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WELCOME
Doctoral Seminar-I
Biofortification in Fruit Crops
Presented By
Kadge Nishant Ramesh
Ph.D. II Year Student (Fruit Science)
Presentedto
Department of Fruit Science
Faculty of Horticulture,
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
Introduction
Nutritional Situation
Minerals are so important for human body why ?
What is Biofortification?
What is Biofortified crops?
Advantages and Constraints
Government Programmes
Methods of Biofortification
Future Challenges
Conclusion
Outline of Seminar
BIOFORTIFICATION
Undernourished people in world
Biofortification in Fruit Crops
Biofortification in Fruit Crops
The News From-Times of India Article ,4th
August, 2014
The News From-The National Network
11th Dec 2015
Biofortification in Fruit Crops
MIcroelement are so important FOR HUMAN BODY, why ?
 Deficiency affect blindness, birth defects, mental health and child survival.
 Deficiency increase mortality.
 Deficiency of vitamin A lead to blindness.
 Iron needed for physical and cognitive development.
 Zinc deficiency causes stunting in children.
 Women and young children most affected.
 1 out of 3 people in developing countries suffers.
 More than 40% children under five are stunted while an estimated 44% of
the same age group are at risk of zinc deficiency.
Biofortification in Fruit Crops
Deficiency Symptoms IN HUMAN
Vitamin A
Eye sight problem, Reduce
immunity, Night blindness,
Retard growth, Dry skin
Iron (Fe)
Anaemia, Chest pain, Reduced
immunity Dizziness, Headache,
Weakness, Glossitis, Frequent
worm infestation
Zinc (Zn)
Reduced growth, Immunity, Loss
of apetite, Weight loss, Skin
Dryness, Rashes
Biofortification in Fruit Crops
Impact of
Malnutrition
Low Mood
Weight Loss
Low
Energy
Muscle
Wasting
Increase Risk
for Fractures
Increase Risk
for Hospital
Additions
Confusion
Infections
Reduced
Independence
Increase Risk
of Falls
Reduced
Mobility
Biofortification in Fruit Crops
DIETARY REFERENCE INTAKES (DRIs)
Nutrients Intake (mg/day)
Protein 0.66-0.88
Vitamin A 500-630
Calcium 500-1000
Iodine 73-95
Iron 5-8.1
Magnesium 200-350
Selenium 35-45
Zinc 6.8-9
Food and Nutrition Board, Institute of Medicine, National Academics, 2013
Biofortification in Fruit Crops
Richest Source of Vitamins and Minerals
Sources Fruit Content Fruit Content
Vitamin A Mango 4800 IU/100g Papaya 2020 IU/100g
Vitamin B1 Cashew 630mg/100g Walnut 450mg/100g
Vitamin B2 Bael 1191mg/100g Papaya 250mg/100g
Vitamin C Barbados cherry
1000-
4000mg/100g
Aonla 600mg/100g
Carbohydrate Apricot 72.81% Karonda 67.10%
Protein Cashew 21.20% Almond 20.88%
Calcium Litchi 0.21% Karonda 0.16%
Phosphorus Almond 0.49% Cashew 0.45%
Iron Karonda 39.1% Dates 10.6%
Source- Jitendra Singh, 2004Biofortification in Fruit Crops
What is Bio-fortification ?
• Greek word “bios” means “life” and Latin word “fortificare” means
“make strong”.
Defination
The process by which the nutritional quality of food crops is improved
through agronomic practices, conventional plant breeding or modern
biotechnology.
High yielding
crop varieties
x
High Nutrient
crop varieties
Biofortified
food crop
=
Biofortification in Fruit Crops
What is Bio-fortified crops ?
Discovery
 Identified target
population.
 Set nutrient target
level.
 Screen germplasm
and gene
discovery
Development
 Test the performance
of new crop varieties.
 Measure nutrient
retention in crop.
Dissemination
 Develop strategies to
disseminate the seed
or plant material.
 Promote marketing
and consumption of
biofortified crops
1 2 3
Biofortification in Fruit Crops
WHY BIOFORTIFICATION?
Source-McLean et al., 2009
 Increase bioavailable concentrations of essential nutrients in edible
portions of food crops.
 Provides a truly feasible means of reaching malnourished populations
in relatively remote rural areas.
 Deliver naturally-fortified foods to population groups with limited
access to commercially-marketed fortified foods.
 WHO estimated that biofortification could help cure 2 billion people
suffering from iron deficiency induced anaemia.
Biofortification in Fruit Crops
How it Differ From Fortification ?
Biofortification differ from ordinary fortification because it focuses
on making plant foods more nutritious as the plant are growing, rather
than having nutrients added to the foods when they are being processed.
Biofortification in Fruit Crops
+ Nutrient Food Processing
More
Nutrient
Consumed
1. Traditional Fortification
Fruits
2. Biofortification
+
Selective Breeding
Plant producing low
level of nutrients
-OR-
Genetic Modification
Seeds
+
Genes
Insert gene to
increase plant
nutrient production
Plant producing High
levels of nutrients
Dietary
supplements
Varied plant
based diet
Biofortification in Fruit Crops
Commercially Available Fortified Foods
Biofortification in Fruit Crops
Advantages of Biofortification
 Increase in nutrient value. i.e. bioavailable Vitamin A and B6; Fe; Protein and
Zinc.
 Reduced adult and child micronutrient caused mortality.
 Reduced dietary deficiency diseases. eg. Blindness in children, diarrhoea,
anaemia, etc.
 It is this multiplier aspect of plant breeding across time and distance that
makes it so cost-effective.
 Once in place, the biofortified crop system is highly sustainable.
 Healthier populations with strong and quick immune response to infections.
 Biofortification provides a truly feasible means of reaching malnourished
populations in relatively remote rural areas.
Cont…..Biofortification in Fruit Crops
 Increase in Yield. i.e. Biofortified crops enhance abiotic stress tolerance.
 After the one-time investment is made to develop seeds that fortify themselves,
recurrent costs are low and germplasm may be shared internationally.
 The inbuilt resistance to pests, weeds, and diseases makes them more
environment friendly as they require less herbicides and pesticides.
 Direct health advantage for farmers is a result of less insecticide exposure
during spraying operations.
 Although the seed is more expensive, the overall cost of production is low
because of reduced inputs of machinery, fuel, and chemical pesticides.
Biofortification in Fruit Crops
Constraints of Biofortification
 High production cost. i.e. equipment, technology, patenting, etc.
 Potential negative interaction of biofortified crop/ non GM crop causing loss of
wild type varieties.
 Low substantial equivalence. i.e. inability to provide high micronutrient and
protein content compared to supplements.
 For rural populations have limited access and resources to purchase
biofortified crops.
 The genetic engineering methods used may compromise immunity in human.
i.e. introduced increase risk allergecity.
 Monoculture will wipe out diversity.
Biofortification in Fruit Crops
Biofortification and zero hunger challenge
The second Global Biofortification Conference may recommend to the UN
that a year between 2018-2020 may be observed as the International Year of
Biofortified and Underutilized Crops. This will be an important step in the meeting
the Zero Hunger Challenge by 2025, since biofortified crops help to address
simultaneously under and malnutrition.
To celebrate the International year of Family Farming and Asia Pacific
Conference was held at MSSRF, Chennai on August, 2014. With one of the major
objectives of the conference is the launch of Zero Hunger Programme in India.
ICN2 Second International Conference on Nutrition with theme “Better
Nutrition Better Lives” was shows global awareness towards biofortification.
Source- Kigali, 2014
Biofortification in Fruit Crops
Methods of biofortification
Agronomical
Biofortification
Conventional
Breeding
Genetic Modification
Biofortification in Fruit Crops
Comparison of existing practices
Sr.
No.
Agronomical
Biofortification
Conventional
Breeding
Genetic Modification
Advantages
Simple,
inexpensive, Rapid
enhancement
Uses intrinsic
properties of crops
Rapid, unconstrained by
gene pool, targeted
expression in edible
organs, applicable
directly to elite cultivars.
Disadvantages
Only work with
minerals, very
dependent on crop
and cultivar; not
possible to target
edible organ
Depends on
existing gene pool;
takes long time;
traits might need to
be introgressed
from wild relatives,
possible
intellectual
property
constraints.
Regulatory landscape,
political and
socioeconomic issues
relevant to transgenic
plants, possible
intellectual property
constraints.
Source-Zhu et al., 2007Biofortification in Fruit Crops
Future challenge
 Consumer preference.
 Production of crops for human nutrition with increase iron
concentration.
 Promoting large scale prospective studies on assessing the effects of
nutrient enhancement in major crops in relieving malnutrition and
other associated health problems.
 Improving the efficiency with which minerals are mobilized in the soil.
 Enhancing the mineral uptake efficiency of the important crops.
 Expanding the understanding of mineral accumulation and the
transport within the plant body.
 The biggest hurdle in the commercial use of GM crops is the regulatory
approval process which is very expensive and time consuming.
Biofortification in Fruit Crops
Biofortification in Banana
Variety Name
Country of
Origin
Genome- Sub
group
Fruit Ripening
stage
Total
Carotenoid
content
Apantu Ghana AAB-Plantain
Unripe
Ripe
46.83 ppm
100.71 ppm
Bira
Papua New
Guinea
AAB-Pacific
plantain
Unripe
Ripe
43.42ppm
106.38 ppm
Pelipita Philippines ABB-Plantain
Unripe
Ripe
25.35 ppm
17.44 ppm
To’o
Papua New
Guinea
AA-Desert
Unripe
Ripe
5.60ppm
77.69ppm
Source- Ekesa, 2015
Fortified Banana
Normal Banana
Biofortification in Fruit Crops
Biofortification in pomegranate
Solapur Lal- Hybrid Ganesh
5.6-6.1 mg/100g Iron 2.7-3.2 mg/100g
0.64-0.69 mg/100g Zinc 0.50-0.54 mg/100g
19.4-19.8 mg/100g Vitamin-C 14.2-14.6 mg/100g
Solapur Lal- Hybrid
 Fruit yield: 23.0-27.0 t/ha.
 Developed by ICAR-National Research Centre on Pomegranate,
Solapur.
 Adaptation: Semi-arid regions of the country.
Source- ICAR,2017
Biofortification in Fruit Crops
NRCP H-6 Bhagwa
6.10 mg/100g Iron 2.8 mg/100g
0.69 mg/100g Zinc 0.52 mg/100g
Biofortification in pomegranate
A group of scientists from National Research Centre for Pomegranate(NRCP),
Solapur, in collaboration with Indian Institute Horticulture Research (IIHR), Bengaluru,
has developed a new hybrid pomegranate variety called "NRCP H-6", which has a high
content of iron and zinc compared to the existing commercial variety Bhagwa.
The news from- Times of India, 10th April 2017Biofortification in Fruit Crops
Conclusion
 Biofortification is a cost effective, feasible means of reaching populations who
may have limited availability and access to diverse diets, supplements, or
commercially fortified foods.
 A one-time investment in developing for micronutrient rich varieties for
farmers to grow for years to come, and the same varieties can be evaluated in
other target geographies with similar agro-ecological conditions, thus
multiplying the benefit of the initial investment.
 Biofortification is one solution among many that are needed to solve the
complex problem of micronutrient deficiency, and it complements existing
interventions.
 For biofortification it is vast scope to develop the biofortified varieties in fruits
crops like; guava, annona spp., ber, papaya, jackfruit, pineapple, etc.
Biofortification in Fruit Crops
Eat well
Live well
Be well
Biofortification in fruit crops

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Biofortification in fruit crops

  • 2. Doctoral Seminar-I Biofortification in Fruit Crops Presented By Kadge Nishant Ramesh Ph.D. II Year Student (Fruit Science) Presentedto Department of Fruit Science Faculty of Horticulture, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
  • 3. Introduction Nutritional Situation Minerals are so important for human body why ? What is Biofortification? What is Biofortified crops? Advantages and Constraints Government Programmes Methods of Biofortification Future Challenges Conclusion Outline of Seminar BIOFORTIFICATION
  • 4. Undernourished people in world Biofortification in Fruit Crops
  • 6. The News From-Times of India Article ,4th August, 2014 The News From-The National Network 11th Dec 2015 Biofortification in Fruit Crops
  • 7. MIcroelement are so important FOR HUMAN BODY, why ?  Deficiency affect blindness, birth defects, mental health and child survival.  Deficiency increase mortality.  Deficiency of vitamin A lead to blindness.  Iron needed for physical and cognitive development.  Zinc deficiency causes stunting in children.  Women and young children most affected.  1 out of 3 people in developing countries suffers.  More than 40% children under five are stunted while an estimated 44% of the same age group are at risk of zinc deficiency. Biofortification in Fruit Crops
  • 8. Deficiency Symptoms IN HUMAN Vitamin A Eye sight problem, Reduce immunity, Night blindness, Retard growth, Dry skin Iron (Fe) Anaemia, Chest pain, Reduced immunity Dizziness, Headache, Weakness, Glossitis, Frequent worm infestation Zinc (Zn) Reduced growth, Immunity, Loss of apetite, Weight loss, Skin Dryness, Rashes Biofortification in Fruit Crops
  • 9. Impact of Malnutrition Low Mood Weight Loss Low Energy Muscle Wasting Increase Risk for Fractures Increase Risk for Hospital Additions Confusion Infections Reduced Independence Increase Risk of Falls Reduced Mobility Biofortification in Fruit Crops
  • 10. DIETARY REFERENCE INTAKES (DRIs) Nutrients Intake (mg/day) Protein 0.66-0.88 Vitamin A 500-630 Calcium 500-1000 Iodine 73-95 Iron 5-8.1 Magnesium 200-350 Selenium 35-45 Zinc 6.8-9 Food and Nutrition Board, Institute of Medicine, National Academics, 2013 Biofortification in Fruit Crops
  • 11. Richest Source of Vitamins and Minerals Sources Fruit Content Fruit Content Vitamin A Mango 4800 IU/100g Papaya 2020 IU/100g Vitamin B1 Cashew 630mg/100g Walnut 450mg/100g Vitamin B2 Bael 1191mg/100g Papaya 250mg/100g Vitamin C Barbados cherry 1000- 4000mg/100g Aonla 600mg/100g Carbohydrate Apricot 72.81% Karonda 67.10% Protein Cashew 21.20% Almond 20.88% Calcium Litchi 0.21% Karonda 0.16% Phosphorus Almond 0.49% Cashew 0.45% Iron Karonda 39.1% Dates 10.6% Source- Jitendra Singh, 2004Biofortification in Fruit Crops
  • 12. What is Bio-fortification ? • Greek word “bios” means “life” and Latin word “fortificare” means “make strong”. Defination The process by which the nutritional quality of food crops is improved through agronomic practices, conventional plant breeding or modern biotechnology. High yielding crop varieties x High Nutrient crop varieties Biofortified food crop = Biofortification in Fruit Crops
  • 13. What is Bio-fortified crops ? Discovery  Identified target population.  Set nutrient target level.  Screen germplasm and gene discovery Development  Test the performance of new crop varieties.  Measure nutrient retention in crop. Dissemination  Develop strategies to disseminate the seed or plant material.  Promote marketing and consumption of biofortified crops 1 2 3 Biofortification in Fruit Crops
  • 14. WHY BIOFORTIFICATION? Source-McLean et al., 2009  Increase bioavailable concentrations of essential nutrients in edible portions of food crops.  Provides a truly feasible means of reaching malnourished populations in relatively remote rural areas.  Deliver naturally-fortified foods to population groups with limited access to commercially-marketed fortified foods.  WHO estimated that biofortification could help cure 2 billion people suffering from iron deficiency induced anaemia. Biofortification in Fruit Crops
  • 15. How it Differ From Fortification ? Biofortification differ from ordinary fortification because it focuses on making plant foods more nutritious as the plant are growing, rather than having nutrients added to the foods when they are being processed. Biofortification in Fruit Crops
  • 16. + Nutrient Food Processing More Nutrient Consumed 1. Traditional Fortification Fruits 2. Biofortification + Selective Breeding Plant producing low level of nutrients -OR- Genetic Modification Seeds + Genes Insert gene to increase plant nutrient production Plant producing High levels of nutrients Dietary supplements Varied plant based diet Biofortification in Fruit Crops
  • 17. Commercially Available Fortified Foods Biofortification in Fruit Crops
  • 18. Advantages of Biofortification  Increase in nutrient value. i.e. bioavailable Vitamin A and B6; Fe; Protein and Zinc.  Reduced adult and child micronutrient caused mortality.  Reduced dietary deficiency diseases. eg. Blindness in children, diarrhoea, anaemia, etc.  It is this multiplier aspect of plant breeding across time and distance that makes it so cost-effective.  Once in place, the biofortified crop system is highly sustainable.  Healthier populations with strong and quick immune response to infections.  Biofortification provides a truly feasible means of reaching malnourished populations in relatively remote rural areas. Cont…..Biofortification in Fruit Crops
  • 19.  Increase in Yield. i.e. Biofortified crops enhance abiotic stress tolerance.  After the one-time investment is made to develop seeds that fortify themselves, recurrent costs are low and germplasm may be shared internationally.  The inbuilt resistance to pests, weeds, and diseases makes them more environment friendly as they require less herbicides and pesticides.  Direct health advantage for farmers is a result of less insecticide exposure during spraying operations.  Although the seed is more expensive, the overall cost of production is low because of reduced inputs of machinery, fuel, and chemical pesticides. Biofortification in Fruit Crops
  • 20. Constraints of Biofortification  High production cost. i.e. equipment, technology, patenting, etc.  Potential negative interaction of biofortified crop/ non GM crop causing loss of wild type varieties.  Low substantial equivalence. i.e. inability to provide high micronutrient and protein content compared to supplements.  For rural populations have limited access and resources to purchase biofortified crops.  The genetic engineering methods used may compromise immunity in human. i.e. introduced increase risk allergecity.  Monoculture will wipe out diversity. Biofortification in Fruit Crops
  • 21. Biofortification and zero hunger challenge The second Global Biofortification Conference may recommend to the UN that a year between 2018-2020 may be observed as the International Year of Biofortified and Underutilized Crops. This will be an important step in the meeting the Zero Hunger Challenge by 2025, since biofortified crops help to address simultaneously under and malnutrition. To celebrate the International year of Family Farming and Asia Pacific Conference was held at MSSRF, Chennai on August, 2014. With one of the major objectives of the conference is the launch of Zero Hunger Programme in India. ICN2 Second International Conference on Nutrition with theme “Better Nutrition Better Lives” was shows global awareness towards biofortification. Source- Kigali, 2014 Biofortification in Fruit Crops
  • 23. Comparison of existing practices Sr. No. Agronomical Biofortification Conventional Breeding Genetic Modification Advantages Simple, inexpensive, Rapid enhancement Uses intrinsic properties of crops Rapid, unconstrained by gene pool, targeted expression in edible organs, applicable directly to elite cultivars. Disadvantages Only work with minerals, very dependent on crop and cultivar; not possible to target edible organ Depends on existing gene pool; takes long time; traits might need to be introgressed from wild relatives, possible intellectual property constraints. Regulatory landscape, political and socioeconomic issues relevant to transgenic plants, possible intellectual property constraints. Source-Zhu et al., 2007Biofortification in Fruit Crops
  • 24. Future challenge  Consumer preference.  Production of crops for human nutrition with increase iron concentration.  Promoting large scale prospective studies on assessing the effects of nutrient enhancement in major crops in relieving malnutrition and other associated health problems.  Improving the efficiency with which minerals are mobilized in the soil.  Enhancing the mineral uptake efficiency of the important crops.  Expanding the understanding of mineral accumulation and the transport within the plant body.  The biggest hurdle in the commercial use of GM crops is the regulatory approval process which is very expensive and time consuming. Biofortification in Fruit Crops
  • 25. Biofortification in Banana Variety Name Country of Origin Genome- Sub group Fruit Ripening stage Total Carotenoid content Apantu Ghana AAB-Plantain Unripe Ripe 46.83 ppm 100.71 ppm Bira Papua New Guinea AAB-Pacific plantain Unripe Ripe 43.42ppm 106.38 ppm Pelipita Philippines ABB-Plantain Unripe Ripe 25.35 ppm 17.44 ppm To’o Papua New Guinea AA-Desert Unripe Ripe 5.60ppm 77.69ppm Source- Ekesa, 2015 Fortified Banana Normal Banana Biofortification in Fruit Crops
  • 26. Biofortification in pomegranate Solapur Lal- Hybrid Ganesh 5.6-6.1 mg/100g Iron 2.7-3.2 mg/100g 0.64-0.69 mg/100g Zinc 0.50-0.54 mg/100g 19.4-19.8 mg/100g Vitamin-C 14.2-14.6 mg/100g Solapur Lal- Hybrid  Fruit yield: 23.0-27.0 t/ha.  Developed by ICAR-National Research Centre on Pomegranate, Solapur.  Adaptation: Semi-arid regions of the country. Source- ICAR,2017 Biofortification in Fruit Crops
  • 27. NRCP H-6 Bhagwa 6.10 mg/100g Iron 2.8 mg/100g 0.69 mg/100g Zinc 0.52 mg/100g Biofortification in pomegranate A group of scientists from National Research Centre for Pomegranate(NRCP), Solapur, in collaboration with Indian Institute Horticulture Research (IIHR), Bengaluru, has developed a new hybrid pomegranate variety called "NRCP H-6", which has a high content of iron and zinc compared to the existing commercial variety Bhagwa. The news from- Times of India, 10th April 2017Biofortification in Fruit Crops
  • 28. Conclusion  Biofortification is a cost effective, feasible means of reaching populations who may have limited availability and access to diverse diets, supplements, or commercially fortified foods.  A one-time investment in developing for micronutrient rich varieties for farmers to grow for years to come, and the same varieties can be evaluated in other target geographies with similar agro-ecological conditions, thus multiplying the benefit of the initial investment.  Biofortification is one solution among many that are needed to solve the complex problem of micronutrient deficiency, and it complements existing interventions.  For biofortification it is vast scope to develop the biofortified varieties in fruits crops like; guava, annona spp., ber, papaya, jackfruit, pineapple, etc. Biofortification in Fruit Crops