its power to set and influence policy that determines monetary policy in the United States. Do you think this is proper for a democracy to provide the FED with so such power? How is the FED held accountable?
2. Do you believe that the roles of government should change from era to era, or should the US determine the proper role of government and try to maintain it through the ages?
3. Explain Executive Power in the US Constitution and briefly the process by which it developed over the years. Do you think the Framers should have been more specific about the powers of the presidency? Should the country try to make it more specific today?
· Please read the discussions below and write a 100 to 150 words respond for each discussion.
1. (question 1) I do believe that this is proper for a democracy to provided such power to FED. Without the FED the economy would face two problem, which are recessions that can lead into depressions, and inflation. The FED needs to have power to endures the country will not fall into economic trouble. In class professor McWeeney stated that the FED has the power to increase interest rates to control inflation, and the power to decrease interest rates so that theres more money in the economy to create more business and jobs so there wont be a recession. The FED needs these power to try to put the economy in a sweet spot. The FED is held accountable to the government and public. The FED does this by being transparent and giving and annual report to congress.
2. (question 2) I believe that the roles of the government should be changed from era to era. My main reason the roles should be changed is because major changes are constantly happening in the field of law. For example, the progressive era and modern era had several economic reforms that had taken place including increased regulation, anti-trust activity, application of an income tax, raise on social insurance programs, etc. Throughout this time, the government gave women the right to vote. I believe the economy is growing rapidly due to employment relationships, better technology, education, new polices, social and economic changes. This is the reason why the roles of the government should be changed from era to era.
Communicating professionally and ethically is one of the
essential skill sets we can teach you at Strayer. The following
guidelines will ensure:
· Your writing is professional
· You avoid plagiarizing others, which is essential to writing ethically
· You give credit to others in your work
Visit Strayer’s Academic Integrity Center for more information.
Winter 2019
https://pslogin.strayer.edu/?dest=academic-support/academic-integrity-center
Strayer University Writing Standards 2
� Include page numbers.
� Use 1-inch margins.
� Use Arial, Courier, Times New Roman.
· Please use Firefox for access to cronometer.com16 ye.docxalinainglis
· Please use
Firefox
for access to
cronometer.com
16 years old Female. Born on 01/05/2005. Height 5’4, 115 lbs
· Menu Analysis
DAY 2
Quesadilla
Fiesta beans
Salsa
Sour cream
Corn
Fruit
· Submit Screen Shot for Nutrient report for assignment menu(s)
§ Right click to use “Take a screenshot” feature (Firefox only) on specific date you want to have screen shot to save/obtain.
Nutrient Report and Food Intake
· The paper must include all required elements including
each
Cronometer, Excess, Deficit, and
G
roup
Summary of your nutrient report and food intake
Excess
:
· List
ALL
Nutrients that are
Over 100% (Except Amino Acids)
on Cronometer Nutrient report
· List
Food Items
on menu that may reflect excess nutrients on Cronometer Nutrient report
Deficit
:
· List
ALL
Nutrients that are
Less than 50% (Except Amino Acids)
on Cronometer Nutrient report
· List
Food Items
on menu that may reflect deficit nutrients on Cronometer Nutrient report
Summary
:
§ Summarize your overall in 1-2 paragraph, evaluation and conclusion of nutrients and food items on the menu.
.
· Please share theoretical explanations based on social, cultural an.docxalinainglis
· Please share theoretical explanations based on social, cultural and environmental factors, which may contribute to victimization from criminal behavior
· Based on your personal or professional experience share your thoughts on what coping mechanism (internal and external), and support processes can be considered if becoming a crime victim?
.
· If we accept the fact that we may need to focus more on teaching.docxalinainglis
· If we accept the fact that we may need to focus more on teaching civic responsibility, how can this work with both "policies and people" in the school where you become principal?
In order to increase the focus on teaching civic responsibility, policy must be in place supporting this goal. A school leader must be willing to invest time and funds into planning, training, and implementing curriculum that emphasizes civics. Staff members may have different levels of interest, understanding, and comfort when it comes to incorporating civic responsibility into their teaching, so providing professional development in this area would be critical. The strategic plan for integrating civic responsibility and the expectations for each teacher’s involvement should be clearly communicated. In addition to establishing these policies regarding civics education, the school leader and teachers must work to model civic responsibility. In addition to sharing his or her vision for increased focus on civics with the school staff, the school leader should work to share his or her vision with school board members, other district personnel including the superintendent, and the greater community. Lastly, school leaders need to support their staff as they take risks and work to develop and implement new activities, discussions, and projects centered around teaching civic responsibility.
· How will you lead your staff in this part of the curriculum?
In leading my staff in this part of the curriculum, I would work to secure professional development related to civic responsibility, as this is not an area that I have expertise in, and work as a staff to develop our vision and implementation goals. I would also provide examples such as the work of the exemplar schools described in the article in integrating civic responsibility across all content areas, implementing service-learning programs, and creating partnerships between the school and community. I would also work within PLTs to develop ways that civic responsibility could be incorporated within their curriculum and remind them that they have my support as they embark on this endea
Required Resources
Text
Baack, D. (2017). Organizational behavior (2nd ed.). Retrieved from https://ashford.content.edu
· Chapter 8: Leadership
Articles
Austen, B. (2012, July 23). The story of Steve Jobs: An inspiration or a cautionary tale? (Links to an external site.)Links to an external site.Wired. Retrieved fom http://www.wired.com/2012/07/ff_stevejobs/all/
Charan, R. (2006). Home Depot’s blueprint for culture change. Harvard Business Review. 84(4), 60-70. Retrieved from EBSCOhost database
Grow, B., Foust, D., Thornton, E., Farzad, R., McGregor, J., & Zegal, S. (2007). Out at home depot (Links to an external site.)Links to an external site.. Business Week.
Retrieved from http://www.businessweek.com/stories/2007-01-14/out-at-home-depot
Stark, A. (1993). What's the matter with business ethics? Harvard Business Review, 71(3), 38-48. .
· How many employees are working for youtotal of 5 employees .docxalinainglis
· How many employees are working for you?
total of 5 employees
· How did you get your idea or concept for the business?
· CLEAR is a reflection by transparency, manifest and understood, our product is new in the market, and it follows the international fashion style that suits every lady,
· A bag represents you, bags are women priority, and its something women can't go outside without, our bags differ by other bags is that its clear, which is the new form of fashion style, we also made customization on bags so it is a remarkable tool that can lead to higher profit through increased customer satisfaction and loyalty, although it brings for our small factory a lot of work, the good work pays off, we entered these industry because there are no locals designer in it and we started in2016 and hope to reach a global position.
· What do you look for in an employee? (the most important things)
- helping customers on their choice
-stylist
- team work spirit
- deciplant & committed to work ethics
- Good Communication skills
- Ability to manage the conflict
- Is the company socially responsible?
Yes , we try our best to make some of sell go for the charity and especially to help poor people get new clothes , we donate 5% yearly in our total sales .
· What made you choose your current location?
Main criterias for selecting current location :
1- Close to the residence areas , meliha road, near the university of Sharjah
2- Easy access to the visiting customers
3- Its in a big avenue that has many designers and clothing brands
4- Easy to pick up from the shop
5- Serve a big segmentation
· What are your responsibilities as a business owner?
the main responsibility of the Business owner is to maintain the successful of the business, but in order to achieve this have to do so many tasks like:
1- Hire and manage the staff
2- Oversees the financial status , weekly and monthly .
3- Create marketing plans of how the business will be in a year
4- Update the website and chick the system
5- Rent fees
6- Make sure how customers are satisfied by the product
7- Make sure about product quality and chick up
8- Maintain a healthy work environment
9- Develop and fine tune the business according to the market situation
· How do you motivate your employees?
We follow different methods for motivations
1- Personal appreciation for individuals for hard work or personal achievements
2- Kind words
3- Flexible working hours
4- Daily bonus if achieved the daily sales targets
5- Giving the new collection bags as a gift before dropping it to the market , it makes them feel appreciated and special
· Can you give me an example of any challenges or problems that you faced with your shop and employees?
Hiring the right employee is always challenge, last Ramadan we had a huge unread massage for eid orders as well, our customer started to get angry and write under the inestgram comments that there was no respond for online shopping , we struggl.
· How should the risks be prioritized· Who should do the priori.docxalinainglis
· How should the risks be prioritized?
· Who should do the prioritization of the project risks?
· How should project risks be monitored and controlled?
· Who should develop risk responses and contingency plans?
· Who should own these responses and plans?
Introduction
This week, we will explore risk management. Risk management is one of those areas in project management that separates good project managers from great project managers. A good project manager makes risk management an integral part of every phase of project work. Risks are identified, prioritized, and understood. There are clear responsibilities within the team as to whose is responsible for implementing a risk response to reduce the impact should it occur. So let's get started.
What is Risk?
*Risk: An uncertain event or condition that, if it occurs, has a positive or negative effect on one or more project objectives.
Risks can be positive, meaning beneficial to the project, or they can be negative, meaning detrimental to the project.
Many students have a difficult time visualizing positive risks. A positive risk is an opportunity that may increase the probability of success, the return on investment, or the benefits of the project. They may also be ways to reduce project costs or ways to complete the project early. There may even be methods to improve project quality or overall performance. These are all examples of positive risks.
A negative risk can be easier to understand. It is the possibility that something will go wrong, a threat to the success of the project. It is important to remember that a risk is a possibility, not a fact. It is a potential problem. At GettaByte Software, there is the potential that a power outage would occur during data transfer. The potential exists that a key resource could become unavailable due to some unforeseen circumstance, like illness. Those are threats to the success of the project.
When buying a house to renovate, there are potential risks with respect to plumbing, wiring, the foundation, and so on.
A project manager needs to consider trying to make positive risks happen while trying to prevent negative ones from occurring. To do this, a project manager can take a proactive approach to risk management. This means he or she plans a risk response should it look as though the risk will become a reality. In this way, everyone knows exactly how to prepare and respond to the risk once it does become an issue.
The Risk Management Process
A project has both good and bad risks, which are referred to as positive and negative risks or opportunities and threats. For positive risks or opportunities, the project manager can choose from a range of risk responses. For threats, a project manager has a similar range of choices. The following, as described in the PMBOK® Guide, are the risk management processes.
Plan Risk Management:
· Risk Strategy
· Defines the general approach to managing risk on the project
· Methodology
· Defines the specific, tools, .
· How does the distribution mechanism control the issues address.docxalinainglis
· How does the distribution mechanism control the issues addressed in Music and TV, when in regards to race/ethnicity?
· Determine who controls the distribution of Music and TV, when in regards to race/ethnicity?
· In what ways does the controller of distribution affect the shared experience of the audience and community? Keep in mind that a community may be local, regional, national, or global. Be specific in your discussion.
.
· Helen Petrakis Identifying Data Helen Petrakis is a 5.docxalinainglis
·
Helen Petrakis Identifying Data: Helen Petrakis is a 52-year-old, Caucasian female of Greek descent living in a four-bedroom house in Tarpon Springs, FL. Her family consists of her husband, John (60), son, Alec (27), daughter, Dmitra (23), and daughter Althima (18). John and Helen have been married for 30 years. They married in the Greek Orthodox Church and attend services weekly.
Presenting Problem: Helen reports feeling overwhelmed and “blue.” She was referred by a close friend who thought Helen would benefit from having a person who would listen. Although she is uncomfortable talking about her life with a stranger, Helen says that she decided to come for therapy because she worries about burdening friends with her troubles. John has been expressing his displeasure with meals at home, as Helen has been cooking less often and brings home takeout. Helen thinks she is inadequate as a wife. She states that she feels defeated; she describes an incident in which her son, Alec, expressed disappointment in her because she could not provide him with clean laundry. Helen reports feeling overwhelmed by her responsibilities and believes she can’t handle being a wife, mother, and caretaker any longer.
Family Dynamics: Helen describes her marriage as typical of a traditional Greek family. John, the breadwinner in the family, is successful in the souvenir shop in town. Helen voices a great deal of pride in her children. Dmitra is described as smart, beautiful, and hardworking. Althima is described as adorable and reliable. Helen shops, cooks, and cleans for the family, and John sees to yard care and maintaining the family’s cars. Helen believes the children are too busy to be expected to help around the house, knowing that is her role as wife and mother. John and Helen choose not to take money from their children for any room or board. The Petrakis family holds strong family bonds within a large and supportive Greek community.
Helen is the primary caretaker for Magda (John’s 81-year-old widowed mother), who lives in an apartment 30 minutes away. Until recently, Magda was self-sufficient, coming for weekly family dinners and driving herself shopping and to church. Six months ago, she fell and broke her hip and was also recently diagnosed with early signs of dementia. Helen and John hired a reliable and trusted woman temporarily to check in on Magda a couple of days each week. Helen would go and see Magda on the other days, sometimes twice in one day, depending on Magda’s needs. Helen would go food shopping for Magda, clean her home, pay her bills, and keep track of Magda’s medications. Since Helen thought she was unable to continue caretaking for both Magda and her husband and kids, she wanted the helper to come in more often, but John said they could not afford it. The money they now pay to the helper is coming out of the couple’s vacation savings. Caring for Magda makes Helen think she is failing as a wife and mother because she no longer ha.
· Global O365 Tenant Settings relevant to SPO, and recommended.docxalinainglis
· Global O365 Tenant Settings relevant to SPO, and recommended settings
Multi Factor Authentication
Sign In Page customization
External Sharing
· Global SPO settings and recommended settings
Manage External Sharing
Site Creation Settings
· Information Architecture and Hub Site Management
Site Structure
Create and manage Hub Site
· Site Administration
Create Sites
Delete Sites
Restored Deleted Sites
Manage Site Admins
Manage Site creation
Manage Site Storage limits
Change Site Address
· Managed Metadata (Term Store)
Introduction
Setup new term group sets
Create and manage Terms
Assign roles and permission to Manage term sets
· Search
Search Content
Search Center
Crawl Site content
Remove Search results
Search Results
Manage Search Query
Manage Query Rules
Manage Query Suggestion
Manage result sources
Manage search dictionaries
· Security (identity – internal / external, and authorization – management of platform level)
Control Access of Unmanaged devices
Control Access of Network location
Authentication
Safeguarding Data
Sign out inactive users
· Governance – e.g. labels, retention, etc.
Data Classification
Create and Manage labels
· Data loss prevention
· Create and Manage security policies
· Devices Security policies
· App permission policies
· Data Governance
· Retention Policies
· Monitoring and alerting
Create and Manage Alerts
Alert Policies
· SharePoint Migration Tool
Overview
· Operational tasks for managing the health of the environment, alerting, etc.
File Activity report
Site usage report
Message Center
Service Health
· Common issue resolution and FAQ
.
· Focus on the identified client within your chosen case.· Analy.docxalinainglis
· Focus on the identified client within your chosen case.
· Analyze the case using a systems approach, taking into consideration both family and community systems.
· Complete and submit the “Dissecting a Theory and Its Application to a Case Study” worksheet based on your analysis
Helen Petrakis Identifying Data: Helen Petrakis is a 52-year-old, Caucasian female of Greek descent living in a four-bedroom house in Tarpon Springs, FL. Her family consists of her husband, John (60), son, Alec (27), daughter, Dmitra (23), and daughter Althima (18). John and Helen have been married for 30 years. They married in the Greek Orthodox Church and attend services weekly.
Presenting Problem: Helen reports feeling overwhelmed and “blue.” She was referred by a close friend who thought Helen would benefit from having a person who would listen. Although she is uncomfortable talking about her life with a stranger, Helen says that she decided to come for therapy because she worries about burdening friends with her troubles. John has been expressing his displeasure with meals at home, as Helen has been cooking less often and brings home takeout. Helen thinks she is inadequate as a wife. She states that she feels defeated; she describes an incident in which her son, Alec, expressed disappointment in her because she could not provide him with clean laundry. Helen reports feeling overwhelmed by her responsibilities and believes she can’t handle being a wife, mother, and caretaker any longer.
Family Dynamics: Helen describes her marriage as typical of a traditional Greek family. John, the breadwinner in the family, is successful in the souvenir shop in town. Helen voices a great deal of pride in her children. Dmitra is described as smart, beautiful, and hardworking. Althima is described as adorable and reliable. Helen shops, cooks, and cleans for the family, and John sees to yard care and maintaining the family’s cars. Helen believes the children are too busy to be expected to help around the house, knowing that is her role as wife and mother. John and Helen choose not to take money from their children for any room or board. The Petrakis family holds strong family bonds within a large and supportive Greek community.
Helen is the primary caretaker for Magda (John’s 81-year-old widowed mother), who lives in an apartment 30 minutes away. Until recently, Magda was self-sufficient, coming for weekly family dinners and driving herself shopping and to church. Six months ago, she fell and broke her hip and was also recently diagnosed with early signs of dementia. Helen and John hired a reliable and trusted woman temporarily to check in on Magda a couple of days each week. Helen would go and see Magda on the other days, sometimes twice in one day, depending on Magda’s needs. Helen would go food shopping for Magda, clean her home, pay her bills, and keep track of Magda’s medications. Since Helen thought she was unable to continue caretaking for both Magda and her husba.
· Find current events regarding any issues in public health .docxalinainglis
·
Find current events
regarding any issues in public health Anything about infectious diseases ( Don not pick one disease, you have you dig more infectious diseases)
· These current events can be articles, news reports, outbreaks, videos.
· Type down brief 2 sentences describing the event (don’t copy paste title)
· You should have
at least 7 diseases in
total
· No Malaria disease events, please
.
· Explore and assess different remote access solutions.Assig.docxalinainglis
· Explore and assess different remote access solutions.
Assignment Requirements
Discuss with your peers which of the two remote access solutions, virtual private networks (VPNs) or hypertext transport protocol secure (HTTPS), you will rate as the best. You need to make a choice between the two remote access solutions based on the following features:
· Identification, authentication, and authorization
· Cost, scalability, reliability, and interoperability
.
· FASB ASC & GARS Login credentials LinkUser ID AAA51628Pas.docxalinainglis
· FASB ASC & GARS Login credentials
Link
User ID: AAA51628
Password: qc3A9WS
· FASB Codification Learning Guide
· COSO Login
User ID: aaa72751
Password: JhF3a2G
Copyright 2018 Governmental Accounting Standards Board
Foreword
This content collection contains all the original pronouncements that currently constitute the body of state and local governmental accounting and financial reporting standards and guidelines. Specifically, the content collection incorporates these pronouncements:
• Governmental Accounting Standards Board (GASB) Statements, Interpretations, Concepts Statements, Technical Bulletins, and Implementation Guides issued through December 31, 2018
• National Council on Governmental Accounting (NCGA) Statements and Interpretations currently in force and NCGA Concepts Statement 1
• American Institute of Certified Public Accountants (AICPA) 1974 Industry Audit Guide and related Statements of Position continued in force when the GASB began operations
• GASB Suggested Guidelines for Voluntary Reporting issued through December 31, 2018.
Unless otherwise noted, original pronouncements in this infobase are presented in their entirety, with the exception of appendices containing codification instructions, which have been omitted. Pronouncements may include one or more nonauthoritative sections. Authoritative guidance is presented in the main body of each pronouncement. Glossaries also are considered to be authoritative. All other appendices (for example, bases for conclusions and illustrations) and summaries are nonauthoritative. In addition, the entire Suggested Guidelines for Voluntary Reporting, SEA Performance Information, is nonauthoritative.
A status page at the beginning of each pronouncement identifies subsequent changes (amendments and supersessions) to the pronouncement as well as the source of those changes. The status page also identifies (a) other pronouncements affected by that pronouncement, (b) interpretive pronouncements clarifying that pronouncement, (c) the effective date, and (d) the principal sections of the GASB Codification of Governmental Accounting and Financial Reporting Standards in which the pronouncement is incorporated.
Within each pronouncement, a shading technique is used to identify amended or superseded standards. All terms, sentences, and paragraphs that have been deleted or superseded by subsequent pronouncements are shaded. Sentences or paragraphs that have been amended by the addition of terms, sentences, or new footnotes are marked with a vertical solid bar ( | ) in the left margin alongside the amended material. When standards are amended or superseded, relevant nonauthoritative appendices are also modified to reflect those changes.
Appendix A is a reproduction of GASB Codification Appendix F, "Finding List of Original Pronouncements." It shows where each paragraph of each original pronouncement may be found in the Codification, or whether the paragraph contains.
· Due Sat. Sep. · Format Typed, double-spaced, sub.docxalinainglis
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Due:
Sat. Sep.
·
Format
: Typed, double-spaced, submitted as a word-processing document.
12 point, text-weight font, 1-inch margins.
·
·
Length
: 850 - 1000 words (approx. 3-4 pages)
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Overview
: In Unit 1 and Unit 2, we focused on ways that writers build ideas from personal memories and experiences into interesting narratives that convey significance and meaning to new audiences. In Unit 3, we have been discussing how writers invent ideas by interacting with other communities through firsthand observation and description. These relationships and discoveries can give writers insight into larger concepts or ideas that are valuable to specific communities. For this writing project, you will use firsthand observations and discoveries to write about people and the issues that are important to them. Your evidence will come from the details you observe as you investigate other people, places, and events.
Assignment
Write an ethnography essay focused on a particular group of people and the routines or practices that best reveal their unique significance as a group.
An ethnography is a written description of a particular cultural group or community. For the ethnography essay, you can follow the guidelines in the CEL, p. 110-112. Your ethnography should:
· Begin with your observations of a particular group. Plan to observe this group 2-3 times, so that you can get a better sense of their routines, habits, and practices.
o
Note: if you cannot travel to observe a group or community, plan to observe that community digitally through website documents, social media, and/or emails exchanged with group members.
· Convey insight into the characteristics that give the group unique significance.
· Provide context and background, including location, values, beliefs, histories, rituals, dialogue, and any other details that help convey the group's significance.
· Follow a deliberate organizational pattern that focuses on one or more insights about the group while also providing details and information about the group's culture and routine
As you look back over your observations and notes, remember that your essay should do more than simply relate details without any larger significance. Ethnographies also draw out the unique, interesting, and special qualities of a group or culture that help readers connect to their values or motivations. Note: Please keep in mind that writing in this class is public, and anything you write about may be shared with other students and instructors. Please only write about details that you are comfortable making public within our classroom community.
Assignment Components
In order to finish this project, we will work on the following parts together over the next few weeks:
Draft
: Include at least one pre-revised draft of your essay. The draft needs to meet the word count of 850 words and must also apply formatting requirements for the project—in other words it must be complete. Make sure that your.
· Expectations for Power Point Presentations in Units IV and V I.docxalinainglis
· Expectations for Power Point Presentations in Units IV and V
I would like to provide information about what needs to be included in presentations. Please review the rubric prior to submitting any assignment. If you don't know where to find this, please contact me.
1. You need a title slide.
2. You need an overview of the presentation slide (slide after the title slide). This is how you would organize a presentation if you were presenting it at work.
3. You need a summary slide (before the reference slide); same reason as above.
4. Please do not forget to cite on slides where you are writing about something related to what you have read. Please consider each slide a paragraph. You can cite on the slides or in the notes. If you do not cite, you will not get credit for the slide.
- Direct quotes should not be used in this presentation as they are not analysis.
5. Remember, all I can evaluate is what you submit, so please consider using notes to explain what you are writing in further detail. Bullets are great and you can use these but then provide more detail in the notes.
6. Graphics - Please include graphics/charts/graphs as this is evaluated in the rubric (quality of the presentation).
7. References - For all references, you need citations. For all citations, you need references. They must match. All must be formatted using APA requirements. Please review the Quick Reference Guide that was posted in the announcements.
Please never hesitate to email me with any questions. If you need further clarification about feedback or if you do not agree with any of the feedback, please contact me. My door is always open.
Learning Preferences of Millennials in a Knowledge-Based
Environment
Giora Hadar
University of Groningen (RuG), The Netherlands
[email protected]
Abstract: This paper discusses how understanding intergenerational knowledge transfer can improve knowledge transfer in
large organizations. The U.S. Federal Aviation Administration (FAA) risks significant loss of institutional human capital as huge
numbers of senior controllers retire. To perform their job, air traffic controllers must develop in-depth knowledge, including
tacit knowledge typically acquired over many years, so they can quickly make accurate decisions while dealing with the many
air traffic control (ATC) situations that arise. The only pool available to replace the retiring controllers is the Millennials. This
group, the best educated ever, has its own attitudes toward life, work, and training as well as technology use. Because
knowledge transfer and training involve both technology and human interaction, this paper explores not only the role of
technology but also that of intergenerational communications in both the training and operational environments of a highly
technical workplace.
Keywords: knowledge transfer, training, tacit knowledge, mentoring, mobile smart devices, communications
1. Introduction
Intergenerational knowledge transfe.
· Due Friday by 1159pmResearch Paper--IssueTopic Ce.docxalinainglis
·
Due
Friday by 11:59pm
Research Paper--
Issue/Topic:
Celebrity, Celebrity Culture and the effects on society
1500 or more words
MLA format
Must include research from
at least 4
scholarly sources (use HCC Library and GoogleScholar) I have attached 20 pdf with scholarly sources to choose from. 2 were provided from teacher Celebrity Culture Beneficial and The Culture of Celebrity. I have also attached a Word Document Research Paper Guide. Please read all the way to bottom more instructions at the bottom. Disregards Links and external cites those are the PDFs.
Celebrity
is a
popular cultural Links to an external site.
phenomenon surrounding a well-known person. Though many
celebritiesLinks to an external site.
became famous as a result of their achievements or experiences, a person who obtains celebrity status does not necessarily need to have accomplished anything significant beyond being widely recognized by the public. Some celebrities use their
fameLinks to an external site.
to reach the upper levels of social status. Popular celebrities can wield significant influence over their fans and followers. Cultural historian and film critic Neal Gabler has described the phenomenon of celebrity as a process similar to performance art in which the celebrity builds intrigue and allure by presenting a manufactured image to the public. This image is reinforced through
advertisingLinks to an external site.
endorsements, appearances at high-profile events, tabloid gossip, and
social mediaLinks to an external site.
presence.
In previous decades, celebrity status was mainly reserved for film stars,
televisionLinks to an external site.
personalities,
entertainersLinks to an external site.
, politicians, and
athletesLinks to an external site.
. Contemporary celebrities come from diverse fields ranging from astrophysics to auto mechanics, or they may simply be famous for their lifestyle or
InternetLinks to an external site.
antics. Social media platforms such as YouTube, Twitter, and Instagram provide the means for previously unknown individuals to cultivate a significant following.
Celebrification
is the process by which someone or something previously considered ordinary obtains stardom. Previously commonplace activities, such as practicing
vegetarianismLinks to an external site.
or wearing white t-shirts, can undergo celebrification when associated with a famous person or major event.
Celebrity culture
exists when stardom becomes a pervasive part of the social order,
commodified
as a commercial brand. Celebrities’ personal lives are recast as products for consumption, with a dedicated fan base demanding information and unlimited access to the celebrity’s thoughts and activities. A niche community such as a fan base can be monetized through effective marketing that links brand loyalty to the consumer’s identity. Fans may be more likely to purchase a product or attend an event if they feel that doing so strengthens their.
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
5.3 - Group Project Business Review Presentation(Just do .docx
1. 5.3 - Group Project: Business Review Presentation
(Just do section 3). 10 ppt slides with speaker notes
-Product/Country: Power Generators/U.S. to Liberia
In this assignment, you will be working in the groups assigned
in Module 4 to develop a Microsoft PowerPoint® presentation.
The group presentation will include the methods and
requirements for a global business plan consisting of the
following elements:
1.The selection of an International Business (IB)
product/service to be marketed and sold in a foreign country.(do
not do)
2.The management activities required to analyze the Internal
Environment and the expected results from country and
product/service selection.(do not do)
***3.The economic, political, legal, religious, cultural, and
business activities surrounding the External Environment for the
countries and product/service under consideration. ( This is the
section I will be doing 10 slides with speaker notes)****
REMEMBER: Your assignment can only be submitted once.
•Current APA Format
•Cite All Sources
•Speaker notes for every slide ◦How to Add Speaker Notes to
Your Slides (Links to an external site.)Links to an external site.
•Presentation should average 15 - 20 minutes in length (Judge
time as if presented to a live audience)
Pseudomonas has a plasmid containing the mer operon, which
2. includes a gene for mercuric reductase. The enzyme catalyzes
the reduction of the mercuric ion Hg2+ to the uncharged form of
mercury, Hg0 Hg2+ is quite toxic to cells, Hg0 is not.
1. What condition would induce this operon to be transcribed
and the transcript to be translated into the proteins including
mercuric reductase?
2. The protein encoded by the mer operon binds to Hg2+ outside
the cell and brings it into to the cell, why would a cell bring in
a toxin?
Two daughter cells are most likely to inherit which one of the
following from a parent cell?
3. a change in a nucleotide in mRNA, tRNA or rRNA?
4. Which of the following is NOT a method of horizontal gene
transfer? Contrast two methods of horizontal gene transfer.
conjugation, integration of a transposon, transduction or
transformtion
5. Amino acid sequences for the viral coat of HIV were
sequenced from three HIV patients. Of the amino acid
sequences shown which are most closely related?
Patient A Asn Gln Thr Ala Ala Ser Lys Asn Ile Asp Ala Leu
Patient B Asn Leu His Ser Asp Lys Ile Asn Ile Ile Leu Leu
Patient C Asn Gln Thr Ala Asp Ser Ile Val Ile Asp Ala Leu
6. The following is a code for a strand of DNA.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
DNA 3' A T A T - - - T T T _ _ _ _ _ _ _ _
11 12 13 17 18 19
mRNA C G U U G A _
tRNA U G G
6 78
Amino Acid Met ____________ ___________
______________ ____________
First fill in the blanks to complete the DNA strand, Use the
position numbers to align with mRNA, RNA and the protein.
7. What would be the effect C were substituted for T at base
(DNA) position 10?
What would be the effect if A were substituted for G at base
3. (DNA) position 11?
What would be the effect if C were inserted between bases 9
and 10?
8.
Lable a-l. Remember, you will be drawing and lableing the
DNA replication fork on Lecture Exam III.
9. What is the following region of DNA called and how does it
work? Explain the differences between an inducible and a
repressible version.
10. Fill in following concept map on types of mutations:
11. Table 8.1 on page 240 gives examples of restriction
enzymes. Restriction enzymes cut double stranded DNA at
specific sites, or palindromes. Restriction enzymes serve to cut
up phage DNA so that bacterial chromosomes are not mixed
with phage genes. Restriction enzymes do not cut bacterial
DNA because of methylation. There are two groups of
restriction enzymes based on the way the two strands of DNA
are cut. One type leaves blunt ends and the other sticky ends.
Restriction enzymes may be used to make a cut in a plasmid, a
vector, and insert a gene and use ligase to connect the gene to
the cut ends of the plasmid.
Give an example of one restristion enzyme that makes sticky
ends and one that makes blunt ends. Explain the origin of the
enzymes' names.
12. For decades researchers at Duke Medical School have
worked to engineer a polio virus to treat glioblastoma. The
strategy is to remove the genes of the virus that cause disease
4. polio and replace them with genes for a cold virus, leave the
genes that allow the virus to enter the glial cell, and the genes
that allow the virus to lyse or destroy the cell it enters for
replication. LIst and explain two 'tools of recombinant DNA
technology' explained in chapter 8 that could be used to create
this modified polio virus. Table 8.2 is a summary of t he tools
of DNA technology. p 250.
13. A large long term study on the genomic effects of PTSD
utlizes DNA microarrays to screen for changes in gene
expression of subjects in the study. Some of the preliminary
data has identified clusters of genes that change with stress.
What about this technique allows for screening of hundreds of
genes on a single glass slide? If the microarray uses cDNA,
how does cDNA differ from genomic DNA? Why must the
DNA on the slide be ssDNA?
14. Explain the function and use of reverse transciptase in
synthesizing cDNA? How does HIV use reverse transcriptase?
What drug in a cocktail (ART) used to treat AIDS targets
reverse transcriptase? p.543
15.
Please label this technique. What is the technique? Explain at
least two applications.
16. PCR utilizes taq polymerase, what is the adavantage of this
type of DNA polymerase? Why can't DNA polymerase from E.
coli be used?
17. Why are mutagens tools of recombinant DNA technology?
Give two examples of mutagens and their mode of action.
There is a market for salmon, however salmon takes three years
to mature which increases the cost of salmon. AquaBounty first
presented a fast growing fish in 1989 by combining a growth
hormone gene from the Chinook salmon and a reglatory gene
from the ocean pout to create a continouos low level of growth
hormone in the Atlantic salmon. The genetically modified
salmon grows in 18 months, may be grown in fish tanks near
5. cities thus reducing the need for air transport and tank grown
salmon have fever parasites. AquaBounty applied for FDA
approval over 25 years ago, and in 2017 meet another hurdle to
selling in the US because the FDA requires a GMO labeling, for
which a program for which is under development. Canadian
customers have bought 4.5 tons of this fish as of August 4,
2017.
18. Speculate about why genetically modified foods should be
labled for the consumer?
19. Give at least two examples of recombinant DNA techniques
that might be used to introduce genes into fish.
20. Fill in the boxes on the concept map below:
Biotechnology is the use of microbes to make practical
products. The top three justifications for manipulating the
genomes of cells are:
1. to eliminate undesirable phenotypic traits in humans,
animals, plants and microbes.
2. to combine the beneficial traits of two or more organisms to
create a new more valuable organism.
3. to create cells that synthesize products humans need.
Recombinant DNA technology employs a number to tools and
techniques to isolate genes and insert them into cells grown in
culture. The following are five tools of recombinant DNA
technology and examples of application of the tools.
Mutagens are physical and chemical agents that produce
mutation. Some mutations are beneficial, so creating a large
number of mutations increases the probability of discovering a
beneficial mutation. For example the fungus Penicillium may be
mutated to syntheisize a more effective antibiotic. Mutagens
include uv radiation and ionizing radiation. Mueller first
demonstrated the effects of X rays on Drosophilia,establishing
cause and effect between radiation and genetic changes
manifested in phenotypic changes. Chemical mutagens in
6. bacteria may be carcinogenic in humans. The Ames test utilizes
bacteria to test for mutagenic activity, and identify chemicals
that may induce cell changes that lead to cancer. Ethidium
bromide which may be used to visualize DNA on gel, is also a
mutagen because it disrupts base pairing.
1. Explain and describe one physical and one chemical mutagen
and its application.
Reverse transcriptase synthesizes cDNA from mRNA, which is
the reverse of information flow as depicted in the central dogma
of molecular biology, i.e. DNA is transcribed to RNA which is
translated to peptides. Cells may have millions of copies of
mRNA, so being able to synthesize the complementary DNA
reveals the gene that is being expressed. In addition, cDNA
contains no introns because of processing in the eukaryotic
transcription, so that cDNA may be inserted into a prokaryotic
cell and may be translated into the corresponding peptide.
2. Explain how reverse transcriptase differs from RNA
polymerase. Give one example of an application of reverse
transcriptase in recombinant DNA technology.
Synthetic nucleic acids are synthesized in vitro using enzymes
from cells. Currently there are machines that syntheized DNA or
RNA by having the sequence of bases entered tthrough a
keyborad.
3. If each letter of the keyboard represents a base in DNA, how
many keys does the keyboard require? How does this means of
DNA synthesis differ from DNA replication in the cell?
Synthetic nucleic acids were used to elucidate the genetic code,
create genes for specific proteins, synthesize probes to locate
genes in a genome, to synthesize antisense nucleic acids and to
make PCR primers.
4. What is the genetic code, how might a synthetic nucleotide be
used to determine which amino acid corrresponds to which
codon?
5. Give an example of a probe with a flourescent tag that will be
used to locate a gene associated with an aggressive form of
breast cancer.
7. Restiction enzymes cut dsDNA at restriction sites, or
palindromes. Examples of restriction enzymes are EcoRI and
HindIII. There are hundreds of restriction enzymes isolated
from bacteria and used to cut DNA at predictable sites. One
type of restriction enzyme cuts dsDNA to make blunt ends and
the other type creates sticky ends.
6. Compare and contrast blunt and sticky ends, and give one
example of each type of restriction enzyme.
Vectors insert DNA into a new cell so that the cell acquires a
new phenotype. Vectors are pieces of DNA that are small
enough to manipulate in a lab, survive inside the new cell,
contain a recognizalbe genetic marker and carry the gene
regions necessary to ensure the gene is transcribed and
translated. Examples of vectors are plasmids, viruses and
transposons.
7. Explain one risk of a viral vector that inserts itself into a
necessary gene and causes a mutation.
Gene library is a collection of cells or viruses, in which each
member carries a portion of a given organism's genome. Gene
libraries provide a ready
Techniques of recombinant DNA technology include the
polymerase chaing reaction (PCR) developed by Cary Mullis.
PCR has three steps that are repeated while the same sample of
primers, target gene, taq polymerase and nucleotides are cycled
through three different temperatures in a microfuge tube. Gel
electrophoresis separated fragments of molecules by size, shape
and charge. DNA microarrays are able to monitor thousands of
genes on one plate. Applications of these techniques include
diagnostics or vaccine design.
8. Explain how a subunit vaccine is designed using genes from
a pathogen and a viral vector? p502-504.
One proposed way to stop the spread of arboviruses by
mosquitoes is to vaccinate the mosquito. This type of vaccine
is a transmission blocking vaccine (TBV). One typeof TBV
vaccinates the human so that a blood meal from a vaccinated
human would prevent the mosquito from being infected.
8. Another strategy is to use the gene drive known as Crispr-Cas9
which would edit the mosquito genome to confer resistance to
diseases mosquitoes transmit to humans, and is very targeted.
This requires wild type mosquitoes to mate with lab mosquitoes
that have been genetically modified. Some data would indicate
the lab mosquitoes are not competitve with wild type males.
9. Explain one advantage and one disadvantage of the TBV
approach for controlling the spread of arboviruses.
10. Does the public have any control over the use of gene drives
to alter species in the ecosystem for public health concerns?