Phytase in Poultry
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Use of exogenous phytase in poultry is briefly discussed
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Phytase in Poultry
- 1. Muhammad Arslan Musa arslan2062@gmail.com Muhammad Arslan Musa
- 2. PHYTATE • All Plant sources only contain 60-80% phytate phosphorus while only 20-40% phosphorus in free form (Non-phytate phosphorus) • Phytic Acid =Myo-inositol hexaphosphate (free form) • Phytin = complexes with calcium, magnesium and potassium • Phytate = Mixed salts of phytic acid (IP6) • Poultry birds have insufficient ability to degrade this phytate and utilize the phosphorus Muhammad Arslan Musa
- 4. POULTRY FEED USUALLY CONTAINS 2.5-4 G PHYTATE/ KG FEED Muhammad Arslan Musa
- 7. PHYTATE IS AN ANTI-NUTRITIONAL FACTOR • Binds divalent minerals and affect absorption i.e. P, Ca, Mg, Cu, Zn etc. • Enhance excretion of Na • Negatively affects energy metabolism • Interferes protein and carbohydrate metabolism • Increase indigenous amino acid loses Muhammad Arslan Musa
- 8. FACTORS AFFECTING PHYTATE UTILIZATION • Genetics • Age • Ca, P and vitamin D3 level in feed • Fiber contents of feed • Ingredient type • Phytase type Muhammad Arslan Musa
- 9. PHYTASE • Phytase activity firstly detected in rice bran (100 years ago) • Exogenous phytase development starts in 1962 • Commercially introduced in 1991 • Phytase hydrolysis phytate and alleviate its negative effects • Improve growth performance of birds • Commercial phytase prepared from recombinant Muhammad Arslan Musa
- 11. MOA OF PHYTASE Muhammad Arslan Musa
- 13. SOURCES OF PHYTASE • Plant phytase • Intestinal phytase • Microbial phytase • Fungus (Aspergillus niger, Aspergillus ficcum, Aspergillus oryzae) • Bacteria (Bacillus subtilis, Escherichia coli, Klebsiella aerogenes Corynebactrium bovis, Pseudomonas sps) • Yeast (Saccharomyces cerevisiae, Schwanniomyces castellii) Muhammad Arslan Musa
- 14. TYPES OF PHYTASE • 3-Phytase (i.e. A. niger) • 6-Phytase (i.e. E.coli, Peniophora lycii) Based on initiation of hydrolysis of phytate Muhammad Arslan Musa
- 16. P EQUIVALENCY OF MICROBIAL PHYTASE Muhammad Arslan Musa
- 17. PHYTASE ACTIVITY UNITS • FTU (fytase Units) • FYT • U (one unit of phytase) • PU Muhammad Arslan Musa
- 18. FTU One FTU is the amount of enzyme that liberates 1 µmol inorganic orthophosphate/min from 0.0051 mol/L sodium phytate at pH 5.5 and at temperature of 37◦C Muhammad Arslan Musa
- 19. U amount of enzyme that liberates 1 µmol inorganic P per minute from 0.0015 mol/L sodium phytate at pH 5.5 at 37oC Muhammad Arslan Musa
- 20. SITE OF PHYTASE ACTIVATION • Crop • Proventriculus • Gizzard Muhammad Arslan Musa