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© 2016 GENXPLORE
Exploring the spiral of life…….
Dr. D.Shanmuganandham, BDS,
MSc(F.Odont)
Research Associate
© 2016 GENXPLORE
• PC-PNDT 1994 Act
• NABL ID “M-1255”
• DSIR – Applied
Recognition
© 2016 GENXPLORE
“An insight into Dental Evidence
as an Expert’s Witness”
© 2016 GENXPLORE
Objective
• The ability to identify types of dental evidence. This includes the various
transfer surfaces and materials that may capture dental evidence.
• Appreciate the forensic identification significance and limitations of these
categories of dental evidence.
• Properly document, collect and preserve these categories of dental physical
and biological evidence.
• Knowledge of dental materials and supplies associated with evidence
collection and preservation.
© 2016 GENXPLORE
Evidence
Evidence, broadly construed, is anything presented in
support of an assertion.[1] This support may be strong or
weak. The strongest type of evidence is that which provides
direct proof of the truth of an assertion.
© 2016 GENXPLORE
Types of Evidence[2]
• Direct evidence
• Circumstantial evidence
• Anecdotal evidence
• Character evidence
• Demonstrative evidence
• Digital/Electronic evidence
• Documentary evidence
• Exculpatory evidence
• Forensic evidence
• Hearsay evidence
• Physical evidence
• Prima facie evidence
• Statistical evidence
• Testimonial evidence
• Judicial evidence
• Non-judicial evidence
• Primary evidence
• Secondary evidence
• Real evidence
• Oral evidence
• Scientific/Expert/Trace Evidence
© 2016 GENXPLORE
Dental Evidence
© 2016 GENXPLORE
What is dental evidence?
• The gamut of evidence that is either directly related to human dental
anatomy or derived from the oral environment.
© 2016 GENXPLORE
Primary Identifiers
9
© 2016 GENXPLORE
Dentist as a witness in Legal Case
• Accident(RTA)
• Juveniles cases (Child marriage)
• Sexual assault
• Physical violence
• Abuse & neglect
• Homicide cases
• Human trafficking victim recognition
• Dental negligence/ malpractice
• Insurance (Fraudulent claim)
10
© 2016 GENXPLORE
Cont….
• Child labor case
• Disaster (natural, manmade)
• Skeletal remains (Missing or unidentified Human remains)
• Sports
• Regional transport office(RTO)
• Dental record management
11
© 2016 GENXPLORE
Role of Dental Evidence in legal case
• Age estimation
• Sex determination
• Disaster victim identification(Comparative human identification)
• Pattern injury identification (bite mark, domestic violence)
• Race determination
• Species determination
• Dental Record (Ante mortem & postmortem record)
• Dental profiling/charting(insurance)
• Dental neglect and malpractice
• Facial reconstruction
• Lip print comparison
12
© 2016 GENXPLORE
Broadly classified into:
I. Visual
II. Radiographic
III. Chemical
IV. Histological
13
Age Estimation
© 2016 GENXPLORE
Average Stage of Attrition
14
REFERENCE: Li, C., & Ji, G. (1995). Age estimation from the permanent molar in northeast China by the method of average stage of
attrition. Forensic Science International, 75(2-3), 189–196. doi:10.1016/0379-0738(95)01791-7
Visual method
© 2016 GENXPLORE
Radiological method
• Schour and Masseler method (1941)
• Nolla’s method (1960)
• Moorees, Fanning and Hunt method (1963)
• Demirjian & Willem method
• Coronal Pulp Cavity Index
• Kvaal et al
REFERENCE: Dental radiographic indicators, a key to age estimation
AS Panchbhai Dentomaxillofacial Radiology 2011 40:4, 199-212
© 2016 GENXPLORE
Chemical Method
Amino acid racemisation
© 2016 GENXPLORE
Histological method
-Incremental lines of cementum
© 2016 GENXPLORE
Translucency method
Root Dentin Translucency
Conventional Calliper Method
Bang, G., & Ramm, E. (1970). Determination of Age in Humans from
Root Dentin Transparency. Acta Odontologica Scandinavica, 28(1), 3–
35. doi:10.3109/00016357009033130
© 2016 GENXPLORE
SEX DETERMINATION
• Morphological method
• Molecular method
• Radiological method
• Microscopic method
19
© 2016 GENXPLORE
Morphological method
REFERENCE: Gupta B, Gupta M. Sex identification in forensic odontology- a review of various methodology. Int J Forensic Odontol 2016;1:9-
13.
20
© 2016 GENXPLORE
MANDIBULAR CANINE INDEX
•MCI in identification of sex has never exceeded 87.5%it can
only be used as asupplemental tool.
REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology:
A review. J PharmBioall Sci 2015;7:S398-402.
21
© 2016 GENXPLORE
SEX DETERMINATION BY ROOT LENGTH AND
CROWN DIAMETER
• -80%accuracy
REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J
PharmBioall Sci 2015;7:S398-402. 22
© 2016 GENXPLORE
REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in
forensic odontology: A review. J PharmBioall Sci 2015;7:S398-402. 23
© 2016 GENXPLORE
REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology:
A review. J PharmBioall Sci 2015;7:S398-402. 24
© 2016 GENXPLORE
Radiological method
25
© 2016 GENXPLORE
Gonial Angle Measurements as Predictors of Sex
REFERENCE: Taleb NSA, Beshlawy ME (2015) Mandibular Ramus and Gonial Angle Measurements as Predictors of Sex and Age in an
Egyptian Population Sample: A Digital Panoramic Study. J Forensic Res 6: 308. doi: 10.4172/2157-7145.1000308
26
© 2016 GENXPLORE
Mandibular ramus: An indicator for sex determination
REFERENCE: Saini V, Srivastava R, Rai RK, Shamal SN, Singh TB, Tripathi SK. Mandibular ramus: An indicator for sex in
fragmentary mandible.J Forensic Sci 2011;56(Suppl1):S13-6.
27
© 2016 GENXPLORE
Microscopic methods
Barr bodies
• Presence or absence of X chromosome can be studied from buccal smears,
skin biopsy, blood, cartilage, hair root sheath, and tooth pulp
REFERENCE: Barr ML, Bertram LF, Lindsay HA. The morphology of the nerve cell nucleus, according to sex. Anat Rec
1950;107:283-97
28
© 2016 GENXPLORE
Molecular method
29
© 2016 GENXPLORE
Sex determination using Barr bodies
30
© 2016 GENXPLORE
Molecular method from Pulpal tissue
REFERENCE:Das N, Gorea RK, Gargi J, Singh JR. Sex determination from pulpal tissue. J Indian Acad Forensic Med 2004;26:50-4.
31
© 2016 GENXPLORE
Sex determination using polymerase chain reaction
REFERENCE: Tsuchimochi T, Iwasa M, Maeno Y, Koyama H, Inoue H, Isobe I, et al. Chelating resin-based extraction of DNA from dental pulp
and sex determination from incinerated teeth with Y-chromosomal alphoid repeat and short tandem repeats. Am J Forensic Med Pathol
2002;23:268-71.
32
© 2016 GENXPLORE
BITE MARK ANALYSIS
•Scaled photography of bite mark evidence
•Scaled photography of dental casts
•Clinical comparisons
•Computer comparisons
REFERENCE: MacDonald DG. Bite mark recognition and interpretation. J Forensic Sci Soc. 1974 Jul; 14(3): 229 33
33
© 2016 GENXPLORE
34
© 2016 GENXPLORE
Double Swabbing Technique
© 2016 GENXPLORE
© 2016 GENXPLORE
© 2016 GENXPLORE
Teeth Impressions
REFERENCE: MacDonald DG. Bite mark recognition and interpretation. J Forensic Sci Soc. 1974 Jul; 14(3): 229 33
38
© 2016 GENXPLORE
REFERENCE: Naru AS, Dykes E . The use of a digital imaging technique to aid bite mark analysis. Sci Justice. 1996 JanMar; 36(1): 4750
39
© 2016 GENXPLORE
REFERENCESweet D, Bowers CM. Accuracy of bite mark overlays: a comparison of
five common methods to produce exemplars from a suspect’s dentition. J Forensic40
© 2016 GENXPLORE
REFERENCE: Webster G. A suggested classification of bite marks in foodstuffs in forensic dental analysis. Forensic SciInt. 1982 JulAug; 20(1):
4552.
41
© 2016 GENXPLORE
42
© 2016 GENXPLORE
Race determination
REFERENCE: Edgar HJH. Prediction of race using characteristics of dental morphology. J Forensic Sci. 2005; 50(2): 269–73.
REFERENCE: Lasker GW LM. Racial traits in human teeth. J Forensic Sci. 1957; 2: 401–149.
43
© 2016 GENXPLORE
Species determination
REFERENCE: B. Rai, J. Kaur. Evidence-Based Forensic Dentistry. Springer-Verlag Berlin Heidelberg; 2013. DNA technology and Forensic
Odontology.
REFERENCE: Handbook of Forensic Medicine. First Edition. John Wiley & Sons, Ltd; 2014.
44
© 2016 GENXPLORE
RUGOSCOPY
REFERENCE: Thomas CJ, Kotze TJ. The palatal rugae pattern: A new classification. J Dent. Assoc. S. Afr. 1983;38:153–157.
45
© 2016 GENXPLORE
REFERENCE: Ohtani M, Nishida N, Chiba T, Fukuda M, et al. Indication and limitations of using palatal rugae for personal identification in
edentulous cases.
REFERENCE: M Virdi, Y Singh, A Kumar. Role of Palatal Rugae in Forensic Identification of the Pediatric Population. The Internet Journal of
Forensic Science. 2009 Volume 4 Number 2
46
© 2016 GENXPLORE
CHEILOSCOPY
REFERENCE: Tsuchihashi Y. Studies on personal identification by means of lip prints. Forensic Sci. 1974 Jun;3(3):233–248.
47
© 2016 GENXPLORE
Dental Record
REFERENCE: American Academy of Pediatric Dentistry. Guideline on Record keeping. Pediatr Dent. 2004; 34(special issue): 132-136.
48
DENTURE MARKING
REFERENCE :Colvenkar SS. Lenticular card: A new method for denture identifi cation. Indian J Dent Res 2010;21:112-4.
© 2016 GENXPLORE
49
© 2016 GENXPLORE
50
© 2016 GENXPLORE
51
© 2016 GENXPLORE
52
© 2016 GENXPLORE
53
© 2016 GENXPLORE
COMPARISON OF DENTAL RECORDS AND ANOMALIES IN
THE TEETH
REFERENCE: Krishan, K., Kanchan, T., & Garg, A. K. (2015). Dental Evidence in Forensic Identification – An Overview, Methodology and
Present Status. The Open Dentistry Journal, 9, 250–256. http://doi.org/10.2174/1874210601509010250
54
© 2016 GENXPLORE
REFERENCE: Krishan, K., Kanchan, T., & Garg, A. K. (2015). Dental Evidence in Forensic
Identification – An Overview, Methodology and Present Status. The Open Dentistry Journal, 9,
250–256. http://doi.org/10.2174/1874210601509010250
55
© 2016 GENXPLORE
Document injuries
56
© 2016 GENXPLORE
Identification of the victims
•In natural and man made disaster, acts of violence
may it be traffic accidents or human right
violation
57
© 2016 GENXPLORE
Identification in Mass Disaster
Tsunami
Flood
Earthquake
58
© 2016 GENXPLORE
Accidental Disasters
Flight accident
Train accident
Road traffic
accident
59
© 2016 GENXPLORE
INTENTIONAL MASS DISASTERS
Bomb blast World trade center attack in 2001
60
© 2016 GENXPLORE
Role of the FO in identification of victims
SEARCH &
RECOVERY
PM EXAMINATION AM DATA COLLECTION
RECONCILIATION DEBRIEFING
61
© 2016 GENXPLORE
References
1. Davis Oldham: 'Evidence' (English 101 & 102) at Shoreline Community College, shoreline.edu
“https://app.shoreline.edu/doldham/SRR.html” Accessed 10th may 2020
2. https://blog.ipleaders.in/different-kinds-of-evidence/ Accessed 10th may 2020
© 2016 GENXPLORE
Thank You……
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youtube.com/legalsaviour
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An insight into dental evidence as experts witness

  • 1. © 2016 GENXPLORE Exploring the spiral of life……. Dr. D.Shanmuganandham, BDS, MSc(F.Odont) Research Associate
  • 2. © 2016 GENXPLORE • PC-PNDT 1994 Act • NABL ID “M-1255” • DSIR – Applied Recognition
  • 3. © 2016 GENXPLORE “An insight into Dental Evidence as an Expert’s Witness”
  • 4. © 2016 GENXPLORE Objective • The ability to identify types of dental evidence. This includes the various transfer surfaces and materials that may capture dental evidence. • Appreciate the forensic identification significance and limitations of these categories of dental evidence. • Properly document, collect and preserve these categories of dental physical and biological evidence. • Knowledge of dental materials and supplies associated with evidence collection and preservation.
  • 5. © 2016 GENXPLORE Evidence Evidence, broadly construed, is anything presented in support of an assertion.[1] This support may be strong or weak. The strongest type of evidence is that which provides direct proof of the truth of an assertion.
  • 6. © 2016 GENXPLORE Types of Evidence[2] • Direct evidence • Circumstantial evidence • Anecdotal evidence • Character evidence • Demonstrative evidence • Digital/Electronic evidence • Documentary evidence • Exculpatory evidence • Forensic evidence • Hearsay evidence • Physical evidence • Prima facie evidence • Statistical evidence • Testimonial evidence • Judicial evidence • Non-judicial evidence • Primary evidence • Secondary evidence • Real evidence • Oral evidence • Scientific/Expert/Trace Evidence
  • 8. © 2016 GENXPLORE What is dental evidence? • The gamut of evidence that is either directly related to human dental anatomy or derived from the oral environment.
  • 10. © 2016 GENXPLORE Dentist as a witness in Legal Case • Accident(RTA) • Juveniles cases (Child marriage) • Sexual assault • Physical violence • Abuse & neglect • Homicide cases • Human trafficking victim recognition • Dental negligence/ malpractice • Insurance (Fraudulent claim) 10
  • 11. © 2016 GENXPLORE Cont…. • Child labor case • Disaster (natural, manmade) • Skeletal remains (Missing or unidentified Human remains) • Sports • Regional transport office(RTO) • Dental record management 11
  • 12. © 2016 GENXPLORE Role of Dental Evidence in legal case • Age estimation • Sex determination • Disaster victim identification(Comparative human identification) • Pattern injury identification (bite mark, domestic violence) • Race determination • Species determination • Dental Record (Ante mortem & postmortem record) • Dental profiling/charting(insurance) • Dental neglect and malpractice • Facial reconstruction • Lip print comparison 12
  • 13. © 2016 GENXPLORE Broadly classified into: I. Visual II. Radiographic III. Chemical IV. Histological 13 Age Estimation
  • 14. © 2016 GENXPLORE Average Stage of Attrition 14 REFERENCE: Li, C., & Ji, G. (1995). Age estimation from the permanent molar in northeast China by the method of average stage of attrition. Forensic Science International, 75(2-3), 189–196. doi:10.1016/0379-0738(95)01791-7 Visual method
  • 15. © 2016 GENXPLORE Radiological method • Schour and Masseler method (1941) • Nolla’s method (1960) • Moorees, Fanning and Hunt method (1963) • Demirjian & Willem method • Coronal Pulp Cavity Index • Kvaal et al REFERENCE: Dental radiographic indicators, a key to age estimation AS Panchbhai Dentomaxillofacial Radiology 2011 40:4, 199-212
  • 16. © 2016 GENXPLORE Chemical Method Amino acid racemisation
  • 17. © 2016 GENXPLORE Histological method -Incremental lines of cementum
  • 18. © 2016 GENXPLORE Translucency method Root Dentin Translucency Conventional Calliper Method Bang, G., & Ramm, E. (1970). Determination of Age in Humans from Root Dentin Transparency. Acta Odontologica Scandinavica, 28(1), 3– 35. doi:10.3109/00016357009033130
  • 19. © 2016 GENXPLORE SEX DETERMINATION • Morphological method • Molecular method • Radiological method • Microscopic method 19
  • 20. © 2016 GENXPLORE Morphological method REFERENCE: Gupta B, Gupta M. Sex identification in forensic odontology- a review of various methodology. Int J Forensic Odontol 2016;1:9- 13. 20
  • 21. © 2016 GENXPLORE MANDIBULAR CANINE INDEX •MCI in identification of sex has never exceeded 87.5%it can only be used as asupplemental tool. REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J PharmBioall Sci 2015;7:S398-402. 21
  • 22. © 2016 GENXPLORE SEX DETERMINATION BY ROOT LENGTH AND CROWN DIAMETER • -80%accuracy REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J PharmBioall Sci 2015;7:S398-402. 22
  • 23. © 2016 GENXPLORE REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J PharmBioall Sci 2015;7:S398-402. 23
  • 24. © 2016 GENXPLORE REFERENCE: Ramakrishnan K, Sharma S, Sreeja C, Pratima DB, Aesha I, Vijayabanu B. Sex determination in forensic odontology: A review. J PharmBioall Sci 2015;7:S398-402. 24
  • 26. © 2016 GENXPLORE Gonial Angle Measurements as Predictors of Sex REFERENCE: Taleb NSA, Beshlawy ME (2015) Mandibular Ramus and Gonial Angle Measurements as Predictors of Sex and Age in an Egyptian Population Sample: A Digital Panoramic Study. J Forensic Res 6: 308. doi: 10.4172/2157-7145.1000308 26
  • 27. © 2016 GENXPLORE Mandibular ramus: An indicator for sex determination REFERENCE: Saini V, Srivastava R, Rai RK, Shamal SN, Singh TB, Tripathi SK. Mandibular ramus: An indicator for sex in fragmentary mandible.J Forensic Sci 2011;56(Suppl1):S13-6. 27
  • 28. © 2016 GENXPLORE Microscopic methods Barr bodies • Presence or absence of X chromosome can be studied from buccal smears, skin biopsy, blood, cartilage, hair root sheath, and tooth pulp REFERENCE: Barr ML, Bertram LF, Lindsay HA. The morphology of the nerve cell nucleus, according to sex. Anat Rec 1950;107:283-97 28
  • 30. © 2016 GENXPLORE Sex determination using Barr bodies 30
  • 31. © 2016 GENXPLORE Molecular method from Pulpal tissue REFERENCE:Das N, Gorea RK, Gargi J, Singh JR. Sex determination from pulpal tissue. J Indian Acad Forensic Med 2004;26:50-4. 31
  • 32. © 2016 GENXPLORE Sex determination using polymerase chain reaction REFERENCE: Tsuchimochi T, Iwasa M, Maeno Y, Koyama H, Inoue H, Isobe I, et al. Chelating resin-based extraction of DNA from dental pulp and sex determination from incinerated teeth with Y-chromosomal alphoid repeat and short tandem repeats. Am J Forensic Med Pathol 2002;23:268-71. 32
  • 33. © 2016 GENXPLORE BITE MARK ANALYSIS •Scaled photography of bite mark evidence •Scaled photography of dental casts •Clinical comparisons •Computer comparisons REFERENCE: MacDonald DG. Bite mark recognition and interpretation. J Forensic Sci Soc. 1974 Jul; 14(3): 229 33 33
  • 35. © 2016 GENXPLORE Double Swabbing Technique
  • 38. © 2016 GENXPLORE Teeth Impressions REFERENCE: MacDonald DG. Bite mark recognition and interpretation. J Forensic Sci Soc. 1974 Jul; 14(3): 229 33 38
  • 39. © 2016 GENXPLORE REFERENCE: Naru AS, Dykes E . The use of a digital imaging technique to aid bite mark analysis. Sci Justice. 1996 JanMar; 36(1): 4750 39
  • 40. © 2016 GENXPLORE REFERENCESweet D, Bowers CM. Accuracy of bite mark overlays: a comparison of five common methods to produce exemplars from a suspect’s dentition. J Forensic40
  • 41. © 2016 GENXPLORE REFERENCE: Webster G. A suggested classification of bite marks in foodstuffs in forensic dental analysis. Forensic SciInt. 1982 JulAug; 20(1): 4552. 41
  • 43. © 2016 GENXPLORE Race determination REFERENCE: Edgar HJH. Prediction of race using characteristics of dental morphology. J Forensic Sci. 2005; 50(2): 269–73. REFERENCE: Lasker GW LM. Racial traits in human teeth. J Forensic Sci. 1957; 2: 401–149. 43
  • 44. © 2016 GENXPLORE Species determination REFERENCE: B. Rai, J. Kaur. Evidence-Based Forensic Dentistry. Springer-Verlag Berlin Heidelberg; 2013. DNA technology and Forensic Odontology. REFERENCE: Handbook of Forensic Medicine. First Edition. John Wiley & Sons, Ltd; 2014. 44
  • 45. © 2016 GENXPLORE RUGOSCOPY REFERENCE: Thomas CJ, Kotze TJ. The palatal rugae pattern: A new classification. J Dent. Assoc. S. Afr. 1983;38:153–157. 45
  • 46. © 2016 GENXPLORE REFERENCE: Ohtani M, Nishida N, Chiba T, Fukuda M, et al. Indication and limitations of using palatal rugae for personal identification in edentulous cases. REFERENCE: M Virdi, Y Singh, A Kumar. Role of Palatal Rugae in Forensic Identification of the Pediatric Population. The Internet Journal of Forensic Science. 2009 Volume 4 Number 2 46
  • 47. © 2016 GENXPLORE CHEILOSCOPY REFERENCE: Tsuchihashi Y. Studies on personal identification by means of lip prints. Forensic Sci. 1974 Jun;3(3):233–248. 47
  • 48. © 2016 GENXPLORE Dental Record REFERENCE: American Academy of Pediatric Dentistry. Guideline on Record keeping. Pediatr Dent. 2004; 34(special issue): 132-136. 48 DENTURE MARKING REFERENCE :Colvenkar SS. Lenticular card: A new method for denture identifi cation. Indian J Dent Res 2010;21:112-4.
  • 54. © 2016 GENXPLORE COMPARISON OF DENTAL RECORDS AND ANOMALIES IN THE TEETH REFERENCE: Krishan, K., Kanchan, T., & Garg, A. K. (2015). Dental Evidence in Forensic Identification – An Overview, Methodology and Present Status. The Open Dentistry Journal, 9, 250–256. http://doi.org/10.2174/1874210601509010250 54
  • 55. © 2016 GENXPLORE REFERENCE: Krishan, K., Kanchan, T., & Garg, A. K. (2015). Dental Evidence in Forensic Identification – An Overview, Methodology and Present Status. The Open Dentistry Journal, 9, 250–256. http://doi.org/10.2174/1874210601509010250 55
  • 57. © 2016 GENXPLORE Identification of the victims •In natural and man made disaster, acts of violence may it be traffic accidents or human right violation 57
  • 58. © 2016 GENXPLORE Identification in Mass Disaster Tsunami Flood Earthquake 58
  • 59. © 2016 GENXPLORE Accidental Disasters Flight accident Train accident Road traffic accident 59
  • 60. © 2016 GENXPLORE INTENTIONAL MASS DISASTERS Bomb blast World trade center attack in 2001 60
  • 61. © 2016 GENXPLORE Role of the FO in identification of victims SEARCH & RECOVERY PM EXAMINATION AM DATA COLLECTION RECONCILIATION DEBRIEFING 61
  • 62. © 2016 GENXPLORE References 1. Davis Oldham: 'Evidence' (English 101 & 102) at Shoreline Community College, shoreline.edu “https://app.shoreline.edu/doldham/SRR.html” Accessed 10th may 2020 2. https://blog.ipleaders.in/different-kinds-of-evidence/ Accessed 10th may 2020
  • 63. © 2016 GENXPLORE Thank You…… Visit : facebook.com/legalsaviour youtube.com/legalsaviour Follow, like and subscribe for latest updates