1. Pandoravirus salinus is virus that infects amoeba, is very large ( 1 m ) and has a genome composed of double-stranded DNA that is 2.47 Mbp in size ( 247 , 000 , 000 bp). P . salinus has genes not typically associated with viruses, such as genes required for the synthesis of amino acids. In what ways might this force virologists to re-examine the definition of virus? 2. Many chemical biocides (disinfectants) target the lipid envelopes of viruses. Why would the chemical destruction of the envelope render the virus non-infectious? 3. Some viruses cause cancer. For example, Human Papillomavirus (HPV) that is associated with the development of cervical cancer and believed to play a role in head and neck tumors. Given what you know about viral replication, would the induction of cancer be beneficial to the virus? 4. What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)? .