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--ACTIVE SITES
--PROSTHETIC GROUP
--FACTORS INFLUENCING ENZYME ACTIVITY
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PROSTHETIC GROUP
 A prosthetic group is the non-amino acid component that
is part of the structure of the heteroproteins or conjugated
proteins, being covalently linked to the apoprotein.
 This is a component of a conjugated protein that is
required for the protein's biological activity.
 The prosthetic group may be organic (such as
a vitamin, sugar, RNA, phosphate or lipid)
or inorganic (such as a metal ion).
 Prosthetic groups are bound tightly to proteins and may
even be attached through a covalent bond. They often play
an important role in enzyme catalysis.
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A protein without its prosthetic group is called an apoprotein,
while a protein combined with its prosthetic group is called
a holoprotein. A non-covalently bound prosthetic group cannot
generally be removed from the holoprotein without denaturating
the protein.
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PROSTHETIC GROUP EXAMPLE
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FACTORS INFLUENCING
ENZYME ACTIVITY
1. TEMPERATURE
2. pH
3. CONCENTRATION OF ENZYME
4. CONCENTRATION OF SUBSTRATE
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EFFECTS OF TEMPERATURE
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SUBSTRATE CONCENTRATION
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REFERENCE
 WWW.WIKEPEDIA.COM
 MICROBIOLOGY BY MICHAEL.J.PELCZAR Jr
 SHOMU’S BIOLOGY
 MICROBENOTES.COM
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