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GENES and DNA
About me
• DEEPAK SANTOSH KUMAR
• B Pharm (Mahatma Jyotiba Phule Rohilkhand University, Bareilly, UP)
• M Pharm (IIT-BHU, Varanasi, UP)
• Qualified GPAT 2017, 2018, 2019
• Qualified CUCET 2018
• Qualified NIPER JEE (523 AIR)
• Offered job as “Pharmacovigilance Associate” at APCER LIFE SCIENCES,
Ahmedabad, Gujarat during M Pharm
GENES?
It is the sequence of DNA nucleotides that contain the information for the production of an ordered amino
acid sequence for a single polypeptide chain.
There are present along the length of the chromosomes
Each gene contains information in code that allows the cell to make (almost always) a specific protein, the so-
called gene product.
Each gene codes for one specific protein or 1 Gene = 1 Protein
Number of genes in the human genome is approximately between 25000 and 30000
Genes normally exist in pairs, because the gene on one chromosome is matched at the equivalent site (locus)
on the other chromosome of the pair
DNA?
Genes are composed mainly of very long strands of DNA
The total length of DNA in each cell is about a metre
DNA is a double-stranded molecule, made up of two chains of nucleotides
Nucleotides consist of three subunits like
• a sugar
• a phosphate group
• a base (bases are linked to the sugars)
Nucleosides contain a sugar linked to a nitrogen-containing base
In DNA, the sugar is deoxyribose
Each base binds to another base on the other sugar/phosphate chain
The two chains are twisted around one another, giving a double helix arrangement
The double helix itself is further twisted and wrapped in a highly organised way around structural proteins called
histones, which are important in maintaining the heavily coiled three-dimensional shape of the DNA.
How a nucleotide is formed from nucleoside?
• The physiologically important bases, purines and pyrimidines, have
ring structures
• These structures are bound to ribose or 2-deoxyribose to complete
the “Nucleoside”
• When inorganic phosphate is added to the nucleoside, a nucleotide
is formed
• Nucleosides and nucleotides form the backbone for RNA and DNA,
as well as a variety of coenzymes and regulatory molecules of
physiologic importance (e.g., NAD+, NADP+, and ATP)
• The purines and pyrimidines released by the breakdown of
nucleotides may be reused or catabolized. Minor amounts are
excreted unchanged in the urine.
DNA
DNA + Histone proteins = Chromatin
DNA carries a huge amount of information that determines all
biological activities of an organism, and which is transmitted from
one generation to the next
The key to how this information is kept is found in the bases
within DNA i.e. Adenine, Guanine, Cytosine, Thymine
Bases are arranged in a precise order along the DNA molecule,
making a base code that can be read when protein
synthesis is required
Each base along one strand of DNA pairs with a base on the other
strand in a precise and predictable way. This is known as
complementary base pairing
Adenine always pairs with thymine (and vice versa), and cytosine
and guanine always go together
MITOCHONDRIAL DNA?
Each body cell has, on average, 5000 mitochondria
Mitochondria hold a quantity of DNA (mitochondrial DNA), which codes, for example, for enzymes important in
energy production
This DNA (mtDNA) is passed from one generation to another via the ovum, so the offspring’s complement of
mitochondrial DNA is inherited from the mother.
Certain rare inherited disorders that arise from faulty mitochondrial DNA are therefore passed through
generations via the maternal line.

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Genes and DNA

  • 2. About me • DEEPAK SANTOSH KUMAR • B Pharm (Mahatma Jyotiba Phule Rohilkhand University, Bareilly, UP) • M Pharm (IIT-BHU, Varanasi, UP) • Qualified GPAT 2017, 2018, 2019 • Qualified CUCET 2018 • Qualified NIPER JEE (523 AIR) • Offered job as “Pharmacovigilance Associate” at APCER LIFE SCIENCES, Ahmedabad, Gujarat during M Pharm
  • 3. GENES? It is the sequence of DNA nucleotides that contain the information for the production of an ordered amino acid sequence for a single polypeptide chain. There are present along the length of the chromosomes Each gene contains information in code that allows the cell to make (almost always) a specific protein, the so- called gene product. Each gene codes for one specific protein or 1 Gene = 1 Protein Number of genes in the human genome is approximately between 25000 and 30000 Genes normally exist in pairs, because the gene on one chromosome is matched at the equivalent site (locus) on the other chromosome of the pair
  • 4. DNA? Genes are composed mainly of very long strands of DNA The total length of DNA in each cell is about a metre DNA is a double-stranded molecule, made up of two chains of nucleotides Nucleotides consist of three subunits like • a sugar • a phosphate group • a base (bases are linked to the sugars) Nucleosides contain a sugar linked to a nitrogen-containing base In DNA, the sugar is deoxyribose Each base binds to another base on the other sugar/phosphate chain The two chains are twisted around one another, giving a double helix arrangement The double helix itself is further twisted and wrapped in a highly organised way around structural proteins called histones, which are important in maintaining the heavily coiled three-dimensional shape of the DNA.
  • 5. How a nucleotide is formed from nucleoside? • The physiologically important bases, purines and pyrimidines, have ring structures • These structures are bound to ribose or 2-deoxyribose to complete the “Nucleoside” • When inorganic phosphate is added to the nucleoside, a nucleotide is formed • Nucleosides and nucleotides form the backbone for RNA and DNA, as well as a variety of coenzymes and regulatory molecules of physiologic importance (e.g., NAD+, NADP+, and ATP) • The purines and pyrimidines released by the breakdown of nucleotides may be reused or catabolized. Minor amounts are excreted unchanged in the urine.
  • 6. DNA DNA + Histone proteins = Chromatin DNA carries a huge amount of information that determines all biological activities of an organism, and which is transmitted from one generation to the next The key to how this information is kept is found in the bases within DNA i.e. Adenine, Guanine, Cytosine, Thymine Bases are arranged in a precise order along the DNA molecule, making a base code that can be read when protein synthesis is required Each base along one strand of DNA pairs with a base on the other strand in a precise and predictable way. This is known as complementary base pairing Adenine always pairs with thymine (and vice versa), and cytosine and guanine always go together
  • 7. MITOCHONDRIAL DNA? Each body cell has, on average, 5000 mitochondria Mitochondria hold a quantity of DNA (mitochondrial DNA), which codes, for example, for enzymes important in energy production This DNA (mtDNA) is passed from one generation to another via the ovum, so the offspring’s complement of mitochondrial DNA is inherited from the mother. Certain rare inherited disorders that arise from faulty mitochondrial DNA are therefore passed through generations via the maternal line.