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International Journal of Modern Research in Engineering & Management (IJMREM)
||Volume|| 1||Issue|| 2 ||Pages|| 10-24 ||February 2018|| ISSN: 2581-4540
www.ijmrem.com IJMREM Page 10
Effect of dietary fibers from mango peels and date seeds on
physicochemical properties and bread quality of Arabic bread
Sam Al-Dalalia, b, c*
, Fuping Zhenga
, Salah Aleidc
, Mahmoud Abu-Ghoushd
,
Murad Samhourie
, Ammar AL-Fargaa
a
School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048,
China.
b
Department of Food Science and Technology, Ibb University, Ibb, Yemen.
c
Department of Food and Nutrition Sciences, King Faisal University, Saudi Arabia.
d
Department of Clinical Nutrition and Dietetics, The Hashemite University, Zarqa, Jordan.
e
Industrial Engineering Department, German- Jordanian University
---------------------------------------------------ABSTRACT-------------------------------------------------------
The present study aims at evaluating effects of dietary fibers of Mango peels (MP) and Date seeds (DS) on the
quality of Arabic bread (AB). MP was added at two levels (2% and 4%) and DS were at 4% and 6%, based on
flour weight. Results showed that DS is considered as a good source of dietary fiber compared to MP. Also, it was
found that MP at different levels improved the overall quality of AB. An adaptive neuro-fuzzy inference system
(ANFIS) is used to study the properties AB with the different proportions of mango peel (M) and dates seed (D)
as inputs, and two output properties (crust color CC and crumb texture CT). Experimental validation runs were
conducted to compare the measured values and the predicted ones. The comparison shows that the adoption of
this neuro- modeling technique (i.e., ANFIS) achieved a satisfactory prediction accuracy of about 85%.
KEYWORDS: Arabic bread, Dietary Fiber, Chemical Composition, physical properties, Fuzzy modeling
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Date of Submission: Date, 03 February 2018 Date of Accepted: 19 February 2018
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I. INTRODUCTION
Arabic bread (pita bread) is the most common type of bread used in Saudi Arabia in particular, and East Asia
countries in general. It has two layers, a round shape, crust color which is golden brown and it forms pocket during
baking (Qarooni., 1990). Arabic bread is made from simple ingredients including flour, salt, yeast and water.
However, the manufactures, sometimes, use optional ingredients such as fat, skim milk powder and certain
additives as emulsifiers, enzymes, dietary fiber and preservatives (Mir et al., 2014; AL-Dmoor., 2012; Abu
Ghoush et al., 2008a; Abu Ghoush et al., 2008b).
Recently, the demand for food, especially that is rich in dietary fiber (DF), is increasing (Ajila et al., 2008),
because DF maintains health and protects human body against diseases (Ajila et al., 2008; Criqui and Ringel.,
1994). Bread, which is formulated with functional ingredients, has become essential in the bakery industry, and
the functional bread is already available (Menrad., 2003). It was found that using of fiber non-cereal sources
receives the interest of many researchers. Almana and Mahmoud., (1994) found that date seeds might be used at
10% replacement level in wheat flour to produce Saudi Mafrood flat bread without any significant adverse effects
on quality. Anil., (2007) reported that using hydrated hazelnut testa at level of 10% in breadmaking is acceptable.
Ajila et al. (2008) used successfully mango peel as a source of dietary fiber in producing biscuits. Also, Wang et
al., (2002) used carob fiber, inulin and pea fiber in breadmaking. Other fibers sources such as potato peel and
apple fiber were added to bread, muffin and cookies without any adverse effect (Toma et al.,1979; Chen et al.,
1988). Mango is one of the most cultivated fruit in the world. (FAO., 2009) reported that mango fruits production
reached more than 35 million tons. During manufacturing mango to other products such as puree, nectar, leather,
pickles and canned slices, peel is a major by- products which contributes about 15 – 20% of the fruits (Ashoush
and Gadallah., 2011; Loelillet., 1994; Beerh et al., 1976). Vergara Valencia et al. (2007) reported that mango DF
concentrate contained low lipid, high starch content and balanced soluble dietary fiber/insoluble dietary fiber
levels. AL-Sheraji et al., (2011) stated that dietary fiber from Mangifera pajang Kort can be used as a suitable
source to enhance nutritional value for bakery products. Ashoush and Gadallah., (2011) and Ajila et al., (2008)
found that incorporating biscuits dough with mango peel was acceptable until 10% with mango flavour.
Effect of dietary fibers from mango peels and date…
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Almana and Mahmoud., (1994) state "palm dates (Phoenix dactylifera) are a major fruit crop in Saudi Arabia".
The production of date in Saudi Arabia reached 1.031 million tons (Year Agriculture Statistical Book., 2013). The
seeds are produced in large amount in the Middle East a by-products of date industry, which represent about 10 –
12% from the fruit (Platat et al., 2014; Platat et al., 2015). Date seeds are used in human or animal feed (Almana
and Mahmoud., 1994). Bouaziz et al., (2010) found that date seed fiber concentrate of two varieties, (i.e. Allig
and Deglet Nour) when defatted, they increased the dietary fiber content when added to bread with slight adverse
effects on bread quality.
Fuzzy logic and fuzzy inference system (FIS) is an effective technique for the identification and modeling of
complex nonlinear systems. Fuzzy logic is particularly attractive due to its ability to solve problems in the absence
of accurate mathematical models. The prediction of properties of the wheat flour (i.e., crust color and crumb
texture) is considered complex system, so using the conventional technology to model these properties results in
significant discrepancies between simulation results and experimental data. Thus, this complex nonlinear system
fits within the realm of neuro-fuzzy techniques. The application of a neuro-fuzzy inference system to prediction
and modeling is a novel approach that overcomes limitations of a fuzzy inference system such as the dependency
on the expert for fuzzy rule generation and design of the non-adaptive fuzzy set. Modeling and identification of
food properties and processing has been the subject of many researchers in the food engineering field. Perrot et
al., (2003) presented a hybrid approach based on fuzzy logic and genetic algorithms to control a cross flow
microfiltration pilot plant. The results of simulations and pilot tests showed that it becomes possible to impose
dynamics to the process that leads to maintain the state variable at a given reference. Tsourveloudis and Kiralakis.,
(2002) applied a rotary drying process to olive stones. They described and modeled the process using fuzzy and
neuro-fuzzy techniques based on available expertise and knowledge for a given, industrial size, rotary dryer. They
also used ANFIS controller based on data taken from an empirical model of the dryer under study.
Apple grading using fuzzy logic was introduced by (Kavdir and Guyer., 2003). Fuzzy logic was applied as a
decision making support to grade apples. Grading results obtained from fuzzy logic showed 89% general
agreement with results obtained from human expert, providing good flexibility in reflecting the expert's
expectations and grading standards into the results. No previous studies were done to evaluate effect of MP and
DS addition on physical and chemical properties of Arabic bread quality. Therefore, the present study aims at
evaluating the effect of dietary fiber of MP and DS on the quality of Arabic bread and applying neuro fuzzy
modeling for prediction of crumb crust color and crumb texture of the bread.
II. MATERIAL AND METHODS
Material: Hard wheat flour from Yecora Rojo variety (Extraction rate 75%) was obtained from the local market
in Al-Ahsa, produced by Grain Silos and Flour Mills Organization; Dammam Saudi Arabia in 2014. Commercial
safe-instant dry compressed yeast (Manufactured in France by S.I.Lesaffre59703 Marcq France) and commercial
iodized table salt (locally purchased) were used in this study. From preliminary trials, MP (2% and 4%), DS (4%
and 6%) and their mix (1:1) have better bread quality.
Preparation of fiber sources :MP was prepared according to (Ajila et al., 2008), whereas DS was prepared
according to (Almana and Mahmoud, 1994). However, a 200 mm sieve was used in this study.
Chemical composition :Wheat flour, MP and DS were analyzed in order to account for their moisture, protein,
fat and ash, according to the methods described in (AOAC., 2006). Carbohydrate was calculated by differences
as following: Total carbohydrate% = {100 – (moisture% + ash% + protein% + fat%)}.
Total dietary fiber (TDF), soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were determined according
to the method described by (Ajila and Prasada Rao., 2013).
Soluble sugar was determined using HPLC with refractive index detection, following the method described by
(AOAC., 2006). Standard solutions were prepared from various sugar (glucose 12 mg/mL, lactose 20 mg/mL,
sucrose 12 mg/mL, maltose 20 mg/mL and fructose 32 mg/mL) and injected in HPLC Instrument (Agilent 1260
infinity, Germany), column was (Zorbax Carbohydrate Analysis, 4.6mm ID x 250 mm 5μm), mobil phase (75/25
Acetonitrile/water), Flow Rate (1.4ml/min), Temperature (30°C), Detector (HP1100 RID, 30°C), sample volume
(50 μL in 50/50 Acetonitrile/water), retention time (20min).
Physical characteristics of flour and dough :Wet gluten, dry gluten and gluten index were determined using
(AACC., 1995) standard method 38-12, using (Gluten Index Glutamatic, Perten, Instrument, Sweden). Falling
number was determined using (AACC., 1995) 56–81B (Falling number apparatus Model 1700, Perten Instrument
manual, Hagberg, Sweden).
Effect of dietary fibers from mango peels and date…
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Arabic bread baking : Arabic bread was made according to Qarooni et al., (1987). In brief, flour 700g, salt 10.5g
and instant active dry yeast 3.5g were mixed for 1 min using lab mixer (Tekno stamp, Italy). Water was added
with mixing for 2 min at low speed. Then, 2 min at medium speed. Finally, 4 min at high speed. After one hour
of bulk fermentation at 30˚C and 90 – 95% relative humidity, the dough was divided into 80g pieces, manually
rounded and left for 15 min. following the same previous conditions. Dough pieces were sheeted (2mm thickness).
All sheeted doughs were kept in proofing cabinet for 30 min. at the same condition. The proofed dough pieces
were kept on a hot solid aluminum tray, and baked at 450-500˚C for 45–55 seconds. The baked loaves were cooled
for 15min., wrapped in plastic sacks and stored at room temperature.
Sensory Evaluation : Evaluation of Arabic bread quality was performed by sensory assessment according to
(Qarooni et al., 1989) after 1 hour and 24 hours of baking.
Crust and Crumb Color: Crust and Crumb color was determined using a Hunterlab color meter (Hunterlab,
Hunter Associates Laboratory, Inc. Reston, VA. USA). The color differences (ΔΕ) were calculated by the
following equation ΔE=√ (ΔL)2
+ (Δa)2
+ (Δb)2
.
Fuzzy Modeling of Output Properties : Neuro-fuzzy is an associative memory system that consists of fuzzy
nodes instead of simple input and output nodes. Neuro-fuzzy uses neural network learning functions to refine each
part of the fuzzy knowledge separately. Learning in a separated network is faster than learning in a whole network.
One approach to the derivation of a fuzzy rule base is to use the self learning features of artificial neural networks,
to define the membership function based on input-output data. A fuzzy inference system (consisting of rules, fuzzy
set membership functions, and the defuzzification strategy) are mapped onto a neural network-like architecture.
Adaptive neuro-fuzzy inference system (ANFIS) is a fuzzy inference system implemented in the framework of an
adaptive neural network. By using a hybrid learning procedure, ANFIS can construct an input-output mapping
based on both human-knowledge as fuzzy. If-Then rules and stipulated input-output data pairs for neural networks
training. ANFIS architecture is shown in Figure 1., where x and y are the inputs, f is the output, Ai and A2
n are the
input membership functions, wi and w2
n are the rules firing strengths. Five network layers are used by ANFIS to
perform the following fuzzy inference steps: (i) input fuzzification, (ii) fuzzy set database construction, (iii) fuzzy
rule base construction (iv) decision making, and (v) output defuzzification. This is a multi-layered neural network
architecture where the first layer represents the antecedent fuzzy sets, while the consequent fuzzy sets are
represented by the middle layers, and the defuzzification strategy by the output layer. The nodes which have
'square' shape are those containing adaptable parameters, whereas the 'circular' nodes are those with fixed
parametes.
ANFIS is more powerful than the simple fuzzy logic algorithm and neural networks, since it provides a method
for fuzzy modeling to learn information about the data set, in order to compute the membership function
parameters that best allow the associated fuzzy inference system to track the given input/output data (Jang., 1993).
ANFIS also employs sugeno-type fuzzy inference system, which is a natural and efficient modeling tool, and is
suited for modeling non-linear system by interpolating between multiple linear models. In addition, ANFIS is
more powerful than neural network system since it is better than all of them in convergence rates (running time),
average training error, root mean square error, and the coefficient of correlation, and it has a built-in ability to
validate the modeled system. On the other hand, ANFIS is much more complex than the fuzzy inference systems,
and is not available for all of the fuzzy inference system options. It only has a single output, and no rule sharing.
In addition, ANFIS cannot accept all the customization options that basic fuzzy inference allows. That is, no
possibility to make our own membership functions and defuzzification functions; the ones provided by ANFIS
must be used.
ANFIS Modeling of Crust Color and Crumb Texture : An adaptive neuro-fuzzy inference system (ANFIS) is
an architecture which is functionally equivalent to a Sugeno-type fuzzy rule base. ANFIS is a method for tuning
an existing rule base with a learning algorithm based on a collection of training data. This allows the rule base to
adapt. Training data is used to teach the neuro-fuzzy system by adapting its parameters (which in essence are
fuzzy set membership function parameters) and using a standard neural network algorithm which utilizes a
gradient search, such that the mean square output error is minimized.
The architecture of ANFIS, illustrated in Figure 1 has five layers to accomplish the tuning process of the fuzzy
modeling system. The five layers are:
1) Layer 1: Every node in this layer is an adaptive node with a node function (i.e., membership function).
Parameters of membership functions are referred to as premise or antecedent parameters.
Effect of dietary fibers from mango peels and date…
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2) Layer 2: Every node in this layer is a fixed node, which multiplies the incoming signals and sends the product
out. Each node represents the firing strength of a fuzzy rule.
3) Layer 3: Every node in this layer is a fixed node which calculates the ratio of the one firing strength to the
sum of all rules' firing strengths. The outputs of this layer are called normalized firing strengths.
4) Layer 4: Every node in this layer is an adaptive node with a node function (i.e., linear combination of input
variables). Parameters in this layer are referred to as consequent parameters.
5) Layer 5: The single node in this layer is a fixed node that computes the overall output as the summation of
all incoming signals, shown in Figure (1), it is observed that given the values of premise parameters, the
overall output can be expressed as a linear combination of the consequent parameters.
ANFIS applies two techniques in updating parameters. For premise parameters that define membership functions,
ANFIS employs gradient descent back-propagation neural networks to fine-tune them. For consequent parameters
that define the coefficient of each output equation, ANFIS uses that least squares method to identify them. This
approach is called the hybrid learning method. More specifically, in the forward pass of the hybrid learning
method, functional signals go forward until layer 4 and the consequent parameters are identified by the least square
estimate. In the backward pass, the error rates propagate backward and the premise parameters are updated by the
gradient descent.
ANFIS modeling and prediction of the output properties (i.e., crust color (CC), and crumb texture (CT)) starts by
obtaining a data set (input-output data points) and dividing it into training and validating data sets. Each
input/output pair contains two inputs (i.e., Proportion of Mango peel (M), and proportion of date seed (D)) and
two outputs (i.e., crust color (CC) and crumb texture (CT)). The training data set is used to find the initial premise
parameters for the membership functions by equally spacing each of the membership functions. A threshold value
for error between the actual and desired output is determined. The consequent parameters are computed using the
least squares method. Then, an error for each data pairs is found. If this error is larger than the threshold value,
the premise parameters are updated using the back propagation neural networks. This process is terminated when
the error becomes less than the threshold value. Then, the testing data points are used to compare the model with
actual system for validating purposes.
Statistical Analysis
All data were expressed as mean values ± SD. Statistical analysis was performed using one way analysis of
variance (ANOVA). Comparisons between means were tested by Duncan’s multiple range test with p≤ 0.05 being
considered statistically significant. Statistical analysis was conducted with SAS software (SAS., 2002).
III. RESULTS AND DISCUSSION
Chemical composition of flour and dietary fiber sources :The results of chemical analysis of wheat flour and
DF sources are shown in table (1). Wheat flour exhibited the highest levels of protein and moisture compared to
MP and DS. MP exhibited the highest level of ash compared to wheat flour and DS. On the other hand, fat content
was the highest in DS compared to wheat flour and MP. Flour with protein content of 9 – 12% is optimum for
Arabic bread (Quail et al., 1991; Qarooni et al., 1988). Aleid et al., (2014) reported that Saudi flours with 80%
and 95% extraction exhibited 12.9 and 14.3% protein content respectively. Ashoush and Gadallah., (2011) found
that MP powder exhibited 4.92 % moisture, 3.88 % ash, 1.23 % fat and 3.60 % protein. Nehdi et al., (2010)
reported that DS exhibited 10.20 % moisture, 10.36 %fat, 1.18 % ash and 5.67 % protein. Sugar content of the
wheat flour, MP and DS are shown in (Table 2). Wheat flour showed the presence of glucose, sucrose and maltose,
whereas MP contained glucose, fructose, galactose, sucrose and maltose in large amount compared to wheat flour
and DS.
DS showed the presence of fructose, glucose and sucrose. All soluble sugar were detected in MP (Table 2).
However, Galactose was not detected in both DS and wheat flour (Table 2). Hassan et al., (2011) found that fiber-
rich powder from (Mangifera pajang K) had mannose, arabinose, glucose, fructose and Galactose. TDF content
in DS (64.75%) was higher than TDF in MP (29.22%) on dry weight basis as shown in Table (3). Almana and
Mahmoud., (1994) found that the TDF content was around 71 and 80% for fine and coarse fractions of DS
respectively. In MP, SDF was 13.59% and IDF was 15.28% (Table 3). The SDF/IDF ratio is considered to be a
well-balanced proportion for maximum health benefits (Schneeman., 1987). In this study MP shows a good
balance in SDF/IDF ratio. Ajila et al., (2008) mentioned that wheat bran showed 6.6% SDF which is very low
compared to that of MP, which supports the fact that addition of fruit fiber to cereal bakery products will enhance
the nutritional quality compared to cereal bran sources.
Effect of dietary fibers from mango peels and date…
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Physical characteristics of flour and dough : It was found that flour (75% extraction) exhibited wet gluten
(28.35 g), dry gluten (10 g) and gluten index (98.01%) as shown in Table (4). The flour falling number was 384
s. Aleid et al., (2014) found that the falling number was 447 and 492 s for Saudi flour with 80% and 95% extraction
respectively. Brain., (2005) reported that a falling number value of 350 s or high indicates low enzyme activity,
while value below 200 s indicates high level of enzyme activity.
Quality of Arabic Bread with Fiber Sources :Effect of MP and DS dietary fibers on Arabic bread quality can
be shown in Tables (5 and 6).
Size, Shape and Smoothness : There were no significant differences in size and shape values among all
treatments. Arabic bread with DS levels of 4 and 6% exhibited significant effect (p≤0.05) in Arabic bread
smoothness compared to other treatments (Table 5).
Crust Color, Blisters and Pocketing : The tested DF showed more significant effect (p≤0.05) in Arabic bread
crust color. DS exhibited significant effect (p≤0.05) on crust color (dark color) at 4 and 6% levels, whereas MP
and the mix showed less effect (Table 5). There was no significant effect in blisters and pocketing among all
treatments, except DS at level 6% Table (5). Almana and Mahmoud., (1994) found that milled date seed fraction
adversely affected bread color more than coarse milled date seed fraction or wheat bran.
Ability of Rolling and Folding on First Day and Second Day : The ability of rolling and folding of Arabic
bread was evaluated in the first and second days. There was no significant effect on rolling and folding for control,
MP (2 and 4%), DS (4%) and the mix treatments on the first day, while the same treatments showed significant
reduction (p≤0.05) on the second day with exception of MP at level 2% and control (Tables 5 and 6). Arabic bread
with DS at level 6% showed significant effect (p≤0.05) in first and second days on the rolling and folding
characters.
Crumb Texture, Crumb Appearance and Evenness of Layers : There was no significant effect on crumb
texture, crumb appearance and evenness of layers among all treatments (p≤0.05) except DS at level 6% (Table 6).
Tearing on First Day and Second Day : The ability of Arabic bread for tearing was evaluated on first and second
day. MP showed no significant effect in Arabic bread tearing on first and second days compared to control. While
DS (4 and 6%) and MIX showed significant effect (p≤0.05) in Arabic bread tearing on first and second day (Table
6).
Total Score : Data in Table (6) showed that addition of MP at 2 and 4% levels were similar to control in total
score. Treatments with DS (4%) and the mix gave less values than MP, whereas DS at 6% showed significant
effect (p≤0.05) on Arabic bread total score. Artz et al., (1990); Ellis., (1985); Chen et al., (1988); Almana and
Mahmoud., (1994) found that some problems may appear with different high fiber sources addition on baked
products, such as lower volume, color, odor, bitter taste and other adverse effect on quality. Almana and
Mahmoud., (1994) reported that coarse DS fraction can be used at 10% replacement in bread making without
significant adverse effect on quality. Ajila et al., (2008) and Ashoush and Gadallah., (2011) found that wheat
flour incorporated with 10% MP were acceptable with mango flavor.
Crust and Crumb Color: The effect of fiber sources addition on Arabic bread crust color was evaluated using
Hunter lab (Table 7). All treatments with MP, DS and the mix significantly reduced the lightness (L). The highest
reduction was observed in DS at level 6% (p≤0.05). There was no significant effect on (a) value for all treatment,
with exception DS at both 4 % and 6 % levels that showed significant reduction (p≤0.05) in (a) value compared
to all treatments. The b value was decreased with adding different fiber sources. The highest reduction was
observed on DS at level 6%. These changes in crust color may be due to Millard and caramelization reaction
(Anil., 2007). Ajila et al., (2008) reported that this change is due to the enzymatic browning.
In crumb color, there was significant decrease (p≤0.05) in (L) value with addition of fiber sources, more effect
was observed in treatment with addition DS at level 6% as shown in Table (8). There was significant increase
(p≤0.05) in (a) value with all treatment compared to control. Yellowness (b values) showed significant increase
(p≤0.05) with the addition of MP (2 and 4%) and the mix compared to control, whereas DS (4 and 6%) showed
significant decrease (p≤0.05) compared to control. Bouaziz et al., (2010) found that color of the bread crumb was
not dramatically affected by the substitution of coarse fraction (280µm ˂ particle size ≤600 µm) from defatted
date seeds.
Effect of dietary fibers from mango peels and date…
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ANFIS Modeling : The fuzzy logic toolbox of Matlab 7.0 was used to obtain the results, and to build a fuzzy
model for the CC and CT. Figures (2 and 3) show the training curves for building fuzzy models for CC and CT.
42 data points were used for training the system to predict each property. 100 neural nets learning epochs were
used in both systems to get a low error of training (i.e., RMSE = 0.72 or 15% for CC, and 0.68 or 14% for CT).
The comparisons between the actual and ANFIS predicted CC and CT after training are shown in Figures (4 and
5, respectively), which show that the systems are well-trained to model the actual CC and CT values.Ten data
points, which are different and independent from the training data, were used to validate the systems. The final
fuzzy inference systems that predict the CC and CT are shown in Figures (6 and 7, respectively). As illustrated in
Figures (8 and 9), a two (Gaussian) type membership functions for each input (2 inputs) resulted in high accurate
prediction results for both CC and CT. The final fuzzy-based prediction model for CC and CT are illustrated in
Figures (10 and 11, respectively).
Models Validation :The ANFIS prediction models for crust color (CC) and crumb texture (CT) were validated
by selecting a certain number of data points (i.e., 10 points), different from the other 42 points used for ANFIS
training. Each validation data point (i.e., Mango peel (M) and dates seed (D)) was fed into the system, and then
the predicted properties (i.e., CC and CT) were compared to the actual values of the measured CC and CT values.
The average percent errors in the modeling of CC and CT were 15% and 14% for CC and CT respectively,
achieving an accuracy of CC prediction of 85%, and of CT prediction of 86%. Table 9 shows the data points used
in system's validation along with the actual and predicted CC and CT values, and the percent errors in the
predictions. This table shows that the ANFIS predicted values are a close match of the actual ones.
IV. CONCLUSIONS
DS showed a significant content of dietary fiber, whereas MP came in second rank. The TDF content in MP
showed a balanced IDF/SDF level, which is important for the maximum health benefits. Addition of MP and DS
increased water absorption and dough stability compared to control. An increase in MTI due to DS addition was
observed. However, MP showed a decreasing effect on MTI compared to control. The highest overall Arabic
bread total quality score was observed with the addition of MP (2% and 4%), DS (4%) and MIX (MP+ DS). In
contrast, DS at 6% decreased Arabic bread total score (overall acceptability). ANFIS models achieved an overall
average prediction error of wheat flour crust color and crumb texture of only 15%. The present study shows that
ANFIS is a technique that can be used efficiently to predict the food properties. It is believed that this approach
can be applied to predict many other parameters and properties in food industry.
ACKNOLEDGMENTS
The researchers acknowledge gratefully the financial support from the Deanship of Scientific Research, King
Faisal University, Saudi Arabia. Project #152006.
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Table (1) Chemical composition of flour and dietary fiber sources
Values are means of triplicates (±) standard deviations.
Results were expressed as % dry mater except moisture.
Table (2) soluble sugar composition of the wheat flour, MP and DS
Date
seed
Mango PeelWheat FlourTreatment
3.82
(±0.02)
11.59
(±0.77)
n.d.Fructose
3.14
(±0.01)
1.7
(±0.02)
0.065
(±0.006)
Glucose
n.d.0.54
(±0.05)
n.d.Galactose
2.33
(±0.07)
27.6
(±2.25)
0.35
(±0.008)
Sucrose
n.d.0.64
(±0.02)
0.33
(±0.01)
Maltose
n.d. Represents not detected.
Values are means of triplicates (±) standard deviations.
Results were expressed as % dry mater.
Date seedMango PeelWheat FlourTreatment*
6.29
(±0.13)
9.45
(±0.47)
12.94
(±0.13)
Moisture
2.26
(±0.02)
5.1
(±0.14)
0.63
(±0.004)
Ash
6.34
(±0.05)
5.27
(±0.17)
13.14
(±0.11)
Protein
11.61
(±0.03)
6.5
(±0.69)
3.26
(±0.43)
Fat
79.79
(±0.03)
83.13
(±.0.21)
82.97
(±0.11)
Carbohydrate
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 18
Table (3) Composition of TDF, SDF and IDF in MP and DS
n.d. Represents not detected.
Values are means of triplicates (±) standard deviations.
Results were expressed as % dry mater.
Table (4) Physical characteristics of flour and dough
Values are means of triplicates
(±) standard deviations and calculation based on 14 % moisture basis.
Table (5) Effect of fiber sources on the external properties of Arabic bread
Within each column means with same letter(s) are not significantly different at P<0.05
Values are means of triplicates ± standard deviations.
Date seedMango PeelTreatment
64.75
(± 0.12)
13.59
(± 0.1)
INSOLUBLE DIETARY FIBER
n.d
15.28
(± 0.11)
SOLUBLE DIETARY FIBER
64.75
(± 0.12)
29.22
(±0.1)
TOTAL DIETARY FIBER
Falling NO.
(sec)
Gluten Index
(%)
Passed Thru G.I Sieve
(g)
Dry Gluten (g)Wet Gluten
(g)
384
(±14.8)
98.01
(±0.02)
0.05310
(±0.00)
28.35
(±1.06)
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 19
Table (6) Effect of fiber sources on the internal properties of Arabic bread
Within each column means with same letter(s) are not significantly different at P<0.05
Values are means of triplicates ± standard deviations.
Table (7) Effect of fiber sources on the crust color of Arabic bread
Within each column means with same letter(s) are not significantly different at P<0.05.
L scale : Light vs. dark, where a low number (0-50) indicates dark and a high number (51-100) indicates Light,;
a scale : Red vs. green, where a positive number indicates red and a negative number indicates green,; b scale :
Yellow vs. blue, where a positive number indicates yellow and a negative number indicates blue,; ΔE (Delta E) :
Total color difference, ΔE=√ (ΔL)2
+ (Δa)2
+ (Δb)2
.
Crust Color
ΔEYellowness(b)Redness(a)Lightness(L)Conc.
(%)
Treatment
34.58±0.50a
11.29±0.84bcd
68.39±1.96a
0control
8.899±0.76d
31.70±0.86b
12.58±1.14ab
60.09±1.67b
2Mango Peel
(MP) 11.878±0.67c
31.71±0.21b
13.45±0.91a
57.07±2.36b
4
14.037±0.83b
24.32±0.28d
10.59±0.27d
59.04±0.66b
4Date Seed
(DS) 19.460±1.22a
21.63±0.85e
10.93±0.60cd
53.98±1.53c
6
13.351±0.79cb
27.33±0.38c
12.13±0.10abc
57.32±0.74b
2 : 2MIX(MP+DS)
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 20
Table (8) Effect of fiber sources on the crumb color of Arabic bread
Crumb color
ΔEYellowness(b)Redness(a)Lightness(L)Conc. (%)Treatment
20.29±1.31c
1.59±0.28f
75.93±1.68a
0control
11.729±1.47d
22.19±0.28b
6.53±0.12e
65.51±0.34b
2Mango Peel
(MP) 14.335±1.83cd
26.72±0.14a
7.37±0.11d
64.51±0.56b
4
19.247±1.47b
18.01±0.31d
7.91±0.21c
57.94±0.56d
4Date Seed
(DS) 23.677±1.26a
17.56±0.07d
9.05±0.03b
53.66±0.44e
6
17.066±1.73cb
21.84±0.27b
9.61±0.11a
61.00±0.70c
2 : 2MIX(MP+DS)
Within each column means with same letter(s) are not significantly different at P<0.05.
L scale : Light vs. dark, where a low number (0-50) indicates dark and a high number (51-100) indicates Light,;
a scale : Red vs. green, where a positive number indicates red and a negative number indicates green,; b scale :
Yellow vs. blue, where a positive number indicates yellow and a negative number indicates blue,; ΔE (Delta E) :
Total color difference, ΔE=√ (ΔL)2
+ (Δa)2
+ (Δb)2
.
Table (9) Validation Data
Figure 1. ANFIS Architecture
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 21
Figure 2. Learning curve of crust color fuzzy system
Figure 3. Learning curve of crumb texture fuzzy system
Figure 4. Actual and ANFIS-predicted values of crust color
Figure 5. Actual and ANFIS-predicted values of crumb texture
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 22
Figure 6. Fuzzy Inference System of the crust color
Figure 7. Fuzzy Inference System of the crumb texture
Figure 8. Final fuzzy membership functions for the crust color
0 1 2 3 4 5 6
0
0.2
0.4
0.6
0.8
1
D
Degreeofmembership
Low High
0 0.5 1 1.5 2 2.5 3 3.5 4
0
0.2
0.4
0.6
0.8
1
M
Degreeofmembership
Low High
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 23
Figure 9. Final fuzzy membership functions for the crumb texture
Figure 10. The final fuzzy-based prediction model of crust color
0 0.5 1 1.5 2 2.5 3 3.5 4
0
0.2
0.4
0.6
0.8
1
M
Degreeofmembership
Low High
0 1 2 3 4 5 6
0
0.2
0.4
0.6
0.8
1
D
Degreeofmembership
Low High
Effect of dietary fibers from mango peels and date…
www.ijmrem.com IJMREM Page 24
Figure 11. The final fuzzy-based prediction model of crumb texture

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Effect of dietary fibers from mango peels and date seeds on physicochemical properties and bread quality of Arabic bread

  • 1. International Journal of Modern Research in Engineering & Management (IJMREM) ||Volume|| 1||Issue|| 2 ||Pages|| 10-24 ||February 2018|| ISSN: 2581-4540 www.ijmrem.com IJMREM Page 10 Effect of dietary fibers from mango peels and date seeds on physicochemical properties and bread quality of Arabic bread Sam Al-Dalalia, b, c* , Fuping Zhenga , Salah Aleidc , Mahmoud Abu-Ghoushd , Murad Samhourie , Ammar AL-Fargaa a School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China. b Department of Food Science and Technology, Ibb University, Ibb, Yemen. c Department of Food and Nutrition Sciences, King Faisal University, Saudi Arabia. d Department of Clinical Nutrition and Dietetics, The Hashemite University, Zarqa, Jordan. e Industrial Engineering Department, German- Jordanian University ---------------------------------------------------ABSTRACT------------------------------------------------------- The present study aims at evaluating effects of dietary fibers of Mango peels (MP) and Date seeds (DS) on the quality of Arabic bread (AB). MP was added at two levels (2% and 4%) and DS were at 4% and 6%, based on flour weight. Results showed that DS is considered as a good source of dietary fiber compared to MP. Also, it was found that MP at different levels improved the overall quality of AB. An adaptive neuro-fuzzy inference system (ANFIS) is used to study the properties AB with the different proportions of mango peel (M) and dates seed (D) as inputs, and two output properties (crust color CC and crumb texture CT). Experimental validation runs were conducted to compare the measured values and the predicted ones. The comparison shows that the adoption of this neuro- modeling technique (i.e., ANFIS) achieved a satisfactory prediction accuracy of about 85%. KEYWORDS: Arabic bread, Dietary Fiber, Chemical Composition, physical properties, Fuzzy modeling --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: Date, 03 February 2018 Date of Accepted: 19 February 2018 --------------------------------------------------------------------------------------------------------------------------------------- I. INTRODUCTION Arabic bread (pita bread) is the most common type of bread used in Saudi Arabia in particular, and East Asia countries in general. It has two layers, a round shape, crust color which is golden brown and it forms pocket during baking (Qarooni., 1990). Arabic bread is made from simple ingredients including flour, salt, yeast and water. However, the manufactures, sometimes, use optional ingredients such as fat, skim milk powder and certain additives as emulsifiers, enzymes, dietary fiber and preservatives (Mir et al., 2014; AL-Dmoor., 2012; Abu Ghoush et al., 2008a; Abu Ghoush et al., 2008b). Recently, the demand for food, especially that is rich in dietary fiber (DF), is increasing (Ajila et al., 2008), because DF maintains health and protects human body against diseases (Ajila et al., 2008; Criqui and Ringel., 1994). Bread, which is formulated with functional ingredients, has become essential in the bakery industry, and the functional bread is already available (Menrad., 2003). It was found that using of fiber non-cereal sources receives the interest of many researchers. Almana and Mahmoud., (1994) found that date seeds might be used at 10% replacement level in wheat flour to produce Saudi Mafrood flat bread without any significant adverse effects on quality. Anil., (2007) reported that using hydrated hazelnut testa at level of 10% in breadmaking is acceptable. Ajila et al. (2008) used successfully mango peel as a source of dietary fiber in producing biscuits. Also, Wang et al., (2002) used carob fiber, inulin and pea fiber in breadmaking. Other fibers sources such as potato peel and apple fiber were added to bread, muffin and cookies without any adverse effect (Toma et al.,1979; Chen et al., 1988). Mango is one of the most cultivated fruit in the world. (FAO., 2009) reported that mango fruits production reached more than 35 million tons. During manufacturing mango to other products such as puree, nectar, leather, pickles and canned slices, peel is a major by- products which contributes about 15 – 20% of the fruits (Ashoush and Gadallah., 2011; Loelillet., 1994; Beerh et al., 1976). Vergara Valencia et al. (2007) reported that mango DF concentrate contained low lipid, high starch content and balanced soluble dietary fiber/insoluble dietary fiber levels. AL-Sheraji et al., (2011) stated that dietary fiber from Mangifera pajang Kort can be used as a suitable source to enhance nutritional value for bakery products. Ashoush and Gadallah., (2011) and Ajila et al., (2008) found that incorporating biscuits dough with mango peel was acceptable until 10% with mango flavour.
  • 2. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 11 Almana and Mahmoud., (1994) state "palm dates (Phoenix dactylifera) are a major fruit crop in Saudi Arabia". The production of date in Saudi Arabia reached 1.031 million tons (Year Agriculture Statistical Book., 2013). The seeds are produced in large amount in the Middle East a by-products of date industry, which represent about 10 – 12% from the fruit (Platat et al., 2014; Platat et al., 2015). Date seeds are used in human or animal feed (Almana and Mahmoud., 1994). Bouaziz et al., (2010) found that date seed fiber concentrate of two varieties, (i.e. Allig and Deglet Nour) when defatted, they increased the dietary fiber content when added to bread with slight adverse effects on bread quality. Fuzzy logic and fuzzy inference system (FIS) is an effective technique for the identification and modeling of complex nonlinear systems. Fuzzy logic is particularly attractive due to its ability to solve problems in the absence of accurate mathematical models. The prediction of properties of the wheat flour (i.e., crust color and crumb texture) is considered complex system, so using the conventional technology to model these properties results in significant discrepancies between simulation results and experimental data. Thus, this complex nonlinear system fits within the realm of neuro-fuzzy techniques. The application of a neuro-fuzzy inference system to prediction and modeling is a novel approach that overcomes limitations of a fuzzy inference system such as the dependency on the expert for fuzzy rule generation and design of the non-adaptive fuzzy set. Modeling and identification of food properties and processing has been the subject of many researchers in the food engineering field. Perrot et al., (2003) presented a hybrid approach based on fuzzy logic and genetic algorithms to control a cross flow microfiltration pilot plant. The results of simulations and pilot tests showed that it becomes possible to impose dynamics to the process that leads to maintain the state variable at a given reference. Tsourveloudis and Kiralakis., (2002) applied a rotary drying process to olive stones. They described and modeled the process using fuzzy and neuro-fuzzy techniques based on available expertise and knowledge for a given, industrial size, rotary dryer. They also used ANFIS controller based on data taken from an empirical model of the dryer under study. Apple grading using fuzzy logic was introduced by (Kavdir and Guyer., 2003). Fuzzy logic was applied as a decision making support to grade apples. Grading results obtained from fuzzy logic showed 89% general agreement with results obtained from human expert, providing good flexibility in reflecting the expert's expectations and grading standards into the results. No previous studies were done to evaluate effect of MP and DS addition on physical and chemical properties of Arabic bread quality. Therefore, the present study aims at evaluating the effect of dietary fiber of MP and DS on the quality of Arabic bread and applying neuro fuzzy modeling for prediction of crumb crust color and crumb texture of the bread. II. MATERIAL AND METHODS Material: Hard wheat flour from Yecora Rojo variety (Extraction rate 75%) was obtained from the local market in Al-Ahsa, produced by Grain Silos and Flour Mills Organization; Dammam Saudi Arabia in 2014. Commercial safe-instant dry compressed yeast (Manufactured in France by S.I.Lesaffre59703 Marcq France) and commercial iodized table salt (locally purchased) were used in this study. From preliminary trials, MP (2% and 4%), DS (4% and 6%) and their mix (1:1) have better bread quality. Preparation of fiber sources :MP was prepared according to (Ajila et al., 2008), whereas DS was prepared according to (Almana and Mahmoud, 1994). However, a 200 mm sieve was used in this study. Chemical composition :Wheat flour, MP and DS were analyzed in order to account for their moisture, protein, fat and ash, according to the methods described in (AOAC., 2006). Carbohydrate was calculated by differences as following: Total carbohydrate% = {100 – (moisture% + ash% + protein% + fat%)}. Total dietary fiber (TDF), soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were determined according to the method described by (Ajila and Prasada Rao., 2013). Soluble sugar was determined using HPLC with refractive index detection, following the method described by (AOAC., 2006). Standard solutions were prepared from various sugar (glucose 12 mg/mL, lactose 20 mg/mL, sucrose 12 mg/mL, maltose 20 mg/mL and fructose 32 mg/mL) and injected in HPLC Instrument (Agilent 1260 infinity, Germany), column was (Zorbax Carbohydrate Analysis, 4.6mm ID x 250 mm 5μm), mobil phase (75/25 Acetonitrile/water), Flow Rate (1.4ml/min), Temperature (30°C), Detector (HP1100 RID, 30°C), sample volume (50 μL in 50/50 Acetonitrile/water), retention time (20min). Physical characteristics of flour and dough :Wet gluten, dry gluten and gluten index were determined using (AACC., 1995) standard method 38-12, using (Gluten Index Glutamatic, Perten, Instrument, Sweden). Falling number was determined using (AACC., 1995) 56–81B (Falling number apparatus Model 1700, Perten Instrument manual, Hagberg, Sweden).
  • 3. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 12 Arabic bread baking : Arabic bread was made according to Qarooni et al., (1987). In brief, flour 700g, salt 10.5g and instant active dry yeast 3.5g were mixed for 1 min using lab mixer (Tekno stamp, Italy). Water was added with mixing for 2 min at low speed. Then, 2 min at medium speed. Finally, 4 min at high speed. After one hour of bulk fermentation at 30˚C and 90 – 95% relative humidity, the dough was divided into 80g pieces, manually rounded and left for 15 min. following the same previous conditions. Dough pieces were sheeted (2mm thickness). All sheeted doughs were kept in proofing cabinet for 30 min. at the same condition. The proofed dough pieces were kept on a hot solid aluminum tray, and baked at 450-500˚C for 45–55 seconds. The baked loaves were cooled for 15min., wrapped in plastic sacks and stored at room temperature. Sensory Evaluation : Evaluation of Arabic bread quality was performed by sensory assessment according to (Qarooni et al., 1989) after 1 hour and 24 hours of baking. Crust and Crumb Color: Crust and Crumb color was determined using a Hunterlab color meter (Hunterlab, Hunter Associates Laboratory, Inc. Reston, VA. USA). The color differences (ΔΕ) were calculated by the following equation ΔE=√ (ΔL)2 + (Δa)2 + (Δb)2 . Fuzzy Modeling of Output Properties : Neuro-fuzzy is an associative memory system that consists of fuzzy nodes instead of simple input and output nodes. Neuro-fuzzy uses neural network learning functions to refine each part of the fuzzy knowledge separately. Learning in a separated network is faster than learning in a whole network. One approach to the derivation of a fuzzy rule base is to use the self learning features of artificial neural networks, to define the membership function based on input-output data. A fuzzy inference system (consisting of rules, fuzzy set membership functions, and the defuzzification strategy) are mapped onto a neural network-like architecture. Adaptive neuro-fuzzy inference system (ANFIS) is a fuzzy inference system implemented in the framework of an adaptive neural network. By using a hybrid learning procedure, ANFIS can construct an input-output mapping based on both human-knowledge as fuzzy. If-Then rules and stipulated input-output data pairs for neural networks training. ANFIS architecture is shown in Figure 1., where x and y are the inputs, f is the output, Ai and A2 n are the input membership functions, wi and w2 n are the rules firing strengths. Five network layers are used by ANFIS to perform the following fuzzy inference steps: (i) input fuzzification, (ii) fuzzy set database construction, (iii) fuzzy rule base construction (iv) decision making, and (v) output defuzzification. This is a multi-layered neural network architecture where the first layer represents the antecedent fuzzy sets, while the consequent fuzzy sets are represented by the middle layers, and the defuzzification strategy by the output layer. The nodes which have 'square' shape are those containing adaptable parameters, whereas the 'circular' nodes are those with fixed parametes. ANFIS is more powerful than the simple fuzzy logic algorithm and neural networks, since it provides a method for fuzzy modeling to learn information about the data set, in order to compute the membership function parameters that best allow the associated fuzzy inference system to track the given input/output data (Jang., 1993). ANFIS also employs sugeno-type fuzzy inference system, which is a natural and efficient modeling tool, and is suited for modeling non-linear system by interpolating between multiple linear models. In addition, ANFIS is more powerful than neural network system since it is better than all of them in convergence rates (running time), average training error, root mean square error, and the coefficient of correlation, and it has a built-in ability to validate the modeled system. On the other hand, ANFIS is much more complex than the fuzzy inference systems, and is not available for all of the fuzzy inference system options. It only has a single output, and no rule sharing. In addition, ANFIS cannot accept all the customization options that basic fuzzy inference allows. That is, no possibility to make our own membership functions and defuzzification functions; the ones provided by ANFIS must be used. ANFIS Modeling of Crust Color and Crumb Texture : An adaptive neuro-fuzzy inference system (ANFIS) is an architecture which is functionally equivalent to a Sugeno-type fuzzy rule base. ANFIS is a method for tuning an existing rule base with a learning algorithm based on a collection of training data. This allows the rule base to adapt. Training data is used to teach the neuro-fuzzy system by adapting its parameters (which in essence are fuzzy set membership function parameters) and using a standard neural network algorithm which utilizes a gradient search, such that the mean square output error is minimized. The architecture of ANFIS, illustrated in Figure 1 has five layers to accomplish the tuning process of the fuzzy modeling system. The five layers are: 1) Layer 1: Every node in this layer is an adaptive node with a node function (i.e., membership function). Parameters of membership functions are referred to as premise or antecedent parameters.
  • 4. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 13 2) Layer 2: Every node in this layer is a fixed node, which multiplies the incoming signals and sends the product out. Each node represents the firing strength of a fuzzy rule. 3) Layer 3: Every node in this layer is a fixed node which calculates the ratio of the one firing strength to the sum of all rules' firing strengths. The outputs of this layer are called normalized firing strengths. 4) Layer 4: Every node in this layer is an adaptive node with a node function (i.e., linear combination of input variables). Parameters in this layer are referred to as consequent parameters. 5) Layer 5: The single node in this layer is a fixed node that computes the overall output as the summation of all incoming signals, shown in Figure (1), it is observed that given the values of premise parameters, the overall output can be expressed as a linear combination of the consequent parameters. ANFIS applies two techniques in updating parameters. For premise parameters that define membership functions, ANFIS employs gradient descent back-propagation neural networks to fine-tune them. For consequent parameters that define the coefficient of each output equation, ANFIS uses that least squares method to identify them. This approach is called the hybrid learning method. More specifically, in the forward pass of the hybrid learning method, functional signals go forward until layer 4 and the consequent parameters are identified by the least square estimate. In the backward pass, the error rates propagate backward and the premise parameters are updated by the gradient descent. ANFIS modeling and prediction of the output properties (i.e., crust color (CC), and crumb texture (CT)) starts by obtaining a data set (input-output data points) and dividing it into training and validating data sets. Each input/output pair contains two inputs (i.e., Proportion of Mango peel (M), and proportion of date seed (D)) and two outputs (i.e., crust color (CC) and crumb texture (CT)). The training data set is used to find the initial premise parameters for the membership functions by equally spacing each of the membership functions. A threshold value for error between the actual and desired output is determined. The consequent parameters are computed using the least squares method. Then, an error for each data pairs is found. If this error is larger than the threshold value, the premise parameters are updated using the back propagation neural networks. This process is terminated when the error becomes less than the threshold value. Then, the testing data points are used to compare the model with actual system for validating purposes. Statistical Analysis All data were expressed as mean values ± SD. Statistical analysis was performed using one way analysis of variance (ANOVA). Comparisons between means were tested by Duncan’s multiple range test with p≤ 0.05 being considered statistically significant. Statistical analysis was conducted with SAS software (SAS., 2002). III. RESULTS AND DISCUSSION Chemical composition of flour and dietary fiber sources :The results of chemical analysis of wheat flour and DF sources are shown in table (1). Wheat flour exhibited the highest levels of protein and moisture compared to MP and DS. MP exhibited the highest level of ash compared to wheat flour and DS. On the other hand, fat content was the highest in DS compared to wheat flour and MP. Flour with protein content of 9 – 12% is optimum for Arabic bread (Quail et al., 1991; Qarooni et al., 1988). Aleid et al., (2014) reported that Saudi flours with 80% and 95% extraction exhibited 12.9 and 14.3% protein content respectively. Ashoush and Gadallah., (2011) found that MP powder exhibited 4.92 % moisture, 3.88 % ash, 1.23 % fat and 3.60 % protein. Nehdi et al., (2010) reported that DS exhibited 10.20 % moisture, 10.36 %fat, 1.18 % ash and 5.67 % protein. Sugar content of the wheat flour, MP and DS are shown in (Table 2). Wheat flour showed the presence of glucose, sucrose and maltose, whereas MP contained glucose, fructose, galactose, sucrose and maltose in large amount compared to wheat flour and DS. DS showed the presence of fructose, glucose and sucrose. All soluble sugar were detected in MP (Table 2). However, Galactose was not detected in both DS and wheat flour (Table 2). Hassan et al., (2011) found that fiber- rich powder from (Mangifera pajang K) had mannose, arabinose, glucose, fructose and Galactose. TDF content in DS (64.75%) was higher than TDF in MP (29.22%) on dry weight basis as shown in Table (3). Almana and Mahmoud., (1994) found that the TDF content was around 71 and 80% for fine and coarse fractions of DS respectively. In MP, SDF was 13.59% and IDF was 15.28% (Table 3). The SDF/IDF ratio is considered to be a well-balanced proportion for maximum health benefits (Schneeman., 1987). In this study MP shows a good balance in SDF/IDF ratio. Ajila et al., (2008) mentioned that wheat bran showed 6.6% SDF which is very low compared to that of MP, which supports the fact that addition of fruit fiber to cereal bakery products will enhance the nutritional quality compared to cereal bran sources.
  • 5. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 14 Physical characteristics of flour and dough : It was found that flour (75% extraction) exhibited wet gluten (28.35 g), dry gluten (10 g) and gluten index (98.01%) as shown in Table (4). The flour falling number was 384 s. Aleid et al., (2014) found that the falling number was 447 and 492 s for Saudi flour with 80% and 95% extraction respectively. Brain., (2005) reported that a falling number value of 350 s or high indicates low enzyme activity, while value below 200 s indicates high level of enzyme activity. Quality of Arabic Bread with Fiber Sources :Effect of MP and DS dietary fibers on Arabic bread quality can be shown in Tables (5 and 6). Size, Shape and Smoothness : There were no significant differences in size and shape values among all treatments. Arabic bread with DS levels of 4 and 6% exhibited significant effect (p≤0.05) in Arabic bread smoothness compared to other treatments (Table 5). Crust Color, Blisters and Pocketing : The tested DF showed more significant effect (p≤0.05) in Arabic bread crust color. DS exhibited significant effect (p≤0.05) on crust color (dark color) at 4 and 6% levels, whereas MP and the mix showed less effect (Table 5). There was no significant effect in blisters and pocketing among all treatments, except DS at level 6% Table (5). Almana and Mahmoud., (1994) found that milled date seed fraction adversely affected bread color more than coarse milled date seed fraction or wheat bran. Ability of Rolling and Folding on First Day and Second Day : The ability of rolling and folding of Arabic bread was evaluated in the first and second days. There was no significant effect on rolling and folding for control, MP (2 and 4%), DS (4%) and the mix treatments on the first day, while the same treatments showed significant reduction (p≤0.05) on the second day with exception of MP at level 2% and control (Tables 5 and 6). Arabic bread with DS at level 6% showed significant effect (p≤0.05) in first and second days on the rolling and folding characters. Crumb Texture, Crumb Appearance and Evenness of Layers : There was no significant effect on crumb texture, crumb appearance and evenness of layers among all treatments (p≤0.05) except DS at level 6% (Table 6). Tearing on First Day and Second Day : The ability of Arabic bread for tearing was evaluated on first and second day. MP showed no significant effect in Arabic bread tearing on first and second days compared to control. While DS (4 and 6%) and MIX showed significant effect (p≤0.05) in Arabic bread tearing on first and second day (Table 6). Total Score : Data in Table (6) showed that addition of MP at 2 and 4% levels were similar to control in total score. Treatments with DS (4%) and the mix gave less values than MP, whereas DS at 6% showed significant effect (p≤0.05) on Arabic bread total score. Artz et al., (1990); Ellis., (1985); Chen et al., (1988); Almana and Mahmoud., (1994) found that some problems may appear with different high fiber sources addition on baked products, such as lower volume, color, odor, bitter taste and other adverse effect on quality. Almana and Mahmoud., (1994) reported that coarse DS fraction can be used at 10% replacement in bread making without significant adverse effect on quality. Ajila et al., (2008) and Ashoush and Gadallah., (2011) found that wheat flour incorporated with 10% MP were acceptable with mango flavor. Crust and Crumb Color: The effect of fiber sources addition on Arabic bread crust color was evaluated using Hunter lab (Table 7). All treatments with MP, DS and the mix significantly reduced the lightness (L). The highest reduction was observed in DS at level 6% (p≤0.05). There was no significant effect on (a) value for all treatment, with exception DS at both 4 % and 6 % levels that showed significant reduction (p≤0.05) in (a) value compared to all treatments. The b value was decreased with adding different fiber sources. The highest reduction was observed on DS at level 6%. These changes in crust color may be due to Millard and caramelization reaction (Anil., 2007). Ajila et al., (2008) reported that this change is due to the enzymatic browning. In crumb color, there was significant decrease (p≤0.05) in (L) value with addition of fiber sources, more effect was observed in treatment with addition DS at level 6% as shown in Table (8). There was significant increase (p≤0.05) in (a) value with all treatment compared to control. Yellowness (b values) showed significant increase (p≤0.05) with the addition of MP (2 and 4%) and the mix compared to control, whereas DS (4 and 6%) showed significant decrease (p≤0.05) compared to control. Bouaziz et al., (2010) found that color of the bread crumb was not dramatically affected by the substitution of coarse fraction (280µm ˂ particle size ≤600 µm) from defatted date seeds.
  • 6. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 15 ANFIS Modeling : The fuzzy logic toolbox of Matlab 7.0 was used to obtain the results, and to build a fuzzy model for the CC and CT. Figures (2 and 3) show the training curves for building fuzzy models for CC and CT. 42 data points were used for training the system to predict each property. 100 neural nets learning epochs were used in both systems to get a low error of training (i.e., RMSE = 0.72 or 15% for CC, and 0.68 or 14% for CT). The comparisons between the actual and ANFIS predicted CC and CT after training are shown in Figures (4 and 5, respectively), which show that the systems are well-trained to model the actual CC and CT values.Ten data points, which are different and independent from the training data, were used to validate the systems. The final fuzzy inference systems that predict the CC and CT are shown in Figures (6 and 7, respectively). As illustrated in Figures (8 and 9), a two (Gaussian) type membership functions for each input (2 inputs) resulted in high accurate prediction results for both CC and CT. The final fuzzy-based prediction model for CC and CT are illustrated in Figures (10 and 11, respectively). Models Validation :The ANFIS prediction models for crust color (CC) and crumb texture (CT) were validated by selecting a certain number of data points (i.e., 10 points), different from the other 42 points used for ANFIS training. Each validation data point (i.e., Mango peel (M) and dates seed (D)) was fed into the system, and then the predicted properties (i.e., CC and CT) were compared to the actual values of the measured CC and CT values. The average percent errors in the modeling of CC and CT were 15% and 14% for CC and CT respectively, achieving an accuracy of CC prediction of 85%, and of CT prediction of 86%. Table 9 shows the data points used in system's validation along with the actual and predicted CC and CT values, and the percent errors in the predictions. This table shows that the ANFIS predicted values are a close match of the actual ones. IV. CONCLUSIONS DS showed a significant content of dietary fiber, whereas MP came in second rank. The TDF content in MP showed a balanced IDF/SDF level, which is important for the maximum health benefits. Addition of MP and DS increased water absorption and dough stability compared to control. An increase in MTI due to DS addition was observed. However, MP showed a decreasing effect on MTI compared to control. The highest overall Arabic bread total quality score was observed with the addition of MP (2% and 4%), DS (4%) and MIX (MP+ DS). In contrast, DS at 6% decreased Arabic bread total score (overall acceptability). ANFIS models achieved an overall average prediction error of wheat flour crust color and crumb texture of only 15%. The present study shows that ANFIS is a technique that can be used efficiently to predict the food properties. It is believed that this approach can be applied to predict many other parameters and properties in food industry. ACKNOLEDGMENTS The researchers acknowledge gratefully the financial support from the Deanship of Scientific Research, King Faisal University, Saudi Arabia. Project #152006. REFERENCES 1. AACC. (1995). Approved methods of the AACC. St. Paul, MN.U.S.A. 2. Abu Ghoush, M., Herald, T., Dowell, F., Xie, F., Aramouni, F., & Madl, R. (2008a). Effect Of Preservatives Addition On The Shelf Life Extensions And Quality Of Flat Bread As Determined By Near Infrared Spectroscopy And Texture Analysis. International Journal of Food Science and Technology, 43, 357-364. 3. Abu Ghoush, M., Herald, T., Dowell, F., & Xie, F. (2008b). Effect of antimicrobial agents and dough conditioners on the shelf-life extension and quality of flat bread, as determined by near-infrared spectroscopy. 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  • 8. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 17 41. Sudha, M. L., Vetrimani, R. A., & Leelavathi, K. (2007). Influence of fibre from different cereals on the rheological characteristics of wheat flour dough and on biscuit quality. Food Chemistry, 100, 1365-1370. 42. Toma, R. B., Orr, P. H., D'appolonia, B., Dintzis, F. R., & Tabekhia, M. M. (1979). Physical and chemical properties of potato peel as a source of dietary fiber in bread. J. Food Sci, 44, 1403-1407. 43. Tsourveloudis, N., & Kiralakis, L. (2002). “Rotary Drying of Olive Stones: Fuzzy Modeling and Control”, Department of Production Engineering and Management, Technical University of Crete, BA3015. 44. Vergara Valencia, N., Granados perez, E., Agama Acevedo, E., Tovar, J., Ruales, J., & Bello perez, L. A. (2007). Fiber concentrate from mango fruit: Characterization, associated antioxidant capacity and application as a bakery product ingredient. LWT - Food Science and Technology, 40, 722-729. 45. Wang, J., Rosell, C. M., & Barber, C. B. D. (2002). Effect of the addition of different fibres on wheat dough performance and bread quality. Food Chemistry, 79, 221–226. 46. Year Agriculture Statistical Book. (2013). Twenty six Issue. Ministry of Agriculture. Kingdom of Saudi Arabia, 6-290. Table (1) Chemical composition of flour and dietary fiber sources Values are means of triplicates (±) standard deviations. Results were expressed as % dry mater except moisture. Table (2) soluble sugar composition of the wheat flour, MP and DS Date seed Mango PeelWheat FlourTreatment 3.82 (±0.02) 11.59 (±0.77) n.d.Fructose 3.14 (±0.01) 1.7 (±0.02) 0.065 (±0.006) Glucose n.d.0.54 (±0.05) n.d.Galactose 2.33 (±0.07) 27.6 (±2.25) 0.35 (±0.008) Sucrose n.d.0.64 (±0.02) 0.33 (±0.01) Maltose n.d. Represents not detected. Values are means of triplicates (±) standard deviations. Results were expressed as % dry mater. Date seedMango PeelWheat FlourTreatment* 6.29 (±0.13) 9.45 (±0.47) 12.94 (±0.13) Moisture 2.26 (±0.02) 5.1 (±0.14) 0.63 (±0.004) Ash 6.34 (±0.05) 5.27 (±0.17) 13.14 (±0.11) Protein 11.61 (±0.03) 6.5 (±0.69) 3.26 (±0.43) Fat 79.79 (±0.03) 83.13 (±.0.21) 82.97 (±0.11) Carbohydrate
  • 9. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 18 Table (3) Composition of TDF, SDF and IDF in MP and DS n.d. Represents not detected. Values are means of triplicates (±) standard deviations. Results were expressed as % dry mater. Table (4) Physical characteristics of flour and dough Values are means of triplicates (±) standard deviations and calculation based on 14 % moisture basis. Table (5) Effect of fiber sources on the external properties of Arabic bread Within each column means with same letter(s) are not significantly different at P<0.05 Values are means of triplicates ± standard deviations. Date seedMango PeelTreatment 64.75 (± 0.12) 13.59 (± 0.1) INSOLUBLE DIETARY FIBER n.d 15.28 (± 0.11) SOLUBLE DIETARY FIBER 64.75 (± 0.12) 29.22 (±0.1) TOTAL DIETARY FIBER Falling NO. (sec) Gluten Index (%) Passed Thru G.I Sieve (g) Dry Gluten (g)Wet Gluten (g) 384 (±14.8) 98.01 (±0.02) 0.05310 (±0.00) 28.35 (±1.06)
  • 10. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 19 Table (6) Effect of fiber sources on the internal properties of Arabic bread Within each column means with same letter(s) are not significantly different at P<0.05 Values are means of triplicates ± standard deviations. Table (7) Effect of fiber sources on the crust color of Arabic bread Within each column means with same letter(s) are not significantly different at P<0.05. L scale : Light vs. dark, where a low number (0-50) indicates dark and a high number (51-100) indicates Light,; a scale : Red vs. green, where a positive number indicates red and a negative number indicates green,; b scale : Yellow vs. blue, where a positive number indicates yellow and a negative number indicates blue,; ΔE (Delta E) : Total color difference, ΔE=√ (ΔL)2 + (Δa)2 + (Δb)2 . Crust Color ΔEYellowness(b)Redness(a)Lightness(L)Conc. (%) Treatment 34.58±0.50a 11.29±0.84bcd 68.39±1.96a 0control 8.899±0.76d 31.70±0.86b 12.58±1.14ab 60.09±1.67b 2Mango Peel (MP) 11.878±0.67c 31.71±0.21b 13.45±0.91a 57.07±2.36b 4 14.037±0.83b 24.32±0.28d 10.59±0.27d 59.04±0.66b 4Date Seed (DS) 19.460±1.22a 21.63±0.85e 10.93±0.60cd 53.98±1.53c 6 13.351±0.79cb 27.33±0.38c 12.13±0.10abc 57.32±0.74b 2 : 2MIX(MP+DS)
  • 11. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 20 Table (8) Effect of fiber sources on the crumb color of Arabic bread Crumb color ΔEYellowness(b)Redness(a)Lightness(L)Conc. (%)Treatment 20.29±1.31c 1.59±0.28f 75.93±1.68a 0control 11.729±1.47d 22.19±0.28b 6.53±0.12e 65.51±0.34b 2Mango Peel (MP) 14.335±1.83cd 26.72±0.14a 7.37±0.11d 64.51±0.56b 4 19.247±1.47b 18.01±0.31d 7.91±0.21c 57.94±0.56d 4Date Seed (DS) 23.677±1.26a 17.56±0.07d 9.05±0.03b 53.66±0.44e 6 17.066±1.73cb 21.84±0.27b 9.61±0.11a 61.00±0.70c 2 : 2MIX(MP+DS) Within each column means with same letter(s) are not significantly different at P<0.05. L scale : Light vs. dark, where a low number (0-50) indicates dark and a high number (51-100) indicates Light,; a scale : Red vs. green, where a positive number indicates red and a negative number indicates green,; b scale : Yellow vs. blue, where a positive number indicates yellow and a negative number indicates blue,; ΔE (Delta E) : Total color difference, ΔE=√ (ΔL)2 + (Δa)2 + (Δb)2 . Table (9) Validation Data Figure 1. ANFIS Architecture
  • 12. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 21 Figure 2. Learning curve of crust color fuzzy system Figure 3. Learning curve of crumb texture fuzzy system Figure 4. Actual and ANFIS-predicted values of crust color Figure 5. Actual and ANFIS-predicted values of crumb texture
  • 13. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 22 Figure 6. Fuzzy Inference System of the crust color Figure 7. Fuzzy Inference System of the crumb texture Figure 8. Final fuzzy membership functions for the crust color 0 1 2 3 4 5 6 0 0.2 0.4 0.6 0.8 1 D Degreeofmembership Low High 0 0.5 1 1.5 2 2.5 3 3.5 4 0 0.2 0.4 0.6 0.8 1 M Degreeofmembership Low High
  • 14. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 23 Figure 9. Final fuzzy membership functions for the crumb texture Figure 10. The final fuzzy-based prediction model of crust color 0 0.5 1 1.5 2 2.5 3 3.5 4 0 0.2 0.4 0.6 0.8 1 M Degreeofmembership Low High 0 1 2 3 4 5 6 0 0.2 0.4 0.6 0.8 1 D Degreeofmembership Low High
  • 15. Effect of dietary fibers from mango peels and date… www.ijmrem.com IJMREM Page 24 Figure 11. The final fuzzy-based prediction model of crumb texture