Basics SIMPLE MICROSCOPE BRIGHTFIELD MICROSCOPE DARK-FIELD MICROSCOPE STEREO ZOOM MICROSCOPE PHASE CONTRAST MICROSCOPE FLUORESCENT MICROSCOPE TRANSMISSION ELECTRON MICROSCOPE SCANNING ELECTRON MICROSCOPE SOURCE OF ILLUMINATION SAMPLEVISUALIZATION CONDENSER ADDITIONAL SUPPORT SYSTEM SPECIMEN STAINING MAGNIFICATION POWER RESOLUTION APPLICATION

1. COMPARATIVE ACCOUNT ON DIFFERENT TYPES OF MICROSCOPES
SIMPLE
MICROSCOPE
BRIGHT-
FIELD
MICROSCOPE
DARK-FIELD
MICROSCOPE
STEREO
ZOOM
MICROSCOPE
PHASE
CONTRAST
MICROSCOPE
FLUORESCENT
MICROSCOPE
TRANSMISSION
ELECTRON
MICROSCOPE
SCANNING
ELECTRON
MICROSCOPE
SOURCE OF
ILLUMINATION
Day light /
Electric lamp
Day light /
Electric lamp
Electric lamp Day light /
Electric lamp
Electric lamp Mercury Arc
Lamp
Electron Gun Electron Gun
SAMPLE
VISUALIZATION
Naked eye Naked eye /
Screen
Naked eye /
Screen
Naked eye Naked eye /
Screen
Naked eye /
Screen
Photographic
film
Photographic
film
CONDENSER ----- Abbes
condenser
Dark-field
condenser
----- Simple
condenser
Dark-field
condenser
Electromagnetic
condenser lens
system
Electromagnetic
condenser lens
system
ADDITIONAL
SUPPORT
SYSTEM
----- ----- Dark-field
Ring
2 ocular lens Annular
diaphragm,
Phase plate,
Phase ring.
Heat filter,
Exciter filter,
Dichroic mirror,
Barrier filter
Intermediate
lens,
projector lens,
fluorescent
screen
Beam deflector,
Scan generator,
Scintillation
detector, video
Screen
SPECIMEN
STAINING
Not necessary Necessary Not necessary Not necessary Not necessary Necessary -
Fluorescent
dyes
Dehydration,
Fixation,
Ultra-sectioning,
Positive/Negative
Staining
Dehydration,
Shadow Casting,
Surface replica
MAGNIFICATION
POWER
10 x 1000 - 2000 x 1000 - 2000 x ~200 x 1000 - 2000 x 1000 - 2000 x 50,000 -
10,00,000 X
1,00,000 -
1,00,00,000 x
RESOLUTION 1000 nm 100 - 200 nm 100 - 200 nm ~1000 nm 100 - 200 nm 100 - 200 nm 50 - 100 nm 1 - 10 nm
APPLICATION For
Dissection
To study
morphology of
microbes.
To study
morphology of
live specimen.
For Dissection To study the
internal
structures of
live-microbes.
Useful in
diagnostic and
identification of
microbes.
To study ultra-
structure and
anatomy of cell.
To study surface
architecture of
microbes, etc…