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Microscope
• Micro (small)
• Scope (view)
Three common types:
1. Compound Light
2. Stereo
3. Electron (2 versions)
1. Compound Light
• Most common
• 2 lenses focusing at the same time
1. Ocular
2. Objective
• 2D image
• High magnificatio...
Parts of the microscope
Arm
Base
Term: Magnification
• Ratio of image size to actual size
• Ocular: 10X
(“X” means times; for example, the ocular
magnifies...
Field of View
Amount of object you can see
• Decreases with increasing magnification
Depth of Focus
Thickness of specimen in focus at a given
magnification
• Decreases with increasing magnification
• Lab say...
Inversion Phenomenon
• Objects appear upside down and
backwards in the compound microscope
• We will use prepared letter e...
2. Stereomicroscope
• Used to get a better look at larger objects
• 3D image
• Specimen not mounted on a slide
• Low magni...
3. Electron Microscope
• Uses electron streams focused by
magnets to view specimens, not light
• 2 types
1. Scanning
2. Tr...
Scanning Electron Microscope
• 3D images
• 1,000-10,000x magnification
• Resolution of 5 nanometers
pollen
microscope
Transmission Electron Microscope
• 2D
• 10,000-100,000 magnification
mitochondria
Wet mount
• Way to create a simple slide
• Need a clean slide and a coverslip
• Procedure
– Place specimen on slide
– Add ...
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Microscope Biology I Lab Slide 1 Microscope Biology I Lab Slide 2 Microscope Biology I Lab Slide 3 Microscope Biology I Lab Slide 4 Microscope Biology I Lab Slide 5 Microscope Biology I Lab Slide 6 Microscope Biology I Lab Slide 7 Microscope Biology I Lab Slide 8 Microscope Biology I Lab Slide 9 Microscope Biology I Lab Slide 10 Microscope Biology I Lab Slide 11 Microscope Biology I Lab Slide 12
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Microscope Biology I Lab

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Biology I Lab instructions: Microscope

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Microscope Biology I Lab

  1. 1. Microscope • Micro (small) • Scope (view) Three common types: 1. Compound Light 2. Stereo 3. Electron (2 versions)
  2. 2. 1. Compound Light • Most common • 2 lenses focusing at the same time 1. Ocular 2. Objective • 2D image • High magnification through light absorption
  3. 3. Parts of the microscope Arm Base
  4. 4. Term: Magnification • Ratio of image size to actual size • Ocular: 10X (“X” means times; for example, the ocular magnifies something 10 times) • 4 objectives – Scan: 4X – Low Power: 10X – High Power: 40X – Oil immersion: 100x • Multiply ocular x objective to get overall magnification
  5. 5. Field of View Amount of object you can see • Decreases with increasing magnification
  6. 6. Depth of Focus Thickness of specimen in focus at a given magnification • Decreases with increasing magnification • Lab says to make your own slide-we will use a prepared thread slide
  7. 7. Inversion Phenomenon • Objects appear upside down and backwards in the compound microscope • We will use prepared letter e slides
  8. 8. 2. Stereomicroscope • Used to get a better look at larger objects • 3D image • Specimen not mounted on a slide • Low magnification • Uses visible light through light scattering
  9. 9. 3. Electron Microscope • Uses electron streams focused by magnets to view specimens, not light • 2 types 1. Scanning 2. Transmission
  10. 10. Scanning Electron Microscope • 3D images • 1,000-10,000x magnification • Resolution of 5 nanometers pollen microscope
  11. 11. Transmission Electron Microscope • 2D • 10,000-100,000 magnification mitochondria
  12. 12. Wet mount • Way to create a simple slide • Need a clean slide and a coverslip • Procedure – Place specimen on slide – Add a drop of water – Place coverslip over specimen at an angle to avoid air bubbles – Wipe away excess water – View under microscope • Today: pond water wet mount
  • JennyJones98

    Nov. 25, 2021
  • NathalieMoreno12

    Jun. 10, 2019
  • HalaO1

    Mar. 4, 2017
  • HalaO1

    Mar. 2, 2017

Biology I Lab instructions: Microscope

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