Advance Genetics 101

1. GENETIC ENGINEERING
AND APPLICATIONS
Louie Jane T. Eleccion

2. • Define Genetic Engineering.
• Outline the process of genetic engineering involving:
isolation, cutting, transformation, introduction of
base sequence changes and expression.
• Site applications/uses of Genetic Engineering in
different fields (Agriculture and Medicine).
OBJECTIVES

3. Important Terms
• Plasmid – is a circular bacterial genome which consists a
single DNA, containing non-essential genes but carry
genetic advantages.
• Restriction Enzyme – molecular scissors that cuts DNA
into fragments.
• Recombinant DNA – the term used to describe the
combination of two DNA strands that are constructed
artificially.

4. GENETIC ENGINEERING
The direct
manipulation of
an organism's genome
using biotechnology.

5. Biotechnology
• Is any technological application that makes use of
biological systems, living organisms and its
components to create products and other
technological systems with the aim of advancing the
human condition.

6. GENETIC ENGINEERING
• It is the process by which it artificially copies a piece of
DNA from one organism and joining this copy of DNA
into the DNA of another organism.

7. Purpose of Genetic Engineering
It allows genes from one organism to be inserted into a cell
of a different organism of a different species.

8. Genetic Engineering Processes
OVERVIEW
• Alteration in the segment of a DNA molecule (rDNA).
• Recombinant DNA is joined to other unrelated DNA
in the organism.
• This is called gene splicing.
- tiny segments of a gene are taken out and
replaced by different genes

9. TRANSGENIC ORGANISMS
• Organisms altered by genetic engineering through
gene transfer—
the movement or insertion of a gene into an
organism that normally does not have a copy
of that gene.
• The organism receives new gene from the other
organism.

10. Other Examples of Transgenic
Organisms
• GMO – genetically modified organism
• GEO – genetically engineered organism
For example
– Plants that resists a particular type of weed
killer
– Sheep which makes some special substance in
its milk.

11. Genetic Engineering Process

12. Stages involved in GE
1. Isolation
2. Cutting
3. Transformation
4. Ligation and Insertion
5. Expression

13. 1. ISOLATION
(a) Isolation of a specific gene from donor e.g. human
• Cells broken open
• Genetic probe added
Donor DNA
Genetic probe
Position of
gene of
interest

14. Bacterial cell
Plasmi
d
(b) Isolation of plasmid from a bacterial cell
www.sci.sdsu.edu
1. ISOLATION

15. 2. CUTTING
• Restriction enzymes cut DNA at
specific sites called restriction
sites. Restriction site
Restriction
site Restriction
ezymes

16. 2. CUTTING

17. 17
Restriction site Restriction site
Donor DNA
© Biology Support Service 2007
2. CUTTING
Plasmid
Restriction
enzymes

18. 2. CUTTING
Sticky EndsPlasmid
Donor DNA

19. DNA Ligase
http://www.slic2.wsu.edu:82/hurlbert/micro101/pages/Chap10.html#Sticky_ended_cut
Ligation –rejoining cut fragments of DNA and
forming artificial recombinant molecules

20. 3. LIGATION AND INSERTION

21. 4. TRANSFORMATION
Recombinant DNA introduced into bacterial cell.
Bacterial
chromosome
Bacterial
cell
Recombinant DNA

22. 5. EXPRESSION
• Bacterial cell reproduces by Binary Fisson
• Bacterial cell produces the polypeptide
coded for by the donor DNA

23. • Expression is getting the organism with the
recombinant DNA to produce the desired protein
• When the protein is produced in large amounts it is
isolated and purified
5. EXPRESSION

24. Summary of Steps
1. Cut with restriction
enzymes
Donor DNA
Plasmid
Donor DNA
Sticky
Ends
2. Ligase bonds
sticky ends
together
Recombinant
DNA

25. GE in Plants

26. GE in Animals (Cloning)

27. Animals used in GE
• The human gene to clot blood has been inserted into
the DNA of sheep
• Sheep produce human clotting factor needed for
Haemophiliacs in their milk.
• Goats produce a protein to treat emphysema

28. Genetic Engineering
APPLICATIONS

29. AGRICULTURE
MEDICINE

30. AGRICULTURE
- PRODUCTIVE
- LESS FERTILIZER
- DISEASE
RESISTANT

31. IMPROVE SEVERAL
AGRICULTURAL CROPS
• TRANSFER OF NITROGEN FIXING GENES
• TRANSFER OF RESISTANCE AGAINST PATHOGENS
• IMPROVEMENT IN QUALITY AND QUANTITY OF
SEED PROTEINS
• TRANSFER OF GENES FOR ANIMAL PROTEINSTO
CROP PLANTS

32. - GENETIC DISEASES BECOME PREVALENT
- VACCINES
- HORMONES
- GENETHERAPY
- GENE PHARMING
MEDICINE

33. • VACCINES
- to form protective antibodies
-The use of genetically
modified yeast cells to
produce a vaccine against
the hepatitis B virus has
been a major success story.
MEDICINE

34. • HORMONES
- Bacterial cells can be factories to
produce
human insulin
MEDICINE

37. • GENETHERAPY
- It involves modifying
human DNA either to repair
it or to replace a faulty gene.
- Cystic fibrosis is the best
known disease
MEDICINE

38. - GENE PHARMING
- first mammal engineered (1990)
- Ian Wilmut at RoslinInstitute at
Scotland
- Zygote genetically engr’d through DNA
injection
- produce milk (containing large amount
of human enzyme alpha-1 antitrypsin
- cystic fibrosis
- emphysema TRACY THE SHEEP
MEDICINE

39. Thank you 