2. Our experience of 40 Years brings innovation to you
UltraSafe® Class II biological safety cabinets deliver maximum safety, comfort
and convenience. Featuring an advanced design for easy operation and maximum safety,
our UltraSafe® cabinets may be used wherever protection is of paramount importance in
safety levels 1 to 3, including research, pharmaceutical quality control labs, pharmacies,
and clinics/hospitals.
• Superior Personnel and Product Protection with best-in-class air flow and filter technology.
• Exceptionally Comfortable ergonomic design prevents user fatigue and promotes safe
working habits.
• Maximized Energy Efficiency for environmental protection and significant cost savings.
Features.
1. A clever interlocking switch design prevents accidental exposure to UV light. Should the front
window be opened whilst the cabinet is in use an override program ensures your safety by
automatically adopting a class 1 bio-safety mode. This feature will auto-cancel when the front
window is closed again.
2. The modern, proven control panel provides one touch access to all functions including servicing.
A fully integrated self-diagnostic processor with digital status display backed up by an audible and
visual alarm guarantees your safety.
3. An internally mounted, exhaust HEPA filter adds to the stylish features and modern design of the
cabinet by freeing the outer shell from protrusions.
The supply filter provides ISO Class 3 (per ISO14644.1) and US Class 100 clean air to the work surface in a
gentle vertical laminar flow for product protection.
4. The work area is large, airy and bright. Light pours in through large toughened glass sides and a wide
pneumatically assisted front window.
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3. UltraSafe®.
5. An ergonomically designed sloping front
and new flat front intake grille allows for optimum
operator comfort and prolonged use with ease.
With the BSC work surface height at 900mm above
the floor, and the seat heights set as above, the reach
onto and over the work surface can be estimated
when reaching into the cabinet at the arm extension
of 120°. Generally speaking, the farther a person can
reach into the BSC without awkward positioning, the greater flexibility and access that person has to the interior
workspace.
These guidelines on proper seat adjustment, while relating to only one aspect of BSC ergonomics, will allow the
user to optimize the work height of the cabinet in order to maximize reach and usability of the BSC. The
UltraSafe BSC, when optimized as a complete workstation, will accommodate one of the farthest reaches into
the working chamber of any BSC offered today.
6. A large front opening (210mm) provide a comfortable and easy working access for the operator whilst
still ensuring sterility and containment.
7. Heavy gauge stainless steel and superior materials are used throughout. Direct drive fans (2 or more),
dynamically balanced for vibrations free operation, ensure large flow reserves for years of trouble-free
operation.
8. All electrical components installed during manufacturing are CE Compliant in accordance to current
EN Standards.
3
4. UltraSafe®.
Performance and reliability are guaranteed by full compliance with AS 2252 Part 2. 2009
All components meet or exceed applicable safety requirements.
Each cabinet is individually factory tested for operational performance and electrical safety.
Documentation specific to each cabinet serial number is maintained on file
The new UltraSafe® cabinets also meet and exceed the performance requirements of EN12469
& NSF 49-Annex F. safety and performance requirements.
Inflow of room air enters the front air grille to establish operator protection;
room air does not enter the work zone, preventing product contamination.
The inflow velocity, down flow velocity,
airflow path and intake geometry are
precision tuned and tested to create an optimum
air curtain on the front aperture, this curtain
maintains personal and product protection
even in the unlikely event of a severe inflow or
down flow imbalance that, would compromise
protection in a conventional cabinet.
The combination inflow and down flow air streams
forms an air barrier that prevents work surface emissions from
escaping the work zone.
Contaminated Air In
9
10
11. HEPA FILTER
HEPA FILTER
4
5. Air Barrier Containment
Maged Shenouda, 2
Sherry Randhawa
Institute of Medical and Veterinary Science, Adelaide, South Australia.
Flinders University of South Australia
Faculty of Science and Engineering, ENGR4508 Engineering Honours Project
Abstract
The aim of this project is to investigate the various methods
used indetermination air barrier containment of Biological
Safety cabinets and validate their effectiveness. The Project’s
research showed that the aerosol test used in determination
of the air barrier containment should be endorsed in the
Australian standard as a valid method of testing.
Introduction
Biological safety cabinets have proven to be an essential
item of equipments inside any research and or diagnostics
laboratories. Our dependency on them has improved
drastically. Cabinets are essential component in handling
biological hazardous materials. It is the only mean of safety
from getting infected with dangerous substance that
contribute to a high death rate that can be prevented simply
by just using proper biological safety cabinets. This
research focused on the air flow inside the cabinet and how
it could affect the air barrier at the sash of the cabinet. In
addition to establishing a methodology of validating the
Aerosol test.
Methods
Test 1
• Setting up the cabinet according to the British
standard.
• Test the cabinet using potassium Iodide Discus
method. Examine the environmental factors that
may affect the cabinet.
• Examine and observe the droplets affect of the KI.Examine
and observe the droplets affect of the KI. scrutinize the effects
of varying the velocity of the main fan motor and the exhaust
fan motor on the performance of the air barrier and on the
performance of the potassium iodide discus results.
Investigate the operator protection factor.
• On the original setting of the cabinet in accordance to the
British standard test the cabinet using the smoke generator
test (the aerosol test). Examine the outcomes and correlate
the results.
• Determine the differences and the similarities and determine
if the test pass or fail. Enhance the photometer with the use
of particle counter in order to achieve a quantitative results.
• Repeat the test with setting up a cabinet in accordance to the
Australian standard then start to test it using the potassium
iodide discus. Produce a graph of comparison of the suitable
air velocity that could be used where both tests fails/ passes.
• Outline the findings in terms of performance and air
velocities.
Aerosol liquid test
Cheaper equipment
multi-purpose
Short down time to the
cabinet
Using adequate air
velocity 0.4m/sec to
0.45m/sec
Simulating operators
movements
Testing along the whole
sash
Penetrations of smoke
particles
Particle size consistent
less than 0.3 µm
Real time scanning
KI discus test
• The cost of the equipment •
• The time taken to set up, and
the general down time to the •
cabinet
• The use of low air velocity 0.3 •
m/sec
• The metal cylinder is fixed
which doesn’t represent the •
actual action of the operator
• The localised position of the •
samplers at the centre.
• The droplet effects of un- •
evaporated KI
• Particle size range from 4 to •
10 µm
• No substance reference to •
OPF of 10^5
Test 2:
• Scattering effect around the cabinet.
• Use a die to observe the scattered potassium iodide
around the cabinet.
• Is it sufficient to test the centre only?
• Move the sampler of the KI discus towards the side of
the cabinet and carry out a test. Work out the operator
protection factor at the new positions of the samplers.
• Correlate the results obtained versus the results
obtained from testing at the centre only. Investigate the
areas where the barrier is vulnerable.
Pump
Op. virt. arm
KI nozzle.
s
N
M
n
Disc
Air samplers
Test 3:
• Work out the centripetal force that the particles hitting
the barrier with for both the KI discus method and the
aerosol liquid method.
• For the KI discus method
• 38mm spinning disc (28,000 r/min) + nozzle delivering
M=20ml of 15g/l solution of KI, generating N particles
• Air samplers s=100dm3
/min with 25mm filter
membranes
• Count the spots on developed membranes: n.
• Calculate Apf:
– with N = 3.1 x 107
x M
– Apf = = Ns/104
n
• If n = 62, Apf = 1 x 105
• If n = 1, Apf = 6.2 x 106
• Correlate with the force of generating aerosol.
Results
• By lowering the speed of the
main fan
• Average air velocity of 1.12 m/s
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Inflow airvelocity of a BSC
Inflow air at the
opening of the
cabinet
1 2 3 4 5 6 7 8 9 10
positions of the anemometer
Validation and Comparison Results
Conclusion
The Key factor or controller of the air barrier is the exhaust
fan motor. The barrier is maintained proportionally to the
adjustment of the exhaust fan as shown from the results.
lowering the air velocity of the main fan interrupt the barrier,
and raise the main issue of cross contamination inside the
cabinet. From the comparison performed, Aerosol liquid test
provide better qualitative method of testing the air barrier
since it cover lots of aspects of practical informative method
of test. KI discus test can not be carried out at any laboratory
since it could contaminate the work in the actual laboratory.
In contrast to aerosol test which doesn’t interfere with the
nature of work carried out in the laboratory.
Acknowledgement
• Miss Sherry Randhawa academic supervisor of the
research
• I.M.V.S Engineering services department.
• Clyde-Apac for their contribution and providing us with the
KI discus equipment as well as referred materials.
References
• Australian standard 2252.2.
• Australian standard 1807.26-2004
• Australian standard 1807.22
• IMVS, CTL , procedure manuals for mechanical testing.
• ESCO Global Co-operation, Singapore.
• Published article reviews on KI discus
Poster Templates photog@imvs.sa.gov.au
Now at these optimum
conditions
•Testing the inflow of the air
across the opening of the
cabinet using anemometer
at 10 labelled spots, hence
50 mm from each sides of
the cabinets then with in a
spacing of 100 mm after
the sides.
•Average air velocity of
1.7m/s
• By lowering the speed of
the exhaust fan
• Average air velocity of
0.21 m/s
Inflow air velocity of a BSC
2
1.5
Inflow air at the
1 opening of the
cabinet
0.5
0
1 2 3 4 5 6 7 8 9 10
positions of the anemometer
Inflow air velocity of a BSC
0.5
0.4
0.3 Inflow air at the
opening of the
0.2 cabinet
0.1
0
1 2 3 4 5 6 7 8 9 10
positions of the anemometer
1 2,
1
2
6. UltraSafe®.
Providing a safe barrier for you to work in.
Pressure sensor monitoring located inside the
air chamber ensures safe airflows across the
entire work surface of the UltraSafe®
cabinets.
UltraSafe® uses digital technology to
maintain constant airflow during
normal filter loading or temporary
airflow obstruction from foreign
objects
Silence without compromising comfort and
safety.
User safety and comfort is paramount with all
AES Environmental manufactured biological
safety cabinets. The comfortable, 200 mm front
working aperture on the UltraSafe™ does not
compromise safety or sample containment while
significantly reducing noise level.
All of our cabinets have been tested at nominal
airflow velocities for added security.
Customized work surfaces
Scratch‐free, high quality
stainless steel work surfaces are
available as single or
segmented modules and are
easy to clean or autoclave. The
indented work modules easily
trap spilled liquids.
The transparent side UV
resistant safety glass
maximize light and visibility
inside the cabinet, providing
a bright and open working
environment.
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10. UltraSafe®.
Options and Accessories Part Numbers
2060111 Floor stand UltraSafe 90
2060121 Floor stand UltraSafe 120
2060131 Floor stand UltraSafe 180
1687‐5512 Auto Adjustable Electric Floor Stand UltraSafe 90 & 120
1687‐5518 Auto Adjustable Electric Floor Stand UltraSafe 180
2080710 Additional Power outlet, single‐socket (GPO)
1687‐0100/1‐5 Gas Tap & Solenoid
1687‐0100/4‐5 Vacuum Tap with Millipore Filter
1687‐0100/2‐5 Air & or Vacuum Tap
1687‐0100/5‐6 20mm Granite vibration damped work surface
1687‐0100/6‐90 Modified Front Safety Window for Microscope Application UltraSafe 90
1687‐0100/6‐120 Modified Front Safety Window for Microscope Application UltraSafe 120
1687‐0100/6‐180 Modified Front Safety Window for Microscope Application UltraSafe 180
1687‐0100/7‐90 2 Piece Level Work Tray UltraSafe 90
1687‐0100/7‐120 3 Piece Level Work Tray UltraSafe 120
1687‐0100/7‐180 3 Piece Level Work Tray UltraSafe 180
8
12. KDD 9/7T 350W 4P-1 3SA
VOLT 220-240 Ph 1
WATT 350 Hz 50
RPM 1330/1150/1050 Cl. F uF/V 12.5/450 Date 11/5/01
POLE 4 Temp. min -20o
C N. Amp 2.4 Weight 12.6 kg
IP 21 max +40o
C M. Amp 3.3
All Dimension in mm .
* Sound Lp(A) at 1 m free field.
* Performance shown is for installation type B - free inlet, ducted outlet
SL. KD09003.3
Part No. CE-AD1F723.01
Wiring diagram
232 85317
152
262
327
185
39
Low
Med
Hi
Capacitor
Green-Yellow
Red
Brown
Blue
Black
White
L
N
HIGH
MEDIUM
LOW
65.858.551.4
67.2
68.1
64.1
60.7
64.7
64.4
13. AES Environmental
GENERAL SPECIFICATION ULTRASAFE ® CLASS II BIOLOGICAL SAFETY CABINET
Model(s) UltraSafe 90T UltraSafe 120 UltraSafe 180
Part Number(s) 1687‐6000/90T 1687‐6000/120T 1687‐6000/180T
Nominal Size(s) 0.9 meters (3’) 1.2 meters (4’) 1.8 meters (6’)
Cabinet External Dimensions (W x D x H) 1035 x 790 x 1570mm 1350 x 790 x 1570mm 1975 x 790 x 1570mm
Internal Work Zone Dimensions (W x D x H) 870 x 580 x 620mm 1180 x 580 x 620mm 1810 x 580 x 620mm
Test Opening Aperture 210mm
Working Opening Aperture 210mm
Fans: 240V Single Phase Direct Drive 2 2 3
1 meter per second at set point
0.4 – 0.45 meters per second
250 Litres per second 300 Litres per second 490
240 Litres per second 290 Litres per second 470
250 Litres per second 300 Litres per second 490
< 58.8 dB (A) < 60.2 dB (A) < 62.8 dB (A)
99.9995 % at 0.1 to 0.3 microns to AS: 4270/EN 1822
99.9995 % at 0.1 to 0.3 microns AS : 4270/EN1822
400mW/m²
1200 Lux
AS 1807.1 / AS 1807.5 / AS 1807.6 / 1807.15 / 1807.20 / AS 1807.22 / AS 1807.23
1.2 mm 18 gauge electro galvanized steel. Powder Coated
1.2 mm 18 gauge type 304 stainless steel with B2 finish
1.2 mm 18 gauge type 304 stainless steel with B2 finish
Gas tightness of outer shell determined in accordance with AS 1807.25
6mm Laminated Glass
1300 Watts ‐ 10 Amps
10 Amps
4.5 Amps
0.7 Kw
210
1085 x 800 x 1650mm
240
1.4322m³
WWW.AESENVIRONMENTAL.COM.AU SALES@AESENVIRONMENTAL.COM.AU
A Division of AES Environmental (SA) Pty Ltd ABN 74 136 515 430
Head Office: 19 Saggart Field Road,
MINTO NSW 2566 AUSTRALIA.
Phone: + 61 2 9827 3400
Fax: + 61 2 9603 8559
Note: In keeping with our policy of continuing product improvement, we reserve the right to alter specifications without notice.
National Tel. Hotline: 1300 550 116. National Fax. Hotline: 1300 550 115.
Designed for your peace of mind and protection.
1300 Watts ‐ 10 Amps
10 Amps
6.75 Amps
1.1 Kw
280
2025 x 800 x 1650mm
330
2.673m³
Average Airflow Inflow to grille
Velocity Downflow
Airflow Velocity Detemined in Inflow
accordance with AS 1807.1
Downflow
Exhaust
AS: 1807.20
Downflow
1300 Watts ‐ 10 Amps
10 Amps
4.5 Amps
0.7 Kw
250
1400 x 800 x 1650mm
280
1.848m³
Sound Emission
HEPA Filter
Typical Efficiency Exhaust
Germicidal UV Lamp AS 1807.23
Fluorescent Lamp Intensity AS 1807.15
Certification to Australian Standards
Main Body
Cabinet Construction Work Surface
AS 2252.2 ‐ 2009 Side Walls and Sump
Gas Tightness of outer Shell
Front Viewing Window
Electrical Cabinet Power / Amp
220‐240V AC Outlet Amp Fuse
50Hz Full Load Amps
Complies to AS 3100 Power Consumption
Cabinet Net Weight Kg
Shipping Dimensions External (W x D x H)
Total Shipping Weight Kg
Total Shipping Volume
Clyde Apac®