One of the first plausible models to account for the preceding observations was
formulated by Robin Holliday.
The key features of the Holliday model are the formation of heteroduplex DNA; the
creation of a cross bridge; its migration along the two heteroduplex strands,
termed branch migration; the occurrence of mismatch repair; and the
subsequent resolution, or splicing, of the intermediate structure to yield different
typesof recombinant molecules.
1. MOLECULAR MECHANISM OF CROSSING OVER
HOLIDAY MODEL
One of the first plausible models to account for the preceding observations was
formulated by Robin Holliday.
The key features of the Holliday model are the formation of heteroduplex DNA; the
creation of a cross bridge; its migration along the two heteroduplex strands,
termed branch migration; the occurrence of mismatch repair; and the
subsequent resolution, or splicing, of the intermediate structure to yield different
typesof recombinant molecules.
1. ALIGNMENT OF TWO HOMOLOGOUS DNA MOLECULES
Two homologous double helices, Each pair represents a chromatid and the two pairs
represent two nonsister chromatids. The helices are aligned so that the bottom strand
of the first helix has the same polarity as the top strand of the second helix.
The two homologous double helices are aligned, although note that they have been
rotated so that the bottom strand of the first helix has the same polarity as the top
strand of the second helix (5′ → 3′ in this case).
2. INTRODUCTION OF NICKS/BREAKS IN THE DNA
The endonuclease cleaves the two strands that have the same polarity. Two parallel
or two antiparallel strands are cut.
Formation of Single strand nicks on two strands
3. STRAND INVASION/DISPLACEMENT
The ssDNA invade the corresponding homologgous DNA duplex.
Initial short regions of base pairing are formed between the two recombing BDNA
molecuules. This event is called as Strand Invasion
4. HETERO DUPLEX FORMATION
As a result of strand invasion, regions of new duplex DNA are generated. Which often
contains mismatched base pairs, is called Heteroduplex DNA
5. FORMATION OF HOLLIDAY JUNCTION
The free ends leave their original complementary strands and undergo hydrogen
bonding with the complementary strands in the homologous double helix.
Ligation produces partially heteroduplex double helix is a crucial intermediate
in recombination, and has been termed the Holliday structure.
6. BRANCH MIGRATION
The Holliday structure creates a cross bridge, or branch, that can move, or migrate,
along the heteroduplex
This phenomenon of branch migration is a distinctive property of the Holliday
structure, portrays a more realistic view of this structure as it might appear during
branch migration.
Branch migration, the movement of the crossover point between DNA complexes.
2. 7. RESOLUTION OF HOLLIDAY JUNCTION
The Holliday structure can be resolved by cutting and ligating either the two
originally exchanged strands or the originally unexchanged strands.
Resolution of the structure shown in part c can proceed in two ways, depending on
the points of enzymatic cleavage, yielding the structures
The former generates a pair of duplexes that are parental, except for a stretch in the
middle containing one strand from each parent.
If the two parents had different alleles in this stretch, as indicated here, then
the DNA will be heteroduplex.
The latter resolution step generates two duplexes that are recombinant, with a stretch
of heteroduplex DNA.
The Holliday model also postulated that the heteroduplex DNA mismatches can be
repaired by an enzymatic correction system that recognizes mismatches and excises
the mismatched base from one of the two strands, filling in the excised base with the
correct complementary base.