It provides basic knowledge regarding the formation of an egg, structure of egg, nutritional importance of eggs as well as egg processing technology and egg products.
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Introduction to Egg, Egg Products and
ProcessingTechnology
Muhammad Arslan
B.Sc. (Hons.) & M.Sc. (Hons.) Poultry Science (UAF)
arslan2062@gmail.com
Contents
Egg formation
Egg structure
Egg composition and food value
Egg Quality
Egg Processing
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Egg Formation Process in Chicken
Muhammad Arslan
B.Sc. (Hons.) & M.Sc. (Hons.) Poultry Science (UAF)
arslan2062@gmail.com
Chicken Egg
• Semi-solid nutritional mass enclosed in hard calcareous shell
produced by female of bird
• Chicken egg contain 8000-10000 pores in shell
• Hen requires 2-2.5 gram Ca for normal egg shell formation
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Egg Formation
Ovary = yolk formation
Oviduct = Albumin, shell membrane and shell
• Mature hen have 3600-4200 ova
• Egg formation requires 23-26 hours
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• Yolk matured in 10 day
• Ovulation take place 15-75 minutes after oviposition
• The earlobe color of hen can be an indication of eggshell color
with some exceptions
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Hormones during Egg Formation
FSH = Activate ovary and oviduct
LH = Release ova from ovary (Ovulation)
Progesterone = release LH from pituitary
Arginine Vasotocin = Uterine contraction (oviposition)
Testosterone= comb growth, albumin formation
Estrogen= plumage growth, mating and nesting behavior, oviduct
development, nutrient supply to ovary and oviduct
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ReproductiveTract
• Reproductive tract = ovary + oviduct
• In Chicken, only left side remains functional
• Right ovary and oviduct regressed during embryonic growth
• Mullerian Inhibiting Substance (MIS) is responsible for regression
• Right one is functional in Hawk while both sides functional in Brown Kiwi
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Ovary
• Smooth before laying and later looks granular
• Looks like cluster of grapes before laying
• Left ovary present near proximity to left kidney
• During incubation, approximately 20,000 ova present in hen
• Mature hen have 3600-4200 ova
• Ovary size is 40-50 g in laying hen and 3-5 grams in broody
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Medullary Bone Formation
• Soft and spongy bones inside long bones
• Calcium store for layer
• High estrogen level in blood initiate its formation
• Only present in females of chicken
• Highly important during night for egg formation in absence of feed
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Role of Light
• Photoreceptors are present in hypothalamus
• Photoreceptors perceive light and hypothalamus release GnRH
• GnRH act on pituitary to release FSH
• Specific light duration and intensity is required
• 16-17 hours light with 0.75 fc intensity
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Initiation of Egg Formation
• 11 days before first egg, anterior pituitary release FSH
• FSH activate ovary and oviduct
• Active ovary produced estrogen, progesterone and testosterone
• Hormonal + Nervous system is responsible for first ovulation
• Second ovulation regulated by first oviposition
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Estrogen
• Enlargement and redding of comb and wattles
• Medullary bone formation initiates
• Stimulate yolk formation in liver
• Widening of pubic bones
• Enable oviduct to secrete its secretions
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Release of Ova into the Oviduct
•When ova get matured, progesterone hormone is
released by ovary
•Progesterone stimulates hypothalamus to release
LH from anterior pituitary
•LH cause rupture of follicle from stigma (suture line)
and ovulation takes place
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Infundibulum (Funnel)
• Approx. 9 cm funnel-like structure
• Capture ova from ovary
• Sperms from males are temporarily stored here for fertilization
• Fertilization takes place if sperms are present
• Ova remains 15 minute here
• Only becomes active just after ovulation
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Egg Fertilization
• Cock mate 10-30 times a day with hen
• Semen ejaculation is 1 ml for first mating and then reduced to 0.5
ml or less
• 100 million to 5 billion sperms are ejaculated per mating
• Sperms are stored in utero-vaginal junction
• Fertile eggs can be produced for 4 weeks after removal of males
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• Movement of sperm from this junction to infundibulum
- 30 minutes in absence of ovulation
- 5-10 minutes after an ovulation
• 50 sperms penetrate outer layer of ovum (less than 30 leads to
poor fertility)
• Few sperms move to germinal disk
• 1 sperm unite with female pronucleus to form zygote
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Magnum
• Largest part of oviduct (33 cm long)
• Major portion of albumin secreted here
• Chalazae also secreted here
• All 4 layers of albumin produced and completed except outer thin white
• Developing remains here for 2-3 hours
• Only one type of albumin secreted in magnum but addition of water and
rotation of egg make layers
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Isthmus
•Small length of 10 cm
•Less developed than magnum
•Ova remains here for 75 minutes
•Inner and outer shell membranes secreted here
•Outer membrane is about 3 times thicker than inner
•Eggs from young birds have thicker shell membranes
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Uterus (Shell gland)
• 10-12 cm long in laying birds
• Egg remains here for 20-21 hours
• Fourth layer of albumin (outer thin layer) is completed here
by addition of water and salts
• Plumping
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• Approximately 15 ml fluid is added by plumping process
• Egg shell, its pigment and cuticle is added here
• 2-2.5 g calcium is used for shell formation from which 53% taken
from feed and 47 % from bones
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Vagina
• Last part of oviduct and12 cm long
• Egg remain for few minutes here
• No egg formation occurs here
• Only expel the eggs outside the body
• Sperm store glands are present at junction of shell gland &
vagina
• It can store sperms for 10-14 days
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Oviposition
• Egg laying process is called oviposition
•Arginine Vasotocin from anterior pituitary control shell
gland contraction during oviposition
• Broader end comes first in the environment
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• Initially egg has smaller end outward
• Just before oviposition hen rotates egg in horizontal position
and broader end comes first from body
• Rotation takes about 2 minutes
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Air Cell Formation
• Air cell (0.7 inches) formed between inner and outer shell
membrane at broader end
• Egg inside hen has no air cell
• When egg is laid its contents are contracted due to
temperature difference Muhammad Arslan
• As the egg contents contracted, air cell is formed
• Broader end comes first so normally it formed at broader end
• As egg get older, moisture contents evaporated from eggs, air
cell size increased
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Egg after Laying
•Freshly egg has temperature of about 104
•Egg takes 4-8 hours to attain room temperature
•Air cell formed after egg laying
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Cuticle
• Most external layer of egg
• Protect the egg from moisture and microbial invasion
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Eggshell
• 10-12 % of egg weight
• 7000-17000 funnel shaped pores
• Normal eggshells can withstand 3 to 4 kg (6 to 9 lb) per square
centimeter before breaking.
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FoodValue and Chemical Composition of
Chicken Egg
Muhammad Arslan
B.Sc. (Hons.) & M.Sc. (Hons.) Poultry Science (UAF)
arslan2062@gmail.com
Introduction
• Egg is an excellent food and well-balanced source of nutrients for all ages
• Provide good nutrition with comparatively low calorie contents
• High quality proteins with highest biological value
• Egg devoid fibers and its components are highly digestible
• Egg can contribute to overall health across the life span
• Eating two eggs per day covers 10% to 30% of the vitamin requirements for
humans
The perfect balance and diversity in its nutrients along with its high digestibility and its
affordable price has put the egg in the spotlight as a basic food for humans
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Egg proteins are distributed equally between egg white and egg yolk, while lipids, vitamins and minerals are essentially
concentrated in egg yolk
Egg White
Yolk
(Réhault-Godbert et al., 2019)
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Egg antimicrobials in edible parts are essentially concentrated in egg white and the vitelline
membrane
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Egg is an encapsulated source of macro and micronutrients that meet all
requirements to support embryonic development until hatching
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Egg-ceptions
• Natural source of vitamin D (Second to cod liver oil)
• Highly digestible and least-cost animal protein sources
• Multivitamin Source
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Health Benefits of Egg
• Rich nutrient source
• Muscle development (Proteins and amino acids)
• Eggs are second only to fish liver oils as a natural source of vitamin D
• Good for vision (Vitamin A, Lutein, Zeaxanthin)
• Good for cell functions, brain health and neurotransmission (Choline)
• Source of cholesterol (essential component of cell membranes and a precursor
for hormones, vitamin D, and bile acids)
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• Good for maintaining stronger bones (Vitamin D, Ca, P)
• Boost Immune system (Vitamin B12, E, Se, Zn)
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Egg Cooking and Nutrition
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Effect of Cooking on Egg Nutrients
(Réhault-Godbert et al., 2019)
Digestibility
Evenepoel P, Geypens B, Luypaerts A, Hiele M, Ghoos Y, Rutgeerts P. Digestibility of cooked and raw egg protein in humans as
assessed by stable isotope techniques. J Nutr. 1998 Oct;128(10):1716-22. doi: 10.1093/jn/128.10.1716. PMID: 9772141.
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Shell Membranes
• Two membranes surround the albumin
• The outer shell membrane is thicker and is attached to the shell
• Highly fibrous and permeable to moisture and gasses such as oxygen
and CO 2, but can prevent most microbial invasions
• Air cell formed at broader end
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Fresh eggs will sink in water while older eggs will
float
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Albumen
• 58 to 60% of the egg's weight
• Four layers
- Inner thick layer (Chalaziferous layer) 3 %
- Outer thick layer 57%
- Inner thin layer 17 %
- Outer thin layer 23 %
• Large amount of ovomucin is present in inner thick and almost absent in
outer thin
• The percentage of thin albumen increases as hens age and as eggs age
• Chalazae hold the yolk in the center
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Yolk
• Constitutes 30 to 32% of the egg's weight
• Surrounded by vitelline membrane
• Concentric rings of white and yellow yolk material due to alternating
deposition during day and night
• Fat-soluble pigments, normally xanthophyll, are transferred rapidly from the
digestive tract to the blood stream and then to the yolk
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• The deep yellow yolk is formed in the daytime, and the light yellow
yolk is formed at night
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Egg Quality Evaluation and
Grading
Muhammad Arslan
Muhammad Arslan
B.Sc. (Hons.) & M.Sc. (Hons.) Poultry Science (UAF)
arslan2062@gmail.com
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Muhammad Arslan
Purposes of Egg Quality Evaluation
• To evaluate the status of farm
• To benchmark the quality of eggs against the offering of competitors
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Egg Structure
Egg Size
1. Egg shape (Oval, become slightly elongated over the cycle)
2. Egg weight (Genetic, age, nutrition)
• With an extra 1g of protein consumed per day translating into an average
increase of 1.4g in egg weight
• Egg weight is used in many countries to grade and sell the eggs
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Egg Shell Quality
• Appearance and cleanliness (color uniformity, clean, smooth etc.)
• Shell thickness (0.33-0.37)
• Eggshell breaking strength (above 3.5 kgf/ 34 N)
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Egg Freshness
• Albumin Quality
• Yolk
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Albumin Quality
• Albumen contains a large amount of carbon dioxide
• Its pH ranges from 5.6 to 7.5 with time and gas exchanges with the outside
environment
• When the carbon dioxide dissipates, the pH of the albumen rises to about 9.5
• This rise in pH will affect the structure of ovomucin
• It make the albumen lose its viscosity
• Haugh unit (HU) is the common method for determining albumin quality
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Haugh Unit
HU= 100log(h-1.7w0.37+7.6)
h=albumin height (mm)
W= egg weight (grams)
The Haugh unit value ranges from 0 - 130 and can be ranked as below:
• AA : 72 or more
• A : 71 - 60
• B : 59 - 31
• C : 30 or less
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Egg Grading
Grading
• Grading is the sorting of products according to quality, size, weight, and other
factors that determine the relative value of the product
• Egg grading is the grouping of eggs into lots having similar characteristics as to
quality and weight
• U.S. standards for quality of individual eggs have been developed
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Advantages of Grading
• Sorted eggs bring more money to the investor than unsorted
• Reduces wastage
• Eliminates the need for personal inspection of the eggs by sellers, buyers, and
other interested people
• Development of improved quality at producer level through "buying on
grade" programs
• Market price reporting in terms understood by all interested parties
• A basis for settling disputes involving quality
• A basis for paying damage claims
• A basis for developing advertising
• A uniform basis for establishing brand names
• Establishment of buying guides for consumers
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Quality Factors
• Quality may be defined as the inherent properties of a product that
determine its degree of excellence
• Those conditions and characteristics that consumers want, and are willing
to pay for, are, in a broad sense, factors of quality
• Quality factors are classified as External and Internal
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Shell Shape andTexture
• The normal egg has an oval shape with one end larger than the other, and it
tapers toward the smaller end
• The ends of an egg are commonly called the large end (air cell end) and the
small end
• Strength and appearance of many eggs to develop the "ideal" egg shape
• The shape of an egg can be considerably different from the ideal, but may still
be considered practically normal
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• Eggs that are unusual in shape may have ridges, rough areas, or thin spots
• Abnormal shells may result from improper nutrition, disease, or the physical
condition of the hen
• Sometimes a shell is cracked while the egg is still in the body of the hen. These
eggs, which are commonly referred to as "body checks," are repaired by an
additional deposit of shell over the cracked area, generally resulting in a ridged
area.
• Shells with thin areas and some other types of defects are usually weaker than
normal shells, and the danger of breakage en route to the consumer lowers the
utility value of the egg. Eggs of abnormal shape also lack consumer appeal.
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Interior Egg Quality
• Even under the most favorable conditions, egg quality is relatively unstable as
the interior quality of the egg deteriorates
• Sometimes quality changes render eggs useless for food before they reach
consumers
• However, when eggs are properly cared for, the quality decline can be minimized
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Air Cell
• Temperature of freshly laid egg is about 105 ⁰F
• As the egg cools, the liquids contract more than the shell
• The inner shell membrane separates from the outer to form an air space
• Evaporation of water from the egg increase air cell size
• The air cell is the easiest quality factor to evaluate, as it can be judged
objectively by a simple measuring device “Air cell gauge”
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The interior quality of broken-out eggs for each of the
designated grades as specified in the U.S. standards with
respect to white and yolk quality
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Egg Processing and Products
Muhammad Arslan
M.Sc. (Hons.) Poultry Science
arslan2062@gmail.com
Muhammad Arslan
Introduction
• Shell eggs are transported to egg processing plant
• “Egg products” refers to eggs that are removed from their shells for processing
• Egg products processing includes breaking eggs, filtering, mixing, stabilizing,
blending, pasteurizing, cooling, freezing or drying, and packaging
• Egg products include whole eggs, whites, yolks, and various processed and
pasteurized blends, with or without non-egg ingredients
• Egg products may be available in liquid, frozen, and dried forms
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Egg Processing in Pakistan
• Al Waha is a pioneer in processing egg products
• Barkat Frisian Pasteurized Egg Company (Pvt) Ltd is a Pakistani – Dutch
Joint Venture Company
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Egg Processing
• Receiving shell eggs was
• Washing/sanitizing/candling eggs
• Breaking eggs
• Handling liquid egg product: – filtering – blending – mixing
• Cooling
• Pasteurization
• Freezing or drying
• Packaging
• Storage
• Shipping
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Receiving of Shell Eggs
• In-line system vs off-line system
• Production starts with loading of eggs
• Vacuum cups transfer the eggs from the flats to the egg washer conveyor (Off-
line)
• Off-line operations usually have separate coolers for incoming eggs for overnight
cooling
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Cleaning and Sanitization
• Primary objectives are to remove surface contaminants, to enhance appearance and
appeal of the egg to the consumer, and to assure a safe high quality product
• Shell eggs, when presented for breaking, must be clean
• Washing is followed by sanitizing and then by immediate drying
• Modern egg washers use pressure sprays, rotating brushes, and an egg-spinning device
• Increases contact between the egg and the brush and minimizes damage to the eggs
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• Sodium hypochlorite (NaCIO) is the most commonly used sanitizer
• Shell eggs ineligible for breaking during the candling operation
• After washing and sanitizing, eggs are air dried with the use of
electrical fans, and oiled to help reduce weight loss and preserve
interior egg quality
• Shell eggs continue on the conveyor to the breaking room
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WashWater
• Wash water temperature must be at least 90°F (32.2°C) to provide appropriate cleaning
• Further, wash water should be at least 20°F (11°C) warmer than egg temperature to
help prevent microbial penetration
• In practice, a range of 100 to 114 °F (37.8 to 46.1°C) is commonly used
• The initial pH of alkaline detergent wash water should be approximately 11
• During the washing cycle, water pH should range between 10 and 11
• Salmonella are able to grow at temperatures of 100 to 108°F (38 to 42°C) when wash
water pH is 9.5 or less
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Egg Candling
• Eggs are mechanically rotated several times over a bright light to examine the
internal quality of the egg
• Critical step in the processing of the shell eggs
• Defective eggs are identified and removed
• Inedibles are eggs with broken shells and membranes (leakers),
dirty, or eggs with blood spots
• Plant employees sort, classify, and segregate the eggs to ensure that only eggs
eligible for breaking enter the breaking room
Defective Eggs
Sent for breaking
Destroyed, if inedible
Rewashed
Shell Eggs Packing
• From the grading area, eggs are conveyed to the packaging machine, where they
are individually weighed and separated into one of six weight
• Containers are commonly stamped with date-of-packing and sell-by-date codes
to facilitate inventory control
• In the United States, shell eggs were commonly refrigerated at 55 to 66°F (12.6 to
15.6°C) during pre- and post-processing and in retail storage and displays
• In most parts of the world, eggs are not refrigerated
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Specific Flock Management Systems
Free-range
Barn
Vegetarian-diet eggs
Organic shell eggs
Designer eggs (modification/fortification)
Modifications
Cholesterol reduction
Elevating vitamin E level
Changes in fatty acid
profile
Fortifications
Nutraceuticals
supplementation
Omega-3 fatty acids
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Further Processed Egg Products
• These are out-of-the-shell egg ingredients which are combined with other
ingredients to create finished food products for retail and foodservice users
• Further processed egg products are classified into four groups:
i. Refrigerated liquid egg products
ii. Frozen egg products
iii. Dried egg products
iv. Non food by-products
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Breaking Eggs
• This process transforms shell eggs into liquid products: whole egg,
egg whites and egg yolks
• After the egg is broken, the breaking machine can separate the yolk
from the white
• Further-processed liquid eggs are then manufactured into chilled,
frozen, or dried products
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Handling of Liquid Eggs
• After breaking, the liquid egg flows into collection vats (balance tanks) by gravity
• A perforated plate contained within the vat serves as a filter to remove eggshells
• The liquid egg is pumped through a filter and then is pumped to the cooling
system
• After cooling, the liquid egg is pumped to a storage silo for
further processing
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• Pasteurization, stabilization, or drying operations shall start as soon as
practicable after breaking to prevent deterioration of product
• Preferably within 72 hours from time of breaking for egg
• Liquid products can be used as is, or can be incorporated as an
ingredient into other finished food products
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Pasteurization
• Liquid egg products are pasteurized to eliminate Salmonella
• Pasteurization involves rapidly heating the product and holding the product at a
minimum required temperature for a specific time
• Rapid cooling of liquid eggs during pre- and post-pasteurization further improves
product quality, safety, and shelf life
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Pasteurized liquid egg product may be handled as
i. Packed in containers (plastic buckets, bag-in-box, packages, milk
containers, etc.) to be marketed
ii. Further processed (add ingredients post pasteurization, freeze or dry)
iii. Shipped in tankers to another egg products plant or distributor
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Why Pre and Post Pasteurization Refrigeration is Required?
• Many spoilage bacteria and spores remain viable after pasteurization
• Without refrigeration, these organisms will multiply rapidly, to about 10 million
cells per gram, at which time the egg product starts to deteriorate
• Refrigeration slows down this process and increases the shelf life of the product
• Furthermore, microorganisms release enzymes into the media in which they live
Some of these enzymes are heat-resistant, thus they will survive pasteurization
• Rapid breaking + pasteurization + pre/post refrigeration = reduce enzyme activity
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USDA Regulations for Pasteurization
• All egg products be free of Salmonella, which is accomplished through the use of
pasteurization
• The USDA requires that all pre-pasteurized liquid egg products be refrigerated to
holding temperatures between 40 and 70 ⁰ F (4.4 and 21.1°C)
• The specified temperatures must be achieved within 2 hours from the time the
eggs are broken
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Temperature, time, and pH all affect the successful pasteurization of
liquid eggs
Higher pH requires
lower
pasteurization
temperature with
the alkaline pH of
9.0 being the most
effective for
destroying
Salmonella
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Storage and Distribution of Liquid Egg Products
• Chilled liquid egg products are extremely perishable and should be
rapidly cooled immediately after pasteurization to 40°F (4.4°C) or
below, and kept cold at 34 to 40°F (1.1 to 4.4°C) during storage
• Shelf-life at 34 to 40°F (1.1 to l.4°C) is about 5 to 12 days, depending
on the product's quality
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Freezing
• Frozen egg products include separated whites and yolks, whole eggs, blends of
whole eggs and yolks, and whole eggs with added ingredients
• They are produced from pasteurized liquid egg products by using a blast freezer
at temperatures of –10°F to – 40°F
• Frozen egg products have a long shelf life when kept at less than 10°F
• Freezing whole liquid eggs or yolks to below 21°F (-6°C) creates gelation problems
and when these eggs are thawed, they do not have the same characteristics as
shell eggs or fresh egg products
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Gelation
• Gelation is an irreversible increase in the apparent viscosity
• The thawed yolks do not return to their original smooth texture, but become lumpy,
gummy, and do not mix well with other ingredients
• Gelation is formed when water molecules freeze around the egg's lipoproteins
• Gelation of liquid whole egg is less severe than that of yolk, as egg albumen is only
slightly affected
• To prevent gelation, salt or sugars are added to the yolk at a 10% level
• Mechanical action, such as passage through a homogenizer, also prevents gelation
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Packaging and Storage of Frozen Products
• The frozen products are stored in a cold room with ambient temperature of
0 to –l0 °F (-17.8 to -23.3°C)
• To prevent gelation, salted yolks are frozen and stored at 0 °F (-17.8°C)
• One of the major drawbacks of frozen egg products is the lengthy thaw
time which reduces the flexibility of work scheduling, especially in
emergencies or when additional eggs are needed
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Drying (Dehydration of Eggs)
• Industry has developed a variety of dried egg products, including dried egg white,
dried plain whole egg and yolk, and specialty dried egg products
• Egg products fall under two basic categories when considering their drying
characteristics
i. egg white products
ii. whole egg and yolk products
• Before the liquid egg products go through the drying process, industry uses
processing techniques to achieve the desired finished product
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• Industry uses chemical additives to improve and keep the functional
properties (whipping, coagulation, emulsification, flavor, nutrition,
and color) of the final dried egg products
• Examples of chemical additives include carbohydrates, whipping aids,
emulsifiers, and anti-caking agents (colloidal silicon dioxide)
• The dried egg product must be handled aseptically during packaging
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Drying Systems
1. Spray Drying
2. Pan Drying
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Spray Drying
• The liquid egg is atomized by pressure nozzles which operate at 500 to 6,000 psi
• The centrifugal atomizer consists of a rotating or spinning disc or rod, which
rotates at 3,500 to 50,000 rpm
• The atomized droplets meet a hot air cyclone at 250 to 450°F (121 to 232°C),
which is created and driven by a fan blowing it in the opposite direction
• The moisture level in spray dehydrated products is approximately 5%
Types of Spry Dryers
Vertical (Most widely used) Horizontal
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Desugarization of Liquid Egg Products Prior to
Drying
• Millard Reaction (reducing sugar + proteins)
• As a result, the color of the products can be darkened from yellow to brown and
the products shelf life can be significantly reduced
• Therefore, the glucose in the eggs must be removed before drying
• The glucose fermentation process is widely
used today by most commercial operations
Desugarization Methods
Bacterial fermentation
Yeast fermentation
Glucose oxidase-catalase enzyme fermentation
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Freeze-Drying Eggs (lyophilization)
• Water is removed from food by direct sublimation
• If the frozen food is placed under a high vacuum and heated, the moisture in the
product will be transformed into vapor without first being liquefied
• Freeze-dried eggs are superior to air-dried eggs but are also much more
expensive (up to four times) to produce
Muhammad Arslan
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Packaging of Dried Egg Products
• Fiber drums with polyethylene liners for packaging dried egg products
• Thick paper bags with liners for 25 or 50 lb (11.4 or 22.8 kg) of product are
also used in other countries
• Liners are required to protect the egg products from absorbing moisture
through the packaging walls
• Freeze-dried eggs are more hygroscopic, and therefore require packaging
with improved moisture barriers
Muhammad Arslan
Storage of Dried Egg Products
• Dry glucose-free egg whites are very stable under most storage
conditions with years of shelf life
• Yolk-containing products are less stable and have about 8 months
shelf life in dry storage at room temperature
• Store successfully for more than a year in refrigeration
Muhammad Arslan