A brief introduction to microscopy including optical micoscopes and electron microscopes.

1. Introduction to Microscopy
Christina Huang
2018/02/28

2. Outlines
Microscopy
Optical microscope
Electron microscope
Other types?

3. Microscopy
 Microscopy: Use microscopes to view objects
that cannot be seen with the naked eye (100 µm)
 Macroscopy: objects can be seen by the naked eye
 Telescope

4. Optical microscopes
 Microscope use visible light to magnify images
 Limit size: 0.2 µm
 Abbe diffraction limit
standard optical microscopes has resolution limit of the half length of light
wavelength
*wavelength of the visible light: 0.4-0.7 µm
https://www.microscopyu.com/techniques/super-resolution/the-diffraction-barrier-in-optical-microscopy

5. Electron microscopes
 Scanning electron microscope (SEM):
produces images of a sample by scanning it with a focused beam of electrons
 Wavelength of an electron is shorter than that of visible light photons
 higher resolving power

6. BSE-SEM
 Backscattered-Electron (BSE)
https://www.azom.com/article.aspx?ArticleID=14309
Detector

7. SE-SEM
 Secondary Electrons (SE)
 Electrons ejected by electrons injected
 originate from surface or near-surface regions
Detector

8. Backscattered electronSecondary electron

9. TEM
 Transmission electron microscopy (TEM)
 transmitted electrons
 ultra-thin section of specimen
 inner structure information
 Semi-conductor
Detector

10. Other types?

11. AFM
 Atomic force microscope
 Only morphology of the surface

12. NIKON’S SMALL WORLD
https://www.nikonsmallworld.com/

13. THANKS 