Significance The Bradford assay is a widely used method for.pdf

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Significance: The Bradford assay is a widely used method for protein quantification due to its simplicity, rapidity, and high sensitivity (Khan et al., 2016). Unlike other protein quantification methods, the Bradford assay does not require prolonged incubation or extensive sample preparation. This assay is also highly reproducible and can quantify protein concentrations in various samples, including purified proteins, cell extracts, and biological fluids (Amarowicz et al., 2017; Thavarajah et al., 2018). Application: The Bradford assay has numerous applications in biochemical and molecular biology research. It can quantify protein concentrations in various samples, including cell culture media, protein solutions, and biological fluids such as blood and urine (Thavarajah et al., 2018). The Bradford assay is also used in protein purification and protein- protein interaction studies (Amarowicz et al., 2017). Additionally, this assay is used in clinical applications to diagnose diseases that affect protein concentrations in the body, such as liver disease and kidney disease (Shimoda et al., 2013)..

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