Attuluri Vamsi Kumar, MLT, SMAS, Galgotais Unversity.

1. Module- 5
Topic name
Small Pox
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV, COVID-19
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9
hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies,
Arbo viruses, Measles, Mumps, Rubella, HIV, COVID-19
Unit 4

4. Unit-5 DNA Viruses
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster,
Hepatitis, Adeno virus
Unit - 5
Unit - 5

5. Families of viruses
DNA Viruses RNA Viruses
HAP3
Herpes viridae
1. Alpha – HS1, HS2, EBV
2. Beta – CMV
3. Gama -VZ
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
PR2OCBT
H H
PCR2MMR3 – IH
1. Polio virus
2. Corona virus
3. Rhino virus
4. Rota virus
5. Measles virus
6. Mumps virus
7. Rubella
8. Rabis
9. Influenza
10. HIV
AIDS

6. Families of viruses
DNA Viruses
HAP3
Herpes viridae
1. Alpha – HS1, HS2, EBV
2. Beta – CMV
3. Gama -VZ
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
H

7. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
Mechanism + incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

8. INTRODUCTION

22. Timeline of small pox

28. Notes

29. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
Mechanism + incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

30. Introduction
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

31. Classification
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

32. Morphology
1. Size- 300 ˣ 200 ˣ 100nm
2. Shape- Brick shaped
3. Symmetry- Complex (dumbbell
shaped)
4. Genome- dsDNA
5. Envelop – Present
6. Special proteins – Lateral Bodies
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

33. Susceptibility & Resistance
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

34. Cultivation
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

42. Pathogenesis
Mode of Transmission

44. Pathogenesis

48. Eradication of smallpox